Strain Fitness in Escherichia coli BW25113 around b0032

Experiment: L-Fucose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdapB and carA are separated by 455 nucleotidescarA and carB are separated by 17 nucleotides b0031: dapB - dihydrodipicolinate reductase (NCBI), at 28,374 to 29,195 dapB b0032: carA - carbamoyl-phosphate synthase small subunit (NCBI), at 29,651 to 30,799 carA b0033: carB - carbamoyl-phosphate synthase large subunit (NCBI), at 30,817 to 34,038 carB Position (kb) 29 30 31Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 29.587 kb on + strandat 29.661 kb on - strandat 29.661 kb on - strandat 29.841 kb on + strand, within carAat 29.872 kb on + strand, within carAat 29.931 kb on + strand, within carAat 29.931 kb on + strand, within carAat 30.037 kb on - strand, within carAat 30.104 kb on + strand, within carAat 30.154 kb on + strand, within carAat 30.156 kb on - strand, within carAat 30.156 kb on - strand, within carAat 30.320 kb on + strand, within carAat 30.364 kb on + strand, within carAat 30.364 kb on + strand, within carAat 30.588 kb on + strand, within carAat 30.768 kb on + strandat 30.770 kb on + strandat 30.773 kb on + strandat 30.786 kb on - strandat 30.858 kb on - strandat 30.868 kb on + strandat 30.868 kb on + strandat 30.889 kb on - strandat 30.917 kb on + strandat 30.917 kb on + strandat 31.041 kb on - strandat 31.099 kb on + strandat 31.099 kb on + strandat 31.099 kb on + strandat 31.099 kb on + strandat 31.101 kb on + strandat 31.107 kb on - strandat 31.144 kb on - strand, within carBat 31.151 kb on + strand, within carBat 31.157 kb on - strand, within carBat 31.157 kb on - strand, within carBat 31.161 kb on + strand, within carBat 31.188 kb on - strand, within carBat 31.190 kb on + strand, within carBat 31.242 kb on - strand, within carBat 31.259 kb on - strand, within carBat 31.327 kb on + strand, within carBat 31.327 kb on + strand, within carBat 31.341 kb on + strand, within carBat 31.359 kb on + strand, within carBat 31.359 kb on + strand, within carBat 31.360 kb on + strand, within carBat 31.425 kb on - strand, within carBat 31.466 kb on + strand, within carBat 31.643 kb on + strand, within carBat 31.726 kb on - strand, within carBat 31.748 kb on + strand, within carB

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Fucose (C)
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29,587 + -0.3
29,661 - -2.5
29,661 - -2.0
29,841 + carA b0032 0.17 -4.2
29,872 + carA b0032 0.19 -1.5
29,931 + carA b0032 0.24 -3.3
29,931 + carA b0032 0.24 -1.3
30,037 - carA b0032 0.34 -3.1
30,104 + carA b0032 0.39 -2.8
30,154 + carA b0032 0.44 -1.5
30,156 - carA b0032 0.44 -1.2
30,156 - carA b0032 0.44 -1.1
30,320 + carA b0032 0.58 -3.0
30,364 + carA b0032 0.62 -1.8
30,364 + carA b0032 0.62 -1.8
30,588 + carA b0032 0.82 -4.2
30,768 + -0.5
30,770 + -0.7
30,773 + -0.4
30,786 - -2.3
30,858 - -2.5
30,868 + -1.9
30,868 + -1.9
30,889 - -2.3
30,917 + -0.5
30,917 + -0.8
31,041 - -0.5
31,099 + -0.3
31,099 + -1.7
31,099 + -1.9
31,099 + -0.9
31,101 + -2.7
31,107 - -3.0
31,144 - carB b0033 0.10 -1.6
31,151 + carB b0033 0.10 -2.2
31,157 - carB b0033 0.11 -3.8
31,157 - carB b0033 0.11 -3.3
31,161 + carB b0033 0.11 -1.9
31,188 - carB b0033 0.12 -2.5
31,190 + carB b0033 0.12 -2.8
31,242 - carB b0033 0.13 -1.6
31,259 - carB b0033 0.14 -1.6
31,327 + carB b0033 0.16 -3.4
31,327 + carB b0033 0.16 -2.3
31,341 + carB b0033 0.16 -2.8
31,359 + carB b0033 0.17 -3.0
31,359 + carB b0033 0.17 -4.6
31,360 + carB b0033 0.17 -1.6
31,425 - carB b0033 0.19 -2.7
31,466 + carB b0033 0.20 -0.9
31,643 + carB b0033 0.26 -2.4
31,726 - carB b0033 0.28 -0.9
31,748 + carB b0033 0.29 -0.3

Or see this region's nucleotide sequence