Strain Fitness in Escherichia coli BW25113 around b1129

Experiment: L-Fucose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycfD and phoQ are separated by 75 nucleotidesphoQ and phoP overlap by 1 nucleotidesphoP and purB are separated by 168 nucleotides b1128: ycfD - orf, hypothetical protein (VIMSS), at 1,186,342 to 1,187,463 ycfD b1129: phoQ - sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI), at 1,187,539 to 1,188,999 phoQ b1130: phoP - DNA-binding response regulator in two-component regulatory system with PhoQ (NCBI), at 1,188,999 to 1,189,670 phoP b1131: purB - adenylosuccinate lyase (NCBI), at 1,189,839 to 1,191,209 purB Position (kb) 1187 1188 1189Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1186.778 kb on + strand, within ycfDat 1186.778 kb on + strand, within ycfDat 1186.805 kb on - strand, within ycfDat 1186.876 kb on - strand, within ycfDat 1186.942 kb on - strand, within ycfDat 1186.950 kb on + strand, within ycfDat 1186.950 kb on + strand, within ycfDat 1187.202 kb on + strand, within ycfDat 1187.292 kb on + strand, within ycfDat 1187.292 kb on + strand, within ycfDat 1187.349 kb on + strand, within ycfDat 1187.349 kb on + strand, within ycfDat 1187.446 kb on + strandat 1187.521 kb on + strandat 1187.672 kb on - strandat 1187.672 kb on - strandat 1187.674 kb on - strandat 1187.674 kb on - strandat 1187.685 kb on + strand, within phoQat 1187.775 kb on - strand, within phoQat 1187.776 kb on - strand, within phoQat 1187.776 kb on - strand, within phoQat 1187.779 kb on + strand, within phoQat 1187.844 kb on - strand, within phoQat 1187.955 kb on + strand, within phoQat 1187.973 kb on - strand, within phoQat 1188.100 kb on - strand, within phoQat 1188.191 kb on + strand, within phoQat 1188.246 kb on + strand, within phoQat 1188.320 kb on + strand, within phoQat 1188.342 kb on + strand, within phoQat 1188.342 kb on + strand, within phoQat 1188.435 kb on - strand, within phoQat 1188.435 kb on - strand, within phoQat 1188.461 kb on + strand, within phoQat 1188.564 kb on + strand, within phoQat 1188.760 kb on - strand, within phoQat 1188.760 kb on - strand, within phoQat 1188.911 kb on + strandat 1188.924 kb on + strandat 1189.023 kb on - strandat 1189.104 kb on + strand, within phoPat 1189.121 kb on + strand, within phoPat 1189.135 kb on - strand, within phoPat 1189.135 kb on - strand, within phoPat 1189.137 kb on + strand, within phoPat 1189.137 kb on + strand, within phoPat 1189.137 kb on + strand, within phoPat 1189.320 kb on - strand, within phoPat 1189.320 kb on - strand, within phoPat 1189.330 kb on - strand, within phoPat 1189.375 kb on - strand, within phoPat 1189.375 kb on - strand, within phoPat 1189.395 kb on + strand, within phoPat 1189.403 kb on - strand, within phoPat 1189.483 kb on + strand, within phoPat 1189.587 kb on + strand, within phoPat 1189.587 kb on + strand, within phoPat 1189.590 kb on + strand, within phoPat 1189.614 kb on - strandat 1189.713 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Fucose (C)
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1,186,778 + ycfD b1128 0.39 -1.8
1,186,778 + ycfD b1128 0.39 -0.1
1,186,805 - ycfD b1128 0.41 +0.0
1,186,876 - ycfD b1128 0.48 -0.4
1,186,942 - ycfD b1128 0.53 +0.6
1,186,950 + ycfD b1128 0.54 +0.2
1,186,950 + ycfD b1128 0.54 +0.2
1,187,202 + ycfD b1128 0.77 -0.8
1,187,292 + ycfD b1128 0.85 -0.0
1,187,292 + ycfD b1128 0.85 +0.8
1,187,349 + ycfD b1128 0.90 +0.4
1,187,349 + ycfD b1128 0.90 +0.6
1,187,446 + +0.4
1,187,521 + +0.3
1,187,672 - -2.4
1,187,672 - -1.4
1,187,674 - -2.7
1,187,674 - -3.3
1,187,685 + phoQ b1129 0.10 -2.3
1,187,775 - phoQ b1129 0.16 -2.8
1,187,776 - phoQ b1129 0.16 -3.2
1,187,776 - phoQ b1129 0.16 -3.6
1,187,779 + phoQ b1129 0.16 -2.2
1,187,844 - phoQ b1129 0.21 -1.7
1,187,955 + phoQ b1129 0.28 -3.4
1,187,973 - phoQ b1129 0.30 -3.2
1,188,100 - phoQ b1129 0.38 -3.1
1,188,191 + phoQ b1129 0.45 -3.3
1,188,246 + phoQ b1129 0.48 -1.5
1,188,320 + phoQ b1129 0.53 -3.0
1,188,342 + phoQ b1129 0.55 -3.0
1,188,342 + phoQ b1129 0.55 -3.5
1,188,435 - phoQ b1129 0.61 -2.6
1,188,435 - phoQ b1129 0.61 -2.8
1,188,461 + phoQ b1129 0.63 -3.4
1,188,564 + phoQ b1129 0.70 -2.8
1,188,760 - phoQ b1129 0.84 -1.8
1,188,760 - phoQ b1129 0.84 -2.7
1,188,911 + -2.0
1,188,924 + -2.5
1,189,023 - -3.8
1,189,104 + phoP b1130 0.16 -2.6
1,189,121 + phoP b1130 0.18 -3.2
1,189,135 - phoP b1130 0.20 -2.3
1,189,135 - phoP b1130 0.20 -2.4
1,189,137 + phoP b1130 0.21 -2.9
1,189,137 + phoP b1130 0.21 -3.6
1,189,137 + phoP b1130 0.21 -1.5
1,189,320 - phoP b1130 0.48 -3.8
1,189,320 - phoP b1130 0.48 -3.6
1,189,330 - phoP b1130 0.49 -3.3
1,189,375 - phoP b1130 0.56 -1.4
1,189,375 - phoP b1130 0.56 -2.0
1,189,395 + phoP b1130 0.59 -1.7
1,189,403 - phoP b1130 0.60 -2.2
1,189,483 + phoP b1130 0.72 -3.2
1,189,587 + phoP b1130 0.88 -1.5
1,189,587 + phoP b1130 0.88 -2.6
1,189,590 + phoP b1130 0.88 -4.0
1,189,614 - -1.7
1,189,713 + -1.9

Or see this region's nucleotide sequence