Strain Fitness in Escherichia coli BW25113 around b3205

Experiment: L-Fucose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrpoN and yhbH are separated by 22 nucleotidesyhbH and ptsN are separated by 117 nucleotidesptsN and yhbJ are separated by 45 nucleotidesyhbJ and npr overlap by 4 nucleotidesnpr and yrbL are separated by 213 nucleotides b3202: rpoN - DNA-directed RNA polymerase subunit N (NCBI), at 3,342,739 to 3,344,172 rpoN b3203: yhbH - predicted ribosome-associated, sigma 54 modulation protein (NCBI), at 3,344,195 to 3,344,482 yhbH b3204: ptsN - sugar-specific enzyme IIA component of PTS (NCBI), at 3,344,600 to 3,345,091 ptsN b3205: yhbJ - hypothetical protein (NCBI), at 3,345,137 to 3,345,991 yhbJ b3206: npr - phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI), at 3,345,988 to 3,346,260 npr b3207: yrbL - hypothetical protein (NCBI), at 3,346,474 to 3,347,106 yrbL Position (kb) 3345 3346Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3344.190 kb on + strandat 3344.190 kb on + strandat 3344.193 kb on - strandat 3344.193 kb on - strandat 3344.195 kb on + strandat 3344.201 kb on - strandat 3344.302 kb on + strand, within yhbHat 3344.302 kb on + strand, within yhbHat 3344.310 kb on - strand, within yhbHat 3344.345 kb on + strand, within yhbHat 3344.346 kb on - strand, within yhbHat 3344.354 kb on - strand, within yhbHat 3344.354 kb on - strand, within yhbHat 3344.476 kb on + strandat 3344.502 kb on + strandat 3344.514 kb on + strandat 3344.522 kb on - strandat 3344.524 kb on - strandat 3344.524 kb on - strandat 3344.545 kb on + strandat 3344.554 kb on - strandat 3344.626 kb on + strandat 3344.636 kb on + strandat 3344.636 kb on + strandat 3344.784 kb on + strand, within ptsNat 3344.801 kb on + strand, within ptsNat 3344.810 kb on - strand, within ptsNat 3344.813 kb on + strand, within ptsNat 3344.813 kb on - strand, within ptsNat 3344.883 kb on - strand, within ptsNat 3344.914 kb on - strand, within ptsNat 3344.929 kb on + strand, within ptsNat 3344.933 kb on + strand, within ptsNat 3344.967 kb on - strand, within ptsNat 3344.967 kb on - strand, within ptsNat 3345.026 kb on + strand, within ptsNat 3345.026 kb on + strand, within ptsNat 3345.026 kb on + strand, within ptsNat 3345.034 kb on - strand, within ptsNat 3345.034 kb on - strand, within ptsNat 3345.034 kb on - strand, within ptsNat 3345.034 kb on - strand, within ptsNat 3345.066 kb on + strandat 3345.072 kb on + strandat 3345.072 kb on + strandat 3345.102 kb on - strandat 3345.102 kb on - strandat 3345.102 kb on - strandat 3345.116 kb on - strandat 3345.116 kb on - strandat 3345.139 kb on - strandat 3345.140 kb on + strandat 3345.231 kb on + strand, within yhbJat 3345.249 kb on - strand, within yhbJat 3345.264 kb on - strand, within yhbJat 3345.264 kb on - strand, within yhbJat 3345.305 kb on - strand, within yhbJat 3345.343 kb on + strand, within yhbJat 3345.396 kb on - strand, within yhbJat 3345.698 kb on + strand, within yhbJat 3345.698 kb on + strand, within yhbJat 3345.735 kb on + strand, within yhbJat 3345.863 kb on - strand, within yhbJat 3345.863 kb on - strand, within yhbJat 3345.892 kb on - strand, within yhbJat 3345.892 kb on - strand, within yhbJat 3345.909 kb on - strandat 3345.953 kb on + strandat 3346.034 kb on - strand, within nprat 3346.147 kb on + strand, within nprat 3346.173 kb on - strand, within nprat 3346.174 kb on + strand, within nprat 3346.174 kb on + strand, within nprat 3346.186 kb on - strand, within nprat 3346.196 kb on - strand, within nprat 3346.286 kb on + strandat 3346.286 kb on + strandat 3346.444 kb on - strandat 3346.487 kb on - strandat 3346.655 kb on + strand, within yrbLat 3346.688 kb on - strand, within yrbLat 3346.697 kb on - strand, within yrbLat 3346.795 kb on + strand, within yrbLat 3346.809 kb on + strand, within yrbLat 3346.921 kb on + strand, within yrbLat 3346.921 kb on + strand, within yrbLat 3346.932 kb on - strand, within yrbLat 3346.965 kb on - strand, within yrbLat 3346.972 kb on + strand, within yrbLat 3346.980 kb on - strand, within yrbLat 3346.980 kb on - strand, within yrbLat 3346.986 kb on + strand, within yrbLat 3346.986 kb on + strand, within yrbL

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Fucose (C)
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3,344,190 + +1.0
3,344,190 + +1.0
3,344,193 - -0.8
3,344,193 - -0.9
3,344,195 + +1.0
3,344,201 - -0.5
3,344,302 + yhbH b3203 0.37 +1.3
3,344,302 + yhbH b3203 0.37 +0.3
3,344,310 - yhbH b3203 0.40 +0.7
3,344,345 + yhbH b3203 0.52 +0.6
3,344,346 - yhbH b3203 0.52 +0.1
3,344,354 - yhbH b3203 0.55 -0.7
3,344,354 - yhbH b3203 0.55 -0.7
3,344,476 + +0.8
3,344,502 + +1.8
3,344,514 + +1.5
3,344,522 - -2.1
3,344,524 - +2.2
3,344,524 - -1.7
3,344,545 + +1.0
3,344,554 - -1.0
3,344,626 + -1.3
3,344,636 + +0.1
3,344,636 + -1.1
3,344,784 + ptsN b3204 0.37 -2.7
3,344,801 + ptsN b3204 0.41 -0.5
3,344,810 - ptsN b3204 0.43 -1.8
3,344,813 + ptsN b3204 0.43 -3.3
3,344,813 - ptsN b3204 0.43 -3.1
3,344,883 - ptsN b3204 0.58 -1.8
3,344,914 - ptsN b3204 0.64 -1.8
3,344,929 + ptsN b3204 0.67 -1.3
3,344,933 + ptsN b3204 0.68 -2.0
3,344,967 - ptsN b3204 0.75 -0.9
3,344,967 - ptsN b3204 0.75 -1.8
3,345,026 + ptsN b3204 0.87 -4.3
3,345,026 + ptsN b3204 0.87 +0.7
3,345,026 + ptsN b3204 0.87 -2.9
3,345,034 - ptsN b3204 0.88 -0.3
3,345,034 - ptsN b3204 0.88 -2.1
3,345,034 - ptsN b3204 0.88 -1.7
3,345,034 - ptsN b3204 0.88 -2.7
3,345,066 + -0.7
3,345,072 + +0.1
3,345,072 + -0.7
3,345,102 - -0.2
3,345,102 - -0.9
3,345,102 - +0.2
3,345,116 - +0.7
3,345,116 - -0.2
3,345,139 - -0.4
3,345,140 + -0.1
3,345,231 + yhbJ b3205 0.11 -0.0
3,345,249 - yhbJ b3205 0.13 -3.4
3,345,264 - yhbJ b3205 0.15 -0.7
3,345,264 - yhbJ b3205 0.15 +0.9
3,345,305 - yhbJ b3205 0.20 -0.8
3,345,343 + yhbJ b3205 0.24 -2.1
3,345,396 - yhbJ b3205 0.30 -1.0
3,345,698 + yhbJ b3205 0.66 -1.1
3,345,698 + yhbJ b3205 0.66 +1.2
3,345,735 + yhbJ b3205 0.70 -0.2
3,345,863 - yhbJ b3205 0.85 -1.5
3,345,863 - yhbJ b3205 0.85 -1.2
3,345,892 - yhbJ b3205 0.88 -0.7
3,345,892 - yhbJ b3205 0.88 -1.5
3,345,909 - -1.3
3,345,953 + -1.0
3,346,034 - npr b3206 0.17 +0.2
3,346,147 + npr b3206 0.58 +1.2
3,346,173 - npr b3206 0.68 -0.3
3,346,174 + npr b3206 0.68 +0.9
3,346,174 + npr b3206 0.68 +1.5
3,346,186 - npr b3206 0.73 +1.1
3,346,196 - npr b3206 0.76 +0.6
3,346,286 + +0.2
3,346,286 + +0.5
3,346,444 - -0.4
3,346,487 - +1.0
3,346,655 + yrbL b3207 0.29 -1.0
3,346,688 - yrbL b3207 0.34 +0.7
3,346,697 - yrbL b3207 0.35 +0.6
3,346,795 + yrbL b3207 0.51 +0.0
3,346,809 + yrbL b3207 0.53 +0.7
3,346,921 + yrbL b3207 0.71 +0.3
3,346,921 + yrbL b3207 0.71 +0.4
3,346,932 - yrbL b3207 0.72 -1.3
3,346,965 - yrbL b3207 0.78 -1.2
3,346,972 + yrbL b3207 0.79 -0.5
3,346,980 - yrbL b3207 0.80 +0.7
3,346,980 - yrbL b3207 0.80 +0.7
3,346,986 + yrbL b3207 0.81 +0.2
3,346,986 + yrbL b3207 0.81 -0.0

Or see this region's nucleotide sequence