Strain Fitness in Pseudomonas fluorescens SBW25 around PFLU_RS12675

Experiment: Trisodium citrate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS12670 and PFLU_RS12675 are separated by 95 nucleotidesPFLU_RS12675 and PFLU_RS12680 are separated by 93 nucleotidesPFLU_RS12680 and PFLU_RS12685 are separated by 28 nucleotidesPFLU_RS12685 and PFLU_RS12690 overlap by 4 nucleotides PFLU_RS12670: PFLU_RS12670 - TonB-dependent receptor, at 2,860,059 to 2,862,185 _RS12670 PFLU_RS12675: PFLU_RS12675 - DUF2946 domain-containing protein, at 2,862,281 to 2,862,670 _RS12675 PFLU_RS12680: PFLU_RS12680 - DUF3995 domain-containing protein, at 2,862,764 to 2,863,201 _RS12680 PFLU_RS12685: PFLU_RS12685 - hypothetical protein, at 2,863,230 to 2,863,562 _RS12685 PFLU_RS12690: PFLU_RS12690 - RHS repeat protein, at 2,863,559 to 2,868,193 _RS12690 Position (kb) 2862 2863Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2861.356 kb on + strand, within PFLU_RS12670at 2861.356 kb on + strand, within PFLU_RS12670at 2861.357 kb on - strand, within PFLU_RS12670at 2861.357 kb on - strand, within PFLU_RS12670at 2861.357 kb on - strand, within PFLU_RS12670at 2861.357 kb on - strand, within PFLU_RS12670at 2861.357 kb on - strand, within PFLU_RS12670at 2861.428 kb on + strand, within PFLU_RS12670at 2861.548 kb on + strand, within PFLU_RS12670at 2861.549 kb on - strand, within PFLU_RS12670at 2861.549 kb on - strand, within PFLU_RS12670at 2861.549 kb on - strand, within PFLU_RS12670at 2861.549 kb on - strand, within PFLU_RS12670at 2861.572 kb on - strand, within PFLU_RS12670at 2861.572 kb on - strand, within PFLU_RS12670at 2861.656 kb on + strand, within PFLU_RS12670at 2861.666 kb on - strand, within PFLU_RS12670at 2861.719 kb on + strand, within PFLU_RS12670at 2861.719 kb on + strand, within PFLU_RS12670at 2861.720 kb on - strand, within PFLU_RS12670at 2861.720 kb on - strand, within PFLU_RS12670at 2861.720 kb on - strand, within PFLU_RS12670at 2861.720 kb on - strand, within PFLU_RS12670at 2861.720 kb on - strand, within PFLU_RS12670at 2861.720 kb on - strand, within PFLU_RS12670at 2861.720 kb on - strand, within PFLU_RS12670at 2861.720 kb on - strand, within PFLU_RS12670at 2861.794 kb on + strand, within PFLU_RS12670at 2861.794 kb on + strand, within PFLU_RS12670at 2861.794 kb on + strand, within PFLU_RS12670at 2861.794 kb on + strand, within PFLU_RS12670at 2861.794 kb on + strand, within PFLU_RS12670at 2861.795 kb on - strand, within PFLU_RS12670at 2861.795 kb on - strand, within PFLU_RS12670at 2861.795 kb on - strand, within PFLU_RS12670at 2861.795 kb on - strand, within PFLU_RS12670at 2861.795 kb on - strand, within PFLU_RS12670at 2861.874 kb on - strand, within PFLU_RS12670at 2861.939 kb on + strand, within PFLU_RS12670at 2861.940 kb on - strand, within PFLU_RS12670at 2862.174 kb on + strandat 2862.174 kb on + strandat 2862.175 kb on - strandat 2862.175 kb on - strandat 2862.175 kb on - strandat 2862.175 kb on - strandat 2862.233 kb on + strandat 2862.233 kb on + strandat 2862.234 kb on - strandat 2862.234 kb on - strandat 2862.472 kb on + strand, within PFLU_RS12675at 2862.473 kb on - strand, within PFLU_RS12675at 2862.473 kb on - strand, within PFLU_RS12675at 2862.533 kb on - strand, within PFLU_RS12675at 2862.683 kb on + strandat 2862.683 kb on + strandat 2862.684 kb on - strandat 2862.730 kb on - strandat 2862.995 kb on + strand, within PFLU_RS12680at 2862.995 kb on + strand, within PFLU_RS12680at 2862.995 kb on + strand, within PFLU_RS12680at 2862.995 kb on + strand, within PFLU_RS12680at 2862.995 kb on + strand, within PFLU_RS12680at 2862.996 kb on - strand, within PFLU_RS12680at 2862.996 kb on - strand, within PFLU_RS12680at 2862.996 kb on - strand, within PFLU_RS12680at 2862.996 kb on - strand, within PFLU_RS12680at 2863.135 kb on + strand, within PFLU_RS12680at 2863.136 kb on - strand, within PFLU_RS12680at 2863.219 kb on + strandat 2863.223 kb on + strandat 2863.223 kb on + strandat 2863.224 kb on - strandat 2863.278 kb on + strand, within PFLU_RS12685at 2863.287 kb on + strand, within PFLU_RS12685at 2863.301 kb on + strand, within PFLU_RS12685at 2863.301 kb on + strand, within PFLU_RS12685at 2863.302 kb on - strand, within PFLU_RS12685at 2863.369 kb on + strand, within PFLU_RS12685at 2863.370 kb on - strand, within PFLU_RS12685at 2863.388 kb on - strand, within PFLU_RS12685at 2863.419 kb on + strand, within PFLU_RS12685at 2863.420 kb on - strand, within PFLU_RS12685at 2863.444 kb on - strand, within PFLU_RS12685at 2863.457 kb on - strand, within PFLU_RS12685at 2863.457 kb on - strand, within PFLU_RS12685at 2863.509 kb on + strand, within PFLU_RS12685at 2863.509 kb on + strand, within PFLU_RS12685at 2863.509 kb on + strand, within PFLU_RS12685at 2863.516 kb on - strand, within PFLU_RS12685at 2863.518 kb on + strand, within PFLU_RS12685at 2863.533 kb on + strandat 2863.533 kb on + strandat 2863.538 kb on + strandat 2863.554 kb on + strandat 2863.583 kb on - strandat 2863.603 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Trisodium citrate (C)
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2,861,356 + PFLU_RS12670 0.61 +0.4
2,861,356 + PFLU_RS12670 0.61 +1.1
2,861,357 - PFLU_RS12670 0.61 -1.4
2,861,357 - PFLU_RS12670 0.61 -0.9
2,861,357 - PFLU_RS12670 0.61 -1.1
2,861,357 - PFLU_RS12670 0.61 +1.2
2,861,357 - PFLU_RS12670 0.61 -0.1
2,861,428 + PFLU_RS12670 0.64 -0.7
2,861,548 + PFLU_RS12670 0.70 +1.1
2,861,549 - PFLU_RS12670 0.70 -0.2
2,861,549 - PFLU_RS12670 0.70 +1.9
2,861,549 - PFLU_RS12670 0.70 -1.0
2,861,549 - PFLU_RS12670 0.70 -0.4
2,861,572 - PFLU_RS12670 0.71 +0.4
2,861,572 - PFLU_RS12670 0.71 -1.8
2,861,656 + PFLU_RS12670 0.75 -0.6
2,861,666 - PFLU_RS12670 0.76 -0.2
2,861,719 + PFLU_RS12670 0.78 -0.5
2,861,719 + PFLU_RS12670 0.78 +0.4
2,861,720 - PFLU_RS12670 0.78 +0.1
2,861,720 - PFLU_RS12670 0.78 +0.8
2,861,720 - PFLU_RS12670 0.78 +1.8
2,861,720 - PFLU_RS12670 0.78 +1.7
2,861,720 - PFLU_RS12670 0.78 +0.8
2,861,720 - PFLU_RS12670 0.78 -2.2
2,861,720 - PFLU_RS12670 0.78 -0.8
2,861,720 - PFLU_RS12670 0.78 +0.5
2,861,794 + PFLU_RS12670 0.82 +0.9
2,861,794 + PFLU_RS12670 0.82 +1.9
2,861,794 + PFLU_RS12670 0.82 +0.0
2,861,794 + PFLU_RS12670 0.82 -0.6
2,861,794 + PFLU_RS12670 0.82 +0.6
2,861,795 - PFLU_RS12670 0.82 -0.8
2,861,795 - PFLU_RS12670 0.82 +0.6
2,861,795 - PFLU_RS12670 0.82 +0.9
2,861,795 - PFLU_RS12670 0.82 -1.5
2,861,795 - PFLU_RS12670 0.82 +1.0
2,861,874 - PFLU_RS12670 0.85 -0.1
2,861,939 + PFLU_RS12670 0.88 +0.3
2,861,940 - PFLU_RS12670 0.88 -2.2
2,862,174 + -0.9
2,862,174 + +1.2
2,862,175 - -0.2
2,862,175 - +3.1
2,862,175 - -1.0
2,862,175 - -0.1
2,862,233 + -0.7
2,862,233 + -1.0
2,862,234 - -0.1
2,862,234 - -0.3
2,862,472 + PFLU_RS12675 0.49 +0.5
2,862,473 - PFLU_RS12675 0.49 -2.1
2,862,473 - PFLU_RS12675 0.49 +1.7
2,862,533 - PFLU_RS12675 0.65 +1.1
2,862,683 + +1.4
2,862,683 + +0.7
2,862,684 - +0.3
2,862,730 - -0.7
2,862,995 + PFLU_RS12680 0.53 +0.1
2,862,995 + PFLU_RS12680 0.53 +0.2
2,862,995 + PFLU_RS12680 0.53 +0.9
2,862,995 + PFLU_RS12680 0.53 +0.8
2,862,995 + PFLU_RS12680 0.53 -0.9
2,862,996 - PFLU_RS12680 0.53 -0.2
2,862,996 - PFLU_RS12680 0.53 +0.6
2,862,996 - PFLU_RS12680 0.53 -0.2
2,862,996 - PFLU_RS12680 0.53 -0.2
2,863,135 + PFLU_RS12680 0.85 -0.4
2,863,136 - PFLU_RS12680 0.85 +0.8
2,863,219 + -0.8
2,863,223 + +1.1
2,863,223 + -0.7
2,863,224 - +0.8
2,863,278 + PFLU_RS12685 0.14 +1.2
2,863,287 + PFLU_RS12685 0.17 +1.1
2,863,301 + PFLU_RS12685 0.21 +0.1
2,863,301 + PFLU_RS12685 0.21 -1.1
2,863,302 - PFLU_RS12685 0.22 -1.1
2,863,369 + PFLU_RS12685 0.42 -0.2
2,863,370 - PFLU_RS12685 0.42 +0.9
2,863,388 - PFLU_RS12685 0.47 +0.9
2,863,419 + PFLU_RS12685 0.57 +0.2
2,863,420 - PFLU_RS12685 0.57 +0.9
2,863,444 - PFLU_RS12685 0.64 +0.5
2,863,457 - PFLU_RS12685 0.68 +0.6
2,863,457 - PFLU_RS12685 0.68 +0.8
2,863,509 + PFLU_RS12685 0.84 +0.6
2,863,509 + PFLU_RS12685 0.84 -0.7
2,863,509 + PFLU_RS12685 0.84 +0.3
2,863,516 - PFLU_RS12685 0.86 -0.0
2,863,518 + PFLU_RS12685 0.86 +0.5
2,863,533 + +0.9
2,863,533 + -2.1
2,863,538 + +0.2
2,863,554 + -0.3
2,863,583 - -1.4
2,863,603 - +0.2

Or see this region's nucleotide sequence