Experiment: PYE with Dimethyl Sulfoxide 0.4 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01988 and CCNA_01989 overlap by 4 nucleotides CCNA_01989 and CCNA_01990 are separated by 2 nucleotides CCNA_01990 and CCNA_01991 are separated by 53 nucleotides CCNA_01991 and CCNA_01992 are separated by 2 nucleotides
CCNA_01988: CCNA_01988 - UDP-N-acetylglucosamine O-acyltransferase LpxA, at 2,134,332 to 2,135,123
_01988
CCNA_01989: CCNA_01989 - (3R)-hydroxymyristoyl-ACP dehydratase FabZ, at 2,135,120 to 2,135,599
_01989
CCNA_01990: CCNA_01990 - UDP-3-O-(3-hydroxymyristoyl) glucosamine N-acyltransferase LpxD, at 2,135,602 to 2,136,621
_01990
CCNA_01991: CCNA_01991 - OmpH-like outer membrane protein, at 2,136,675 to 2,137,313
_01991
CCNA_01992: CCNA_01992 - beta-barrel assembly machine (BAM) protein BamA, at 2,137,316 to 2,139,679
_01992
Position (kb)
2135
2136
2137 Strain fitness (log2 ratio)
-1
0
1 at 2134.608 kb on - strand, within CCNA_01988 at 2134.648 kb on + strand, within CCNA_01988 at 2134.804 kb on - strand, within CCNA_01988 at 2134.819 kb on + strand, within CCNA_01988 at 2135.017 kb on + strand, within CCNA_01988 at 2135.134 kb on - strand at 2135.804 kb on - strand, within CCNA_01990 at 2135.885 kb on + strand, within CCNA_01990
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Dimethyl Sulfoxide 0.4 vol% remove 2,134,608 - CCNA_01988 0.35 -0.7 2,134,648 + CCNA_01988 0.40 +0.1 2,134,804 - CCNA_01988 0.60 +0.3 2,134,819 + CCNA_01988 0.61 -0.4 2,135,017 + CCNA_01988 0.86 -0.9 2,135,134 - +0.3 2,135,804 - CCNA_01990 0.20 +0.3 2,135,885 + CCNA_01990 0.28 -0.7
Or see this region's nucleotide sequence