Experiment: KB with Acriflavine 1.25 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0090 and Psyr_0091 are separated by 179 nucleotides Psyr_0091 and Psyr_0092 are separated by 286 nucleotides Psyr_0092 and Psyr_0093 are separated by 45 nucleotides
Psyr_0090: Psyr_0090 - 4-aminobutyrate aminotransferase apoenzyme, at 98,488 to 99,768
_0090
Psyr_0091: Psyr_0091 - succinate semialdehyde dehydrogenase, at 99,948 to 101,390
_0091
Psyr_0092: Psyr_0092 - Lysine exporter protein (LYSE/YGGA), at 101,677 to 102,312
_0092
Psyr_0093: Psyr_0093 - Flavoprotein monooxygenase, at 102,358 to 103,761
_0093
Position (kb) 99
100
101
102 Strain fitness (log2 ratio) -2
-1
0
1
2 at 99.122 kb on + strand, within Psyr_0090 at 99.122 kb on + strand, within Psyr_0090 at 99.122 kb on + strand, within Psyr_0090 at 99.122 kb on + strand, within Psyr_0090 at 99.122 kb on + strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.270 kb on + strand, within Psyr_0090 at 99.270 kb on + strand, within Psyr_0090 at 99.509 kb on + strand, within Psyr_0090 at 99.509 kb on + strand, within Psyr_0090 at 99.509 kb on + strand, within Psyr_0090 at 99.510 kb on - strand, within Psyr_0090 at 99.510 kb on - strand, within Psyr_0090 at 99.510 kb on - strand, within Psyr_0090 at 99.510 kb on - strand, within Psyr_0090 at 99.518 kb on + strand, within Psyr_0090 at 99.519 kb on - strand, within Psyr_0090 at 99.526 kb on + strand, within Psyr_0090 at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.840 kb on + strand at 99.840 kb on + strand at 99.840 kb on + strand at 99.840 kb on + strand at 99.841 kb on - strand at 99.841 kb on - strand at 99.841 kb on - strand at 99.841 kb on - strand at 99.967 kb on + strand at 99.967 kb on + strand at 99.968 kb on - strand at 99.968 kb on - strand at 99.968 kb on - strand at 99.968 kb on - strand at 99.968 kb on - strand at 99.968 kb on - strand at 99.968 kb on - strand at 99.968 kb on - strand at 99.968 kb on - strand at 99.968 kb on - strand at 99.968 kb on - strand at 99.968 kb on - strand at 99.998 kb on - strand at 99.998 kb on - strand at 99.998 kb on - strand at 100.093 kb on + strand, within Psyr_0091 at 100.094 kb on - strand, within Psyr_0091 at 100.142 kb on - strand, within Psyr_0091 at 100.147 kb on + strand, within Psyr_0091 at 100.148 kb on - strand, within Psyr_0091 at 100.148 kb on - strand, within Psyr_0091 at 100.148 kb on - strand, within Psyr_0091 at 100.486 kb on + strand, within Psyr_0091 at 100.486 kb on + strand, within Psyr_0091 at 100.486 kb on + strand, within Psyr_0091 at 100.487 kb on - strand, within Psyr_0091 at 100.487 kb on - strand, within Psyr_0091 at 100.487 kb on - strand, within Psyr_0091 at 100.504 kb on + strand, within Psyr_0091 at 100.504 kb on + strand, within Psyr_0091 at 100.504 kb on + strand, within Psyr_0091 at 100.504 kb on + strand, within Psyr_0091 at 100.504 kb on + strand, within Psyr_0091 at 100.504 kb on + strand, within Psyr_0091 at 100.504 kb on + strand, within Psyr_0091 at 100.504 kb on + strand, within Psyr_0091 at 100.504 kb on + strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.546 kb on + strand, within Psyr_0091 at 101.011 kb on + strand, within Psyr_0091 at 101.011 kb on + strand, within Psyr_0091 at 101.012 kb on - strand, within Psyr_0091 at 101.012 kb on - strand, within Psyr_0091 at 101.012 kb on - strand, within Psyr_0091 at 101.059 kb on + strand, within Psyr_0091 at 101.059 kb on + strand, within Psyr_0091 at 101.059 kb on + strand, within Psyr_0091 at 101.059 kb on + strand, within Psyr_0091 at 101.060 kb on - strand, within Psyr_0091 at 101.060 kb on - strand, within Psyr_0091 at 101.060 kb on - strand, within Psyr_0091 at 101.060 kb on - strand, within Psyr_0091 at 101.060 kb on - strand, within Psyr_0091 at 101.350 kb on + strand at 101.350 kb on + strand at 101.350 kb on + strand at 101.350 kb on + strand at 101.350 kb on + strand at 101.350 kb on + strand at 101.350 kb on + strand at 101.351 kb on - strand at 101.582 kb on - strand at 101.724 kb on + strand at 101.724 kb on + strand at 101.725 kb on - strand at 101.810 kb on + strand, within Psyr_0092 at 101.810 kb on + strand, within Psyr_0092 at 101.810 kb on + strand, within Psyr_0092 at 101.811 kb on - strand, within Psyr_0092 at 101.811 kb on - strand, within Psyr_0092 at 101.811 kb on - strand, within Psyr_0092 at 101.811 kb on - strand, within Psyr_0092 at 101.958 kb on + strand, within Psyr_0092 at 101.958 kb on + strand, within Psyr_0092 at 101.958 kb on + strand, within Psyr_0092 at 101.959 kb on - strand, within Psyr_0092 at 101.959 kb on - strand, within Psyr_0092 at 101.968 kb on - strand, within Psyr_0092 at 102.057 kb on - strand, within Psyr_0092 at 102.057 kb on - strand, within Psyr_0092 at 102.057 kb on - strand, within Psyr_0092 at 102.301 kb on + strand at 102.302 kb on - strand at 102.317 kb on + strand at 102.317 kb on + strand at 102.317 kb on + strand at 102.359 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acriflavine 1.25 ug/ml remove 99,122 + Psyr_0090 0.49 -0.0 99,122 + Psyr_0090 0.49 +1.1 99,122 + Psyr_0090 0.49 -0.5 99,122 + Psyr_0090 0.49 +0.5 99,122 + Psyr_0090 0.49 -0.7 99,123 - Psyr_0090 0.50 -0.5 99,123 - Psyr_0090 0.50 +1.1 99,123 - Psyr_0090 0.50 +1.0 99,123 - Psyr_0090 0.50 -0.2 99,270 + Psyr_0090 0.61 +0.0 99,270 + Psyr_0090 0.61 +1.1 99,509 + Psyr_0090 0.80 +0.6 99,509 + Psyr_0090 0.80 +0.3 99,509 + Psyr_0090 0.80 +0.6 99,510 - Psyr_0090 0.80 -0.9 99,510 - Psyr_0090 0.80 -0.1 99,510 - Psyr_0090 0.80 +0.0 99,510 - Psyr_0090 0.80 +0.1 99,518 + Psyr_0090 0.80 -1.5 99,519 - Psyr_0090 0.80 -0.5 99,526 + Psyr_0090 0.81 -0.1 99,770 + +0.6 99,770 + -2.0 99,770 + +0.8 99,770 + +1.3 99,770 + -0.3 99,770 + -0.2 99,770 + +2.3 99,770 + +0.5 99,770 + +0.3 99,770 + +0.6 99,770 + +0.2 99,770 + -1.2 99,770 + +1.5 99,770 + +0.6 99,770 + +0.4 99,770 + -0.1 99,770 + -0.7 99,770 + -1.0 99,771 - -0.5 99,771 - +0.6 99,771 - +1.3 99,771 - +0.4 99,771 - +0.4 99,771 - +0.2 99,771 - +1.4 99,771 - +0.7 99,771 - -0.1 99,771 - +0.8 99,771 - +0.3 99,771 - -1.0 99,771 - +1.0 99,771 - -1.4 99,771 - -0.3 99,840 + -0.8 99,840 + -1.3 99,840 + +0.1 99,840 + +2.0 99,841 - -0.0 99,841 - +2.2 99,841 - -0.2 99,841 - -0.8 99,967 + +0.1 99,967 + -0.2 99,968 - +1.1 99,968 - +1.1 99,968 - +0.2 99,968 - +0.9 99,968 - -0.6 99,968 - +0.8 99,968 - +0.7 99,968 - -0.4 99,968 - +0.5 99,968 - -0.3 99,968 - -0.3 99,968 - -0.8 99,998 - -0.6 99,998 - +1.2 99,998 - -0.2 100,093 + Psyr_0091 0.10 +0.5 100,094 - Psyr_0091 0.10 +0.1 100,142 - Psyr_0091 0.13 -1.0 100,147 + Psyr_0091 0.14 +0.2 100,148 - Psyr_0091 0.14 +1.2 100,148 - Psyr_0091 0.14 +1.5 100,148 - Psyr_0091 0.14 +0.6 100,486 + Psyr_0091 0.37 +0.0 100,486 + Psyr_0091 0.37 +0.1 100,486 + Psyr_0091 0.37 +0.6 100,487 - Psyr_0091 0.37 +0.0 100,487 - Psyr_0091 0.37 -2.3 100,487 - Psyr_0091 0.37 +0.5 100,504 + Psyr_0091 0.39 +0.6 100,504 + Psyr_0091 0.39 +0.2 100,504 + Psyr_0091 0.39 -0.0 100,504 + Psyr_0091 0.39 -0.8 100,504 + Psyr_0091 0.39 +1.0 100,504 + Psyr_0091 0.39 -2.6 100,504 + Psyr_0091 0.39 +0.2 100,504 + Psyr_0091 0.39 +0.7 100,504 + Psyr_0091 0.39 +0.1 100,505 - Psyr_0091 0.39 +0.6 100,505 - Psyr_0091 0.39 -0.3 100,505 - Psyr_0091 0.39 +0.8 100,505 - Psyr_0091 0.39 +0.7 100,505 - Psyr_0091 0.39 -0.3 100,505 - Psyr_0091 0.39 +0.5 100,505 - Psyr_0091 0.39 -0.2 100,546 + Psyr_0091 0.41 -0.5 101,011 + Psyr_0091 0.74 -0.6 101,011 + Psyr_0091 0.74 -1.7 101,012 - Psyr_0091 0.74 +0.3 101,012 - Psyr_0091 0.74 -1.8 101,012 - Psyr_0091 0.74 +1.0 101,059 + Psyr_0091 0.77 +0.5 101,059 + Psyr_0091 0.77 -0.1 101,059 + Psyr_0091 0.77 -0.5 101,059 + Psyr_0091 0.77 +1.5 101,060 - Psyr_0091 0.77 +0.2 101,060 - Psyr_0091 0.77 -0.5 101,060 - Psyr_0091 0.77 +0.3 101,060 - Psyr_0091 0.77 +0.9 101,060 - Psyr_0091 0.77 -0.0 101,350 + +0.8 101,350 + +0.4 101,350 + +0.3 101,350 + +1.4 101,350 + -1.6 101,350 + -0.3 101,350 + +0.6 101,351 - -1.0 101,582 - -1.7 101,724 + -1.1 101,724 + +0.5 101,725 - +0.6 101,810 + Psyr_0092 0.21 -0.5 101,810 + Psyr_0092 0.21 -0.0 101,810 + Psyr_0092 0.21 +0.5 101,811 - Psyr_0092 0.21 +0.1 101,811 - Psyr_0092 0.21 -0.0 101,811 - Psyr_0092 0.21 +0.5 101,811 - Psyr_0092 0.21 +0.1 101,958 + Psyr_0092 0.44 -0.5 101,958 + Psyr_0092 0.44 -0.4 101,958 + Psyr_0092 0.44 -0.2 101,959 - Psyr_0092 0.44 -0.6 101,959 - Psyr_0092 0.44 -2.3 101,968 - Psyr_0092 0.46 -0.4 102,057 - Psyr_0092 0.60 -0.7 102,057 - Psyr_0092 0.60 +0.3 102,057 - Psyr_0092 0.60 -0.3 102,301 + -0.7 102,302 - +0.6 102,317 + +1.3 102,317 + -0.4 102,317 + +0.2 102,359 + +0.3
Or see this region's nucleotide sequence