Experiment: D-Galactose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt galM and galK overlap by 7 nucleotides galK and galT are separated by 3 nucleotides
b0756: galM - galactose-1-epimerase (mutarotase) (NCBI), at 787,020 to 788,060
galM
b0757: galK - galactokinase GalK (EC 2.7.1.6) <small>(from data)</small>, at 788,054 to 789,202
galK
b0758: galT - galactose-1-phosphate uridylyltransferase (NCBI), at 789,206 to 790,252
galT
Position (kb) 788
789
790 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 787.080 kb on - strand at 787.087 kb on + strand at 787.091 kb on - strand at 787.106 kb on + strand at 787.172 kb on - strand, within galM at 787.307 kb on - strand, within galM at 787.308 kb on - strand, within galM at 787.414 kb on - strand, within galM at 787.510 kb on - strand, within galM at 787.569 kb on - strand, within galM at 787.749 kb on + strand, within galM at 787.826 kb on + strand, within galM at 787.876 kb on - strand, within galM at 787.962 kb on - strand at 787.962 kb on - strand at 787.985 kb on + strand at 788.062 kb on + strand at 788.062 kb on + strand at 788.065 kb on - strand at 788.252 kb on + strand, within galK at 788.406 kb on - strand, within galK at 788.406 kb on - strand, within galK at 788.436 kb on - strand, within galK at 788.479 kb on + strand, within galK at 788.479 kb on + strand, within galK at 788.487 kb on - strand, within galK at 788.487 kb on - strand, within galK at 788.518 kb on - strand, within galK at 788.531 kb on + strand, within galK at 788.531 kb on + strand, within galK at 788.531 kb on + strand, within galK at 788.531 kb on + strand, within galK at 788.600 kb on - strand, within galK at 788.610 kb on + strand, within galK at 788.644 kb on - strand, within galK at 788.648 kb on - strand, within galK at 788.732 kb on + strand, within galK at 788.829 kb on + strand, within galK at 788.832 kb on - strand, within galK at 789.031 kb on + strand, within galK at 789.039 kb on - strand, within galK at 789.050 kb on + strand, within galK at 789.063 kb on + strand, within galK at 789.063 kb on + strand, within galK at 789.241 kb on - strand at 789.338 kb on - strand, within galT at 789.338 kb on - strand, within galT at 789.425 kb on - strand, within galT at 789.425 kb on - strand at 789.433 kb on - strand, within galT at 789.581 kb on - strand, within galT at 789.661 kb on + strand, within galT at 789.712 kb on - strand, within galT at 789.712 kb on - strand, within galT at 789.774 kb on - strand, within galT at 789.811 kb on + strand, within galT at 789.908 kb on + strand, within galT at 790.161 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Galactose (C) remove 787,080 - +0.2 787,087 + +0.2 787,091 - -0.2 787,106 + +0.2 787,172 - galM b0756 0.15 +1.5 787,307 - galM b0756 0.28 +0.3 787,308 - galM b0756 0.28 -0.8 787,414 - galM b0756 0.38 -0.7 787,510 - galM b0756 0.47 -0.3 787,569 - galM b0756 0.53 -0.2 787,749 + galM b0756 0.70 -1.2 787,826 + galM b0756 0.77 -0.2 787,876 - galM b0756 0.82 -0.8 787,962 - -0.4 787,962 - +0.2 787,985 + -0.5 788,062 + -3.4 788,062 + -2.5 788,065 - +0.5 788,252 + galK b0757 0.17 -3.5 788,406 - galK b0757 0.31 -1.3 788,406 - galK b0757 0.31 -1.5 788,436 - galK b0757 0.33 -2.8 788,479 + galK b0757 0.37 -3.0 788,479 + galK b0757 0.37 -3.3 788,487 - galK b0757 0.38 -1.7 788,487 - galK b0757 0.38 -1.9 788,518 - galK b0757 0.40 -2.2 788,531 + galK b0757 0.42 -2.0 788,531 + galK b0757 0.42 -4.0 788,531 + galK b0757 0.42 -0.8 788,531 + galK b0757 0.42 -4.6 788,600 - galK b0757 0.48 -4.0 788,610 + galK b0757 0.48 -1.5 788,644 - galK b0757 0.51 -3.0 788,648 - galK b0757 0.52 -3.0 788,732 + galK b0757 0.59 -4.8 788,829 + galK b0757 0.67 -3.6 788,832 - galK b0757 0.68 -2.7 789,031 + galK b0757 0.85 -1.8 789,039 - galK b0757 0.86 -4.5 789,050 + galK b0757 0.87 -3.8 789,063 + galK b0757 0.88 -2.3 789,063 + galK b0757 0.88 -4.0 789,241 - -3.4 789,338 - galT b0758 0.13 -3.6 789,338 - galT b0758 0.13 -2.5 789,425 - galT b0758 0.21 -3.9 789,425 - -1.1 789,433 - galT b0758 0.22 -3.9 789,581 - galT b0758 0.36 -4.6 789,661 + galT b0758 0.43 -4.5 789,712 - galT b0758 0.48 -3.4 789,712 - galT b0758 0.48 -2.6 789,774 - galT b0758 0.54 -4.5 789,811 + galT b0758 0.58 -2.9 789,908 + galT b0758 0.67 -2.6 790,161 - -3.1
Or see this region's nucleotide sequence