Experiment: PYE with Dimethyl Sulfoxide 0.4 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01588 and CCNA_01589 are separated by 4 nucleotides CCNA_01589 and CCNA_01590 are separated by 132 nucleotides
CCNA_01588: CCNA_01588 - hypothetical protein, at 1,706,752 to 1,708,107
_01588
CCNA_01589: CCNA_01589 - acyltransferase 3, at 1,708,112 to 1,709,248
_01589
CCNA_01590: CCNA_01590 - NAD-dependent DNA ligase, at 1,709,381 to 1,711,732
_01590
Position (kb)
1708
1709
1710 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1707.177 kb on + strand, within CCNA_01588 at 1707.185 kb on - strand, within CCNA_01588 at 1707.212 kb on - strand, within CCNA_01588 at 1707.239 kb on + strand, within CCNA_01588 at 1707.239 kb on - strand, within CCNA_01588 at 1707.270 kb on + strand, within CCNA_01588 at 1707.278 kb on + strand, within CCNA_01588 at 1707.283 kb on + strand, within CCNA_01588 at 1707.291 kb on - strand, within CCNA_01588 at 1707.335 kb on - strand, within CCNA_01588 at 1707.402 kb on - strand, within CCNA_01588 at 1707.402 kb on - strand, within CCNA_01588 at 1707.421 kb on + strand, within CCNA_01588 at 1707.426 kb on + strand, within CCNA_01588 at 1707.429 kb on - strand, within CCNA_01588 at 1707.433 kb on + strand, within CCNA_01588 at 1707.433 kb on + strand, within CCNA_01588 at 1707.433 kb on + strand, within CCNA_01588 at 1707.441 kb on - strand, within CCNA_01588 at 1707.441 kb on - strand, within CCNA_01588 at 1707.476 kb on + strand, within CCNA_01588 at 1707.496 kb on + strand, within CCNA_01588 at 1707.497 kb on + strand, within CCNA_01588 at 1707.545 kb on + strand, within CCNA_01588 at 1707.545 kb on - strand, within CCNA_01588 at 1707.550 kb on - strand, within CCNA_01588 at 1707.555 kb on - strand, within CCNA_01588 at 1707.563 kb on + strand, within CCNA_01588 at 1707.563 kb on - strand, within CCNA_01588 at 1707.569 kb on + strand, within CCNA_01588 at 1707.569 kb on + strand, within CCNA_01588 at 1707.571 kb on - strand, within CCNA_01588 at 1707.571 kb on - strand, within CCNA_01588 at 1707.577 kb on - strand, within CCNA_01588 at 1707.577 kb on - strand, within CCNA_01588 at 1707.588 kb on - strand, within CCNA_01588 at 1707.588 kb on - strand, within CCNA_01588 at 1707.592 kb on + strand, within CCNA_01588 at 1707.627 kb on + strand, within CCNA_01588 at 1707.627 kb on + strand, within CCNA_01588 at 1707.660 kb on - strand, within CCNA_01588 at 1707.663 kb on + strand, within CCNA_01588 at 1707.663 kb on + strand, within CCNA_01588 at 1707.663 kb on + strand, within CCNA_01588 at 1707.671 kb on - strand, within CCNA_01588 at 1707.671 kb on - strand, within CCNA_01588 at 1707.700 kb on + strand, within CCNA_01588 at 1707.708 kb on - strand, within CCNA_01588 at 1707.841 kb on - strand, within CCNA_01588 at 1707.865 kb on - strand, within CCNA_01588 at 1708.008 kb on - strand at 1708.032 kb on + strand at 1708.032 kb on + strand at 1708.032 kb on + strand at 1708.032 kb on + strand at 1708.045 kb on + strand at 1708.057 kb on + strand at 1708.088 kb on + strand at 1708.109 kb on + strand at 1708.172 kb on - strand at 1708.189 kb on - strand at 1708.224 kb on + strand at 1708.288 kb on + strand, within CCNA_01589 at 1708.296 kb on - strand, within CCNA_01589 at 1708.311 kb on + strand, within CCNA_01589 at 1708.336 kb on + strand, within CCNA_01589 at 1708.352 kb on - strand, within CCNA_01589 at 1708.357 kb on - strand, within CCNA_01589 at 1708.378 kb on + strand, within CCNA_01589 at 1708.402 kb on + strand, within CCNA_01589 at 1708.433 kb on + strand, within CCNA_01589 at 1708.455 kb on - strand, within CCNA_01589 at 1708.455 kb on - strand, within CCNA_01589 at 1708.502 kb on + strand, within CCNA_01589 at 1708.514 kb on + strand, within CCNA_01589 at 1708.514 kb on + strand, within CCNA_01589 at 1708.514 kb on + strand, within CCNA_01589 at 1708.536 kb on - strand, within CCNA_01589 at 1708.580 kb on - strand, within CCNA_01589 at 1708.600 kb on + strand, within CCNA_01589 at 1708.600 kb on + strand, within CCNA_01589 at 1708.600 kb on + strand, within CCNA_01589 at 1708.600 kb on + strand, within CCNA_01589 at 1708.600 kb on + strand, within CCNA_01589 at 1708.600 kb on + strand, within CCNA_01589 at 1708.603 kb on - strand, within CCNA_01589 at 1708.607 kb on + strand, within CCNA_01589 at 1708.608 kb on - strand, within CCNA_01589 at 1708.608 kb on - strand, within CCNA_01589 at 1708.608 kb on - strand, within CCNA_01589 at 1708.618 kb on + strand, within CCNA_01589 at 1708.618 kb on + strand, within CCNA_01589 at 1708.662 kb on + strand, within CCNA_01589 at 1708.715 kb on + strand, within CCNA_01589 at 1708.737 kb on + strand, within CCNA_01589 at 1708.737 kb on + strand, within CCNA_01589 at 1708.745 kb on - strand, within CCNA_01589 at 1708.747 kb on + strand, within CCNA_01589 at 1708.754 kb on + strand, within CCNA_01589 at 1708.774 kb on + strand, within CCNA_01589 at 1708.782 kb on - strand, within CCNA_01589 at 1708.784 kb on - strand, within CCNA_01589 at 1708.815 kb on + strand, within CCNA_01589 at 1708.815 kb on + strand, within CCNA_01589 at 1708.823 kb on - strand, within CCNA_01589 at 1708.823 kb on - strand, within CCNA_01589 at 1708.865 kb on - strand, within CCNA_01589 at 1708.894 kb on + strand, within CCNA_01589 at 1708.897 kb on - strand, within CCNA_01589 at 1708.927 kb on + strand, within CCNA_01589 at 1708.932 kb on + strand, within CCNA_01589 at 1708.963 kb on - strand, within CCNA_01589 at 1708.981 kb on - strand, within CCNA_01589 at 1708.990 kb on - strand, within CCNA_01589 at 1708.996 kb on - strand, within CCNA_01589 at 1708.997 kb on - strand, within CCNA_01589 at 1709.019 kb on + strand, within CCNA_01589 at 1709.027 kb on - strand, within CCNA_01589 at 1709.027 kb on - strand, within CCNA_01589 at 1709.027 kb on - strand, within CCNA_01589 at 1709.027 kb on - strand, within CCNA_01589 at 1709.100 kb on + strand, within CCNA_01589 at 1709.100 kb on + strand, within CCNA_01589 at 1709.150 kb on + strand at 1709.190 kb on + strand at 1709.190 kb on + strand at 1709.190 kb on + strand at 1709.200 kb on + strand at 1709.222 kb on + strand at 1709.238 kb on + strand at 1709.246 kb on - strand at 1709.325 kb on - strand at 1709.327 kb on + strand at 1709.332 kb on + strand at 1709.340 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Dimethyl Sulfoxide 0.4 vol% remove 1,707,177 + CCNA_01588 0.31 +0.4 1,707,185 - CCNA_01588 0.32 +0.9 1,707,212 - CCNA_01588 0.34 -1.2 1,707,239 + CCNA_01588 0.36 -2.5 1,707,239 - CCNA_01588 0.36 +0.7 1,707,270 + CCNA_01588 0.38 +0.5 1,707,278 + CCNA_01588 0.39 +0.9 1,707,283 + CCNA_01588 0.39 -0.0 1,707,291 - CCNA_01588 0.40 +0.2 1,707,335 - CCNA_01588 0.43 -1.2 1,707,402 - CCNA_01588 0.48 +0.1 1,707,402 - CCNA_01588 0.48 -1.1 1,707,421 + CCNA_01588 0.49 -0.0 1,707,426 + CCNA_01588 0.50 +0.1 1,707,429 - CCNA_01588 0.50 -1.0 1,707,433 + CCNA_01588 0.50 -0.5 1,707,433 + CCNA_01588 0.50 +0.0 1,707,433 + CCNA_01588 0.50 +0.2 1,707,441 - CCNA_01588 0.51 -0.3 1,707,441 - CCNA_01588 0.51 -1.2 1,707,476 + CCNA_01588 0.53 +0.4 1,707,496 + CCNA_01588 0.55 -0.0 1,707,497 + CCNA_01588 0.55 -0.3 1,707,545 + CCNA_01588 0.58 -0.1 1,707,545 - CCNA_01588 0.58 -0.6 1,707,550 - CCNA_01588 0.59 -0.1 1,707,555 - CCNA_01588 0.59 -1.4 1,707,563 + CCNA_01588 0.60 -0.3 1,707,563 - CCNA_01588 0.60 +0.1 1,707,569 + CCNA_01588 0.60 -0.9 1,707,569 + CCNA_01588 0.60 -0.5 1,707,571 - CCNA_01588 0.60 +1.2 1,707,571 - CCNA_01588 0.60 -0.3 1,707,577 - CCNA_01588 0.61 -0.7 1,707,577 - CCNA_01588 0.61 -0.3 1,707,588 - CCNA_01588 0.62 -0.7 1,707,588 - CCNA_01588 0.62 -1.2 1,707,592 + CCNA_01588 0.62 -0.2 1,707,627 + CCNA_01588 0.65 -1.4 1,707,627 + CCNA_01588 0.65 +0.8 1,707,660 - CCNA_01588 0.67 -1.2 1,707,663 + CCNA_01588 0.67 -0.2 1,707,663 + CCNA_01588 0.67 -0.3 1,707,663 + CCNA_01588 0.67 +0.0 1,707,671 - CCNA_01588 0.68 -1.1 1,707,671 - CCNA_01588 0.68 -1.5 1,707,700 + CCNA_01588 0.70 +0.8 1,707,708 - CCNA_01588 0.71 -1.2 1,707,841 - CCNA_01588 0.80 +0.9 1,707,865 - CCNA_01588 0.82 +0.2 1,708,008 - -0.6 1,708,032 + -0.0 1,708,032 + +0.2 1,708,032 + -0.5 1,708,032 + -0.5 1,708,045 + -0.4 1,708,057 + +0.1 1,708,088 + +0.1 1,708,109 + -0.4 1,708,172 - -1.5 1,708,189 - +0.0 1,708,224 + -1.0 1,708,288 + CCNA_01589 0.15 +1.7 1,708,296 - CCNA_01589 0.16 +0.4 1,708,311 + CCNA_01589 0.18 -1.7 1,708,336 + CCNA_01589 0.20 +0.6 1,708,352 - CCNA_01589 0.21 -0.3 1,708,357 - CCNA_01589 0.22 +1.0 1,708,378 + CCNA_01589 0.23 -2.1 1,708,402 + CCNA_01589 0.26 -0.3 1,708,433 + CCNA_01589 0.28 -0.4 1,708,455 - CCNA_01589 0.30 +0.4 1,708,455 - CCNA_01589 0.30 -0.2 1,708,502 + CCNA_01589 0.34 +0.7 1,708,514 + CCNA_01589 0.35 +0.6 1,708,514 + CCNA_01589 0.35 -1.4 1,708,514 + CCNA_01589 0.35 +0.1 1,708,536 - CCNA_01589 0.37 -0.2 1,708,580 - CCNA_01589 0.41 -0.7 1,708,600 + CCNA_01589 0.43 -0.3 1,708,600 + CCNA_01589 0.43 -0.5 1,708,600 + CCNA_01589 0.43 -1.3 1,708,600 + CCNA_01589 0.43 -1.3 1,708,600 + CCNA_01589 0.43 +0.4 1,708,600 + CCNA_01589 0.43 +0.1 1,708,603 - CCNA_01589 0.43 -0.1 1,708,607 + CCNA_01589 0.44 +0.8 1,708,608 - CCNA_01589 0.44 +0.4 1,708,608 - CCNA_01589 0.44 +0.8 1,708,608 - CCNA_01589 0.44 +0.7 1,708,618 + CCNA_01589 0.45 +1.7 1,708,618 + CCNA_01589 0.45 +1.1 1,708,662 + CCNA_01589 0.48 +0.4 1,708,715 + CCNA_01589 0.53 -0.4 1,708,737 + CCNA_01589 0.55 -2.4 1,708,737 + CCNA_01589 0.55 -1.9 1,708,745 - CCNA_01589 0.56 -0.2 1,708,747 + CCNA_01589 0.56 +0.4 1,708,754 + CCNA_01589 0.56 -0.5 1,708,774 + CCNA_01589 0.58 +2.1 1,708,782 - CCNA_01589 0.59 +2.0 1,708,784 - CCNA_01589 0.59 +0.5 1,708,815 + CCNA_01589 0.62 -0.6 1,708,815 + CCNA_01589 0.62 -1.0 1,708,823 - CCNA_01589 0.63 +1.7 1,708,823 - CCNA_01589 0.63 +0.4 1,708,865 - CCNA_01589 0.66 +1.3 1,708,894 + CCNA_01589 0.69 +0.6 1,708,897 - CCNA_01589 0.69 -0.6 1,708,927 + CCNA_01589 0.72 +0.4 1,708,932 + CCNA_01589 0.72 +0.2 1,708,963 - CCNA_01589 0.75 -0.7 1,708,981 - CCNA_01589 0.76 +0.8 1,708,990 - CCNA_01589 0.77 +1.1 1,708,996 - CCNA_01589 0.78 -0.7 1,708,997 - CCNA_01589 0.78 +0.2 1,709,019 + CCNA_01589 0.80 -0.2 1,709,027 - CCNA_01589 0.80 +0.8 1,709,027 - CCNA_01589 0.80 +1.3 1,709,027 - CCNA_01589 0.80 +0.1 1,709,027 - CCNA_01589 0.80 -0.1 1,709,100 + CCNA_01589 0.87 -1.0 1,709,100 + CCNA_01589 0.87 -0.2 1,709,150 + +0.0 1,709,190 + -2.1 1,709,190 + -0.6 1,709,190 + -1.1 1,709,200 + -0.6 1,709,222 + -1.3 1,709,238 + -0.3 1,709,246 - -1.6 1,709,325 - +0.3 1,709,327 + +0.5 1,709,332 + -0.5 1,709,340 + -0.4
Or see this region's nucleotide sequence