Experiment: KB with Acriflavine 1.25 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1970 and Psyr_1971 are separated by 186 nucleotides Psyr_1971 and Psyr_1972 are separated by 52 nucleotides Psyr_1972 and Psyr_1973 are separated by 271 nucleotides
Psyr_1970: Psyr_1970 - Secretion protein HlyD, at 2,284,475 to 2,285,647
_1970
Psyr_1971: Psyr_1971 - Peptidase S45, penicillin amidase, at 2,285,834 to 2,288,173
_1971
Psyr_1972: Psyr_1972 - conserved hypothetical protein, at 2,288,226 to 2,288,537
_1972
Psyr_1973: Psyr_1973 - Aspartate transaminase, at 2,288,809 to 2,290,005
_1973
Position (kb)
2285
2286
2287
2288
2289 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2284.918 kb on - strand, within Psyr_1970 at 2285.093 kb on - strand, within Psyr_1970 at 2285.332 kb on - strand, within Psyr_1970 at 2285.802 kb on - strand at 2285.840 kb on - strand at 2285.840 kb on - strand at 2286.089 kb on + strand, within Psyr_1971 at 2286.090 kb on - strand, within Psyr_1971 at 2286.231 kb on - strand, within Psyr_1971 at 2286.519 kb on + strand, within Psyr_1971 at 2286.520 kb on - strand, within Psyr_1971 at 2286.599 kb on + strand, within Psyr_1971 at 2286.872 kb on + strand, within Psyr_1971 at 2286.928 kb on + strand, within Psyr_1971 at 2286.928 kb on + strand, within Psyr_1971 at 2286.928 kb on + strand, within Psyr_1971 at 2286.928 kb on + strand, within Psyr_1971 at 2286.928 kb on + strand, within Psyr_1971 at 2286.928 kb on + strand, within Psyr_1971 at 2286.929 kb on - strand, within Psyr_1971 at 2286.929 kb on - strand, within Psyr_1971 at 2286.929 kb on - strand, within Psyr_1971 at 2286.990 kb on - strand, within Psyr_1971 at 2287.039 kb on + strand, within Psyr_1971 at 2287.039 kb on + strand, within Psyr_1971 at 2287.039 kb on + strand, within Psyr_1971 at 2287.039 kb on + strand, within Psyr_1971 at 2287.040 kb on - strand, within Psyr_1971 at 2287.040 kb on - strand, within Psyr_1971 at 2287.100 kb on + strand, within Psyr_1971 at 2287.100 kb on + strand, within Psyr_1971 at 2287.101 kb on - strand, within Psyr_1971 at 2287.250 kb on + strand, within Psyr_1971 at 2287.250 kb on + strand, within Psyr_1971 at 2287.487 kb on + strand, within Psyr_1971 at 2287.632 kb on - strand, within Psyr_1971 at 2287.819 kb on + strand, within Psyr_1971 at 2287.819 kb on + strand, within Psyr_1971 at 2287.820 kb on - strand, within Psyr_1971 at 2287.961 kb on + strand at 2287.962 kb on - strand at 2288.135 kb on + strand at 2288.240 kb on + strand at 2288.283 kb on + strand, within Psyr_1972 at 2288.324 kb on - strand, within Psyr_1972 at 2288.324 kb on - strand, within Psyr_1972 at 2288.541 kb on + strand at 2288.542 kb on - strand at 2288.542 kb on - strand at 2288.657 kb on - strand at 2288.657 kb on - strand at 2288.657 kb on - strand at 2288.657 kb on - strand at 2288.671 kb on - strand at 2288.677 kb on + strand at 2288.677 kb on + strand at 2288.678 kb on - strand at 2288.678 kb on - strand at 2288.684 kb on + strand at 2288.684 kb on + strand at 2288.684 kb on + strand at 2288.684 kb on + strand at 2288.684 kb on + strand at 2288.684 kb on + strand at 2288.691 kb on + strand at 2288.698 kb on + strand at 2288.698 kb on + strand at 2288.705 kb on + strand at 2288.712 kb on + strand at 2288.712 kb on + strand at 2288.712 kb on + strand at 2288.712 kb on + strand at 2288.712 kb on + strand at 2288.810 kb on + strand at 2288.839 kb on + strand at 2288.840 kb on - strand at 2288.840 kb on - strand at 2288.840 kb on - strand at 2288.878 kb on - strand at 2288.878 kb on - strand at 2288.878 kb on - strand at 2288.897 kb on + strand at 2288.897 kb on + strand at 2288.897 kb on + strand at 2288.897 kb on + strand at 2289.152 kb on + strand, within Psyr_1973 at 2289.153 kb on - strand, within Psyr_1973
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acriflavine 1.25 ug/ml remove 2,284,918 - Psyr_1970 0.38 +1.6 2,285,093 - Psyr_1970 0.53 -1.4 2,285,332 - Psyr_1970 0.73 -0.9 2,285,802 - -3.1 2,285,840 - -0.0 2,285,840 - +0.3 2,286,089 + Psyr_1971 0.11 +1.0 2,286,090 - Psyr_1971 0.11 -0.0 2,286,231 - Psyr_1971 0.17 -0.2 2,286,519 + Psyr_1971 0.29 +0.5 2,286,520 - Psyr_1971 0.29 -0.6 2,286,599 + Psyr_1971 0.33 -0.3 2,286,872 + Psyr_1971 0.44 -0.1 2,286,928 + Psyr_1971 0.47 +0.4 2,286,928 + Psyr_1971 0.47 -1.5 2,286,928 + Psyr_1971 0.47 +0.4 2,286,928 + Psyr_1971 0.47 +1.1 2,286,928 + Psyr_1971 0.47 +0.4 2,286,928 + Psyr_1971 0.47 +0.7 2,286,929 - Psyr_1971 0.47 -0.0 2,286,929 - Psyr_1971 0.47 -0.0 2,286,929 - Psyr_1971 0.47 +0.7 2,286,990 - Psyr_1971 0.49 -0.9 2,287,039 + Psyr_1971 0.51 -0.5 2,287,039 + Psyr_1971 0.51 -0.5 2,287,039 + Psyr_1971 0.51 +2.6 2,287,039 + Psyr_1971 0.51 +0.4 2,287,040 - Psyr_1971 0.52 -2.2 2,287,040 - Psyr_1971 0.52 -0.6 2,287,100 + Psyr_1971 0.54 +0.4 2,287,100 + Psyr_1971 0.54 +2.3 2,287,101 - Psyr_1971 0.54 -1.0 2,287,250 + Psyr_1971 0.61 -1.4 2,287,250 + Psyr_1971 0.61 -0.1 2,287,487 + Psyr_1971 0.71 +1.3 2,287,632 - Psyr_1971 0.77 +0.2 2,287,819 + Psyr_1971 0.85 -0.0 2,287,819 + Psyr_1971 0.85 -0.7 2,287,820 - Psyr_1971 0.85 +0.0 2,287,961 + -1.4 2,287,962 - -0.0 2,288,135 + +0.8 2,288,240 + +0.2 2,288,283 + Psyr_1972 0.18 -0.6 2,288,324 - Psyr_1972 0.31 -0.3 2,288,324 - Psyr_1972 0.31 -0.5 2,288,541 + +0.2 2,288,542 - +0.3 2,288,542 - -0.3 2,288,657 - -1.2 2,288,657 - +1.6 2,288,657 - -1.2 2,288,657 - -1.2 2,288,671 - +0.2 2,288,677 + -0.6 2,288,677 + +1.4 2,288,678 - -0.9 2,288,678 - -0.0 2,288,684 + +0.2 2,288,684 + -0.2 2,288,684 + +0.1 2,288,684 + +1.5 2,288,684 + -0.6 2,288,684 + +1.0 2,288,691 + -1.6 2,288,698 + +0.2 2,288,698 + +0.1 2,288,705 + +0.1 2,288,712 + -0.7 2,288,712 + +0.3 2,288,712 + -0.3 2,288,712 + -1.5 2,288,712 + +0.6 2,288,810 + -0.7 2,288,839 + +1.0 2,288,840 - +0.5 2,288,840 - +0.6 2,288,840 - -1.6 2,288,878 - +1.0 2,288,878 - -1.4 2,288,878 - +1.0 2,288,897 + -1.9 2,288,897 + -1.2 2,288,897 + -1.4 2,288,897 + +1.5 2,289,152 + Psyr_1973 0.29 -0.6 2,289,153 - Psyr_1973 0.29 -0.4
Or see this region's nucleotide sequence