Experiment: KB with Acriflavine 1.25 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1610 and Psyr_1611 are separated by 90 nucleotides Psyr_1611 and Psyr_1612 overlap by 1 nucleotides Psyr_1612 and Psyr_1613 are separated by 231 nucleotides
Psyr_1610: Psyr_1610 - ribosomal large subunit pseudouridine synthase A, at 1,813,864 to 1,814,499
_1610
Psyr_1611: Psyr_1611 - cell division topological specificity factor MinE, at 1,814,590 to 1,814,844
_1611
Psyr_1612: Psyr_1612 - septum site-determining protein MinD, at 1,814,844 to 1,815,656
_1612
Psyr_1613: Psyr_1613 - septum site-determining protein MinC, at 1,815,888 to 1,816,628
_1613
Position (kb) 1814
1815
1816 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1813.982 kb on + strand, within Psyr_1610 at 1814.078 kb on + strand, within Psyr_1610 at 1814.078 kb on + strand, within Psyr_1610 at 1814.078 kb on + strand, within Psyr_1610 at 1814.079 kb on - strand, within Psyr_1610 at 1814.079 kb on - strand, within Psyr_1610 at 1814.079 kb on - strand, within Psyr_1610 at 1814.079 kb on - strand, within Psyr_1610 at 1814.079 kb on - strand, within Psyr_1610 at 1814.079 kb on - strand, within Psyr_1610 at 1814.079 kb on - strand, within Psyr_1610 at 1814.079 kb on - strand, within Psyr_1610 at 1814.234 kb on + strand, within Psyr_1610 at 1814.234 kb on + strand, within Psyr_1610 at 1814.234 kb on + strand, within Psyr_1610 at 1814.518 kb on + strand at 1814.883 kb on - strand at 1814.944 kb on + strand, within Psyr_1612 at 1815.010 kb on - strand, within Psyr_1612 at 1815.113 kb on - strand, within Psyr_1612 at 1815.259 kb on + strand, within Psyr_1612 at 1815.259 kb on + strand, within Psyr_1612 at 1815.259 kb on + strand, within Psyr_1612 at 1815.259 kb on + strand, within Psyr_1612 at 1815.260 kb on - strand, within Psyr_1612 at 1815.260 kb on - strand, within Psyr_1612 at 1815.314 kb on - strand, within Psyr_1612 at 1815.764 kb on - strand at 1815.775 kb on - strand at 1815.790 kb on + strand at 1815.791 kb on - strand at 1815.791 kb on - strand at 1815.791 kb on - strand at 1815.791 kb on - strand at 1815.889 kb on + strand at 1815.982 kb on + strand, within Psyr_1613 at 1815.982 kb on + strand, within Psyr_1613 at 1815.983 kb on - strand, within Psyr_1613 at 1815.983 kb on - strand, within Psyr_1613 at 1816.168 kb on + strand, within Psyr_1613 at 1816.169 kb on - strand, within Psyr_1613 at 1816.169 kb on - strand, within Psyr_1613 at 1816.473 kb on - strand, within Psyr_1613 at 1816.636 kb on - strand at 1816.637 kb on + strand at 1816.637 kb on + strand at 1816.637 kb on + strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acriflavine 1.25 ug/ml remove 1,813,982 + Psyr_1610 0.19 +0.4 1,814,078 + Psyr_1610 0.34 +0.6 1,814,078 + Psyr_1610 0.34 +1.0 1,814,078 + Psyr_1610 0.34 +0.0 1,814,079 - Psyr_1610 0.34 +0.4 1,814,079 - Psyr_1610 0.34 +1.6 1,814,079 - Psyr_1610 0.34 +1.7 1,814,079 - Psyr_1610 0.34 +0.9 1,814,079 - Psyr_1610 0.34 +0.2 1,814,079 - Psyr_1610 0.34 +0.5 1,814,079 - Psyr_1610 0.34 +2.4 1,814,079 - Psyr_1610 0.34 +1.6 1,814,234 + Psyr_1610 0.58 -0.8 1,814,234 + Psyr_1610 0.58 +1.0 1,814,234 + Psyr_1610 0.58 +0.4 1,814,518 + -0.3 1,814,883 - -2.0 1,814,944 + Psyr_1612 0.12 -1.3 1,815,010 - Psyr_1612 0.20 -0.7 1,815,113 - Psyr_1612 0.33 -0.6 1,815,259 + Psyr_1612 0.51 -2.0 1,815,259 + Psyr_1612 0.51 -1.5 1,815,259 + Psyr_1612 0.51 -2.0 1,815,259 + Psyr_1612 0.51 -1.0 1,815,260 - Psyr_1612 0.51 -1.3 1,815,260 - Psyr_1612 0.51 -2.0 1,815,314 - Psyr_1612 0.58 -0.6 1,815,764 - -1.8 1,815,775 - +0.5 1,815,790 + -0.6 1,815,791 - +0.0 1,815,791 - -2.1 1,815,791 - +0.2 1,815,791 - -0.8 1,815,889 + -2.0 1,815,982 + Psyr_1613 0.13 -1.3 1,815,982 + Psyr_1613 0.13 -1.0 1,815,983 - Psyr_1613 0.13 -0.6 1,815,983 - Psyr_1613 0.13 -0.6 1,816,168 + Psyr_1613 0.38 -1.0 1,816,169 - Psyr_1613 0.38 -2.8 1,816,169 - Psyr_1613 0.38 +0.0 1,816,473 - Psyr_1613 0.79 -1.6 1,816,636 - +0.0 1,816,637 + -1.6 1,816,637 + -1.6 1,816,637 + +0.0 1,816,638 - -1.5 1,816,638 - -0.7 1,816,638 - -0.3 1,816,638 - -0.7 1,816,638 - -0.4 1,816,638 - -1.6 1,816,638 - -0.2 1,816,638 - -0.3 1,816,638 - -1.0 1,816,638 - +0.0 1,816,638 - -1.5 1,816,638 - -0.6 1,816,638 - -1.8 1,816,638 - +0.0
Or see this region's nucleotide sequence