Strain Fitness in Escherichia coli BW25113 around b2023

Experiment: L-Fucose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisB and hisH overlap by 1 nucleotideshisH and hisA overlap by 1 nucleotideshisA and hisF overlap by 19 nucleotides b2022: hisB - imidazoleglycerolphosphate dehydratase and histidinol-phosphate phosphatase (VIMSS), at 2,091,492 to 2,092,559 hisB b2023: hisH - imidazole glycerol phosphate synthase subunit HisH (NCBI), at 2,092,559 to 2,093,149 hisH b2024: hisA - N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS), at 2,093,149 to 2,093,886 hisA b2025: hisF - imidazole glycerol phosphate synthase subunit HisF (NCBI), at 2,093,868 to 2,094,644 hisF Position (kb) 2092 2093 2094Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 2091.593 kb on - strandat 2091.593 kb on - strandat 2091.619 kb on - strand, within hisBat 2091.659 kb on + strand, within hisBat 2091.985 kb on - strand, within hisBat 2092.013 kb on - strand, within hisBat 2092.013 kb on - strand, within hisBat 2092.070 kb on + strand, within hisBat 2092.080 kb on + strand, within hisBat 2092.114 kb on - strand, within hisBat 2092.160 kb on + strand, within hisBat 2092.171 kb on - strand, within hisBat 2092.193 kb on - strand, within hisBat 2092.193 kb on - strand, within hisBat 2092.355 kb on + strand, within hisBat 2092.355 kb on + strand, within hisBat 2092.359 kb on - strand, within hisBat 2092.406 kb on + strand, within hisBat 2092.406 kb on + strand, within hisBat 2092.412 kb on + strand, within hisBat 2092.412 kb on + strand, within hisBat 2092.464 kb on + strandat 2092.533 kb on - strandat 2092.549 kb on - strandat 2092.752 kb on - strand, within hisHat 2092.772 kb on - strand, within hisHat 2092.907 kb on + strand, within hisHat 2093.026 kb on + strand, within hisHat 2093.026 kb on + strand, within hisHat 2093.071 kb on + strand, within hisHat 2093.071 kb on + strand, within hisHat 2093.079 kb on - strand, within hisHat 2093.079 kb on - strand, within hisHat 2093.079 kb on - strand, within hisHat 2093.079 kb on - strand, within hisHat 2093.094 kb on - strandat 2093.183 kb on + strandat 2093.183 kb on + strandat 2093.454 kb on - strand, within hisAat 2093.510 kb on - strand, within hisAat 2093.818 kb on - strandat 2093.818 kb on - strandat 2093.881 kb on + strandat 2093.918 kb on + strandat 2093.978 kb on + strand, within hisF

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Fucose (C)
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2,091,593 - +1.3
2,091,593 - -2.0
2,091,619 - hisB b2022 0.12 -4.1
2,091,659 + hisB b2022 0.16 -3.5
2,091,985 - hisB b2022 0.46 -4.1
2,092,013 - hisB b2022 0.49 -2.6
2,092,013 - hisB b2022 0.49 -2.6
2,092,070 + hisB b2022 0.54 -2.9
2,092,080 + hisB b2022 0.55 -2.1
2,092,114 - hisB b2022 0.58 -2.8
2,092,160 + hisB b2022 0.63 -2.6
2,092,171 - hisB b2022 0.64 -2.6
2,092,193 - hisB b2022 0.66 -3.1
2,092,193 - hisB b2022 0.66 -2.9
2,092,355 + hisB b2022 0.81 -3.5
2,092,355 + hisB b2022 0.81 -2.8
2,092,359 - hisB b2022 0.81 -3.8
2,092,406 + hisB b2022 0.86 -1.2
2,092,406 + hisB b2022 0.86 -4.6
2,092,412 + hisB b2022 0.86 -1.9
2,092,412 + hisB b2022 0.86 -2.7
2,092,464 + -3.4
2,092,533 - -4.1
2,092,549 - -1.7
2,092,752 - hisH b2023 0.33 -2.0
2,092,772 - hisH b2023 0.36 -2.6
2,092,907 + hisH b2023 0.59 -1.4
2,093,026 + hisH b2023 0.79 -1.7
2,093,026 + hisH b2023 0.79 -2.9
2,093,071 + hisH b2023 0.87 -1.5
2,093,071 + hisH b2023 0.87 -3.4
2,093,079 - hisH b2023 0.88 -4.3
2,093,079 - hisH b2023 0.88 -2.5
2,093,079 - hisH b2023 0.88 -3.3
2,093,079 - hisH b2023 0.88 -2.3
2,093,094 - -2.7
2,093,183 + -5.0
2,093,183 + -1.0
2,093,454 - hisA b2024 0.41 -3.0
2,093,510 - hisA b2024 0.49 -5.5
2,093,818 - -4.1
2,093,818 - -1.1
2,093,881 + -3.4
2,093,918 + -2.6
2,093,978 + hisF b2025 0.14 -2.2

Or see this region's nucleotide sequence