Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS03830 and PFLU_RS03835 are separated by 193 nucleotides PFLU_RS03835 and PFLU_RS03840 overlap by 4 nucleotides PFLU_RS03840 and PFLU_RS03845 are separated by 7 nucleotides PFLU_RS03845 and PFLU_RS03850 are separated by 22 nucleotides
PFLU_RS03830: PFLU_RS03830 - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 875,032 to 875,613
_RS03830
PFLU_RS03835: PFLU_RS03835 - type IV pilus modification protein PilV, at 875,807 to 876,292
_RS03835
PFLU_RS03840: PFLU_RS03840 - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 876,289 to 876,996
_RS03840
PFLU_RS03845: PFLU_RS03845 - pilus assembly protein PilX, at 877,004 to 877,534
_RS03845
PFLU_RS03850: PFLU_RS03850 - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 877,557 to 877,958
_RS03850
Position (kb) 876
877 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 875.468 kb on - strand, within PFLU_RS03830 at 875.498 kb on + strand, within PFLU_RS03830 at 875.498 kb on + strand, within PFLU_RS03830 at 875.498 kb on + strand, within PFLU_RS03830 at 875.499 kb on - strand, within PFLU_RS03830 at 875.646 kb on + strand at 875.646 kb on + strand at 875.646 kb on + strand at 875.647 kb on - strand at 875.650 kb on + strand at 875.650 kb on + strand at 875.650 kb on + strand at 875.650 kb on + strand at 875.651 kb on - strand at 875.651 kb on - strand at 875.651 kb on - strand at 875.651 kb on - strand at 875.651 kb on - strand at 875.651 kb on - strand at 875.652 kb on + strand at 875.652 kb on + strand at 875.653 kb on - strand at 875.653 kb on - strand at 875.686 kb on + strand at 875.686 kb on + strand at 875.686 kb on + strand at 875.686 kb on + strand at 875.687 kb on - strand at 875.687 kb on - strand at 875.697 kb on + strand at 875.698 kb on - strand at 875.698 kb on - strand at 875.698 kb on - strand at 875.741 kb on + strand at 875.805 kb on + strand at 875.893 kb on - strand, within PFLU_RS03835 at 875.951 kb on + strand, within PFLU_RS03835 at 875.951 kb on + strand, within PFLU_RS03835 at 875.951 kb on + strand, within PFLU_RS03835 at 875.951 kb on + strand, within PFLU_RS03835 at 875.952 kb on - strand, within PFLU_RS03835 at 875.952 kb on - strand, within PFLU_RS03835 at 875.952 kb on - strand, within PFLU_RS03835 at 875.952 kb on - strand, within PFLU_RS03835 at 875.997 kb on - strand, within PFLU_RS03835 at 876.038 kb on + strand, within PFLU_RS03835 at 876.038 kb on + strand, within PFLU_RS03835 at 876.038 kb on + strand, within PFLU_RS03835 at 876.039 kb on - strand, within PFLU_RS03835 at 876.039 kb on - strand, within PFLU_RS03835 at 876.039 kb on - strand, within PFLU_RS03835 at 876.039 kb on - strand, within PFLU_RS03835 at 876.125 kb on - strand, within PFLU_RS03835 at 876.125 kb on - strand, within PFLU_RS03835 at 876.125 kb on - strand, within PFLU_RS03835 at 876.264 kb on + strand at 876.265 kb on - strand at 876.456 kb on + strand, within PFLU_RS03840 at 876.457 kb on - strand, within PFLU_RS03840 at 876.513 kb on + strand, within PFLU_RS03840 at 876.618 kb on + strand, within PFLU_RS03840 at 876.618 kb on + strand, within PFLU_RS03840 at 876.619 kb on - strand, within PFLU_RS03840 at 876.667 kb on + strand, within PFLU_RS03840 at 876.667 kb on + strand, within PFLU_RS03840 at 876.667 kb on + strand, within PFLU_RS03840 at 876.668 kb on - strand, within PFLU_RS03840 at 876.668 kb on - strand, within PFLU_RS03840 at 876.668 kb on - strand, within PFLU_RS03840 at 876.668 kb on - strand, within PFLU_RS03840 at 876.730 kb on + strand, within PFLU_RS03840 at 876.730 kb on + strand, within PFLU_RS03840 at 876.730 kb on + strand, within PFLU_RS03840 at 876.730 kb on + strand, within PFLU_RS03840 at 876.730 kb on + strand, within PFLU_RS03840 at 876.731 kb on - strand, within PFLU_RS03840 at 876.731 kb on - strand, within PFLU_RS03840 at 876.731 kb on - strand, within PFLU_RS03840 at 876.731 kb on - strand, within PFLU_RS03840 at 876.731 kb on - strand, within PFLU_RS03840 at 876.731 kb on - strand, within PFLU_RS03840 at 876.731 kb on - strand, within PFLU_RS03840 at 876.731 kb on - strand, within PFLU_RS03840 at 876.731 kb on - strand, within PFLU_RS03840 at 876.788 kb on + strand, within PFLU_RS03840 at 876.865 kb on + strand, within PFLU_RS03840 at 876.897 kb on - strand, within PFLU_RS03840 at 876.958 kb on + strand at 876.958 kb on + strand at 876.958 kb on + strand at 876.958 kb on + strand at 876.958 kb on + strand at 876.959 kb on - strand at 876.994 kb on + strand at 876.995 kb on - strand at 877.153 kb on + strand, within PFLU_RS03845 at 877.154 kb on - strand, within PFLU_RS03845 at 877.206 kb on + strand, within PFLU_RS03845 at 877.206 kb on + strand, within PFLU_RS03845 at 877.207 kb on - strand, within PFLU_RS03845 at 877.207 kb on - strand, within PFLU_RS03845 at 877.207 kb on - strand, within PFLU_RS03845 at 877.371 kb on + strand, within PFLU_RS03845 at 877.371 kb on + strand, within PFLU_RS03845 at 877.372 kb on - strand, within PFLU_RS03845 at 877.456 kb on - strand, within PFLU_RS03845 at 877.456 kb on - strand, within PFLU_RS03845 at 877.456 kb on - strand, within PFLU_RS03845 at 877.469 kb on + strand, within PFLU_RS03845 at 877.470 kb on - strand, within PFLU_RS03845 at 877.470 kb on - strand, within PFLU_RS03845 at 877.532 kb on + strand at 877.533 kb on - strand at 877.533 kb on - strand at 877.533 kb on - strand at 877.542 kb on + strand at 877.542 kb on + strand at 877.542 kb on + strand at 877.542 kb on + strand at 877.542 kb on + strand at 877.542 kb on + strand at 877.542 kb on + strand at 877.542 kb on + strand at 877.542 kb on + strand at 877.542 kb on + strand at 877.542 kb on + strand at 877.542 kb on + strand at 877.542 kb on + strand at 877.542 kb on + strand at 877.542 kb on + strand at 877.542 kb on + strand at 877.543 kb on - strand at 877.543 kb on - strand at 877.543 kb on - strand at 877.543 kb on - strand at 877.543 kb on - strand at 877.543 kb on - strand at 877.543 kb on - strand at 877.543 kb on - strand at 877.543 kb on - strand at 877.543 kb on - strand at 877.543 kb on - strand at 877.543 kb on - strand at 877.543 kb on - strand at 877.543 kb on - strand at 877.543 kb on - strand at 877.543 kb on - strand at 877.580 kb on + strand at 877.581 kb on - strand at 877.594 kb on + strand at 877.594 kb on + strand at 877.595 kb on - strand at 877.595 kb on - strand at 877.638 kb on + strand, within PFLU_RS03850 at 877.638 kb on + strand, within PFLU_RS03850 at 877.639 kb on - strand, within PFLU_RS03850 at 877.647 kb on + strand, within PFLU_RS03850 at 877.647 kb on + strand, within PFLU_RS03850 at 877.693 kb on + strand, within PFLU_RS03850 at 877.693 kb on + strand, within PFLU_RS03850 at 877.693 kb on + strand, within PFLU_RS03850 at 877.748 kb on + strand, within PFLU_RS03850 at 877.748 kb on + strand, within PFLU_RS03850 at 877.749 kb on - strand, within PFLU_RS03850 at 877.787 kb on + strand, within PFLU_RS03850 at 877.787 kb on + strand, within PFLU_RS03850 at 877.787 kb on + strand, within PFLU_RS03850 at 877.787 kb on + strand, within PFLU_RS03850 at 877.787 kb on + strand, within PFLU_RS03850 at 877.787 kb on + strand, within PFLU_RS03850 at 877.787 kb on + strand, within PFLU_RS03850 at 877.787 kb on + strand, within PFLU_RS03850 at 877.787 kb on + strand, within PFLU_RS03850 at 877.787 kb on + strand, within PFLU_RS03850 at 877.787 kb on + strand, within PFLU_RS03850 at 877.788 kb on - strand, within PFLU_RS03850 at 877.879 kb on - strand, within PFLU_RS03850
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 875,468 - PFLU_RS03830 0.75 +0.3 875,498 + PFLU_RS03830 0.80 +1.3 875,498 + PFLU_RS03830 0.80 -0.6 875,498 + PFLU_RS03830 0.80 -0.4 875,499 - PFLU_RS03830 0.80 -0.5 875,646 + +0.3 875,646 + -0.3 875,646 + +0.1 875,647 - +1.2 875,650 + +0.3 875,650 + +0.2 875,650 + -1.4 875,650 + +0.5 875,651 - +0.7 875,651 - -0.7 875,651 - +0.7 875,651 - -1.0 875,651 - -0.1 875,651 - +0.1 875,652 + +0.4 875,652 + +0.4 875,653 - -0.8 875,653 - -1.6 875,686 + +0.2 875,686 + -0.6 875,686 + -2.0 875,686 + -2.4 875,687 - +1.2 875,687 - -0.6 875,697 + +0.2 875,698 - -0.9 875,698 - +1.3 875,698 - -0.4 875,741 + -0.6 875,805 + +0.5 875,893 - PFLU_RS03835 0.18 +0.6 875,951 + PFLU_RS03835 0.30 +0.4 875,951 + PFLU_RS03835 0.30 +1.1 875,951 + PFLU_RS03835 0.30 +0.7 875,951 + PFLU_RS03835 0.30 +0.1 875,952 - PFLU_RS03835 0.30 +0.6 875,952 - PFLU_RS03835 0.30 -0.6 875,952 - PFLU_RS03835 0.30 -0.4 875,952 - PFLU_RS03835 0.30 -0.6 875,997 - PFLU_RS03835 0.39 -0.1 876,038 + PFLU_RS03835 0.48 +0.3 876,038 + PFLU_RS03835 0.48 +0.6 876,038 + PFLU_RS03835 0.48 +0.3 876,039 - PFLU_RS03835 0.48 +0.1 876,039 - PFLU_RS03835 0.48 -1.3 876,039 - PFLU_RS03835 0.48 +1.1 876,039 - PFLU_RS03835 0.48 -0.6 876,125 - PFLU_RS03835 0.65 +0.9 876,125 - PFLU_RS03835 0.65 +0.7 876,125 - PFLU_RS03835 0.65 -0.1 876,264 + +0.5 876,265 - +1.4 876,456 + PFLU_RS03840 0.24 +0.4 876,457 - PFLU_RS03840 0.24 +1.5 876,513 + PFLU_RS03840 0.32 -0.0 876,618 + PFLU_RS03840 0.46 +0.6 876,618 + PFLU_RS03840 0.46 +1.0 876,619 - PFLU_RS03840 0.47 +0.8 876,667 + PFLU_RS03840 0.53 -0.5 876,667 + PFLU_RS03840 0.53 -0.6 876,667 + PFLU_RS03840 0.53 -0.4 876,668 - PFLU_RS03840 0.54 +0.1 876,668 - PFLU_RS03840 0.54 +1.2 876,668 - PFLU_RS03840 0.54 +0.8 876,668 - PFLU_RS03840 0.54 -1.5 876,730 + PFLU_RS03840 0.62 -0.8 876,730 + PFLU_RS03840 0.62 +0.6 876,730 + PFLU_RS03840 0.62 +0.3 876,730 + PFLU_RS03840 0.62 +0.6 876,730 + PFLU_RS03840 0.62 +0.0 876,731 - PFLU_RS03840 0.62 +0.3 876,731 - PFLU_RS03840 0.62 +1.6 876,731 - PFLU_RS03840 0.62 -0.2 876,731 - PFLU_RS03840 0.62 +0.6 876,731 - PFLU_RS03840 0.62 +0.5 876,731 - PFLU_RS03840 0.62 -0.6 876,731 - PFLU_RS03840 0.62 +0.5 876,731 - PFLU_RS03840 0.62 -0.0 876,731 - PFLU_RS03840 0.62 +2.5 876,788 + PFLU_RS03840 0.70 +1.0 876,865 + PFLU_RS03840 0.81 -0.5 876,897 - PFLU_RS03840 0.86 -0.8 876,958 + -0.3 876,958 + +0.1 876,958 + +0.4 876,958 + -0.1 876,958 + +1.6 876,959 - +0.1 876,994 + +0.6 876,995 - -1.1 877,153 + PFLU_RS03845 0.28 -0.2 877,154 - PFLU_RS03845 0.28 +1.2 877,206 + PFLU_RS03845 0.38 +1.1 877,206 + PFLU_RS03845 0.38 -0.8 877,207 - PFLU_RS03845 0.38 +0.6 877,207 - PFLU_RS03845 0.38 +0.8 877,207 - PFLU_RS03845 0.38 -0.7 877,371 + PFLU_RS03845 0.69 +1.8 877,371 + PFLU_RS03845 0.69 +1.0 877,372 - PFLU_RS03845 0.69 -0.8 877,456 - PFLU_RS03845 0.85 -0.8 877,456 - PFLU_RS03845 0.85 -1.2 877,456 - PFLU_RS03845 0.85 -1.0 877,469 + PFLU_RS03845 0.88 -0.4 877,470 - PFLU_RS03845 0.88 +0.4 877,470 - PFLU_RS03845 0.88 -1.0 877,532 + -0.7 877,533 - -0.2 877,533 - +1.0 877,533 - -0.2 877,542 + +0.4 877,542 + +0.6 877,542 + -0.5 877,542 + +0.3 877,542 + +0.3 877,542 + -1.2 877,542 + -0.2 877,542 + +0.8 877,542 + -0.9 877,542 + +0.6 877,542 + +0.6 877,542 + -1.1 877,542 + -1.0 877,542 + -0.3 877,542 + +0.0 877,542 + +0.2 877,543 - -0.5 877,543 - -0.3 877,543 - +0.8 877,543 - -0.2 877,543 - +0.7 877,543 - -0.9 877,543 - +2.8 877,543 - +0.5 877,543 - -0.6 877,543 - +1.7 877,543 - -0.2 877,543 - +2.1 877,543 - +1.8 877,543 - -0.7 877,543 - -0.0 877,543 - -0.0 877,580 + +0.1 877,581 - +0.0 877,594 + +1.0 877,594 + -0.4 877,595 - -0.8 877,595 - +0.4 877,638 + PFLU_RS03850 0.20 +0.5 877,638 + PFLU_RS03850 0.20 -0.0 877,639 - PFLU_RS03850 0.20 +0.1 877,647 + PFLU_RS03850 0.22 +1.4 877,647 + PFLU_RS03850 0.22 +2.8 877,693 + PFLU_RS03850 0.34 -0.9 877,693 + PFLU_RS03850 0.34 -0.5 877,693 + PFLU_RS03850 0.34 +1.1 877,748 + PFLU_RS03850 0.48 -0.2 877,748 + PFLU_RS03850 0.48 -0.6 877,749 - PFLU_RS03850 0.48 -0.4 877,787 + PFLU_RS03850 0.57 -0.1 877,787 + PFLU_RS03850 0.57 -0.1 877,787 + PFLU_RS03850 0.57 +1.1 877,787 + PFLU_RS03850 0.57 -0.4 877,787 + PFLU_RS03850 0.57 -0.3 877,787 + PFLU_RS03850 0.57 +1.8 877,787 + PFLU_RS03850 0.57 -0.5 877,787 + PFLU_RS03850 0.57 -0.5 877,787 + PFLU_RS03850 0.57 -0.7 877,787 + PFLU_RS03850 0.57 -0.8 877,787 + PFLU_RS03850 0.57 -1.3 877,788 - PFLU_RS03850 0.57 -0.1 877,879 - PFLU_RS03850 0.80 +0.5
Or see this region's nucleotide sequence