Experiment: PYE with Dimethyl Sulfoxide 0.4 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_03085 and CCNA_03086 are separated by 63 nucleotides CCNA_03086 and CCNA_03087 are separated by 330 nucleotides CCNA_03087 and CCNA_03088 overlap by 8 nucleotides
CCNA_03085: CCNA_03085 - nitropropane dioxygenase/trans-enoyl-CoA reductase family, at 3,234,537 to 3,235,514
_03085
CCNA_03086: CCNA_03086 - hypothetical protein, at 3,235,578 to 3,236,024
_03086
CCNA_03087: CCNA_03087 - H-NOX family heme binding protein, at 3,236,355 to 3,236,915
_03087
CCNA_03088: CCNA_03088 - two-component sensor histidine kinase, at 3,236,908 to 3,238,782
_03088
Position (kb)
3235
3236
3237 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3234.614 kb on - strand at 3234.636 kb on + strand, within CCNA_03085 at 3234.644 kb on - strand, within CCNA_03085 at 3234.650 kb on - strand, within CCNA_03085 at 3234.673 kb on + strand, within CCNA_03085 at 3234.687 kb on - strand, within CCNA_03085 at 3234.760 kb on + strand, within CCNA_03085 at 3234.768 kb on - strand, within CCNA_03085 at 3234.770 kb on + strand, within CCNA_03085 at 3234.770 kb on + strand, within CCNA_03085 at 3234.770 kb on - strand, within CCNA_03085 at 3234.805 kb on + strand, within CCNA_03085 at 3234.805 kb on + strand, within CCNA_03085 at 3234.807 kb on + strand, within CCNA_03085 at 3234.813 kb on - strand, within CCNA_03085 at 3234.815 kb on + strand, within CCNA_03085 at 3234.815 kb on - strand, within CCNA_03085 at 3234.866 kb on - strand, within CCNA_03085 at 3234.941 kb on + strand, within CCNA_03085 at 3235.008 kb on - strand, within CCNA_03085 at 3235.031 kb on + strand, within CCNA_03085 at 3235.039 kb on - strand, within CCNA_03085 at 3235.081 kb on - strand, within CCNA_03085 at 3235.098 kb on + strand, within CCNA_03085 at 3235.165 kb on - strand, within CCNA_03085 at 3235.165 kb on - strand, within CCNA_03085 at 3235.165 kb on - strand, within CCNA_03085 at 3235.179 kb on - strand, within CCNA_03085 at 3235.179 kb on - strand, within CCNA_03085 at 3235.189 kb on - strand, within CCNA_03085 at 3235.193 kb on + strand, within CCNA_03085 at 3235.198 kb on + strand, within CCNA_03085 at 3235.199 kb on - strand, within CCNA_03085 at 3235.199 kb on - strand, within CCNA_03085 at 3235.199 kb on - strand, within CCNA_03085 at 3235.199 kb on - strand, within CCNA_03085 at 3235.261 kb on + strand, within CCNA_03085 at 3235.269 kb on - strand, within CCNA_03085 at 3235.269 kb on - strand, within CCNA_03085 at 3235.269 kb on - strand, within CCNA_03085 at 3235.298 kb on + strand, within CCNA_03085 at 3235.298 kb on + strand, within CCNA_03085 at 3235.325 kb on + strand, within CCNA_03085 at 3235.363 kb on + strand, within CCNA_03085 at 3235.369 kb on + strand, within CCNA_03085 at 3235.371 kb on - strand, within CCNA_03085 at 3235.371 kb on - strand, within CCNA_03085 at 3235.371 kb on - strand, within CCNA_03085 at 3235.371 kb on - strand, within CCNA_03085 at 3235.374 kb on + strand, within CCNA_03085 at 3235.374 kb on + strand, within CCNA_03085 at 3235.382 kb on - strand, within CCNA_03085 at 3235.400 kb on - strand, within CCNA_03085 at 3235.418 kb on - strand at 3235.435 kb on - strand at 3235.485 kb on - strand at 3235.487 kb on + strand at 3235.495 kb on - strand at 3235.527 kb on - strand at 3235.552 kb on - strand at 3235.596 kb on - strand at 3235.596 kb on - strand at 3235.648 kb on + strand, within CCNA_03086 at 3235.648 kb on + strand, within CCNA_03086 at 3235.657 kb on - strand, within CCNA_03086 at 3235.669 kb on + strand, within CCNA_03086 at 3235.669 kb on + strand, within CCNA_03086 at 3235.677 kb on - strand, within CCNA_03086 at 3235.677 kb on - strand, within CCNA_03086 at 3235.677 kb on - strand, within CCNA_03086 at 3235.677 kb on - strand, within CCNA_03086 at 3235.737 kb on + strand, within CCNA_03086 at 3235.770 kb on - strand, within CCNA_03086 at 3235.782 kb on + strand, within CCNA_03086 at 3235.790 kb on - strand, within CCNA_03086 at 3235.823 kb on + strand, within CCNA_03086 at 3235.831 kb on - strand, within CCNA_03086 at 3235.876 kb on + strand, within CCNA_03086 at 3235.884 kb on - strand, within CCNA_03086 at 3235.884 kb on - strand, within CCNA_03086 at 3235.884 kb on - strand, within CCNA_03086 at 3235.939 kb on + strand, within CCNA_03086 at 3236.015 kb on - strand at 3236.031 kb on - strand at 3236.043 kb on - strand at 3236.055 kb on - strand at 3236.090 kb on + strand at 3236.092 kb on + strand at 3236.139 kb on + strand at 3236.163 kb on + strand at 3236.171 kb on - strand at 3236.320 kb on + strand at 3236.321 kb on - strand at 3236.329 kb on - strand at 3236.350 kb on + strand at 3236.368 kb on + strand at 3236.428 kb on + strand, within CCNA_03087 at 3236.491 kb on + strand, within CCNA_03087 at 3236.491 kb on + strand, within CCNA_03087 at 3236.491 kb on + strand, within CCNA_03087 at 3236.554 kb on + strand, within CCNA_03087 at 3236.554 kb on + strand, within CCNA_03087 at 3236.557 kb on - strand, within CCNA_03087 at 3236.571 kb on + strand, within CCNA_03087 at 3236.588 kb on + strand, within CCNA_03087 at 3236.588 kb on + strand, within CCNA_03087 at 3236.588 kb on + strand, within CCNA_03087 at 3236.596 kb on - strand, within CCNA_03087 at 3236.603 kb on + strand, within CCNA_03087 at 3236.610 kb on + strand, within CCNA_03087 at 3236.613 kb on + strand, within CCNA_03087 at 3236.618 kb on - strand, within CCNA_03087 at 3236.629 kb on + strand, within CCNA_03087 at 3236.669 kb on + strand, within CCNA_03087 at 3236.675 kb on + strand, within CCNA_03087 at 3236.741 kb on + strand, within CCNA_03087 at 3236.742 kb on + strand, within CCNA_03087 at 3236.749 kb on - strand, within CCNA_03087 at 3236.774 kb on + strand, within CCNA_03087 at 3236.782 kb on + strand, within CCNA_03087 at 3236.788 kb on - strand, within CCNA_03087 at 3236.788 kb on - strand, within CCNA_03087 at 3236.816 kb on + strand, within CCNA_03087 at 3236.817 kb on + strand, within CCNA_03087 at 3236.817 kb on + strand, within CCNA_03087 at 3236.824 kb on + strand, within CCNA_03087 at 3236.824 kb on + strand, within CCNA_03087 at 3236.832 kb on - strand, within CCNA_03087 at 3236.868 kb on + strand at 3236.951 kb on - strand at 3236.953 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Dimethyl Sulfoxide 0.4 vol% remove 3,234,614 - +0.4 3,234,636 + CCNA_03085 0.10 -0.1 3,234,644 - CCNA_03085 0.11 -0.1 3,234,650 - CCNA_03085 0.12 +0.6 3,234,673 + CCNA_03085 0.14 +0.2 3,234,687 - CCNA_03085 0.15 -0.8 3,234,760 + CCNA_03085 0.23 -0.7 3,234,768 - CCNA_03085 0.24 +0.3 3,234,770 + CCNA_03085 0.24 +1.3 3,234,770 + CCNA_03085 0.24 -2.2 3,234,770 - CCNA_03085 0.24 +0.2 3,234,805 + CCNA_03085 0.27 +0.3 3,234,805 + CCNA_03085 0.27 -0.4 3,234,807 + CCNA_03085 0.28 -2.1 3,234,813 - CCNA_03085 0.28 -0.6 3,234,815 + CCNA_03085 0.28 -0.6 3,234,815 - CCNA_03085 0.28 -0.3 3,234,866 - CCNA_03085 0.34 -0.6 3,234,941 + CCNA_03085 0.41 +0.5 3,235,008 - CCNA_03085 0.48 +0.8 3,235,031 + CCNA_03085 0.51 -0.9 3,235,039 - CCNA_03085 0.51 -0.3 3,235,081 - CCNA_03085 0.56 -0.1 3,235,098 + CCNA_03085 0.57 +0.2 3,235,165 - CCNA_03085 0.64 +0.2 3,235,165 - CCNA_03085 0.64 -0.2 3,235,165 - CCNA_03085 0.64 +0.6 3,235,179 - CCNA_03085 0.66 -1.2 3,235,179 - CCNA_03085 0.66 +0.5 3,235,189 - CCNA_03085 0.67 -1.4 3,235,193 + CCNA_03085 0.67 -0.5 3,235,198 + CCNA_03085 0.68 -2.1 3,235,199 - CCNA_03085 0.68 +0.9 3,235,199 - CCNA_03085 0.68 +0.2 3,235,199 - CCNA_03085 0.68 +0.0 3,235,199 - CCNA_03085 0.68 +0.6 3,235,261 + CCNA_03085 0.74 +0.1 3,235,269 - CCNA_03085 0.75 -0.5 3,235,269 - CCNA_03085 0.75 -0.6 3,235,269 - CCNA_03085 0.75 -0.7 3,235,298 + CCNA_03085 0.78 -0.6 3,235,298 + CCNA_03085 0.78 -0.3 3,235,325 + CCNA_03085 0.81 -1.2 3,235,363 + CCNA_03085 0.84 -0.0 3,235,369 + CCNA_03085 0.85 -1.0 3,235,371 - CCNA_03085 0.85 -0.7 3,235,371 - CCNA_03085 0.85 -0.6 3,235,371 - CCNA_03085 0.85 +0.3 3,235,371 - CCNA_03085 0.85 -0.5 3,235,374 + CCNA_03085 0.86 +0.6 3,235,374 + CCNA_03085 0.86 -0.7 3,235,382 - CCNA_03085 0.86 -0.5 3,235,400 - CCNA_03085 0.88 -0.2 3,235,418 - -0.2 3,235,435 - +1.4 3,235,485 - +0.0 3,235,487 + -0.4 3,235,495 - +0.5 3,235,527 - +1.0 3,235,552 - +0.4 3,235,596 - +0.5 3,235,596 - -0.7 3,235,648 + CCNA_03086 0.16 +0.7 3,235,648 + CCNA_03086 0.16 -0.4 3,235,657 - CCNA_03086 0.18 +0.7 3,235,669 + CCNA_03086 0.20 -0.1 3,235,669 + CCNA_03086 0.20 +0.1 3,235,677 - CCNA_03086 0.22 +0.6 3,235,677 - CCNA_03086 0.22 -0.4 3,235,677 - CCNA_03086 0.22 +0.7 3,235,677 - CCNA_03086 0.22 -0.7 3,235,737 + CCNA_03086 0.36 +1.3 3,235,770 - CCNA_03086 0.43 -0.1 3,235,782 + CCNA_03086 0.46 +0.6 3,235,790 - CCNA_03086 0.47 +1.0 3,235,823 + CCNA_03086 0.55 +1.3 3,235,831 - CCNA_03086 0.57 +0.5 3,235,876 + CCNA_03086 0.67 +0.1 3,235,884 - CCNA_03086 0.68 +1.0 3,235,884 - CCNA_03086 0.68 -0.1 3,235,884 - CCNA_03086 0.68 +0.1 3,235,939 + CCNA_03086 0.81 -0.2 3,236,015 - +0.8 3,236,031 - -0.2 3,236,043 - +0.0 3,236,055 - -0.2 3,236,090 + -0.6 3,236,092 + +0.2 3,236,139 + -0.4 3,236,163 + +0.4 3,236,171 - -1.0 3,236,320 + +0.2 3,236,321 - -0.1 3,236,329 - -1.8 3,236,350 + +0.1 3,236,368 + +0.4 3,236,428 + CCNA_03087 0.13 +0.8 3,236,491 + CCNA_03087 0.24 +0.6 3,236,491 + CCNA_03087 0.24 -0.2 3,236,491 + CCNA_03087 0.24 -0.4 3,236,554 + CCNA_03087 0.35 -0.1 3,236,554 + CCNA_03087 0.35 -0.4 3,236,557 - CCNA_03087 0.36 +0.8 3,236,571 + CCNA_03087 0.39 -0.7 3,236,588 + CCNA_03087 0.42 +0.2 3,236,588 + CCNA_03087 0.42 -0.6 3,236,588 + CCNA_03087 0.42 -0.2 3,236,596 - CCNA_03087 0.43 -0.0 3,236,603 + CCNA_03087 0.44 -0.8 3,236,610 + CCNA_03087 0.45 +0.2 3,236,613 + CCNA_03087 0.46 -0.1 3,236,618 - CCNA_03087 0.47 +1.6 3,236,629 + CCNA_03087 0.49 -1.0 3,236,669 + CCNA_03087 0.56 +0.1 3,236,675 + CCNA_03087 0.57 -0.9 3,236,741 + CCNA_03087 0.69 -0.7 3,236,742 + CCNA_03087 0.69 +0.1 3,236,749 - CCNA_03087 0.70 +2.3 3,236,774 + CCNA_03087 0.75 +1.2 3,236,782 + CCNA_03087 0.76 +0.2 3,236,788 - CCNA_03087 0.77 +0.2 3,236,788 - CCNA_03087 0.77 -0.7 3,236,816 + CCNA_03087 0.82 +0.1 3,236,817 + CCNA_03087 0.82 -0.0 3,236,817 + CCNA_03087 0.82 +0.0 3,236,824 + CCNA_03087 0.84 +0.6 3,236,824 + CCNA_03087 0.84 -0.1 3,236,832 - CCNA_03087 0.85 -2.4 3,236,868 + +0.6 3,236,951 - -0.6 3,236,953 + -1.0
Or see this region's nucleotide sequence