Strain Fitness in Escherichia coli BW25113 around b4018

Experiment: D-Ribose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntarpA and iclR are separated by 316 nucleotidesiclR and metH are separated by 199 nucleotides b4017: arpA - regulator of acetyl CoA synthetase (NCBI), at 4,218,324 to 4,220,510 arpA b4018: iclR - repressor of aceBA operon (VIMSS), at 4,220,827 to 4,221,651 iclR b4019: metH - B12-dependent methionine synthase (NCBI), at 4,221,851 to 4,225,534 metH Position (kb) 4220 4221 4222Strain fitness (log2 ratio) -3 -2 -1 0 1at 4219.879 kb on + strand, within arpAat 4219.892 kb on + strand, within arpAat 4219.892 kb on + strand, within arpAat 4219.895 kb on + strand, within arpAat 4219.900 kb on - strand, within arpAat 4219.902 kb on + strand, within arpAat 4219.995 kb on + strand, within arpAat 4220.048 kb on + strand, within arpAat 4220.050 kb on + strand, within arpAat 4220.050 kb on + strand, within arpAat 4220.051 kb on + strand, within arpAat 4220.058 kb on + strand, within arpAat 4220.058 kb on + strand, within arpAat 4220.603 kb on - strandat 4220.630 kb on - strandat 4220.728 kb on - strandat 4220.730 kb on - strandat 4220.730 kb on - strandat 4220.759 kb on - strandat 4220.783 kb on - strandat 4220.815 kb on - strandat 4220.815 kb on - strandat 4220.840 kb on - strandat 4220.858 kb on - strandat 4220.872 kb on + strandat 4220.883 kb on + strandat 4220.907 kb on + strandat 4220.960 kb on - strand, within iclRat 4220.969 kb on + strand, within iclRat 4220.971 kb on + strand, within iclRat 4220.992 kb on - strand, within iclRat 4220.992 kb on - strand, within iclRat 4220.994 kb on + strand, within iclRat 4220.997 kb on + strand, within iclRat 4221.000 kb on + strand, within iclRat 4221.001 kb on - strand, within iclRat 4221.013 kb on - strand, within iclRat 4221.064 kb on + strand, within iclRat 4221.089 kb on - strand, within iclRat 4221.094 kb on - strand, within iclRat 4221.094 kb on - strand, within iclRat 4221.099 kb on - strand, within iclRat 4221.100 kb on + strand, within iclRat 4221.100 kb on + strand, within iclRat 4221.100 kb on - strand, within iclRat 4221.127 kb on + strand, within iclRat 4221.140 kb on - strand, within iclRat 4221.140 kb on - strand, within iclRat 4221.186 kb on - strand, within iclRat 4221.227 kb on + strand, within iclRat 4221.227 kb on + strand, within iclRat 4221.232 kb on + strand, within iclRat 4221.234 kb on + strand, within iclRat 4221.234 kb on + strand, within iclRat 4221.235 kb on - strand, within iclRat 4221.235 kb on - strand, within iclRat 4221.277 kb on - strand, within iclRat 4221.277 kb on - strand, within iclRat 4221.320 kb on - strand, within iclRat 4221.330 kb on - strand, within iclRat 4221.335 kb on - strand, within iclRat 4221.452 kb on + strand, within iclRat 4221.524 kb on - strand, within iclRat 4221.585 kb on + strandat 4221.861 kb on + strandat 4221.920 kb on - strandat 4221.922 kb on - strandat 4221.936 kb on + strandat 4221.936 kb on + strandat 4222.014 kb on + strandat 4222.049 kb on + strandat 4222.057 kb on - strandat 4222.068 kb on - strandat 4222.196 kb on + strandat 4222.198 kb on + strandat 4222.215 kb on + strandat 4222.215 kb on + strandat 4222.215 kb on + strandat 4222.270 kb on + strand, within metHat 4222.335 kb on + strand, within metHat 4222.335 kb on + strand, within metHat 4222.389 kb on - strand, within metHat 4222.447 kb on + strand, within metHat 4222.455 kb on - strand, within metHat 4222.455 kb on - strand, within metHat 4222.468 kb on - strand, within metHat 4222.519 kb on + strand, within metHat 4222.549 kb on - strand, within metHat 4222.630 kb on - strand, within metH

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Ribose (C)
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4,219,879 + arpA b4017 0.71 -0.4
4,219,892 + arpA b4017 0.72 -1.3
4,219,892 + arpA b4017 0.72 -1.6
4,219,895 + arpA b4017 0.72 +0.3
4,219,900 - arpA b4017 0.72 -2.9
4,219,902 + arpA b4017 0.72 +0.6
4,219,995 + arpA b4017 0.76 -1.9
4,220,048 + arpA b4017 0.79 -0.4
4,220,050 + arpA b4017 0.79 -1.2
4,220,050 + arpA b4017 0.79 -1.4
4,220,051 + arpA b4017 0.79 -0.7
4,220,058 + arpA b4017 0.79 +0.4
4,220,058 + arpA b4017 0.79 -0.7
4,220,603 - -0.5
4,220,630 - -1.1
4,220,728 - -0.5
4,220,730 - +0.2
4,220,730 - +1.1
4,220,759 - +1.1
4,220,783 - +0.0
4,220,815 - +0.9
4,220,815 - -0.7
4,220,840 - -0.4
4,220,858 - +0.2
4,220,872 + -0.7
4,220,883 + -3.6
4,220,907 + -0.5
4,220,960 - iclR b4018 0.16 +0.3
4,220,969 + iclR b4018 0.17 -2.4
4,220,971 + iclR b4018 0.17 -1.7
4,220,992 - iclR b4018 0.20 +0.8
4,220,992 - iclR b4018 0.20 -1.4
4,220,994 + iclR b4018 0.20 -1.8
4,220,997 + iclR b4018 0.21 -1.7
4,221,000 + iclR b4018 0.21 -3.4
4,221,001 - iclR b4018 0.21 -0.8
4,221,013 - iclR b4018 0.23 -3.0
4,221,064 + iclR b4018 0.29 -0.5
4,221,089 - iclR b4018 0.32 -2.8
4,221,094 - iclR b4018 0.32 -1.5
4,221,094 - iclR b4018 0.32 -1.9
4,221,099 - iclR b4018 0.33 -1.6
4,221,100 + iclR b4018 0.33 +0.8
4,221,100 + iclR b4018 0.33 -0.7
4,221,100 - iclR b4018 0.33 -1.1
4,221,127 + iclR b4018 0.36 -0.3
4,221,140 - iclR b4018 0.38 -2.3
4,221,140 - iclR b4018 0.38 -0.7
4,221,186 - iclR b4018 0.44 -2.7
4,221,227 + iclR b4018 0.48 -1.6
4,221,227 + iclR b4018 0.48 -1.9
4,221,232 + iclR b4018 0.49 -1.5
4,221,234 + iclR b4018 0.49 +0.2
4,221,234 + iclR b4018 0.49 -1.7
4,221,235 - iclR b4018 0.49 -2.1
4,221,235 - iclR b4018 0.49 -1.4
4,221,277 - iclR b4018 0.55 -1.7
4,221,277 - iclR b4018 0.55 -1.2
4,221,320 - iclR b4018 0.60 -1.7
4,221,330 - iclR b4018 0.61 -1.5
4,221,335 - iclR b4018 0.62 -0.8
4,221,452 + iclR b4018 0.76 -0.5
4,221,524 - iclR b4018 0.84 -0.9
4,221,585 + -1.4
4,221,861 + +0.9
4,221,920 - +0.1
4,221,922 - -0.4
4,221,936 + -1.2
4,221,936 + -0.6
4,222,014 + +0.9
4,222,049 + +0.2
4,222,057 - -0.3
4,222,068 - -0.3
4,222,196 + +0.4
4,222,198 + -0.5
4,222,215 + +0.4
4,222,215 + -0.1
4,222,215 + -0.0
4,222,270 + metH b4019 0.11 +0.4
4,222,335 + metH b4019 0.13 -1.1
4,222,335 + metH b4019 0.13 +0.4
4,222,389 - metH b4019 0.15 +0.2
4,222,447 + metH b4019 0.16 +1.3
4,222,455 - metH b4019 0.16 -1.5
4,222,455 - metH b4019 0.16 -0.8
4,222,468 - metH b4019 0.17 -0.3
4,222,519 + metH b4019 0.18 -1.1
4,222,549 - metH b4019 0.19 -0.7
4,222,630 - metH b4019 0.21 +0.1

Or see this region's nucleotide sequence