Strain Fitness in Escherichia coli BW25113 around b1264

Experiment: L-Fucose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrpD and trpE overlap by 1 nucleotidestrpE and trpL are separated by 91 nucleotidestrpL and yciV are separated by 137 nucleotides b1263: trpD - bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI), at 1,317,813 to 1,319,408 trpD b1264: trpE - anthranilate synthase component I (NCBI), at 1,319,408 to 1,320,970 trpE b1265: trpL - trp operon leader peptide (NCBI), at 1,321,062 to 1,321,106 trpL b1266: yciV - hypothetical protein (NCBI), at 1,321,244 to 1,322,125 yciV Position (kb) 1319 1320 1321Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1319.016 kb on - strand, within trpDat 1319.138 kb on - strand, within trpDat 1319.228 kb on + strand, within trpDat 1319.372 kb on - strandat 1319.455 kb on - strandat 1319.714 kb on + strand, within trpEat 1319.820 kb on + strand, within trpEat 1320.027 kb on + strand, within trpEat 1320.044 kb on + strand, within trpEat 1320.100 kb on - strand, within trpEat 1320.119 kb on - strand, within trpEat 1320.125 kb on + strand, within trpEat 1320.125 kb on + strand, within trpEat 1320.275 kb on - strand, within trpEat 1320.275 kb on - strand, within trpEat 1320.672 kb on - strand, within trpEat 1320.809 kb on - strand, within trpEat 1320.890 kb on - strandat 1321.053 kb on - strandat 1321.053 kb on - strandat 1321.257 kb on + strandat 1321.332 kb on + strandat 1321.332 kb on + strandat 1321.378 kb on + strand, within yciVat 1321.381 kb on + strand, within yciVat 1321.537 kb on - strand, within yciVat 1321.537 kb on - strand, within yciVat 1321.697 kb on + strand, within yciVat 1321.935 kb on - strand, within yciV

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Fucose (C)
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1,319,016 - trpD b1263 0.75 -2.4
1,319,138 - trpD b1263 0.83 -3.6
1,319,228 + trpD b1263 0.89 -1.2
1,319,372 - -2.5
1,319,455 - -0.7
1,319,714 + trpE b1264 0.20 -2.6
1,319,820 + trpE b1264 0.26 -4.3
1,320,027 + trpE b1264 0.40 -2.4
1,320,044 + trpE b1264 0.41 -3.9
1,320,100 - trpE b1264 0.44 -2.4
1,320,119 - trpE b1264 0.45 -0.5
1,320,125 + trpE b1264 0.46 -1.7
1,320,125 + trpE b1264 0.46 -2.6
1,320,275 - trpE b1264 0.55 -2.0
1,320,275 - trpE b1264 0.55 -1.4
1,320,672 - trpE b1264 0.81 +0.3
1,320,809 - trpE b1264 0.90 +0.0
1,320,890 - -1.2
1,321,053 - -0.7
1,321,053 - +0.3
1,321,257 + +0.3
1,321,332 + +0.9
1,321,332 + +0.2
1,321,378 + yciV b1266 0.15 -0.4
1,321,381 + yciV b1266 0.16 +0.8
1,321,537 - yciV b1266 0.33 +0.0
1,321,537 - yciV b1266 0.33 -1.0
1,321,697 + yciV b1266 0.51 -0.1
1,321,935 - yciV b1266 0.78 +0.1

Or see this region's nucleotide sequence