Experiment: KB with Berberine 15.6 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4635 and Psyr_4636 overlap by 10 nucleotides Psyr_4636 and Psyr_4637 are separated by 65 nucleotides Psyr_4637 and Psyr_4638 are separated by 23 nucleotides
Psyr_4635: Psyr_4635 - 7, 8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase, HPPK, at 5,496,742 to 5,497,266
_4635
Psyr_4636: Psyr_4636 - Dihydroneopterin aldolase family:Dihydroneopterin aldolase, at 5,497,257 to 5,497,610
_4636
Psyr_4637: Psyr_4637 - acyl-phosphate glycerol-3-phosphate acyltransferase, at 5,497,676 to 5,498,254
_4637
Psyr_4638: Psyr_4638 - O-sialoglycoprotein endopeptidase, at 5,498,278 to 5,499,303
_4638
Position (kb) 5497
5498
5499 Strain fitness (log2 ratio) -2
-1
0
1 at 5496.688 kb on + strand at 5496.692 kb on + strand at 5496.692 kb on + strand at 5496.692 kb on + strand at 5496.692 kb on + strand at 5496.692 kb on + strand at 5496.692 kb on + strand at 5496.692 kb on + strand at 5496.692 kb on + strand at 5496.693 kb on - strand at 5496.693 kb on - strand at 5496.748 kb on - strand at 5496.905 kb on - strand, within Psyr_4635 at 5496.905 kb on - strand, within Psyr_4635 at 5497.652 kb on - strand at 5497.970 kb on + strand, within Psyr_4637 at 5497.970 kb on + strand, within Psyr_4637 at 5497.971 kb on - strand, within Psyr_4637 at 5497.971 kb on - strand, within Psyr_4637 at 5497.971 kb on - strand, within Psyr_4637 at 5498.055 kb on + strand, within Psyr_4637 at 5498.217 kb on + strand at 5498.218 kb on - strand at 5498.218 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 15.6 ug/ml remove 5,496,688 + -0.3 5,496,692 + -0.5 5,496,692 + -0.4 5,496,692 + +0.4 5,496,692 + -1.5 5,496,692 + -1.1 5,496,692 + +0.8 5,496,692 + -0.6 5,496,692 + +0.2 5,496,693 - -0.3 5,496,693 - -0.1 5,496,748 - +0.2 5,496,905 - Psyr_4635 0.31 +0.8 5,496,905 - Psyr_4635 0.31 -0.3 5,497,652 - -0.3 5,497,970 + Psyr_4637 0.51 -0.5 5,497,970 + Psyr_4637 0.51 -0.5 5,497,971 - Psyr_4637 0.51 +0.9 5,497,971 - Psyr_4637 0.51 -1.8 5,497,971 - Psyr_4637 0.51 -1.2 5,498,055 + Psyr_4637 0.65 +1.0 5,498,217 + +1.0 5,498,218 - +0.7 5,498,218 - -0.4
Or see this region's nucleotide sequence