Experiment: KB with Berberine 15.6 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0799 and Psyr_0800 are separated by 174 nucleotides Psyr_0800 and Psyr_0801 are separated by 119 nucleotides Psyr_0801 and Psyr_0802 are separated by 54 nucleotides
Psyr_0799: Psyr_0799 - Fimbrial protein pilin, at 905,429 to 905,848
_0799
Psyr_0800: Psyr_0800 - Protein of unknown function DUF1523, at 906,023 to 906,568
_0800
Psyr_0801: Psyr_0801 - conserved hypothetical protein, at 906,688 to 907,686
_0801
Psyr_0802: Psyr_0802 - Peptidase S1, chymotrypsin, at 907,741 to 908,880
_0802
Position (kb)
906
907
908 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 905.716 kb on + strand, within Psyr_0799 at 905.716 kb on + strand, within Psyr_0799 at 905.722 kb on + strand, within Psyr_0799 at 905.723 kb on - strand, within Psyr_0799 at 905.723 kb on - strand, within Psyr_0799 at 905.725 kb on + strand, within Psyr_0799 at 905.779 kb on + strand, within Psyr_0799 at 905.861 kb on + strand at 905.862 kb on - strand at 905.862 kb on - strand at 905.862 kb on - strand at 905.882 kb on - strand at 905.882 kb on - strand at 906.007 kb on + strand at 906.059 kb on - strand at 906.059 kb on - strand at 906.216 kb on + strand, within Psyr_0800 at 906.220 kb on - strand, within Psyr_0800 at 906.301 kb on - strand, within Psyr_0800 at 906.381 kb on + strand, within Psyr_0800 at 906.381 kb on + strand, within Psyr_0800 at 906.381 kb on + strand, within Psyr_0800 at 906.381 kb on + strand, within Psyr_0800 at 906.381 kb on + strand, within Psyr_0800 at 906.381 kb on + strand, within Psyr_0800 at 906.381 kb on + strand, within Psyr_0800 at 906.381 kb on + strand, within Psyr_0800 at 906.382 kb on - strand, within Psyr_0800 at 906.495 kb on + strand, within Psyr_0800 at 906.495 kb on + strand, within Psyr_0800 at 906.495 kb on + strand, within Psyr_0800 at 906.495 kb on + strand, within Psyr_0800 at 906.496 kb on - strand, within Psyr_0800 at 906.496 kb on - strand, within Psyr_0800 at 906.496 kb on - strand, within Psyr_0800 at 906.496 kb on - strand, within Psyr_0800 at 906.496 kb on - strand, within Psyr_0800 at 906.496 kb on - strand, within Psyr_0800 at 906.498 kb on + strand, within Psyr_0800 at 906.499 kb on - strand, within Psyr_0800 at 906.499 kb on - strand, within Psyr_0800 at 906.568 kb on + strand at 906.569 kb on - strand at 906.569 kb on - strand at 906.586 kb on - strand at 906.634 kb on + strand at 906.635 kb on - strand at 906.635 kb on - strand at 906.637 kb on + strand at 906.734 kb on + strand at 906.900 kb on + strand, within Psyr_0801 at 906.915 kb on + strand, within Psyr_0801 at 906.918 kb on - strand, within Psyr_0801 at 906.928 kb on + strand, within Psyr_0801 at 906.929 kb on - strand, within Psyr_0801 at 906.929 kb on - strand, within Psyr_0801 at 906.929 kb on - strand, within Psyr_0801 at 907.063 kb on - strand, within Psyr_0801 at 907.108 kb on + strand, within Psyr_0801 at 907.108 kb on + strand, within Psyr_0801 at 907.109 kb on - strand, within Psyr_0801 at 907.109 kb on - strand, within Psyr_0801 at 907.163 kb on - strand, within Psyr_0801 at 907.253 kb on - strand, within Psyr_0801 at 907.257 kb on + strand, within Psyr_0801 at 907.257 kb on + strand, within Psyr_0801 at 907.257 kb on + strand, within Psyr_0801 at 907.257 kb on + strand, within Psyr_0801 at 907.306 kb on + strand, within Psyr_0801 at 907.306 kb on + strand, within Psyr_0801 at 907.306 kb on + strand, within Psyr_0801 at 907.306 kb on + strand, within Psyr_0801 at 907.307 kb on - strand, within Psyr_0801 at 907.307 kb on - strand, within Psyr_0801 at 907.424 kb on + strand, within Psyr_0801 at 907.424 kb on + strand, within Psyr_0801 at 907.424 kb on + strand, within Psyr_0801 at 907.424 kb on + strand, within Psyr_0801 at 907.424 kb on + strand, within Psyr_0801 at 907.425 kb on - strand, within Psyr_0801 at 907.534 kb on + strand, within Psyr_0801 at 907.552 kb on + strand, within Psyr_0801 at 907.723 kb on + strand at 907.775 kb on + strand at 907.775 kb on + strand at 907.776 kb on - strand at 907.813 kb on - strand at 907.834 kb on + strand at 907.835 kb on - strand at 908.008 kb on + strand, within Psyr_0802 at 908.008 kb on + strand, within Psyr_0802 at 908.008 kb on + strand, within Psyr_0802 at 908.008 kb on + strand, within Psyr_0802 at 908.009 kb on - strand, within Psyr_0802 at 908.009 kb on - strand, within Psyr_0802 at 908.009 kb on - strand, within Psyr_0802 at 908.009 kb on - strand, within Psyr_0802 at 908.009 kb on - strand, within Psyr_0802 at 908.009 kb on - strand, within Psyr_0802 at 908.009 kb on - strand, within Psyr_0802 at 908.009 kb on - strand, within Psyr_0802 at 908.009 kb on - strand, within Psyr_0802 at 908.014 kb on + strand, within Psyr_0802 at 908.015 kb on - strand, within Psyr_0802 at 908.365 kb on + strand, within Psyr_0802 at 908.365 kb on + strand, within Psyr_0802 at 908.365 kb on + strand, within Psyr_0802 at 908.366 kb on - strand, within Psyr_0802 at 908.366 kb on - strand, within Psyr_0802 at 908.366 kb on - strand, within Psyr_0802 at 908.473 kb on - strand, within Psyr_0802 at 908.637 kb on - strand, within Psyr_0802
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 15.6 ug/ml remove 905,716 + Psyr_0799 0.68 -0.3 905,716 + Psyr_0799 0.68 +0.5 905,722 + Psyr_0799 0.70 -0.6 905,723 - Psyr_0799 0.70 -0.4 905,723 - Psyr_0799 0.70 +0.4 905,725 + Psyr_0799 0.70 +0.7 905,779 + Psyr_0799 0.83 +2.4 905,861 + +2.5 905,862 - -0.9 905,862 - -0.1 905,862 - -0.7 905,882 - +0.0 905,882 - +0.5 906,007 + +1.0 906,059 - -0.5 906,059 - -0.0 906,216 + Psyr_0800 0.35 -0.0 906,220 - Psyr_0800 0.36 +0.5 906,301 - Psyr_0800 0.51 +3.0 906,381 + Psyr_0800 0.66 -0.5 906,381 + Psyr_0800 0.66 +0.5 906,381 + Psyr_0800 0.66 -1.0 906,381 + Psyr_0800 0.66 +0.2 906,381 + Psyr_0800 0.66 +0.4 906,381 + Psyr_0800 0.66 +0.2 906,381 + Psyr_0800 0.66 +0.3 906,381 + Psyr_0800 0.66 -2.0 906,382 - Psyr_0800 0.66 +0.4 906,495 + Psyr_0800 0.86 -1.4 906,495 + Psyr_0800 0.86 +1.3 906,495 + Psyr_0800 0.86 -0.9 906,495 + Psyr_0800 0.86 +2.2 906,496 - Psyr_0800 0.87 +0.1 906,496 - Psyr_0800 0.87 -0.4 906,496 - Psyr_0800 0.87 -0.3 906,496 - Psyr_0800 0.87 -1.1 906,496 - Psyr_0800 0.87 +1.0 906,496 - Psyr_0800 0.87 -0.1 906,498 + Psyr_0800 0.87 -0.5 906,499 - Psyr_0800 0.87 +0.8 906,499 - Psyr_0800 0.87 +0.2 906,568 + +1.8 906,569 - +0.1 906,569 - +0.0 906,586 - +1.0 906,634 + -0.3 906,635 - +1.8 906,635 - -1.0 906,637 + -0.2 906,734 + -1.2 906,900 + Psyr_0801 0.21 +0.6 906,915 + Psyr_0801 0.23 -0.5 906,918 - Psyr_0801 0.23 -0.2 906,928 + Psyr_0801 0.24 -2.2 906,929 - Psyr_0801 0.24 -0.2 906,929 - Psyr_0801 0.24 +0.2 906,929 - Psyr_0801 0.24 -0.2 907,063 - Psyr_0801 0.38 -0.6 907,108 + Psyr_0801 0.42 +0.2 907,108 + Psyr_0801 0.42 -0.2 907,109 - Psyr_0801 0.42 +1.5 907,109 - Psyr_0801 0.42 -0.0 907,163 - Psyr_0801 0.48 -0.5 907,253 - Psyr_0801 0.57 +0.5 907,257 + Psyr_0801 0.57 -0.6 907,257 + Psyr_0801 0.57 -0.5 907,257 + Psyr_0801 0.57 -1.4 907,257 + Psyr_0801 0.57 -0.5 907,306 + Psyr_0801 0.62 -0.3 907,306 + Psyr_0801 0.62 +0.7 907,306 + Psyr_0801 0.62 +1.1 907,306 + Psyr_0801 0.62 +0.5 907,307 - Psyr_0801 0.62 -0.7 907,307 - Psyr_0801 0.62 +0.3 907,424 + Psyr_0801 0.74 +0.3 907,424 + Psyr_0801 0.74 +0.4 907,424 + Psyr_0801 0.74 -0.4 907,424 + Psyr_0801 0.74 -0.4 907,424 + Psyr_0801 0.74 +1.0 907,425 - Psyr_0801 0.74 +0.3 907,534 + Psyr_0801 0.85 +0.4 907,552 + Psyr_0801 0.86 -0.0 907,723 + +0.2 907,775 + +0.6 907,775 + +1.2 907,776 - -1.4 907,813 - +1.0 907,834 + -0.4 907,835 - -0.1 908,008 + Psyr_0802 0.23 -0.6 908,008 + Psyr_0802 0.23 -0.3 908,008 + Psyr_0802 0.23 -0.4 908,008 + Psyr_0802 0.23 +0.2 908,009 - Psyr_0802 0.24 -0.4 908,009 - Psyr_0802 0.24 -1.6 908,009 - Psyr_0802 0.24 -0.3 908,009 - Psyr_0802 0.24 -0.3 908,009 - Psyr_0802 0.24 -0.9 908,009 - Psyr_0802 0.24 +2.5 908,009 - Psyr_0802 0.24 +0.4 908,009 - Psyr_0802 0.24 -1.0 908,009 - Psyr_0802 0.24 +0.3 908,014 + Psyr_0802 0.24 -0.1 908,015 - Psyr_0802 0.24 -0.5 908,365 + Psyr_0802 0.55 -1.4 908,365 + Psyr_0802 0.55 +0.0 908,365 + Psyr_0802 0.55 -0.4 908,366 - Psyr_0802 0.55 -1.9 908,366 - Psyr_0802 0.55 -0.0 908,366 - Psyr_0802 0.55 -0.6 908,473 - Psyr_0802 0.64 -0.1 908,637 - Psyr_0802 0.79 -0.6
Or see this region's nucleotide sequence