Strain Fitness in Escherichia coli BW25113 around b2557

Experiment: L-Fucose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfhK and glmY are separated by 58 nucleotidesglmY and purL are separated by 315 nucleotidespurL and mltF are separated by 257 nucleotides b2556: yfhK - putative 2-component sensor protein (VIMSS), at 2,687,693 to 2,689,120 yfhK b4441: glmY - sRNA activator of glmS mRNA (RefSeq), at 2,689,179 to 2,689,362 glmY b2557: purL - phosphoribosylformylglycinamidine synthase (NCBI), at 2,689,678 to 2,693,565 purL b2558: mltF - predicted periplasmic binding protein/transglycosylase (RefSeq), at 2,693,823 to 2,695,379 mltF Position (kb) 2689 2690 2691 2692 2693 2694Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 2688.707 kb on - strand, within yfhKat 2688.732 kb on - strand, within yfhKat 2688.874 kb on + strand, within yfhKat 2688.874 kb on + strand, within yfhKat 2688.908 kb on + strand, within yfhKat 2688.912 kb on + strand, within yfhKat 2688.914 kb on - strand, within yfhKat 2688.914 kb on - strand, within yfhKat 2688.969 kb on + strand, within yfhKat 2689.027 kb on + strandat 2689.030 kb on - strandat 2689.155 kb on - strandat 2689.169 kb on + strandat 2689.250 kb on + strand, within glmYat 2689.677 kb on - strandat 2689.716 kb on + strandat 2689.716 kb on + strandat 2689.716 kb on + strandat 2689.724 kb on - strandat 2689.838 kb on - strandat 2689.838 kb on - strandat 2689.891 kb on + strandat 2689.959 kb on + strandat 2690.045 kb on + strandat 2690.136 kb on + strand, within purLat 2690.136 kb on + strand, within purLat 2690.199 kb on - strand, within purLat 2690.199 kb on - strand, within purLat 2690.209 kb on + strand, within purLat 2690.294 kb on + strand, within purLat 2690.299 kb on + strand, within purLat 2690.308 kb on - strand, within purLat 2690.356 kb on - strand, within purLat 2690.381 kb on - strand, within purLat 2690.381 kb on - strand, within purLat 2690.430 kb on - strand, within purLat 2690.448 kb on + strand, within purLat 2690.566 kb on + strand, within purLat 2690.566 kb on + strand, within purLat 2690.718 kb on + strand, within purLat 2690.724 kb on + strand, within purLat 2690.731 kb on - strand, within purLat 2690.741 kb on - strand, within purLat 2690.836 kb on + strand, within purLat 2690.917 kb on - strand, within purLat 2690.931 kb on + strand, within purLat 2691.050 kb on - strand, within purLat 2691.235 kb on - strand, within purLat 2691.307 kb on - strand, within purLat 2691.582 kb on + strand, within purLat 2691.590 kb on - strand, within purLat 2691.685 kb on + strand, within purLat 2691.713 kb on - strand, within purLat 2691.713 kb on - strand, within purLat 2691.855 kb on - strand, within purLat 2691.860 kb on + strand, within purLat 2691.995 kb on - strand, within purLat 2692.098 kb on + strand, within purLat 2692.356 kb on + strand, within purLat 2692.367 kb on - strand, within purLat 2692.474 kb on + strand, within purLat 2692.486 kb on + strand, within purLat 2692.501 kb on - strand, within purLat 2692.519 kb on - strand, within purLat 2692.519 kb on - strand, within purLat 2692.524 kb on + strand, within purLat 2692.525 kb on - strand, within purLat 2692.525 kb on - strand, within purLat 2692.527 kb on + strand, within purLat 2692.527 kb on + strand, within purLat 2692.940 kb on + strand, within purLat 2692.973 kb on - strand, within purLat 2693.040 kb on + strand, within purLat 2693.055 kb on + strand, within purLat 2693.288 kb on - strandat 2693.315 kb on + strandat 2693.386 kb on + strandat 2693.577 kb on + strandat 2693.888 kb on - strandat 2693.888 kb on - strandat 2693.916 kb on + strandat 2693.916 kb on + strandat 2694.119 kb on + strand, within mltFat 2694.152 kb on + strand, within mltFat 2694.152 kb on + strand, within mltFat 2694.152 kb on + strand, within mltFat 2694.158 kb on - strand, within mltFat 2694.158 kb on - strand, within mltFat 2694.194 kb on + strand, within mltFat 2694.194 kb on + strand, within mltFat 2694.212 kb on - strand, within mltFat 2694.212 kb on - strand, within mltFat 2694.236 kb on + strand, within mltFat 2694.252 kb on - strand, within mltFat 2694.283 kb on - strand, within mltFat 2694.290 kb on + strand, within mltFat 2694.310 kb on - strand, within mltFat 2694.319 kb on - strand, within mltFat 2694.433 kb on - strand, within mltFat 2694.438 kb on + strand, within mltFat 2694.438 kb on + strand, within mltF

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Fucose (C)
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2,688,707 - yfhK b2556 0.71 +0.2
2,688,732 - yfhK b2556 0.73 +0.1
2,688,874 + yfhK b2556 0.83 +0.4
2,688,874 + yfhK b2556 0.83 -0.7
2,688,908 + yfhK b2556 0.85 +0.5
2,688,912 + yfhK b2556 0.85 -0.2
2,688,914 - yfhK b2556 0.86 +0.3
2,688,914 - yfhK b2556 0.86 +0.5
2,688,969 + yfhK b2556 0.89 -0.3
2,689,027 + +0.1
2,689,030 - +0.3
2,689,155 - -0.5
2,689,169 + -0.4
2,689,250 + glmY b4441 0.39 +0.3
2,689,677 - +0.2
2,689,716 + -2.4
2,689,716 + -1.2
2,689,716 + -3.8
2,689,724 - -2.4
2,689,838 - -3.3
2,689,838 - -4.8
2,689,891 + -0.4
2,689,959 + -3.1
2,690,045 + -5.2
2,690,136 + purL b2557 0.12 -1.9
2,690,136 + purL b2557 0.12 -3.3
2,690,199 - purL b2557 0.13 -1.1
2,690,199 - purL b2557 0.13 -1.6
2,690,209 + purL b2557 0.14 -4.8
2,690,294 + purL b2557 0.16 -3.1
2,690,299 + purL b2557 0.16 -1.7
2,690,308 - purL b2557 0.16 -2.0
2,690,356 - purL b2557 0.17 +1.6
2,690,381 - purL b2557 0.18 -2.3
2,690,381 - purL b2557 0.18 -1.4
2,690,430 - purL b2557 0.19 -2.7
2,690,448 + purL b2557 0.20 -3.2
2,690,566 + purL b2557 0.23 -2.2
2,690,566 + purL b2557 0.23 -1.6
2,690,718 + purL b2557 0.27 -2.0
2,690,724 + purL b2557 0.27 +0.6
2,690,731 - purL b2557 0.27 -2.1
2,690,741 - purL b2557 0.27 -2.4
2,690,836 + purL b2557 0.30 -2.7
2,690,917 - purL b2557 0.32 -2.7
2,690,931 + purL b2557 0.32 -0.0
2,691,050 - purL b2557 0.35 -1.2
2,691,235 - purL b2557 0.40 -1.0
2,691,307 - purL b2557 0.42 -3.6
2,691,582 + purL b2557 0.49 -3.5
2,691,590 - purL b2557 0.49 -2.3
2,691,685 + purL b2557 0.52 -0.9
2,691,713 - purL b2557 0.52 -2.5
2,691,713 - purL b2557 0.52 -2.4
2,691,855 - purL b2557 0.56 -2.2
2,691,860 + purL b2557 0.56 -2.1
2,691,995 - purL b2557 0.60 -2.9
2,692,098 + purL b2557 0.62 -3.1
2,692,356 + purL b2557 0.69 -1.2
2,692,367 - purL b2557 0.69 -3.3
2,692,474 + purL b2557 0.72 -3.4
2,692,486 + purL b2557 0.72 -0.4
2,692,501 - purL b2557 0.73 -0.7
2,692,519 - purL b2557 0.73 -4.1
2,692,519 - purL b2557 0.73 -2.5
2,692,524 + purL b2557 0.73 -2.0
2,692,525 - purL b2557 0.73 -2.1
2,692,525 - purL b2557 0.73 -0.6
2,692,527 + purL b2557 0.73 -3.1
2,692,527 + purL b2557 0.73 -3.2
2,692,940 + purL b2557 0.84 -2.9
2,692,973 - purL b2557 0.85 -2.5
2,693,040 + purL b2557 0.86 -4.0
2,693,055 + purL b2557 0.87 -3.1
2,693,288 - -1.0
2,693,315 + -4.3
2,693,386 + -3.7
2,693,577 + -1.8
2,693,888 - -0.2
2,693,888 - -0.6
2,693,916 + -0.0
2,693,916 + -1.2
2,694,119 + mltF b2558 0.19 +0.5
2,694,152 + mltF b2558 0.21 +0.2
2,694,152 + mltF b2558 0.21 -1.3
2,694,152 + mltF b2558 0.21 -0.6
2,694,158 - mltF b2558 0.22 +0.2
2,694,158 - mltF b2558 0.22 +0.1
2,694,194 + mltF b2558 0.24 +0.7
2,694,194 + mltF b2558 0.24 -0.0
2,694,212 - mltF b2558 0.25 -0.5
2,694,212 - mltF b2558 0.25 +0.3
2,694,236 + mltF b2558 0.27 +0.9
2,694,252 - mltF b2558 0.28 +0.8
2,694,283 - mltF b2558 0.30 -0.0
2,694,290 + mltF b2558 0.30 -0.8
2,694,310 - mltF b2558 0.31 -0.5
2,694,319 - mltF b2558 0.32 +0.0
2,694,433 - mltF b2558 0.39 -0.8
2,694,438 + mltF b2558 0.39 +0.6
2,694,438 + mltF b2558 0.39 +1.6

Or see this region's nucleotide sequence