Strain Fitness in Pseudomonas fluorescens GW456-L13 around PfGW456L13_221

Experiment: D,L-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPfGW456L13_219 and PfGW456L13_220 are separated by 243 nucleotidesPfGW456L13_220 and PfGW456L13_221 are separated by 427 nucleotidesPfGW456L13_221 and PfGW456L13_222 are separated by 332 nucleotidesPfGW456L13_222 and PfGW456L13_223 are separated by 12 nucleotides PfGW456L13_219 - Protein involved in catabolism of external DNA, at 256,789 to 257,628 _219 PfGW456L13_220 - hypothetical protein, at 257,872 to 258,108 _220 PfGW456L13_221 - Proline/sodium symporter PutP (TC 2.A.21.2.1) @ Propionate/sodium symporter, at 258,536 to 260,020 _221 PfGW456L13_222 - hypothetical protein, at 260,353 to 260,469 _222 PfGW456L13_223 - Transcriptional repressor of PutA and PutP / Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12), at 260,482 to 264,432 _223 Position (kb) 258 259 260 261Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 257.621 kb on + strandat 257.894 kb on - strandat 257.894 kb on - strandat 257.940 kb on + strand, within PfGW456L13_220at 257.940 kb on + strand, within PfGW456L13_220at 257.940 kb on + strand, within PfGW456L13_220at 257.940 kb on + strand, within PfGW456L13_220at 257.941 kb on - strand, within PfGW456L13_220at 257.941 kb on - strand, within PfGW456L13_220at 257.941 kb on - strand, within PfGW456L13_220at 257.941 kb on - strand, within PfGW456L13_220at 257.941 kb on - strand, within PfGW456L13_220at 258.019 kb on - strand, within PfGW456L13_220at 258.019 kb on - strand, within PfGW456L13_220at 258.113 kb on + strandat 258.113 kb on + strandat 258.123 kb on + strandat 258.123 kb on + strandat 258.151 kb on - strandat 258.151 kb on - strandat 258.187 kb on - strandat 258.317 kb on + strandat 258.317 kb on + strandat 258.318 kb on - strandat 258.318 kb on - strandat 258.318 kb on - strandat 258.481 kb on + strandat 258.482 kb on - strandat 258.552 kb on + strandat 258.621 kb on + strandat 258.622 kb on - strandat 258.622 kb on - strandat 258.932 kb on - strand, within PfGW456L13_221at 258.948 kb on + strand, within PfGW456L13_221at 258.948 kb on + strand, within PfGW456L13_221at 258.948 kb on + strand, within PfGW456L13_221at 258.948 kb on + strand, within PfGW456L13_221at 258.949 kb on - strand, within PfGW456L13_221at 258.949 kb on - strand, within PfGW456L13_221at 258.949 kb on - strand, within PfGW456L13_221at 259.137 kb on + strand, within PfGW456L13_221at 259.138 kb on - strand, within PfGW456L13_221at 259.138 kb on - strand, within PfGW456L13_221at 259.228 kb on + strand, within PfGW456L13_221at 259.278 kb on + strand, within PfGW456L13_221at 259.278 kb on + strand, within PfGW456L13_221at 259.279 kb on - strand, within PfGW456L13_221at 259.279 kb on - strand, within PfGW456L13_221at 259.454 kb on + strand, within PfGW456L13_221at 259.454 kb on + strand, within PfGW456L13_221at 259.454 kb on + strand, within PfGW456L13_221at 259.454 kb on + strand, within PfGW456L13_221at 259.454 kb on + strand, within PfGW456L13_221at 259.455 kb on - strand, within PfGW456L13_221at 259.500 kb on + strand, within PfGW456L13_221at 259.500 kb on + strand, within PfGW456L13_221at 259.500 kb on + strand, within PfGW456L13_221at 259.500 kb on + strand, within PfGW456L13_221at 259.500 kb on + strand, within PfGW456L13_221at 259.501 kb on - strand, within PfGW456L13_221at 259.501 kb on - strand, within PfGW456L13_221at 259.602 kb on + strand, within PfGW456L13_221at 259.602 kb on + strand, within PfGW456L13_221at 259.602 kb on + strand, within PfGW456L13_221at 259.602 kb on + strand, within PfGW456L13_221at 259.602 kb on + strand, within PfGW456L13_221at 259.602 kb on + strand, within PfGW456L13_221at 259.602 kb on + strand, within PfGW456L13_221at 259.602 kb on + strand, within PfGW456L13_221at 259.602 kb on + strand, within PfGW456L13_221at 259.602 kb on + strand, within PfGW456L13_221at 259.602 kb on + strand, within PfGW456L13_221at 259.602 kb on + strand, within PfGW456L13_221at 259.602 kb on + strand, within PfGW456L13_221at 259.602 kb on + strand, within PfGW456L13_221at 259.602 kb on + strand, within PfGW456L13_221at 259.602 kb on + strand, within PfGW456L13_221at 259.602 kb on + strand, within PfGW456L13_221at 259.602 kb on + strand, within PfGW456L13_221at 259.602 kb on + strand, within PfGW456L13_221at 259.602 kb on + strand, within PfGW456L13_221at 259.603 kb on - strand, within PfGW456L13_221at 259.603 kb on - strand, within PfGW456L13_221at 259.603 kb on - strand, within PfGW456L13_221at 259.603 kb on - strand, within PfGW456L13_221at 259.603 kb on - strand, within PfGW456L13_221at 259.603 kb on - strand, within PfGW456L13_221at 259.603 kb on - strand, within PfGW456L13_221at 259.603 kb on - strand, within PfGW456L13_221at 259.603 kb on - strand, within PfGW456L13_221at 259.603 kb on - strand, within PfGW456L13_221at 259.603 kb on - strand, within PfGW456L13_221at 259.603 kb on - strand, within PfGW456L13_221at 259.603 kb on - strand, within PfGW456L13_221at 259.603 kb on - strand, within PfGW456L13_221at 259.603 kb on - strand, within PfGW456L13_221at 259.603 kb on - strand, within PfGW456L13_221at 259.684 kb on - strand, within PfGW456L13_221at 259.762 kb on - strand, within PfGW456L13_221at 259.815 kb on + strand, within PfGW456L13_221at 259.815 kb on + strand, within PfGW456L13_221at 259.815 kb on + strand, within PfGW456L13_221at 259.815 kb on + strand, within PfGW456L13_221at 259.815 kb on + strand, within PfGW456L13_221at 259.815 kb on + strand, within PfGW456L13_221at 259.815 kb on + strand, within PfGW456L13_221at 259.815 kb on + strand, within PfGW456L13_221at 259.815 kb on + strand, within PfGW456L13_221at 259.815 kb on + strand, within PfGW456L13_221at 259.815 kb on + strand, within PfGW456L13_221at 259.816 kb on - strand, within PfGW456L13_221at 259.816 kb on - strand, within PfGW456L13_221at 259.816 kb on - strand, within PfGW456L13_221at 259.816 kb on - strand, within PfGW456L13_221at 259.816 kb on - strand, within PfGW456L13_221at 259.816 kb on - strand, within PfGW456L13_221at 259.816 kb on - strand, within PfGW456L13_221at 259.816 kb on - strand, within PfGW456L13_221at 259.816 kb on - strand, within PfGW456L13_221at 259.917 kb on + strandat 259.980 kb on + strandat 259.980 kb on + strandat 259.980 kb on + strandat 259.981 kb on - strandat 259.981 kb on - strandat 259.981 kb on - strandat 260.012 kb on + strandat 260.013 kb on - strandat 260.013 kb on - strandat 260.021 kb on + strandat 260.021 kb on + strandat 260.021 kb on + strandat 260.022 kb on - strandat 260.022 kb on - strandat 260.022 kb on - strandat 260.026 kb on + strandat 260.026 kb on + strandat 260.045 kb on + strandat 260.045 kb on + strandat 260.045 kb on + strandat 260.045 kb on + strandat 260.045 kb on + strandat 260.046 kb on - strandat 260.046 kb on - strandat 260.046 kb on - strandat 260.046 kb on - strandat 260.049 kb on + strandat 260.049 kb on + strandat 260.050 kb on - strandat 260.050 kb on - strandat 260.081 kb on + strandat 260.081 kb on + strandat 260.082 kb on - strandat 260.082 kb on - strandat 260.082 kb on - strandat 260.116 kb on - strandat 260.116 kb on - strandat 260.116 kb on - strandat 260.116 kb on - strandat 260.116 kb on - strandat 260.194 kb on - strandat 260.194 kb on - strandat 260.194 kb on - strandat 260.209 kb on + strandat 260.210 kb on - strandat 260.234 kb on + strandat 260.313 kb on + strandat 260.354 kb on + strandat 260.355 kb on - strandat 260.376 kb on + strand, within PfGW456L13_222at 260.599 kb on + strandat 260.599 kb on + strandat 260.600 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D,L-Lactate (C)
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257,621 + +1.1
257,894 - -2.2
257,894 - +0.8
257,940 + PfGW456L13_220 0.29 -0.4
257,940 + PfGW456L13_220 0.29 +2.6
257,940 + PfGW456L13_220 0.29 -0.2
257,940 + PfGW456L13_220 0.29 +1.2
257,941 - PfGW456L13_220 0.29 +1.6
257,941 - PfGW456L13_220 0.29 -2.0
257,941 - PfGW456L13_220 0.29 -0.5
257,941 - PfGW456L13_220 0.29 +1.1
257,941 - PfGW456L13_220 0.29 +0.0
258,019 - PfGW456L13_220 0.62 -2.5
258,019 - PfGW456L13_220 0.62 +1.1
258,113 + +0.0
258,113 + -0.5
258,123 + +2.5
258,123 + +1.9
258,151 - -0.0
258,151 - -0.4
258,187 - +0.1
258,317 + +2.1
258,317 + -1.6
258,318 - -0.4
258,318 - -0.9
258,318 - -0.8
258,481 + +0.0
258,482 - -2.6
258,552 + -1.4
258,621 + -2.4
258,622 - +0.0
258,622 - +1.5
258,932 - PfGW456L13_221 0.27 +1.8
258,948 + PfGW456L13_221 0.28 +1.0
258,948 + PfGW456L13_221 0.28 +0.4
258,948 + PfGW456L13_221 0.28 -0.7
258,948 + PfGW456L13_221 0.28 -2.2
258,949 - PfGW456L13_221 0.28 +0.8
258,949 - PfGW456L13_221 0.28 -3.2
258,949 - PfGW456L13_221 0.28 -1.1
259,137 + PfGW456L13_221 0.40 -2.0
259,138 - PfGW456L13_221 0.41 +1.4
259,138 - PfGW456L13_221 0.41 +4.2
259,228 + PfGW456L13_221 0.47 -1.3
259,278 + PfGW456L13_221 0.50 -0.0
259,278 + PfGW456L13_221 0.50 -1.9
259,279 - PfGW456L13_221 0.50 +0.9
259,279 - PfGW456L13_221 0.50 +0.4
259,454 + PfGW456L13_221 0.62 +0.2
259,454 + PfGW456L13_221 0.62 -2.8
259,454 + PfGW456L13_221 0.62 -0.4
259,454 + PfGW456L13_221 0.62 +0.0
259,454 + PfGW456L13_221 0.62 -2.3
259,455 - PfGW456L13_221 0.62 +0.3
259,500 + PfGW456L13_221 0.65 -1.0
259,500 + PfGW456L13_221 0.65 -0.2
259,500 + PfGW456L13_221 0.65 +1.7
259,500 + PfGW456L13_221 0.65 +0.2
259,500 + PfGW456L13_221 0.65 -3.1
259,501 - PfGW456L13_221 0.65 +0.8
259,501 - PfGW456L13_221 0.65 -0.9
259,602 + PfGW456L13_221 0.72 +1.6
259,602 + PfGW456L13_221 0.72 -2.9
259,602 + PfGW456L13_221 0.72 -0.7
259,602 + PfGW456L13_221 0.72 +0.9
259,602 + PfGW456L13_221 0.72 +1.4
259,602 + PfGW456L13_221 0.72 +2.9
259,602 + PfGW456L13_221 0.72 +0.8
259,602 + PfGW456L13_221 0.72 -0.5
259,602 + PfGW456L13_221 0.72 -0.3
259,602 + PfGW456L13_221 0.72 +1.3
259,602 + PfGW456L13_221 0.72 +0.8
259,602 + PfGW456L13_221 0.72 -1.3
259,602 + PfGW456L13_221 0.72 -2.0
259,602 + PfGW456L13_221 0.72 -1.0
259,602 + PfGW456L13_221 0.72 +1.1
259,602 + PfGW456L13_221 0.72 +0.6
259,602 + PfGW456L13_221 0.72 -1.0
259,602 + PfGW456L13_221 0.72 -1.1
259,602 + PfGW456L13_221 0.72 +0.7
259,602 + PfGW456L13_221 0.72 -3.7
259,603 - PfGW456L13_221 0.72 -0.6
259,603 - PfGW456L13_221 0.72 +1.0
259,603 - PfGW456L13_221 0.72 -0.9
259,603 - PfGW456L13_221 0.72 +0.7
259,603 - PfGW456L13_221 0.72 +1.1
259,603 - PfGW456L13_221 0.72 +0.6
259,603 - PfGW456L13_221 0.72 +1.7
259,603 - PfGW456L13_221 0.72 -1.4
259,603 - PfGW456L13_221 0.72 +0.2
259,603 - PfGW456L13_221 0.72 -2.0
259,603 - PfGW456L13_221 0.72 -2.6
259,603 - PfGW456L13_221 0.72 -0.1
259,603 - PfGW456L13_221 0.72 -1.2
259,603 - PfGW456L13_221 0.72 -0.2
259,603 - PfGW456L13_221 0.72 -0.3
259,603 - PfGW456L13_221 0.72 -3.0
259,684 - PfGW456L13_221 0.77 -2.9
259,762 - PfGW456L13_221 0.83 +1.0
259,815 + PfGW456L13_221 0.86 +0.7
259,815 + PfGW456L13_221 0.86 +0.1
259,815 + PfGW456L13_221 0.86 +0.9
259,815 + PfGW456L13_221 0.86 -1.9
259,815 + PfGW456L13_221 0.86 -1.9
259,815 + PfGW456L13_221 0.86 +1.0
259,815 + PfGW456L13_221 0.86 +0.2
259,815 + PfGW456L13_221 0.86 +0.2
259,815 + PfGW456L13_221 0.86 +1.2
259,815 + PfGW456L13_221 0.86 +2.2
259,815 + PfGW456L13_221 0.86 -2.7
259,816 - PfGW456L13_221 0.86 -0.8
259,816 - PfGW456L13_221 0.86 +0.4
259,816 - PfGW456L13_221 0.86 +1.4
259,816 - PfGW456L13_221 0.86 -0.4
259,816 - PfGW456L13_221 0.86 +0.2
259,816 - PfGW456L13_221 0.86 +1.7
259,816 - PfGW456L13_221 0.86 +0.1
259,816 - PfGW456L13_221 0.86 +1.6
259,816 - PfGW456L13_221 0.86 +1.7
259,917 + -3.0
259,980 + -1.1
259,980 + -0.5
259,980 + -3.4
259,981 - -2.7
259,981 - -0.1
259,981 - +0.4
260,012 + +0.6
260,013 - -1.6
260,013 - -3.5
260,021 + +0.3
260,021 + -0.3
260,021 + +0.2
260,022 - +0.7
260,022 - -0.9
260,022 - -2.5
260,026 + +1.2
260,026 + -1.2
260,045 + +1.6
260,045 + +0.1
260,045 + -3.0
260,045 + +0.3
260,045 + -1.3
260,046 - -2.7
260,046 - +0.6
260,046 - -1.7
260,046 - +1.4
260,049 + +0.8
260,049 + -0.8
260,050 - -0.7
260,050 - +0.4
260,081 + +0.6
260,081 + +0.3
260,082 - -1.1
260,082 - +0.8
260,082 - +1.7
260,116 - -0.3
260,116 - +1.2
260,116 - -1.2
260,116 - -0.9
260,116 - +1.5
260,194 - -0.1
260,194 - -3.4
260,194 - +2.2
260,209 + +1.7
260,210 - -0.2
260,234 + -0.7
260,313 + -0.9
260,354 + -2.1
260,355 - -0.9
260,376 + PfGW456L13_222 0.20 -0.0
260,599 + -2.4
260,599 + -0.7
260,600 - -1.1

Or see this region's nucleotide sequence