Strain Fitness in Escherichia coli BW25113 around b1262

Experiment: L-Fucose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrpB and trpC are separated by 11 nucleotidestrpC and trpD are separated by 3 nucleotides b1261: trpB - tryptophan synthase subunit beta (NCBI), at 1,315,246 to 1,316,439 trpB b1262: trpC - N-(5-phosphoribosyl)anthranilate isomerase and indole-3-glycerolphosphate synthetase (VIMSS), at 1,316,451 to 1,317,809 trpC b1263: trpD - bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI), at 1,317,813 to 1,319,408 trpD Position (kb) 1316 1317 1318Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1315.453 kb on + strand, within trpBat 1315.671 kb on + strand, within trpBat 1315.984 kb on - strand, within trpBat 1316.375 kb on - strandat 1316.375 kb on - strandat 1316.600 kb on + strand, within trpCat 1316.692 kb on + strand, within trpCat 1316.692 kb on + strand, within trpCat 1317.035 kb on - strand, within trpCat 1317.035 kb on - strand, within trpCat 1317.113 kb on - strand, within trpCat 1317.144 kb on - strand, within trpCat 1317.153 kb on + strand, within trpCat 1317.181 kb on + strand, within trpCat 1317.305 kb on - strand, within trpCat 1317.389 kb on + strand, within trpCat 1317.389 kb on + strand, within trpCat 1317.643 kb on + strand, within trpCat 1317.684 kb on - strandat 1317.684 kb on - strandat 1317.766 kb on - strandat 1318.316 kb on + strand, within trpDat 1318.316 kb on + strand, within trpD

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Fucose (C)
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1,315,453 + trpB b1261 0.17 -3.3
1,315,671 + trpB b1261 0.36 -3.6
1,315,984 - trpB b1261 0.62 -3.4
1,316,375 - -1.4
1,316,375 - -3.4
1,316,600 + trpC b1262 0.11 -1.7
1,316,692 + trpC b1262 0.18 -2.5
1,316,692 + trpC b1262 0.18 -2.4
1,317,035 - trpC b1262 0.43 +1.1
1,317,035 - trpC b1262 0.43 -1.7
1,317,113 - trpC b1262 0.49 -1.0
1,317,144 - trpC b1262 0.51 -0.7
1,317,153 + trpC b1262 0.52 -2.8
1,317,181 + trpC b1262 0.54 -4.3
1,317,305 - trpC b1262 0.63 -0.2
1,317,389 + trpC b1262 0.69 -2.1
1,317,389 + trpC b1262 0.69 -3.2
1,317,643 + trpC b1262 0.88 -4.6
1,317,684 - -1.2
1,317,684 - -0.4
1,317,766 - -2.7
1,318,316 + trpD b1263 0.32 -2.7
1,318,316 + trpD b1263 0.32 -2.7

Or see this region's nucleotide sequence