Experiment: KB with Berberine 15.6 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3891 and Psyr_3892 are separated by 383 nucleotides Psyr_3892 and Psyr_3893 are separated by 120 nucleotides
Psyr_3891: Psyr_3891 - hypothetical protein, at 4,632,562 to 4,633,089
_3891
Psyr_3892: Psyr_3892 - argininosuccinate synthase, at 4,633,473 to 4,634,690
_3892
Psyr_3893: Psyr_3893 - OmpA/MotB, at 4,634,811 to 4,635,713
_3893
Position (kb)
4633
4634
4635 Strain fitness (log2 ratio)
-1
0
1 at 4632.535 kb on - strand at 4632.535 kb on - strand at 4632.558 kb on + strand at 4632.558 kb on + strand at 4632.558 kb on + strand at 4632.559 kb on - strand at 4632.570 kb on + strand at 4632.574 kb on + strand at 4632.575 kb on - strand at 4632.575 kb on - strand at 4632.626 kb on + strand, within Psyr_3891 at 4632.626 kb on + strand, within Psyr_3891 at 4632.627 kb on - strand, within Psyr_3891 at 4632.751 kb on - strand, within Psyr_3891 at 4632.803 kb on + strand, within Psyr_3891 at 4632.804 kb on - strand, within Psyr_3891 at 4632.825 kb on + strand, within Psyr_3891 at 4632.826 kb on - strand, within Psyr_3891 at 4632.858 kb on + strand, within Psyr_3891 at 4632.859 kb on - strand, within Psyr_3891 at 4632.922 kb on - strand, within Psyr_3891 at 4633.025 kb on + strand, within Psyr_3891 at 4633.121 kb on + strand at 4633.122 kb on - strand at 4633.125 kb on + strand at 4633.125 kb on + strand at 4633.126 kb on - strand at 4633.126 kb on - strand at 4633.153 kb on - strand at 4633.183 kb on + strand at 4633.204 kb on - strand at 4633.227 kb on - strand at 4633.227 kb on - strand at 4633.304 kb on - strand at 4633.339 kb on + strand at 4633.339 kb on + strand at 4633.339 kb on + strand at 4633.339 kb on + strand at 4633.339 kb on + strand at 4633.339 kb on + strand at 4633.339 kb on + strand at 4633.339 kb on + strand at 4633.340 kb on - strand at 4633.340 kb on - strand at 4633.340 kb on - strand at 4633.340 kb on - strand at 4633.342 kb on - strand at 4633.342 kb on - strand at 4633.342 kb on - strand at 4633.419 kb on + strand at 4633.419 kb on + strand at 4633.422 kb on + strand at 4633.422 kb on + strand at 4633.422 kb on + strand at 4634.804 kb on - strand at 4634.812 kb on + strand at 4634.812 kb on + strand at 4635.001 kb on + strand, within Psyr_3893 at 4635.001 kb on + strand, within Psyr_3893 at 4635.002 kb on - strand, within Psyr_3893 at 4635.288 kb on + strand, within Psyr_3893 at 4635.289 kb on - strand, within Psyr_3893 at 4635.289 kb on - strand, within Psyr_3893 at 4635.289 kb on - strand, within Psyr_3893 at 4635.317 kb on - strand, within Psyr_3893 at 4635.386 kb on + strand, within Psyr_3893 at 4635.386 kb on + strand, within Psyr_3893 at 4635.386 kb on + strand, within Psyr_3893 at 4635.386 kb on + strand, within Psyr_3893 at 4635.387 kb on - strand, within Psyr_3893 at 4635.398 kb on + strand, within Psyr_3893 at 4635.505 kb on + strand, within Psyr_3893 at 4635.505 kb on + strand, within Psyr_3893 at 4635.505 kb on + strand, within Psyr_3893 at 4635.505 kb on + strand, within Psyr_3893 at 4635.505 kb on + strand, within Psyr_3893 at 4635.506 kb on - strand, within Psyr_3893 at 4635.506 kb on - strand, within Psyr_3893 at 4635.506 kb on - strand, within Psyr_3893
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 15.6 ug/ml remove 4,632,535 - +0.9 4,632,535 - -1.0 4,632,558 + -0.1 4,632,558 + +0.0 4,632,558 + -1.3 4,632,559 - +1.6 4,632,570 + -0.6 4,632,574 + +0.6 4,632,575 - +0.2 4,632,575 - -0.6 4,632,626 + Psyr_3891 0.12 +0.3 4,632,626 + Psyr_3891 0.12 +1.0 4,632,627 - Psyr_3891 0.12 +0.8 4,632,751 - Psyr_3891 0.36 -0.0 4,632,803 + Psyr_3891 0.46 +0.3 4,632,804 - Psyr_3891 0.46 +1.2 4,632,825 + Psyr_3891 0.50 +0.5 4,632,826 - Psyr_3891 0.50 +0.3 4,632,858 + Psyr_3891 0.56 +0.8 4,632,859 - Psyr_3891 0.56 -0.5 4,632,922 - Psyr_3891 0.68 +0.3 4,633,025 + Psyr_3891 0.88 +0.6 4,633,121 + -0.8 4,633,122 - -0.8 4,633,125 + -0.9 4,633,125 + +0.8 4,633,126 - +1.0 4,633,126 - +0.1 4,633,153 - -0.3 4,633,183 + -0.2 4,633,204 - +0.6 4,633,227 - -1.1 4,633,227 - -1.2 4,633,304 - -0.1 4,633,339 + +1.5 4,633,339 + +0.3 4,633,339 + +0.5 4,633,339 + +1.0 4,633,339 + +0.2 4,633,339 + -1.1 4,633,339 + -1.0 4,633,339 + -0.2 4,633,340 - -0.2 4,633,340 - -0.8 4,633,340 - -0.3 4,633,340 - -1.7 4,633,342 - -0.2 4,633,342 - +0.7 4,633,342 - -1.6 4,633,419 + +0.9 4,633,419 + +0.2 4,633,422 + -0.5 4,633,422 + -1.6 4,633,422 + -0.0 4,634,804 - -0.5 4,634,812 + +1.7 4,634,812 + +1.3 4,635,001 + Psyr_3893 0.21 +0.6 4,635,001 + Psyr_3893 0.21 +0.5 4,635,002 - Psyr_3893 0.21 +1.3 4,635,288 + Psyr_3893 0.53 -0.2 4,635,289 - Psyr_3893 0.53 +1.5 4,635,289 - Psyr_3893 0.53 +1.0 4,635,289 - Psyr_3893 0.53 +0.3 4,635,317 - Psyr_3893 0.56 -0.5 4,635,386 + Psyr_3893 0.64 +0.5 4,635,386 + Psyr_3893 0.64 +0.2 4,635,386 + Psyr_3893 0.64 +1.0 4,635,386 + Psyr_3893 0.64 -0.9 4,635,387 - Psyr_3893 0.64 +1.4 4,635,398 + Psyr_3893 0.65 -1.6 4,635,505 + Psyr_3893 0.77 +1.3 4,635,505 + Psyr_3893 0.77 +1.7 4,635,505 + Psyr_3893 0.77 +0.6 4,635,505 + Psyr_3893 0.77 -0.3 4,635,505 + Psyr_3893 0.77 -0.6 4,635,506 - Psyr_3893 0.77 -0.1 4,635,506 - Psyr_3893 0.77 +0.1 4,635,506 - Psyr_3893 0.77 +0.1
Or see this region's nucleotide sequence