Experiment: KB with Acriflavine 1.25 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0209 and Psyr_0210 are separated by 42 nucleotides Psyr_0210 and Psyr_0211 are separated by 147 nucleotides Psyr_0211 and Psyr_0212 are separated by 13 nucleotides
Psyr_0209: Psyr_0209 - RelA/SpoT protein, at 223,004 to 225,127
_0209
Psyr_0210: Psyr_0210 - DNA-directed RNA polymerase subunit omega, at 225,170 to 225,433
_0210
Psyr_0211: Psyr_0211 - guanylate kinase, at 225,581 to 226,225
_0211
Psyr_0212: Psyr_0212 - Conserved hypothetical protein 255, at 226,239 to 227,102
_0212
Position (kb) 225
226
227 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 225.151 kb on - strand at 225.151 kb on - strand at 225.151 kb on - strand at 225.151 kb on - strand at 225.151 kb on - strand at 225.151 kb on - strand at 225.166 kb on - strand at 225.166 kb on - strand at 225.253 kb on - strand, within Psyr_0210 at 225.405 kb on - strand, within Psyr_0210 at 225.438 kb on - strand at 225.497 kb on - strand at 225.505 kb on - strand at 225.505 kb on - strand at 225.613 kb on - strand at 225.613 kb on - strand at 226.235 kb on - strand at 226.241 kb on - strand at 226.248 kb on - strand at 226.341 kb on - strand, within Psyr_0212 at 226.341 kb on - strand, within Psyr_0212 at 226.341 kb on - strand, within Psyr_0212 at 226.341 kb on - strand, within Psyr_0212 at 226.341 kb on - strand, within Psyr_0212 at 227.071 kb on - strand at 227.110 kb on - strand at 227.110 kb on - strand at 227.196 kb on - strand at 227.212 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acriflavine 1.25 ug/ml remove 225,151 - -2.2 225,151 - -1.2 225,151 - -0.3 225,151 - +0.1 225,151 - +1.0 225,151 - +1.3 225,166 - -2.8 225,166 - -1.0 225,253 - Psyr_0210 0.31 +0.6 225,405 - Psyr_0210 0.89 -1.6 225,438 - -0.2 225,497 - +1.0 225,505 - -1.2 225,505 - -1.6 225,613 - -1.4 225,613 - -0.2 226,235 - -1.0 226,241 - -1.6 226,248 - +0.6 226,341 - Psyr_0212 0.12 -0.4 226,341 - Psyr_0212 0.12 +2.3 226,341 - Psyr_0212 0.12 -2.0 226,341 - Psyr_0212 0.12 -0.3 226,341 - Psyr_0212 0.12 -3.0 227,071 - +1.4 227,110 - -1.1 227,110 - -1.4 227,196 - +0.2 227,212 - +0.3
Or see this region's nucleotide sequence