Experiment: KB with Berberine 15.6 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3022 and Psyr_3023 are separated by 493 nucleotides Psyr_3023 and Psyr_3024 are separated by 100 nucleotides
Psyr_3022: Psyr_3022 - transcriptional regulator, AsnC family, at 3,619,950 to 3,620,417
_3022
Psyr_3023: Psyr_3023 - conserved hypothetical protein, at 3,620,911 to 3,621,462
_3023
Psyr_3024: Psyr_3024 - Peptidase M14, carboxypeptidase A, at 3,621,563 to 3,622,699
_3024
Position (kb)
3620
3621
3622 Strain fitness (log2 ratio)
-1
0
1
2 at 3620.415 kb on + strand at 3620.415 kb on + strand at 3620.423 kb on - strand at 3620.423 kb on - strand at 3620.424 kb on + strand at 3620.424 kb on + strand at 3620.424 kb on + strand at 3620.425 kb on - strand at 3620.425 kb on - strand at 3620.425 kb on - strand at 3620.425 kb on - strand at 3620.574 kb on + strand at 3620.787 kb on + strand at 3620.842 kb on + strand at 3620.843 kb on - strand at 3620.905 kb on + strand at 3620.905 kb on + strand at 3620.905 kb on + strand at 3620.906 kb on - strand at 3620.906 kb on - strand at 3620.933 kb on + strand at 3620.934 kb on - strand at 3620.934 kb on - strand at 3621.033 kb on - strand, within Psyr_3023 at 3621.033 kb on - strand, within Psyr_3023 at 3621.033 kb on - strand, within Psyr_3023 at 3621.033 kb on - strand, within Psyr_3023 at 3621.033 kb on - strand, within Psyr_3023 at 3621.064 kb on + strand, within Psyr_3023 at 3621.064 kb on + strand, within Psyr_3023 at 3621.214 kb on + strand, within Psyr_3023 at 3621.215 kb on - strand, within Psyr_3023 at 3621.215 kb on - strand, within Psyr_3023 at 3621.215 kb on - strand, within Psyr_3023 at 3621.394 kb on + strand, within Psyr_3023 at 3621.395 kb on - strand, within Psyr_3023 at 3621.491 kb on - strand at 3621.519 kb on + strand at 3621.520 kb on - strand at 3621.520 kb on - strand at 3621.520 kb on - strand at 3621.520 kb on - strand at 3621.544 kb on + strand at 3621.545 kb on - strand at 3621.545 kb on - strand at 3621.559 kb on + strand at 3621.559 kb on + strand at 3621.560 kb on - strand at 3621.560 kb on - strand at 3621.560 kb on - strand at 3621.560 kb on - strand at 3621.560 kb on - strand at 3621.723 kb on - strand, within Psyr_3024 at 3621.780 kb on - strand, within Psyr_3024 at 3621.906 kb on - strand, within Psyr_3024 at 3622.001 kb on - strand, within Psyr_3024 at 3622.173 kb on - strand, within Psyr_3024 at 3622.367 kb on + strand, within Psyr_3024 at 3622.371 kb on + strand, within Psyr_3024 at 3622.371 kb on + strand, within Psyr_3024 at 3622.372 kb on - strand, within Psyr_3024 at 3622.372 kb on - strand, within Psyr_3024 at 3622.372 kb on - strand, within Psyr_3024 at 3622.372 kb on - strand, within Psyr_3024 at 3622.403 kb on + strand, within Psyr_3024
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 15.6 ug/ml remove 3,620,415 + -0.5 3,620,415 + +0.2 3,620,423 - +0.9 3,620,423 - +0.9 3,620,424 + -0.3 3,620,424 + +0.2 3,620,424 + -0.1 3,620,425 - -0.1 3,620,425 - +0.7 3,620,425 - +0.4 3,620,425 - +0.0 3,620,574 + -0.3 3,620,787 + +0.1 3,620,842 + +0.6 3,620,843 - +0.2 3,620,905 + +0.5 3,620,905 + -0.4 3,620,905 + -0.5 3,620,906 - -0.8 3,620,906 - -0.7 3,620,933 + +0.7 3,620,934 - +0.9 3,620,934 - -0.0 3,621,033 - Psyr_3023 0.22 -0.5 3,621,033 - Psyr_3023 0.22 +1.9 3,621,033 - Psyr_3023 0.22 -0.3 3,621,033 - Psyr_3023 0.22 -0.1 3,621,033 - Psyr_3023 0.22 +0.4 3,621,064 + Psyr_3023 0.28 +0.0 3,621,064 + Psyr_3023 0.28 -1.0 3,621,214 + Psyr_3023 0.55 +0.1 3,621,215 - Psyr_3023 0.55 +1.5 3,621,215 - Psyr_3023 0.55 +0.9 3,621,215 - Psyr_3023 0.55 -0.1 3,621,394 + Psyr_3023 0.88 +1.1 3,621,395 - Psyr_3023 0.88 -0.7 3,621,491 - +0.0 3,621,519 + +2.5 3,621,520 - +1.6 3,621,520 - +0.7 3,621,520 - -0.2 3,621,520 - -0.4 3,621,544 + -0.0 3,621,545 - -1.2 3,621,545 - +0.7 3,621,559 + -0.6 3,621,559 + +1.1 3,621,560 - -1.4 3,621,560 - -0.0 3,621,560 - +0.3 3,621,560 - +0.1 3,621,560 - +0.3 3,621,723 - Psyr_3024 0.14 -0.7 3,621,780 - Psyr_3024 0.19 -0.3 3,621,906 - Psyr_3024 0.30 -0.6 3,622,001 - Psyr_3024 0.39 +0.5 3,622,173 - Psyr_3024 0.54 -0.1 3,622,367 + Psyr_3024 0.71 -0.8 3,622,371 + Psyr_3024 0.71 -1.7 3,622,371 + Psyr_3024 0.71 -0.9 3,622,372 - Psyr_3024 0.71 -1.0 3,622,372 - Psyr_3024 0.71 -0.7 3,622,372 - Psyr_3024 0.71 -0.7 3,622,372 - Psyr_3024 0.71 +0.2 3,622,403 + Psyr_3024 0.74 -0.0
Or see this region's nucleotide sequence