Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS02515 and PFLU_RS02520 are separated by 63 nucleotides PFLU_RS02520 and PFLU_RS02525 are separated by 286 nucleotides PFLU_RS02525 and PFLU_RS02530 are separated by 108 nucleotides PFLU_RS02530 and PFLU_RS02535 are separated by 183 nucleotides
PFLU_RS02515: PFLU_RS02515 - flagellar motor protein MotB, at 578,813 to 579,832
_RS02515
PFLU_RS02520: PFLU_RS02520 - hypothetical protein, at 579,896 to 580,291
_RS02520
PFLU_RS02525: PFLU_RS02525 - small ribosomal subunit biogenesis GTPase RsgA, at 580,578 to 581,609
_RS02525
PFLU_RS02530: PFLU_RS02530 - oligoribonuclease, at 581,718 to 582,260
_RS02530
PFLU_RS02535: PFLU_RS02535 - trimeric intracellular cation channel family protein, at 582,444 to 583,058
_RS02535
Position (kb) 580
581
582 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 579.808 kb on + strand at 579.809 kb on - strand at 579.809 kb on - strand at 579.831 kb on - strand at 579.842 kb on + strand at 579.842 kb on + strand at 579.842 kb on + strand at 579.842 kb on + strand at 579.842 kb on + strand at 579.842 kb on + strand at 579.842 kb on + strand at 579.842 kb on + strand at 579.843 kb on - strand at 579.843 kb on - strand at 579.843 kb on - strand at 579.843 kb on - strand at 579.844 kb on + strand at 579.845 kb on - strand at 579.845 kb on - strand at 579.858 kb on + strand at 579.858 kb on + strand at 579.909 kb on + strand at 579.909 kb on + strand at 579.909 kb on + strand at 579.909 kb on + strand at 579.909 kb on + strand at 579.910 kb on - strand at 579.910 kb on - strand at 579.969 kb on + strand, within PFLU_RS02520 at 580.030 kb on + strand, within PFLU_RS02520 at 580.031 kb on - strand, within PFLU_RS02520 at 580.031 kb on - strand, within PFLU_RS02520 at 580.031 kb on - strand, within PFLU_RS02520 at 580.032 kb on + strand, within PFLU_RS02520 at 580.032 kb on + strand, within PFLU_RS02520 at 580.032 kb on + strand, within PFLU_RS02520 at 580.032 kb on + strand, within PFLU_RS02520 at 580.032 kb on + strand, within PFLU_RS02520 at 580.033 kb on - strand, within PFLU_RS02520 at 580.033 kb on - strand, within PFLU_RS02520 at 580.033 kb on - strand, within PFLU_RS02520 at 580.033 kb on - strand, within PFLU_RS02520 at 580.033 kb on - strand, within PFLU_RS02520 at 580.033 kb on - strand, within PFLU_RS02520 at 580.033 kb on - strand, within PFLU_RS02520 at 580.065 kb on + strand, within PFLU_RS02520 at 580.065 kb on + strand, within PFLU_RS02520 at 580.065 kb on + strand, within PFLU_RS02520 at 580.065 kb on + strand, within PFLU_RS02520 at 580.065 kb on + strand, within PFLU_RS02520 at 580.066 kb on - strand, within PFLU_RS02520 at 580.066 kb on - strand, within PFLU_RS02520 at 580.066 kb on - strand, within PFLU_RS02520 at 580.066 kb on - strand, within PFLU_RS02520 at 580.066 kb on - strand, within PFLU_RS02520 at 580.090 kb on + strand, within PFLU_RS02520 at 580.090 kb on + strand, within PFLU_RS02520 at 580.090 kb on + strand, within PFLU_RS02520 at 580.090 kb on + strand, within PFLU_RS02520 at 580.090 kb on + strand, within PFLU_RS02520 at 580.091 kb on - strand, within PFLU_RS02520 at 580.094 kb on + strand, within PFLU_RS02520 at 580.094 kb on + strand, within PFLU_RS02520 at 580.094 kb on + strand, within PFLU_RS02520 at 580.094 kb on + strand, within PFLU_RS02520 at 580.094 kb on + strand, within PFLU_RS02520 at 580.094 kb on + strand, within PFLU_RS02520 at 580.094 kb on + strand, within PFLU_RS02520 at 580.094 kb on + strand, within PFLU_RS02520 at 580.094 kb on + strand, within PFLU_RS02520 at 580.094 kb on + strand, within PFLU_RS02520 at 580.094 kb on + strand, within PFLU_RS02520 at 580.095 kb on - strand, within PFLU_RS02520 at 580.095 kb on - strand, within PFLU_RS02520 at 580.095 kb on - strand, within PFLU_RS02520 at 580.095 kb on - strand, within PFLU_RS02520 at 580.095 kb on - strand, within PFLU_RS02520 at 580.118 kb on + strand, within PFLU_RS02520 at 580.118 kb on + strand, within PFLU_RS02520 at 580.118 kb on + strand, within PFLU_RS02520 at 580.119 kb on - strand, within PFLU_RS02520 at 580.119 kb on - strand, within PFLU_RS02520 at 580.119 kb on - strand, within PFLU_RS02520 at 580.132 kb on + strand, within PFLU_RS02520 at 580.133 kb on - strand, within PFLU_RS02520 at 580.133 kb on - strand, within PFLU_RS02520 at 580.133 kb on - strand, within PFLU_RS02520 at 580.133 kb on - strand, within PFLU_RS02520 at 580.256 kb on + strand at 580.256 kb on + strand at 580.256 kb on + strand at 580.256 kb on + strand at 580.256 kb on + strand at 580.256 kb on + strand at 580.256 kb on + strand at 580.256 kb on + strand at 580.257 kb on - strand at 580.257 kb on - strand at 580.257 kb on - strand at 580.257 kb on - strand at 580.257 kb on - strand at 580.257 kb on - strand at 580.257 kb on - strand at 580.257 kb on - strand at 580.257 kb on - strand at 580.257 kb on - strand at 580.257 kb on - strand at 580.257 kb on - strand at 580.257 kb on - strand at 580.278 kb on + strand at 580.278 kb on + strand at 580.278 kb on + strand at 580.278 kb on + strand at 580.278 kb on + strand at 580.278 kb on + strand at 580.278 kb on + strand at 580.279 kb on - strand at 580.279 kb on - strand at 580.279 kb on - strand at 580.279 kb on - strand at 580.279 kb on - strand at 580.279 kb on - strand at 580.281 kb on + strand at 580.281 kb on + strand at 580.281 kb on + strand at 580.282 kb on - strand at 580.282 kb on - strand at 580.282 kb on - strand at 580.436 kb on + strand at 580.436 kb on + strand at 580.437 kb on - strand at 580.437 kb on - strand at 580.437 kb on - strand at 580.437 kb on - strand at 580.533 kb on + strand at 580.534 kb on - strand at 580.555 kb on + strand at 580.555 kb on + strand at 580.555 kb on + strand at 580.555 kb on + strand at 580.555 kb on + strand at 580.555 kb on + strand at 580.555 kb on + strand at 580.555 kb on + strand at 580.555 kb on + strand at 580.555 kb on + strand at 580.555 kb on + strand at 580.555 kb on + strand at 580.555 kb on + strand at 580.555 kb on + strand at 580.555 kb on + strand at 580.556 kb on - strand at 580.556 kb on - strand at 580.556 kb on - strand at 580.556 kb on - strand at 580.556 kb on - strand at 580.556 kb on - strand at 580.556 kb on - strand at 580.556 kb on - strand at 580.556 kb on - strand at 580.557 kb on + strand at 580.557 kb on + strand at 580.557 kb on + strand at 580.557 kb on + strand at 580.557 kb on + strand at 580.557 kb on + strand at 580.557 kb on + strand at 580.558 kb on - strand at 580.558 kb on - strand at 580.558 kb on - strand at 580.558 kb on - strand at 580.558 kb on - strand at 580.558 kb on - strand at 580.558 kb on - strand at 580.558 kb on - strand at 580.558 kb on - strand at 580.558 kb on - strand at 580.558 kb on - strand at 580.558 kb on - strand at 580.582 kb on + strand at 580.607 kb on + strand at 580.608 kb on - strand at 580.608 kb on - strand at 580.622 kb on + strand at 580.622 kb on + strand at 580.622 kb on + strand at 580.622 kb on + strand at 580.622 kb on + strand at 580.622 kb on + strand at 580.622 kb on + strand at 580.622 kb on + strand at 580.622 kb on + strand at 580.622 kb on + strand at 580.623 kb on - strand at 580.623 kb on - strand at 580.623 kb on - strand at 580.623 kb on - strand at 580.943 kb on + strand, within PFLU_RS02525 at 580.944 kb on - strand, within PFLU_RS02525 at 580.976 kb on + strand, within PFLU_RS02525 at 580.976 kb on + strand, within PFLU_RS02525 at 580.976 kb on + strand, within PFLU_RS02525 at 580.976 kb on + strand, within PFLU_RS02525 at 580.976 kb on + strand, within PFLU_RS02525 at 580.977 kb on - strand, within PFLU_RS02525 at 580.977 kb on - strand, within PFLU_RS02525 at 580.977 kb on - strand, within PFLU_RS02525 at 580.977 kb on - strand, within PFLU_RS02525 at 580.977 kb on - strand, within PFLU_RS02525 at 581.343 kb on + strand, within PFLU_RS02525 at 581.638 kb on + strand at 581.639 kb on - strand at 581.639 kb on - strand at 581.642 kb on + strand at 581.643 kb on - strand at 581.643 kb on - strand at 581.659 kb on + strand at 581.659 kb on + strand at 581.660 kb on - strand at 581.677 kb on + strand at 581.691 kb on - strand at 582.411 kb on + strand at 582.411 kb on + strand at 582.411 kb on + strand at 582.411 kb on + strand at 582.412 kb on - strand at 582.412 kb on - strand at 582.412 kb on - strand at 582.459 kb on + strand at 582.459 kb on + strand at 582.460 kb on - strand at 582.460 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 579,808 + -1.0 579,809 - +0.4 579,809 - +1.2 579,831 - +0.8 579,842 + -0.4 579,842 + -0.2 579,842 + -1.2 579,842 + -0.5 579,842 + +0.7 579,842 + +1.5 579,842 + +1.1 579,842 + +1.1 579,843 - -1.9 579,843 - +1.1 579,843 - -0.3 579,843 - -0.8 579,844 + -0.3 579,845 - -0.0 579,845 - +0.6 579,858 + +0.2 579,858 + +0.7 579,909 + +0.4 579,909 + -1.2 579,909 + -0.7 579,909 + +0.5 579,909 + -1.0 579,910 - -0.9 579,910 - +0.7 579,969 + PFLU_RS02520 0.18 -0.0 580,030 + PFLU_RS02520 0.34 +0.2 580,031 - PFLU_RS02520 0.34 -0.4 580,031 - PFLU_RS02520 0.34 -2.7 580,031 - PFLU_RS02520 0.34 +0.7 580,032 + PFLU_RS02520 0.34 -1.2 580,032 + PFLU_RS02520 0.34 +1.3 580,032 + PFLU_RS02520 0.34 -0.1 580,032 + PFLU_RS02520 0.34 -0.7 580,032 + PFLU_RS02520 0.34 +0.7 580,033 - PFLU_RS02520 0.35 -0.7 580,033 - PFLU_RS02520 0.35 -1.3 580,033 - PFLU_RS02520 0.35 +0.5 580,033 - PFLU_RS02520 0.35 +1.0 580,033 - PFLU_RS02520 0.35 +0.0 580,033 - PFLU_RS02520 0.35 +0.6 580,033 - PFLU_RS02520 0.35 -1.6 580,065 + PFLU_RS02520 0.43 +0.3 580,065 + PFLU_RS02520 0.43 +0.2 580,065 + PFLU_RS02520 0.43 -0.2 580,065 + PFLU_RS02520 0.43 -0.2 580,065 + PFLU_RS02520 0.43 +1.0 580,066 - PFLU_RS02520 0.43 -0.0 580,066 - PFLU_RS02520 0.43 -0.5 580,066 - PFLU_RS02520 0.43 +1.0 580,066 - PFLU_RS02520 0.43 +1.0 580,066 - PFLU_RS02520 0.43 -1.1 580,090 + PFLU_RS02520 0.49 -2.8 580,090 + PFLU_RS02520 0.49 -0.2 580,090 + PFLU_RS02520 0.49 +1.1 580,090 + PFLU_RS02520 0.49 -0.5 580,090 + PFLU_RS02520 0.49 -0.5 580,091 - PFLU_RS02520 0.49 +0.7 580,094 + PFLU_RS02520 0.50 +2.3 580,094 + PFLU_RS02520 0.50 -2.0 580,094 + PFLU_RS02520 0.50 +1.0 580,094 + PFLU_RS02520 0.50 +1.2 580,094 + PFLU_RS02520 0.50 -0.5 580,094 + PFLU_RS02520 0.50 -0.0 580,094 + PFLU_RS02520 0.50 -0.4 580,094 + PFLU_RS02520 0.50 -0.0 580,094 + PFLU_RS02520 0.50 -0.6 580,094 + PFLU_RS02520 0.50 +0.9 580,094 + PFLU_RS02520 0.50 +1.8 580,095 - PFLU_RS02520 0.50 -1.0 580,095 - PFLU_RS02520 0.50 -1.3 580,095 - PFLU_RS02520 0.50 +1.4 580,095 - PFLU_RS02520 0.50 -0.4 580,095 - PFLU_RS02520 0.50 +0.0 580,118 + PFLU_RS02520 0.56 -0.3 580,118 + PFLU_RS02520 0.56 -0.9 580,118 + PFLU_RS02520 0.56 +1.7 580,119 - PFLU_RS02520 0.56 +0.3 580,119 - PFLU_RS02520 0.56 -0.7 580,119 - PFLU_RS02520 0.56 +0.3 580,132 + PFLU_RS02520 0.60 +0.0 580,133 - PFLU_RS02520 0.60 -0.5 580,133 - PFLU_RS02520 0.60 +0.2 580,133 - PFLU_RS02520 0.60 -0.4 580,133 - PFLU_RS02520 0.60 -0.2 580,256 + -0.3 580,256 + -0.2 580,256 + +0.2 580,256 + -0.6 580,256 + +0.4 580,256 + +0.4 580,256 + +0.1 580,256 + -0.3 580,257 - +0.4 580,257 - -0.3 580,257 - +0.9 580,257 - +1.5 580,257 - +0.1 580,257 - +2.3 580,257 - +0.1 580,257 - -1.6 580,257 - +0.1 580,257 - -0.3 580,257 - -1.3 580,257 - -1.0 580,257 - +1.4 580,278 + +0.8 580,278 + -1.5 580,278 + -0.8 580,278 + -1.7 580,278 + -1.5 580,278 + -0.4 580,278 + -1.8 580,279 - -0.4 580,279 - -0.8 580,279 - +1.0 580,279 - -0.5 580,279 - -1.8 580,279 - -0.9 580,281 + +0.7 580,281 + -0.0 580,281 + +0.0 580,282 - +0.8 580,282 - -0.6 580,282 - -1.0 580,436 + +0.8 580,436 + +1.1 580,437 - -0.6 580,437 - +0.1 580,437 - -0.8 580,437 - -0.5 580,533 + -0.7 580,534 - +0.0 580,555 + +0.2 580,555 + +0.8 580,555 + +1.7 580,555 + +0.1 580,555 + -0.7 580,555 + +1.1 580,555 + +0.4 580,555 + +0.0 580,555 + +1.3 580,555 + +0.3 580,555 + +0.0 580,555 + +1.7 580,555 + -0.1 580,555 + +0.3 580,555 + -1.0 580,556 - -2.5 580,556 - -0.9 580,556 - +0.2 580,556 - -0.9 580,556 - -0.9 580,556 - -0.5 580,556 - +0.7 580,556 - -1.5 580,556 - +2.1 580,557 + +0.9 580,557 + +0.1 580,557 + -1.3 580,557 + -0.2 580,557 + +0.4 580,557 + +0.3 580,557 + +0.2 580,558 - -0.2 580,558 - +1.0 580,558 - +0.1 580,558 - -0.3 580,558 - -0.6 580,558 - +0.7 580,558 - +2.3 580,558 - -1.2 580,558 - +0.2 580,558 - +2.7 580,558 - -0.6 580,558 - +0.4 580,582 + +1.7 580,607 + -0.3 580,608 - -1.1 580,608 - +0.0 580,622 + -0.3 580,622 + +2.3 580,622 + +2.3 580,622 + -0.5 580,622 + +0.7 580,622 + +1.3 580,622 + +2.3 580,622 + +1.3 580,622 + +2.3 580,622 + +2.3 580,623 - +0.7 580,623 - +0.3 580,623 - +0.7 580,623 - +0.3 580,943 + PFLU_RS02525 0.35 +2.3 580,944 - PFLU_RS02525 0.35 +2.3 580,976 + PFLU_RS02525 0.39 +2.3 580,976 + PFLU_RS02525 0.39 +3.3 580,976 + PFLU_RS02525 0.39 +1.0 580,976 + PFLU_RS02525 0.39 +1.7 580,976 + PFLU_RS02525 0.39 +1.3 580,977 - PFLU_RS02525 0.39 -0.3 580,977 - PFLU_RS02525 0.39 +0.7 580,977 - PFLU_RS02525 0.39 +1.3 580,977 - PFLU_RS02525 0.39 +0.7 580,977 - PFLU_RS02525 0.39 +0.7 581,343 + PFLU_RS02525 0.74 -0.0 581,638 + +1.5 581,639 - +0.2 581,639 - +0.4 581,642 + -0.7 581,643 - +0.7 581,643 - -0.1 581,659 + +1.4 581,659 + +1.6 581,660 - +1.5 581,677 + +0.5 581,691 - +0.2 582,411 + -1.2 582,411 + +0.9 582,411 + -2.4 582,411 + -0.9 582,412 - +0.4 582,412 - -0.6 582,412 - +1.8 582,459 + -1.5 582,459 + +1.4 582,460 - -1.6 582,460 - +0.6
Or see this region's nucleotide sequence