Experiment: KB with Berberine 15.6 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1102 and Psyr_1103 are separated by 98 nucleotides Psyr_1103 and Psyr_1104 overlap by 4 nucleotides Psyr_1104 and Psyr_1105 are separated by 116 nucleotides
Psyr_1102: Psyr_1102 - conserved hypothetical protein, at 1,248,790 to 1,249,146
_1102
Psyr_1103: Psyr_1103 - Protein of unknown function DUF454, at 1,249,245 to 1,249,703
_1103
Psyr_1104: Psyr_1104 - Heme oxygenase, at 1,249,700 to 1,250,314
_1104
Psyr_1105: Psyr_1105 - TonB-dependent hemoglobin/transferrin/lactoferrin receptor:TonB-dependent heme/hemoglobin receptor, at 1,250,431 to 1,253,010
_1105
Position (kb)
1249
1250 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1248.399 kb on + strand at 1248.458 kb on - strand at 1248.506 kb on + strand at 1248.696 kb on + strand at 1248.869 kb on + strand, within Psyr_1102 at 1248.869 kb on + strand, within Psyr_1102 at 1248.870 kb on - strand, within Psyr_1102 at 1249.068 kb on - strand, within Psyr_1102 at 1249.068 kb on - strand, within Psyr_1102 at 1249.085 kb on + strand, within Psyr_1102 at 1249.086 kb on - strand, within Psyr_1102 at 1249.086 kb on - strand, within Psyr_1102 at 1249.086 kb on - strand, within Psyr_1102 at 1249.086 kb on - strand, within Psyr_1102 at 1249.086 kb on - strand, within Psyr_1102 at 1249.086 kb on - strand, within Psyr_1102 at 1249.142 kb on + strand at 1249.142 kb on + strand at 1249.143 kb on - strand at 1249.143 kb on - strand at 1249.143 kb on - strand at 1249.255 kb on - strand at 1249.255 kb on - strand at 1249.255 kb on - strand at 1249.287 kb on + strand at 1249.287 kb on + strand at 1249.287 kb on + strand at 1249.287 kb on + strand at 1249.288 kb on - strand at 1249.321 kb on + strand, within Psyr_1103 at 1249.322 kb on - strand, within Psyr_1103 at 1249.322 kb on - strand, within Psyr_1103 at 1249.435 kb on + strand, within Psyr_1103 at 1249.435 kb on + strand, within Psyr_1103 at 1249.435 kb on + strand, within Psyr_1103 at 1249.435 kb on + strand, within Psyr_1103 at 1249.435 kb on + strand, within Psyr_1103 at 1249.436 kb on - strand, within Psyr_1103 at 1249.436 kb on - strand, within Psyr_1103 at 1249.436 kb on - strand, within Psyr_1103 at 1249.436 kb on - strand, within Psyr_1103 at 1249.569 kb on + strand, within Psyr_1103 at 1249.602 kb on + strand, within Psyr_1103 at 1249.602 kb on + strand, within Psyr_1103 at 1249.602 kb on + strand, within Psyr_1103 at 1249.602 kb on + strand, within Psyr_1103 at 1249.602 kb on + strand, within Psyr_1103 at 1249.603 kb on - strand, within Psyr_1103 at 1249.603 kb on - strand, within Psyr_1103 at 1249.603 kb on - strand, within Psyr_1103 at 1249.636 kb on + strand, within Psyr_1103 at 1249.636 kb on + strand, within Psyr_1103 at 1249.636 kb on + strand, within Psyr_1103 at 1249.636 kb on + strand, within Psyr_1103 at 1249.636 kb on + strand, within Psyr_1103 at 1249.636 kb on + strand, within Psyr_1103 at 1249.636 kb on + strand, within Psyr_1103 at 1249.637 kb on - strand, within Psyr_1103 at 1249.637 kb on - strand, within Psyr_1103 at 1250.136 kb on + strand, within Psyr_1104 at 1250.136 kb on + strand, within Psyr_1104 at 1250.166 kb on + strand, within Psyr_1104 at 1250.190 kb on + strand, within Psyr_1104 at 1250.191 kb on - strand, within Psyr_1104 at 1250.317 kb on + strand at 1250.349 kb on + strand at 1250.375 kb on + strand at 1250.507 kb on - strand at 1250.579 kb on + strand at 1250.579 kb on + strand at 1250.579 kb on + strand at 1250.579 kb on + strand at 1250.580 kb on - strand at 1250.618 kb on + strand at 1250.618 kb on + strand at 1250.618 kb on + strand at 1250.618 kb on + strand at 1250.618 kb on + strand at 1250.618 kb on + strand at 1250.618 kb on + strand at 1250.619 kb on - strand at 1250.619 kb on - strand at 1250.619 kb on - strand at 1250.619 kb on - strand at 1250.619 kb on - strand at 1250.619 kb on - strand at 1250.619 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 15.6 ug/ml remove 1,248,399 + +0.3 1,248,458 - +1.2 1,248,506 + +0.5 1,248,696 + -0.2 1,248,869 + Psyr_1102 0.22 +0.2 1,248,869 + Psyr_1102 0.22 +0.6 1,248,870 - Psyr_1102 0.22 +0.9 1,249,068 - Psyr_1102 0.78 +0.8 1,249,068 - Psyr_1102 0.78 -2.0 1,249,085 + Psyr_1102 0.83 +0.7 1,249,086 - Psyr_1102 0.83 -0.6 1,249,086 - Psyr_1102 0.83 -1.8 1,249,086 - Psyr_1102 0.83 +0.8 1,249,086 - Psyr_1102 0.83 -0.8 1,249,086 - Psyr_1102 0.83 -0.3 1,249,086 - Psyr_1102 0.83 -0.7 1,249,142 + +0.1 1,249,142 + -0.3 1,249,143 - +1.6 1,249,143 - -0.3 1,249,143 - +1.0 1,249,255 - +0.4 1,249,255 - -0.2 1,249,255 - +0.7 1,249,287 + -0.3 1,249,287 + +1.0 1,249,287 + +0.1 1,249,287 + +1.0 1,249,288 - +0.7 1,249,321 + Psyr_1103 0.17 -0.0 1,249,322 - Psyr_1103 0.17 -0.8 1,249,322 - Psyr_1103 0.17 -0.8 1,249,435 + Psyr_1103 0.41 +0.1 1,249,435 + Psyr_1103 0.41 +0.6 1,249,435 + Psyr_1103 0.41 -0.6 1,249,435 + Psyr_1103 0.41 +0.4 1,249,435 + Psyr_1103 0.41 -0.0 1,249,436 - Psyr_1103 0.42 +2.6 1,249,436 - Psyr_1103 0.42 +0.2 1,249,436 - Psyr_1103 0.42 +0.7 1,249,436 - Psyr_1103 0.42 -0.3 1,249,569 + Psyr_1103 0.71 -0.8 1,249,602 + Psyr_1103 0.78 -0.3 1,249,602 + Psyr_1103 0.78 +0.7 1,249,602 + Psyr_1103 0.78 +0.4 1,249,602 + Psyr_1103 0.78 -1.3 1,249,602 + Psyr_1103 0.78 +0.2 1,249,603 - Psyr_1103 0.78 +0.4 1,249,603 - Psyr_1103 0.78 -1.0 1,249,603 - Psyr_1103 0.78 -0.0 1,249,636 + Psyr_1103 0.85 +0.1 1,249,636 + Psyr_1103 0.85 +1.0 1,249,636 + Psyr_1103 0.85 +0.3 1,249,636 + Psyr_1103 0.85 -0.9 1,249,636 + Psyr_1103 0.85 +0.6 1,249,636 + Psyr_1103 0.85 -1.0 1,249,636 + Psyr_1103 0.85 -1.8 1,249,637 - Psyr_1103 0.85 -0.0 1,249,637 - Psyr_1103 0.85 -1.3 1,250,136 + Psyr_1104 0.71 +0.6 1,250,136 + Psyr_1104 0.71 +1.4 1,250,166 + Psyr_1104 0.76 +1.3 1,250,190 + Psyr_1104 0.80 +1.1 1,250,191 - Psyr_1104 0.80 -0.2 1,250,317 + +0.4 1,250,349 + -2.0 1,250,375 + +1.0 1,250,507 - -0.3 1,250,579 + -1.2 1,250,579 + +1.4 1,250,579 + -2.0 1,250,579 + -0.6 1,250,580 - +0.5 1,250,618 + +0.5 1,250,618 + -0.0 1,250,618 + +0.4 1,250,618 + -0.6 1,250,618 + +0.3 1,250,618 + -0.1 1,250,618 + +1.2 1,250,619 - -0.6 1,250,619 - +1.7 1,250,619 - +0.8 1,250,619 - +0.0 1,250,619 - +0.3 1,250,619 - -0.1 1,250,619 - -1.9
Or see this region's nucleotide sequence