Experiment: KB with Acriflavine 1.25 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3302 and Psyr_3303 are separated by 132 nucleotides Psyr_3303 and Psyr_3304 are separated by 17 nucleotides Psyr_3304 and Psyr_3305 overlap by 1 nucleotides
Psyr_3302: Psyr_3302 - mannose-6-phosphate isomerase, type 2 / mannose-1-phosphate guanylyltransferase (GDP), at 3,951,223 to 3,952,680
_3302
Psyr_3303: Psyr_3303 - Polysaccharide export protein, at 3,952,813 to 3,953,592
_3303
Psyr_3304: Psyr_3304 - Lipopolysaccharide biosynthesis, at 3,953,610 to 3,955,601
_3304
Psyr_3305: Psyr_3305 - Glycosyl transferase, group 1, at 3,955,601 to 3,956,779
_3305
Position (kb)
3953
3954
3955
3956 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3952.646 kb on - strand at 3952.646 kb on - strand at 3952.764 kb on + strand at 3952.765 kb on - strand at 3952.784 kb on + strand at 3952.784 kb on + strand at 3952.784 kb on + strand at 3952.785 kb on - strand at 3952.785 kb on - strand at 3952.785 kb on - strand at 3952.785 kb on - strand at 3952.841 kb on + strand at 3952.842 kb on - strand at 3952.864 kb on - strand at 3953.044 kb on + strand, within Psyr_3303 at 3953.044 kb on + strand, within Psyr_3303 at 3953.044 kb on + strand, within Psyr_3303 at 3953.044 kb on + strand, within Psyr_3303 at 3953.044 kb on + strand, within Psyr_3303 at 3953.045 kb on - strand, within Psyr_3303 at 3953.045 kb on - strand, within Psyr_3303 at 3953.045 kb on - strand, within Psyr_3303 at 3953.095 kb on + strand, within Psyr_3303 at 3953.095 kb on + strand, within Psyr_3303 at 3953.095 kb on + strand, within Psyr_3303 at 3953.096 kb on - strand, within Psyr_3303 at 3953.096 kb on - strand, within Psyr_3303 at 3953.096 kb on - strand, within Psyr_3303 at 3953.096 kb on - strand, within Psyr_3303 at 3953.096 kb on - strand, within Psyr_3303 at 3953.098 kb on - strand, within Psyr_3303 at 3953.374 kb on + strand, within Psyr_3303 at 3953.375 kb on - strand, within Psyr_3303 at 3953.383 kb on + strand, within Psyr_3303 at 3953.384 kb on - strand, within Psyr_3303 at 3953.384 kb on - strand, within Psyr_3303 at 3953.384 kb on - strand, within Psyr_3303 at 3953.396 kb on - strand, within Psyr_3303 at 3953.518 kb on + strand at 3953.518 kb on + strand at 3953.519 kb on - strand at 3953.570 kb on - strand at 3953.570 kb on - strand at 3953.570 kb on - strand at 3953.570 kb on - strand at 3953.590 kb on + strand at 3953.590 kb on + strand at 3953.590 kb on + strand at 3953.590 kb on + strand at 3953.590 kb on + strand at 3953.591 kb on - strand at 3953.591 kb on - strand at 3953.908 kb on - strand, within Psyr_3304 at 3953.908 kb on - strand, within Psyr_3304 at 3954.237 kb on + strand, within Psyr_3304 at 3954.237 kb on + strand, within Psyr_3304 at 3954.238 kb on - strand, within Psyr_3304 at 3954.279 kb on + strand, within Psyr_3304 at 3954.280 kb on - strand, within Psyr_3304 at 3954.300 kb on + strand, within Psyr_3304 at 3954.326 kb on + strand, within Psyr_3304 at 3954.326 kb on + strand, within Psyr_3304 at 3954.326 kb on + strand, within Psyr_3304 at 3954.327 kb on - strand, within Psyr_3304 at 3954.327 kb on - strand, within Psyr_3304 at 3954.327 kb on - strand, within Psyr_3304 at 3954.327 kb on - strand, within Psyr_3304 at 3954.649 kb on + strand, within Psyr_3304 at 3954.650 kb on - strand, within Psyr_3304 at 3954.650 kb on - strand, within Psyr_3304 at 3954.684 kb on + strand, within Psyr_3304 at 3954.684 kb on + strand, within Psyr_3304 at 3954.861 kb on + strand, within Psyr_3304 at 3954.861 kb on + strand, within Psyr_3304 at 3954.870 kb on + strand, within Psyr_3304 at 3954.930 kb on - strand, within Psyr_3304 at 3954.930 kb on - strand, within Psyr_3304 at 3954.932 kb on + strand, within Psyr_3304 at 3955.044 kb on + strand, within Psyr_3304 at 3955.045 kb on - strand, within Psyr_3304 at 3955.053 kb on + strand, within Psyr_3304 at 3955.189 kb on - strand, within Psyr_3304 at 3955.213 kb on + strand, within Psyr_3304 at 3955.213 kb on + strand, within Psyr_3304 at 3955.213 kb on + strand, within Psyr_3304 at 3955.241 kb on + strand, within Psyr_3304 at 3955.241 kb on + strand, within Psyr_3304 at 3955.608 kb on + strand at 3955.705 kb on + strand at 3955.706 kb on - strand at 3956.180 kb on + strand, within Psyr_3305 at 3956.180 kb on + strand, within Psyr_3305 at 3956.180 kb on + strand, within Psyr_3305 at 3956.333 kb on + strand, within Psyr_3305 at 3956.334 kb on - strand, within Psyr_3305 at 3956.334 kb on - strand, within Psyr_3305 at 3956.548 kb on - strand, within Psyr_3305
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acriflavine 1.25 ug/ml remove 3,952,646 - -0.7 3,952,646 - -0.2 3,952,764 + +0.3 3,952,765 - -0.4 3,952,784 + +1.9 3,952,784 + +0.1 3,952,784 + -0.7 3,952,785 - +0.7 3,952,785 - -0.6 3,952,785 - +1.9 3,952,785 - -0.3 3,952,841 + +0.4 3,952,842 - -1.4 3,952,864 - -1.7 3,953,044 + Psyr_3303 0.30 -0.5 3,953,044 + Psyr_3303 0.30 -0.6 3,953,044 + Psyr_3303 0.30 +0.1 3,953,044 + Psyr_3303 0.30 +0.9 3,953,044 + Psyr_3303 0.30 +0.4 3,953,045 - Psyr_3303 0.30 -0.0 3,953,045 - Psyr_3303 0.30 -0.7 3,953,045 - Psyr_3303 0.30 +1.5 3,953,095 + Psyr_3303 0.36 +0.7 3,953,095 + Psyr_3303 0.36 +0.6 3,953,095 + Psyr_3303 0.36 +0.9 3,953,096 - Psyr_3303 0.36 +0.6 3,953,096 - Psyr_3303 0.36 +0.1 3,953,096 - Psyr_3303 0.36 +0.7 3,953,096 - Psyr_3303 0.36 -0.3 3,953,096 - Psyr_3303 0.36 -0.4 3,953,098 - Psyr_3303 0.37 +0.3 3,953,374 + Psyr_3303 0.72 +0.3 3,953,375 - Psyr_3303 0.72 +0.1 3,953,383 + Psyr_3303 0.73 +1.1 3,953,384 - Psyr_3303 0.73 -0.9 3,953,384 - Psyr_3303 0.73 -1.0 3,953,384 - Psyr_3303 0.73 +0.5 3,953,396 - Psyr_3303 0.75 +0.6 3,953,518 + -1.9 3,953,518 + +0.9 3,953,519 - -0.0 3,953,570 - +0.1 3,953,570 - +0.4 3,953,570 - -0.3 3,953,570 - -0.0 3,953,590 + +0.5 3,953,590 + -1.4 3,953,590 + +0.3 3,953,590 + -0.1 3,953,590 + +0.5 3,953,591 - +0.6 3,953,591 - +0.1 3,953,908 - Psyr_3304 0.15 +1.5 3,953,908 - Psyr_3304 0.15 -0.0 3,954,237 + Psyr_3304 0.31 +0.2 3,954,237 + Psyr_3304 0.31 +0.1 3,954,238 - Psyr_3304 0.32 +0.1 3,954,279 + Psyr_3304 0.34 -0.9 3,954,280 - Psyr_3304 0.34 -0.7 3,954,300 + Psyr_3304 0.35 +0.2 3,954,326 + Psyr_3304 0.36 -1.4 3,954,326 + Psyr_3304 0.36 +4.2 3,954,326 + Psyr_3304 0.36 -0.5 3,954,327 - Psyr_3304 0.36 -0.6 3,954,327 - Psyr_3304 0.36 +1.5 3,954,327 - Psyr_3304 0.36 -0.9 3,954,327 - Psyr_3304 0.36 -0.0 3,954,649 + Psyr_3304 0.52 -0.6 3,954,650 - Psyr_3304 0.52 +0.6 3,954,650 - Psyr_3304 0.52 -0.9 3,954,684 + Psyr_3304 0.54 -0.9 3,954,684 + Psyr_3304 0.54 -0.6 3,954,861 + Psyr_3304 0.63 -0.7 3,954,861 + Psyr_3304 0.63 +0.1 3,954,870 + Psyr_3304 0.63 -0.7 3,954,930 - Psyr_3304 0.66 -0.7 3,954,930 - Psyr_3304 0.66 -0.3 3,954,932 + Psyr_3304 0.66 -0.5 3,955,044 + Psyr_3304 0.72 -0.1 3,955,045 - Psyr_3304 0.72 -0.1 3,955,053 + Psyr_3304 0.72 +0.1 3,955,189 - Psyr_3304 0.79 -0.7 3,955,213 + Psyr_3304 0.80 +0.3 3,955,213 + Psyr_3304 0.80 -2.3 3,955,213 + Psyr_3304 0.80 -1.0 3,955,241 + Psyr_3304 0.82 -0.3 3,955,241 + Psyr_3304 0.82 -0.2 3,955,608 + -0.4 3,955,705 + +1.2 3,955,706 - +1.3 3,956,180 + Psyr_3305 0.49 -0.3 3,956,180 + Psyr_3305 0.49 +0.3 3,956,180 + Psyr_3305 0.49 +0.2 3,956,333 + Psyr_3305 0.62 -0.1 3,956,334 - Psyr_3305 0.62 -2.4 3,956,334 - Psyr_3305 0.62 +0.6 3,956,548 - Psyr_3305 0.80 -0.1
Or see this region's nucleotide sequence