Experiment: A22 0.16 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16570 and PS417_16575 are separated by 657 nucleotides PS417_16575 and PS417_16580 are separated by 10 nucleotides PS417_16580 and PS417_16585 are separated by 72 nucleotides
PS417_16570: PS417_16570 - isocitrate lyase, at 3,620,632 to 3,621,957
_16570
PS417_16575: PS417_16575 - NADH-quinone oxidoreductase subunit A, at 3,622,615 to 3,623,028
_16575
PS417_16580: PS417_16580 - NADH dehydrogenase, at 3,623,039 to 3,623,713
_16580
PS417_16585: PS417_16585 - NADH:ubiquinone oxidoreductase, at 3,623,786 to 3,625,570
_16585
Position (kb)
3622
3623
3624 Strain fitness (log2 ratio)
-1
0
1 at 3621.689 kb on - strand, within PS417_16570 at 3621.830 kb on - strand at 3621.869 kb on - strand at 3621.885 kb on + strand at 3622.271 kb on + strand at 3622.272 kb on - strand at 3622.272 kb on - strand at 3622.291 kb on - strand at 3622.430 kb on + strand at 3622.498 kb on + strand at 3622.513 kb on + strand at 3622.513 kb on + strand at 3622.550 kb on + strand at 3622.550 kb on + strand at 3622.551 kb on - strand at 3622.567 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.16 mg/ml remove 3,621,689 - PS417_16570 0.80 -0.7 3,621,830 - +0.3 3,621,869 - +0.1 3,621,885 + +0.1 3,622,271 + -0.0 3,622,272 - +0.5 3,622,272 - -0.5 3,622,291 - -0.2 3,622,430 + +1.6 3,622,498 + +0.1 3,622,513 + +1.0 3,622,513 + -0.1 3,622,550 + +0.6 3,622,550 + -0.2 3,622,551 - -1.0 3,622,567 + +0.1
Or see this region's nucleotide sequence