Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_321 and PfGW456L13_322 are separated by 150 nucleotides PfGW456L13_322 and PfGW456L13_323 overlap by 4 nucleotides PfGW456L13_323 and PfGW456L13_324 are separated by 70 nucleotides
PfGW456L13_321 - Histidine ammonia-lyase (EC 4.3.1.3), at 375,421 to 376,944
_321
PfGW456L13_322 - Histidine ABC transporter, ATP-binding protein (TC 3.A.1), at 377,095 to 377,925
_322
PfGW456L13_323 - Histidine ABC transporter, permease protein (TC 3.A.1), at 377,922 to 378,773
_323
PfGW456L13_324 - Histidine ABC transporter, histidine-binding protein (TC 3.A.1), at 378,844 to 379,812
_324
Position (kb)
377
378 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 376.451 kb on + strand, within PfGW456L13_321 at 376.452 kb on - strand, within PfGW456L13_321 at 376.613 kb on + strand, within PfGW456L13_321 at 376.613 kb on + strand, within PfGW456L13_321 at 376.666 kb on + strand, within PfGW456L13_321 at 376.959 kb on + strand at 376.960 kb on - strand at 376.960 kb on - strand at 376.960 kb on - strand at 376.972 kb on - strand at 377.096 kb on + strand at 377.096 kb on + strand at 377.097 kb on - strand at 377.135 kb on + strand at 377.135 kb on + strand at 377.135 kb on + strand at 377.135 kb on + strand at 377.135 kb on + strand at 377.136 kb on - strand at 377.136 kb on - strand at 377.136 kb on - strand at 377.136 kb on - strand at 377.433 kb on - strand, within PfGW456L13_322 at 377.433 kb on - strand, within PfGW456L13_322 at 377.631 kb on - strand, within PfGW456L13_322 at 377.631 kb on - strand, within PfGW456L13_322 at 377.953 kb on + strand at 377.953 kb on + strand at 377.953 kb on + strand at 377.954 kb on - strand at 377.954 kb on - strand at 377.954 kb on - strand at 377.963 kb on + strand at 377.963 kb on + strand at 377.964 kb on - strand at 377.964 kb on - strand at 378.046 kb on - strand, within PfGW456L13_323 at 378.283 kb on + strand, within PfGW456L13_323 at 378.284 kb on - strand, within PfGW456L13_323 at 378.284 kb on - strand, within PfGW456L13_323 at 378.349 kb on + strand, within PfGW456L13_323 at 378.350 kb on - strand, within PfGW456L13_323 at 378.589 kb on - strand, within PfGW456L13_323 at 378.755 kb on + strand at 378.773 kb on + strand at 378.773 kb on + strand at 378.773 kb on + strand at 378.773 kb on + strand at 378.773 kb on + strand at 378.774 kb on - strand at 378.774 kb on - strand at 378.774 kb on - strand at 378.774 kb on - strand at 378.774 kb on - strand at 378.845 kb on + strand at 378.845 kb on + strand at 378.845 kb on + strand at 378.846 kb on - strand at 378.846 kb on - strand at 378.846 kb on - strand at 378.846 kb on - strand at 378.846 kb on - strand at 378.846 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 376,451 + PfGW456L13_321 0.68 -2.4 376,452 - PfGW456L13_321 0.68 +0.2 376,613 + PfGW456L13_321 0.78 -4.6 376,613 + PfGW456L13_321 0.78 -4.1 376,666 + PfGW456L13_321 0.82 -3.1 376,959 + -1.7 376,960 - -3.2 376,960 - -1.6 376,960 - -2.3 376,972 - -1.1 377,096 + -1.9 377,096 + -2.3 377,097 - +1.4 377,135 + -4.0 377,135 + -3.8 377,135 + -3.1 377,135 + -2.7 377,135 + -2.8 377,136 - +0.9 377,136 - +1.2 377,136 - +1.6 377,136 - +0.9 377,433 - PfGW456L13_322 0.41 +0.3 377,433 - PfGW456L13_322 0.41 -0.0 377,631 - PfGW456L13_322 0.65 -1.8 377,631 - PfGW456L13_322 0.65 -0.7 377,953 + -3.0 377,953 + -4.7 377,953 + -4.0 377,954 - -1.1 377,954 - +0.9 377,954 - +0.4 377,963 + -3.1 377,963 + -2.4 377,964 - +1.7 377,964 - -1.7 378,046 - PfGW456L13_323 0.15 +0.3 378,283 + PfGW456L13_323 0.42 -3.2 378,284 - PfGW456L13_323 0.42 +1.1 378,284 - PfGW456L13_323 0.42 +0.7 378,349 + PfGW456L13_323 0.50 -3.0 378,350 - PfGW456L13_323 0.50 +1.8 378,589 - PfGW456L13_323 0.78 -1.1 378,755 + -4.6 378,773 + -4.0 378,773 + -3.1 378,773 + -4.4 378,773 + -1.5 378,773 + -2.5 378,774 - -3.6 378,774 - -2.0 378,774 - -1.4 378,774 - -2.2 378,774 - -2.7 378,845 + -3.3 378,845 + -4.0 378,845 + -2.2 378,846 - +0.8 378,846 - +1.5 378,846 - +1.5 378,846 - +1.3 378,846 - +1.7 378,846 - -0.4
Or see this region's nucleotide sequence