Experiment: KB with Acriflavine 1.25 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1614 and Psyr_1615 are separated by 32 nucleotides Psyr_1615 and Psyr_1616 are separated by 41 nucleotides Psyr_1616 and Psyr_1617 overlap by 17 nucleotides
Psyr_1614: Psyr_1614 - lipid A biosynthesis acyltransferase, at 1,816,749 to 1,817,681
_1614
Psyr_1615: Psyr_1615 - Patatin, at 1,817,714 to 1,818,898
_1615
Psyr_1616: Psyr_1616 - Rieske [2Fe-2S] region, at 1,818,940 to 1,819,176
_1616
Psyr_1617: Psyr_1617 - conserved hypothetical protein, at 1,819,160 to 1,820,017
_1617
Position (kb)
1817
1818
1819 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1816.766 kb on + strand at 1816.766 kb on + strand at 1816.890 kb on + strand, within Psyr_1614 at 1816.890 kb on + strand, within Psyr_1614 at 1816.890 kb on + strand, within Psyr_1614 at 1816.891 kb on - strand, within Psyr_1614 at 1817.202 kb on + strand, within Psyr_1614 at 1817.202 kb on + strand, within Psyr_1614 at 1817.203 kb on - strand, within Psyr_1614 at 1817.315 kb on - strand, within Psyr_1614 at 1817.315 kb on - strand, within Psyr_1614 at 1817.358 kb on + strand, within Psyr_1614 at 1817.525 kb on + strand, within Psyr_1614 at 1817.664 kb on + strand at 1817.664 kb on + strand at 1817.664 kb on + strand at 1817.665 kb on - strand at 1817.665 kb on - strand at 1817.665 kb on - strand at 1817.665 kb on - strand at 1817.675 kb on + strand at 1817.675 kb on + strand at 1817.675 kb on + strand at 1817.675 kb on + strand at 1817.676 kb on - strand at 1817.676 kb on - strand at 1817.679 kb on + strand at 1817.680 kb on - strand at 1817.680 kb on - strand at 1817.680 kb on - strand at 1817.680 kb on - strand at 1817.711 kb on + strand at 1817.711 kb on + strand at 1817.711 kb on + strand at 1817.711 kb on + strand at 1817.712 kb on - strand at 1817.712 kb on - strand at 1817.712 kb on - strand at 1817.712 kb on - strand at 1817.712 kb on - strand at 1817.712 kb on - strand at 1817.808 kb on - strand at 1817.997 kb on + strand, within Psyr_1615 at 1818.259 kb on - strand, within Psyr_1615 at 1818.259 kb on - strand, within Psyr_1615 at 1818.362 kb on + strand, within Psyr_1615 at 1818.362 kb on + strand, within Psyr_1615 at 1818.363 kb on - strand, within Psyr_1615 at 1818.363 kb on - strand, within Psyr_1615 at 1818.363 kb on - strand, within Psyr_1615 at 1818.417 kb on + strand, within Psyr_1615 at 1818.417 kb on + strand, within Psyr_1615 at 1818.470 kb on - strand, within Psyr_1615 at 1818.562 kb on + strand, within Psyr_1615 at 1818.794 kb on + strand at 1818.794 kb on + strand at 1818.794 kb on + strand at 1818.794 kb on + strand at 1818.795 kb on - strand at 1818.795 kb on - strand at 1818.941 kb on + strand at 1818.941 kb on + strand at 1818.942 kb on - strand at 1819.112 kb on + strand, within Psyr_1616 at 1819.132 kb on + strand, within Psyr_1616 at 1819.374 kb on + strand, within Psyr_1617 at 1819.374 kb on + strand, within Psyr_1617 at 1819.374 kb on + strand, within Psyr_1617 at 1819.375 kb on - strand, within Psyr_1617 at 1819.375 kb on - strand, within Psyr_1617 at 1819.377 kb on + strand, within Psyr_1617 at 1819.377 kb on + strand, within Psyr_1617 at 1819.377 kb on + strand, within Psyr_1617 at 1819.377 kb on + strand, within Psyr_1617 at 1819.378 kb on - strand, within Psyr_1617 at 1819.378 kb on - strand, within Psyr_1617 at 1819.378 kb on - strand, within Psyr_1617 at 1819.378 kb on - strand, within Psyr_1617 at 1819.419 kb on + strand, within Psyr_1617 at 1819.420 kb on - strand, within Psyr_1617 at 1819.420 kb on - strand, within Psyr_1617 at 1819.455 kb on + strand, within Psyr_1617 at 1819.455 kb on + strand, within Psyr_1617 at 1819.455 kb on + strand, within Psyr_1617 at 1819.455 kb on + strand, within Psyr_1617 at 1819.456 kb on - strand, within Psyr_1617 at 1819.456 kb on - strand, within Psyr_1617 at 1819.516 kb on + strand, within Psyr_1617 at 1819.516 kb on + strand, within Psyr_1617 at 1819.516 kb on + strand, within Psyr_1617 at 1819.516 kb on + strand, within Psyr_1617 at 1819.516 kb on + strand, within Psyr_1617 at 1819.517 kb on - strand, within Psyr_1617 at 1819.517 kb on - strand, within Psyr_1617 at 1819.517 kb on - strand, within Psyr_1617 at 1819.554 kb on - strand, within Psyr_1617 at 1819.558 kb on - strand, within Psyr_1617 at 1819.605 kb on + strand, within Psyr_1617 at 1819.615 kb on + strand, within Psyr_1617 at 1819.710 kb on + strand, within Psyr_1617 at 1819.710 kb on + strand, within Psyr_1617 at 1819.710 kb on + strand, within Psyr_1617 at 1819.711 kb on - strand, within Psyr_1617 at 1819.711 kb on - strand, within Psyr_1617 at 1819.797 kb on + strand, within Psyr_1617 at 1819.797 kb on + strand, within Psyr_1617 at 1819.797 kb on + strand, within Psyr_1617 at 1819.798 kb on - strand, within Psyr_1617 at 1819.798 kb on - strand, within Psyr_1617 at 1819.798 kb on - strand, within Psyr_1617 at 1819.798 kb on - strand, within Psyr_1617 at 1819.798 kb on - strand, within Psyr_1617 at 1819.798 kb on - strand, within Psyr_1617 at 1819.798 kb on - strand, within Psyr_1617
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acriflavine 1.25 ug/ml remove 1,816,766 + -2.5 1,816,766 + -1.3 1,816,890 + Psyr_1614 0.15 -1.8 1,816,890 + Psyr_1614 0.15 -2.3 1,816,890 + Psyr_1614 0.15 -3.2 1,816,891 - Psyr_1614 0.15 -2.3 1,817,202 + Psyr_1614 0.49 -1.6 1,817,202 + Psyr_1614 0.49 -3.3 1,817,203 - Psyr_1614 0.49 -1.3 1,817,315 - Psyr_1614 0.61 -0.6 1,817,315 - Psyr_1614 0.61 -2.5 1,817,358 + Psyr_1614 0.65 -2.0 1,817,525 + Psyr_1614 0.83 -2.5 1,817,664 + +0.0 1,817,664 + -1.0 1,817,664 + -1.4 1,817,665 - -2.6 1,817,665 - -2.7 1,817,665 - -2.7 1,817,665 - -3.6 1,817,675 + -1.2 1,817,675 + -2.2 1,817,675 + -0.1 1,817,675 + -1.3 1,817,676 - -1.9 1,817,676 - -0.7 1,817,679 + -0.1 1,817,680 - -1.3 1,817,680 - -1.0 1,817,680 - +1.4 1,817,680 - -0.4 1,817,711 + +0.8 1,817,711 + -0.1 1,817,711 + -1.9 1,817,711 + -1.1 1,817,712 - -0.4 1,817,712 - -0.4 1,817,712 - +1.0 1,817,712 - +1.4 1,817,712 - -0.1 1,817,712 - -1.3 1,817,808 - +0.9 1,817,997 + Psyr_1615 0.24 +1.2 1,818,259 - Psyr_1615 0.46 +0.0 1,818,259 - Psyr_1615 0.46 -1.0 1,818,362 + Psyr_1615 0.55 +0.8 1,818,362 + Psyr_1615 0.55 +0.0 1,818,363 - Psyr_1615 0.55 +1.4 1,818,363 - Psyr_1615 0.55 -1.1 1,818,363 - Psyr_1615 0.55 -0.0 1,818,417 + Psyr_1615 0.59 -0.7 1,818,417 + Psyr_1615 0.59 +0.0 1,818,470 - Psyr_1615 0.64 +0.7 1,818,562 + Psyr_1615 0.72 +0.7 1,818,794 + +1.6 1,818,794 + -0.5 1,818,794 + +0.0 1,818,794 + +0.2 1,818,795 - -0.4 1,818,795 - +1.0 1,818,941 + -1.9 1,818,941 + -0.1 1,818,942 - +1.3 1,819,112 + Psyr_1616 0.73 +0.0 1,819,132 + Psyr_1616 0.81 -0.5 1,819,374 + Psyr_1617 0.25 -0.4 1,819,374 + Psyr_1617 0.25 +0.8 1,819,374 + Psyr_1617 0.25 -0.1 1,819,375 - Psyr_1617 0.25 -0.5 1,819,375 - Psyr_1617 0.25 +0.5 1,819,377 + Psyr_1617 0.25 +0.2 1,819,377 + Psyr_1617 0.25 -0.3 1,819,377 + Psyr_1617 0.25 -0.6 1,819,377 + Psyr_1617 0.25 -2.3 1,819,378 - Psyr_1617 0.25 +0.2 1,819,378 - Psyr_1617 0.25 +1.0 1,819,378 - Psyr_1617 0.25 -0.6 1,819,378 - Psyr_1617 0.25 +1.7 1,819,419 + Psyr_1617 0.30 -0.3 1,819,420 - Psyr_1617 0.30 +0.9 1,819,420 - Psyr_1617 0.30 +0.3 1,819,455 + Psyr_1617 0.34 -0.4 1,819,455 + Psyr_1617 0.34 +1.3 1,819,455 + Psyr_1617 0.34 +0.0 1,819,455 + Psyr_1617 0.34 +0.5 1,819,456 - Psyr_1617 0.34 +1.0 1,819,456 - Psyr_1617 0.34 +0.3 1,819,516 + Psyr_1617 0.41 +0.1 1,819,516 + Psyr_1617 0.41 +0.0 1,819,516 + Psyr_1617 0.41 +1.2 1,819,516 + Psyr_1617 0.41 +0.6 1,819,516 + Psyr_1617 0.41 +0.5 1,819,517 - Psyr_1617 0.42 +0.1 1,819,517 - Psyr_1617 0.42 -2.3 1,819,517 - Psyr_1617 0.42 -0.6 1,819,554 - Psyr_1617 0.46 -2.2 1,819,558 - Psyr_1617 0.46 +0.9 1,819,605 + Psyr_1617 0.52 -2.2 1,819,615 + Psyr_1617 0.53 -0.3 1,819,710 + Psyr_1617 0.64 -1.4 1,819,710 + Psyr_1617 0.64 -0.8 1,819,710 + Psyr_1617 0.64 -1.0 1,819,711 - Psyr_1617 0.64 +0.7 1,819,711 - Psyr_1617 0.64 -0.5 1,819,797 + Psyr_1617 0.74 +0.2 1,819,797 + Psyr_1617 0.74 +0.3 1,819,797 + Psyr_1617 0.74 -0.1 1,819,798 - Psyr_1617 0.74 +0.4 1,819,798 - Psyr_1617 0.74 -0.7 1,819,798 - Psyr_1617 0.74 -0.2 1,819,798 - Psyr_1617 0.74 -0.1 1,819,798 - Psyr_1617 0.74 -0.1 1,819,798 - Psyr_1617 0.74 +0.3 1,819,798 - Psyr_1617 0.74 -0.1
Or see this region's nucleotide sequence