Experiment: KB with Berberine 15.6 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1086 and Psyr_1087 are separated by 123 nucleotides Psyr_1087 and Psyr_1088 overlap by 4 nucleotides Psyr_1088 and Psyr_1089 are separated by 43 nucleotides
Psyr_1086: Psyr_1086 - valyl-tRNA synthetase, at 1,227,714 to 1,230,560
_1086
Psyr_1087: Psyr_1087 - CDS, at 1,230,684 to 1,230,956
_1087
Psyr_1088: Psyr_1088 - CDS, at 1,230,953 to 1,231,684
_1088
Psyr_1089: Psyr_1089 - conserved hypothetical protein, at 1,231,728 to 1,232,162
_1089
Position (kb)
1230
1231 Strain fitness (log2 ratio)
-1
0
1
2 at 1230.603 kb on - strand at 1230.605 kb on - strand at 1230.605 kb on - strand at 1230.664 kb on + strand at 1230.664 kb on + strand at 1230.665 kb on - strand at 1230.719 kb on + strand, within Psyr_1087 at 1230.719 kb on + strand, within Psyr_1087 at 1230.720 kb on - strand, within Psyr_1087 at 1230.720 kb on - strand, within Psyr_1087 at 1230.786 kb on + strand, within Psyr_1087 at 1230.877 kb on + strand, within Psyr_1087 at 1230.878 kb on - strand, within Psyr_1087 at 1231.064 kb on + strand, within Psyr_1088 at 1231.065 kb on - strand, within Psyr_1088 at 1231.070 kb on + strand, within Psyr_1088 at 1231.070 kb on + strand, within Psyr_1088 at 1231.070 kb on + strand, within Psyr_1088 at 1231.071 kb on - strand, within Psyr_1088 at 1231.128 kb on + strand, within Psyr_1088 at 1231.128 kb on + strand, within Psyr_1088 at 1231.128 kb on + strand, within Psyr_1088 at 1231.128 kb on + strand, within Psyr_1088 at 1231.129 kb on - strand, within Psyr_1088 at 1231.129 kb on - strand, within Psyr_1088 at 1231.129 kb on - strand, within Psyr_1088 at 1231.129 kb on - strand, within Psyr_1088 at 1231.170 kb on + strand, within Psyr_1088 at 1231.171 kb on - strand, within Psyr_1088 at 1231.171 kb on - strand, within Psyr_1088 at 1231.219 kb on + strand, within Psyr_1088 at 1231.219 kb on + strand, within Psyr_1088 at 1231.219 kb on + strand, within Psyr_1088 at 1231.222 kb on + strand, within Psyr_1088 at 1231.222 kb on + strand, within Psyr_1088 at 1231.222 kb on + strand, within Psyr_1088 at 1231.222 kb on + strand, within Psyr_1088 at 1231.222 kb on + strand, within Psyr_1088 at 1231.222 kb on + strand, within Psyr_1088 at 1231.222 kb on + strand, within Psyr_1088 at 1231.222 kb on + strand, within Psyr_1088 at 1231.223 kb on - strand, within Psyr_1088 at 1231.223 kb on - strand, within Psyr_1088 at 1231.223 kb on - strand, within Psyr_1088 at 1231.223 kb on - strand, within Psyr_1088 at 1231.263 kb on + strand, within Psyr_1088 at 1231.264 kb on - strand, within Psyr_1088 at 1231.267 kb on + strand, within Psyr_1088 at 1231.267 kb on + strand, within Psyr_1088 at 1231.278 kb on + strand, within Psyr_1088 at 1231.299 kb on + strand, within Psyr_1088 at 1231.300 kb on - strand, within Psyr_1088 at 1231.300 kb on - strand, within Psyr_1088 at 1231.321 kb on + strand, within Psyr_1088 at 1231.322 kb on - strand, within Psyr_1088 at 1231.322 kb on - strand, within Psyr_1088 at 1231.324 kb on - strand, within Psyr_1088 at 1231.353 kb on + strand, within Psyr_1088 at 1231.353 kb on + strand, within Psyr_1088 at 1231.353 kb on + strand, within Psyr_1088 at 1231.354 kb on - strand, within Psyr_1088 at 1231.354 kb on - strand, within Psyr_1088 at 1231.354 kb on - strand, within Psyr_1088 at 1231.354 kb on - strand, within Psyr_1088 at 1231.354 kb on - strand, within Psyr_1088 at 1231.354 kb on - strand, within Psyr_1088 at 1231.486 kb on + strand, within Psyr_1088 at 1231.522 kb on + strand, within Psyr_1088 at 1231.522 kb on + strand, within Psyr_1088 at 1231.523 kb on - strand, within Psyr_1088 at 1231.560 kb on + strand, within Psyr_1088 at 1231.565 kb on - strand, within Psyr_1088 at 1231.567 kb on + strand, within Psyr_1088 at 1231.568 kb on - strand, within Psyr_1088 at 1231.570 kb on + strand, within Psyr_1088 at 1231.570 kb on + strand, within Psyr_1088 at 1231.570 kb on + strand, within Psyr_1088 at 1231.571 kb on - strand, within Psyr_1088 at 1231.669 kb on - strand at 1231.694 kb on + strand at 1231.694 kb on + strand at 1231.694 kb on + strand at 1231.694 kb on + strand at 1231.694 kb on + strand at 1231.695 kb on - strand at 1231.695 kb on - strand at 1231.695 kb on - strand at 1231.695 kb on - strand at 1231.951 kb on - strand, within Psyr_1089
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 15.6 ug/ml remove 1,230,603 - -0.4 1,230,605 - +1.0 1,230,605 - -0.9 1,230,664 + -0.4 1,230,664 + -0.0 1,230,665 - +0.9 1,230,719 + Psyr_1087 0.13 +1.3 1,230,719 + Psyr_1087 0.13 -0.7 1,230,720 - Psyr_1087 0.13 -1.1 1,230,720 - Psyr_1087 0.13 -0.8 1,230,786 + Psyr_1087 0.37 +0.5 1,230,877 + Psyr_1087 0.71 +0.5 1,230,878 - Psyr_1087 0.71 -0.9 1,231,064 + Psyr_1088 0.15 -0.8 1,231,065 - Psyr_1088 0.15 +0.1 1,231,070 + Psyr_1088 0.16 -0.6 1,231,070 + Psyr_1088 0.16 -1.6 1,231,070 + Psyr_1088 0.16 -0.0 1,231,071 - Psyr_1088 0.16 -1.2 1,231,128 + Psyr_1088 0.24 +0.5 1,231,128 + Psyr_1088 0.24 +0.1 1,231,128 + Psyr_1088 0.24 -0.0 1,231,128 + Psyr_1088 0.24 +0.2 1,231,129 - Psyr_1088 0.24 +0.3 1,231,129 - Psyr_1088 0.24 -1.3 1,231,129 - Psyr_1088 0.24 -0.0 1,231,129 - Psyr_1088 0.24 -0.1 1,231,170 + Psyr_1088 0.30 +1.3 1,231,171 - Psyr_1088 0.30 -0.9 1,231,171 - Psyr_1088 0.30 +0.1 1,231,219 + Psyr_1088 0.36 -1.0 1,231,219 + Psyr_1088 0.36 +1.4 1,231,219 + Psyr_1088 0.36 +0.3 1,231,222 + Psyr_1088 0.37 -0.8 1,231,222 + Psyr_1088 0.37 +0.3 1,231,222 + Psyr_1088 0.37 +1.0 1,231,222 + Psyr_1088 0.37 +2.0 1,231,222 + Psyr_1088 0.37 +1.4 1,231,222 + Psyr_1088 0.37 -0.4 1,231,222 + Psyr_1088 0.37 -0.3 1,231,222 + Psyr_1088 0.37 -0.6 1,231,223 - Psyr_1088 0.37 -0.7 1,231,223 - Psyr_1088 0.37 -0.3 1,231,223 - Psyr_1088 0.37 -1.1 1,231,223 - Psyr_1088 0.37 -1.1 1,231,263 + Psyr_1088 0.42 -1.0 1,231,264 - Psyr_1088 0.42 -0.0 1,231,267 + Psyr_1088 0.43 -0.0 1,231,267 + Psyr_1088 0.43 +0.1 1,231,278 + Psyr_1088 0.44 +0.9 1,231,299 + Psyr_1088 0.47 +0.4 1,231,300 - Psyr_1088 0.47 -0.7 1,231,300 - Psyr_1088 0.47 -0.7 1,231,321 + Psyr_1088 0.50 -0.2 1,231,322 - Psyr_1088 0.50 +0.2 1,231,322 - Psyr_1088 0.50 +1.3 1,231,324 - Psyr_1088 0.51 -0.3 1,231,353 + Psyr_1088 0.55 +1.2 1,231,353 + Psyr_1088 0.55 -0.6 1,231,353 + Psyr_1088 0.55 +1.1 1,231,354 - Psyr_1088 0.55 -0.5 1,231,354 - Psyr_1088 0.55 +1.0 1,231,354 - Psyr_1088 0.55 -1.3 1,231,354 - Psyr_1088 0.55 +0.6 1,231,354 - Psyr_1088 0.55 -0.0 1,231,354 - Psyr_1088 0.55 +0.1 1,231,486 + Psyr_1088 0.73 +0.3 1,231,522 + Psyr_1088 0.78 +0.7 1,231,522 + Psyr_1088 0.78 +0.1 1,231,523 - Psyr_1088 0.78 -0.1 1,231,560 + Psyr_1088 0.83 -0.0 1,231,565 - Psyr_1088 0.84 +1.0 1,231,567 + Psyr_1088 0.84 +0.1 1,231,568 - Psyr_1088 0.84 +0.4 1,231,570 + Psyr_1088 0.84 +0.0 1,231,570 + Psyr_1088 0.84 +0.7 1,231,570 + Psyr_1088 0.84 -0.7 1,231,571 - Psyr_1088 0.84 -1.0 1,231,669 - -1.2 1,231,694 + -0.3 1,231,694 + +0.5 1,231,694 + +0.8 1,231,694 + -0.4 1,231,694 + -0.3 1,231,695 - +0.4 1,231,695 - -0.6 1,231,695 - +0.5 1,231,695 - -1.0 1,231,951 - Psyr_1089 0.51 -0.2
Or see this region's nucleotide sequence