Strain Fitness in Escherichia coli BW25113 around b2025

Experiment: L-Fucose (C)

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 nthisH and hisA overlap by 1 nucleotideshisA and hisF overlap by 19 nucleotideshisF and hisI overlap by 7 nucleotideshisI and wzzB are separated by 95 nucleotides b2023: hisH - imidazole glycerol phosphate synthase subunit HisH (NCBI), at 2,092,559 to 2,093,149 hisH b2024: hisA - N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS), at 2,093,149 to 2,093,886 hisA b2025: hisF - imidazole glycerol phosphate synthase subunit HisF (NCBI), at 2,093,868 to 2,094,644 hisF b2026: hisI - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein (NCBI), at 2,094,638 to 2,095,249 hisI b2027: wzzB - regulator of length of O-antigen component of lipopolysaccharide chains (VIMSS), at 2,095,345 to 2,096,325 wzzB Position (kb) 2093 2094 2095Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 2092.907 kb on + strand, within hisHat 2093.026 kb on + strand, within hisHat 2093.026 kb on + strand, within hisHat 2093.071 kb on + strand, within hisHat 2093.071 kb on + strand, within hisHat 2093.079 kb on - strand, within hisHat 2093.079 kb on - strand, within hisHat 2093.079 kb on - strand, within hisHat 2093.079 kb on - strand, within hisHat 2093.094 kb on - strandat 2093.183 kb on + strandat 2093.183 kb on + strandat 2093.454 kb on - strand, within hisAat 2093.510 kb on - strand, within hisAat 2093.818 kb on - strandat 2093.818 kb on - strandat 2093.881 kb on + strandat 2093.918 kb on + strandat 2093.978 kb on + strand, within hisFat 2094.236 kb on + strand, within hisFat 2094.236 kb on + strand, within hisFat 2094.236 kb on + strand, within hisFat 2094.244 kb on - strand, within hisFat 2094.244 kb on - strand, within hisFat 2094.248 kb on - strand, within hisFat 2094.338 kb on - strand, within hisFat 2094.596 kb on + strandat 2094.596 kb on + strandat 2094.647 kb on + strandat 2094.712 kb on - strand, within hisIat 2094.712 kb on - strand, within hisIat 2094.716 kb on + strand, within hisIat 2094.869 kb on + strand, within hisIat 2094.907 kb on - strand, within hisIat 2094.907 kb on - strand, within hisIat 2095.032 kb on + strand, within hisIat 2095.033 kb on + strand, within hisIat 2095.033 kb on + strand, within hisIat 2095.124 kb on + strand, within hisIat 2095.167 kb on + strand, within hisIat 2095.202 kb on - strandat 2095.231 kb on + strandat 2095.386 kb on - strandat 2095.490 kb on + strand, within wzzBat 2095.494 kb on + strand, within wzzBat 2095.586 kb on - strand, within wzzBat 2095.586 kb on - strand, within wzzBat 2095.606 kb on - strand, within wzzB

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction L-Fucose (C)
remove
2,092,907 + hisH b2023 0.59 -1.4
2,093,026 + hisH b2023 0.79 -1.7
2,093,026 + hisH b2023 0.79 -2.9
2,093,071 + hisH b2023 0.87 -1.5
2,093,071 + hisH b2023 0.87 -3.4
2,093,079 - hisH b2023 0.88 -4.3
2,093,079 - hisH b2023 0.88 -2.5
2,093,079 - hisH b2023 0.88 -3.3
2,093,079 - hisH b2023 0.88 -2.3
2,093,094 - -2.7
2,093,183 + -5.0
2,093,183 + -1.0
2,093,454 - hisA b2024 0.41 -3.0
2,093,510 - hisA b2024 0.49 -5.5
2,093,818 - -4.1
2,093,818 - -1.1
2,093,881 + -3.4
2,093,918 + -2.6
2,093,978 + hisF b2025 0.14 -2.2
2,094,236 + hisF b2025 0.47 -3.7
2,094,236 + hisF b2025 0.47 -3.5
2,094,236 + hisF b2025 0.47 -2.8
2,094,244 - hisF b2025 0.48 -3.0
2,094,244 - hisF b2025 0.48 -3.3
2,094,248 - hisF b2025 0.49 -1.8
2,094,338 - hisF b2025 0.60 -2.1
2,094,596 + -1.7
2,094,596 + -3.4
2,094,647 + -0.6
2,094,712 - hisI b2026 0.12 -4.0
2,094,712 - hisI b2026 0.12 -2.6
2,094,716 + hisI b2026 0.13 -3.7
2,094,869 + hisI b2026 0.38 -1.2
2,094,907 - hisI b2026 0.44 -3.6
2,094,907 - hisI b2026 0.44 -0.6
2,095,032 + hisI b2026 0.64 -3.0
2,095,033 + hisI b2026 0.65 -2.0
2,095,033 + hisI b2026 0.65 -3.3
2,095,124 + hisI b2026 0.79 -2.5
2,095,167 + hisI b2026 0.86 -3.4
2,095,202 - -3.3
2,095,231 + +0.1
2,095,386 - +0.3
2,095,490 + wzzB b2027 0.15 +0.9
2,095,494 + wzzB b2027 0.15 -0.0
2,095,586 - wzzB b2027 0.25 +0.3
2,095,586 - wzzB b2027 0.25 +0.0
2,095,606 - wzzB b2027 0.27 +0.4

Or see this region's nucleotide sequence