Strain Fitness in Escherichia coli BW25113 around b1854

Experiment: L-Fucose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyebK and pykA are separated by 127 nucleotidespykA and lpxM are separated by 130 nucleotides b1853: yebK - predicted DNA-binding transcriptional regulator (NCBI), at 1,934,676 to 1,935,545 yebK b1854: pykA - pyruvate kinase (NCBI), at 1,935,673 to 1,937,115 pykA b1855: lpxM - lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI), at 1,937,246 to 1,938,217 lpxM Position (kb) 1935 1936 1937 1938Strain fitness (log2 ratio) -2 -1 0 1 2at 1934.818 kb on + strand, within yebKat 1934.893 kb on + strand, within yebKat 1935.346 kb on - strand, within yebKat 1935.359 kb on + strand, within yebKat 1935.869 kb on - strand, within pykAat 1935.871 kb on + strand, within pykAat 1935.919 kb on - strand, within pykAat 1935.919 kb on - strand, within pykAat 1935.962 kb on + strand, within pykAat 1935.962 kb on + strand, within pykAat 1935.979 kb on + strand, within pykAat 1936.028 kb on + strand, within pykAat 1936.028 kb on + strand, within pykAat 1936.223 kb on - strand, within pykAat 1936.241 kb on - strand, within pykAat 1936.241 kb on - strand, within pykAat 1936.292 kb on - strand, within pykAat 1936.299 kb on - strand, within pykAat 1936.412 kb on - strand, within pykAat 1936.581 kb on - strand, within pykAat 1936.581 kb on - strand, within pykAat 1936.802 kb on - strand, within pykAat 1936.805 kb on + strand, within pykAat 1936.823 kb on - strand, within pykAat 1936.823 kb on - strand, within pykAat 1937.235 kb on - strandat 1937.235 kb on - strandat 1937.580 kb on - strand, within lpxMat 1937.674 kb on + strand, within lpxMat 1937.696 kb on + strand, within lpxMat 1937.696 kb on + strand, within lpxMat 1937.738 kb on - strand, within lpxMat 1937.742 kb on + strand, within lpxMat 1937.844 kb on + strand, within lpxMat 1937.989 kb on + strand, within lpxMat 1937.997 kb on + strand, within lpxMat 1938.027 kb on - strand, within lpxM

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Fucose (C)
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1,934,818 + yebK b1853 0.16 +1.2
1,934,893 + yebK b1853 0.25 -0.8
1,935,346 - yebK b1853 0.77 +1.3
1,935,359 + yebK b1853 0.79 +0.7
1,935,869 - pykA b1854 0.14 +0.3
1,935,871 + pykA b1854 0.14 -0.9
1,935,919 - pykA b1854 0.17 -0.9
1,935,919 - pykA b1854 0.17 -0.3
1,935,962 + pykA b1854 0.20 -0.1
1,935,962 + pykA b1854 0.20 -0.4
1,935,979 + pykA b1854 0.21 -0.2
1,936,028 + pykA b1854 0.25 +0.4
1,936,028 + pykA b1854 0.25 -0.3
1,936,223 - pykA b1854 0.38 -0.4
1,936,241 - pykA b1854 0.39 -0.5
1,936,241 - pykA b1854 0.39 -0.1
1,936,292 - pykA b1854 0.43 -1.4
1,936,299 - pykA b1854 0.43 -1.5
1,936,412 - pykA b1854 0.51 -0.4
1,936,581 - pykA b1854 0.63 +0.1
1,936,581 - pykA b1854 0.63 -0.9
1,936,802 - pykA b1854 0.78 -0.9
1,936,805 + pykA b1854 0.78 +2.3
1,936,823 - pykA b1854 0.80 -0.2
1,936,823 - pykA b1854 0.80 +0.3
1,937,235 - +0.5
1,937,235 - -2.2
1,937,580 - lpxM b1855 0.34 -0.9
1,937,674 + lpxM b1855 0.44 -0.2
1,937,696 + lpxM b1855 0.46 -2.3
1,937,696 + lpxM b1855 0.46 -1.3
1,937,738 - lpxM b1855 0.51 +0.6
1,937,742 + lpxM b1855 0.51 -0.9
1,937,844 + lpxM b1855 0.62 -1.4
1,937,989 + lpxM b1855 0.76 +0.2
1,937,997 + lpxM b1855 0.77 +0.3
1,938,027 - lpxM b1855 0.80 +0.8

Or see this region's nucleotide sequence