Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01452 and CCNA_R0038 are separated by 64 nucleotides CCNA_R0038 and CCNA_01453 are separated by 26 nucleotides CCNA_01453 and CCNA_R0039 are separated by 28 nucleotides CCNA_R0039 and CCNA_01457 are separated by 690 nucleotides CCNA_01457 and CCNA_01458 are separated by 2 nucleotides
CCNA_01452: CCNA_01452 - hypothetical protein, at 1,565,802 to 1,566,356
_01452
CCNA_R0038: CCNA_R0038 - tRNA-Pro, at 1,566,421 to 1,566,497
_R0038
CCNA_01453: CCNA_01453 - electron transport complex I subunit, NADH-ubiquinone oxidoreductase, at 1,566,524 to 1,566,829
_01453
CCNA_R0039: CCNA_R0039 - tRNA-Arg, at 1,566,858 to 1,566,934
_R0039
CCNA_01457: CCNA_01457 - hypothetical protein, at 1,567,625 to 1,567,876
_01457
CCNA_01458: CCNA_01458 - hypothetical protein, at 1,567,879 to 1,568,187
_01458
Position (kb)
1566
1567 Strain fitness (log2 ratio)
-1
0
1
2 at 1565.933 kb on - strand, within CCNA_01452 at 1565.964 kb on + strand, within CCNA_01452 at 1566.023 kb on + strand, within CCNA_01452 at 1566.115 kb on + strand, within CCNA_01452 at 1566.123 kb on - strand, within CCNA_01452 at 1566.243 kb on - strand, within CCNA_01452 at 1566.256 kb on - strand, within CCNA_01452 at 1566.272 kb on - strand, within CCNA_01452 at 1566.290 kb on + strand, within CCNA_01452 at 1566.300 kb on + strand, within CCNA_01452 at 1566.300 kb on + strand, within CCNA_01452 at 1566.306 kb on + strand at 1566.314 kb on + strand at 1566.384 kb on + strand at 1566.391 kb on + strand at 1566.394 kb on + strand at 1566.402 kb on - strand at 1566.519 kb on + strand at 1566.527 kb on - strand at 1566.574 kb on + strand, within CCNA_01453 at 1566.574 kb on + strand, within CCNA_01453 at 1566.581 kb on + strand, within CCNA_01453 at 1566.582 kb on - strand, within CCNA_01453 at 1566.582 kb on - strand, within CCNA_01453 at 1566.587 kb on - strand, within CCNA_01453 at 1566.642 kb on + strand, within CCNA_01453 at 1566.650 kb on + strand, within CCNA_01453 at 1566.684 kb on + strand, within CCNA_01453 at 1566.711 kb on + strand, within CCNA_01453 at 1566.719 kb on - strand, within CCNA_01453 at 1566.719 kb on - strand, within CCNA_01453 at 1566.781 kb on - strand, within CCNA_01453 at 1566.781 kb on - strand, within CCNA_01453 at 1566.814 kb on + strand at 1566.814 kb on + strand at 1566.814 kb on + strand at 1566.814 kb on + strand at 1566.814 kb on + strand at 1566.814 kb on + strand at 1566.814 kb on + strand at 1566.814 kb on + strand at 1566.814 kb on + strand at 1566.814 kb on + strand at 1566.822 kb on - strand at 1566.822 kb on - strand at 1567.018 kb on + strand at 1567.095 kb on + strand at 1567.097 kb on - strand at 1567.102 kb on - strand at 1567.102 kb on - strand at 1567.173 kb on + strand at 1567.225 kb on + strand at 1567.240 kb on + strand at 1567.243 kb on - strand at 1567.248 kb on - strand at 1567.269 kb on - strand at 1567.320 kb on + strand at 1567.379 kb on - strand at 1567.532 kb on + strand at 1567.684 kb on - strand, within CCNA_01457 at 1567.693 kb on - strand, within CCNA_01457 at 1567.706 kb on + strand, within CCNA_01457 at 1567.818 kb on + strand, within CCNA_01457 at 1567.857 kb on - strand at 1567.860 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 1,565,933 - CCNA_01452 0.24 -0.6 1,565,964 + CCNA_01452 0.29 +0.1 1,566,023 + CCNA_01452 0.40 +1.2 1,566,115 + CCNA_01452 0.56 -0.2 1,566,123 - CCNA_01452 0.58 -0.9 1,566,243 - CCNA_01452 0.79 +1.4 1,566,256 - CCNA_01452 0.82 -0.7 1,566,272 - CCNA_01452 0.85 +0.9 1,566,290 + CCNA_01452 0.88 +0.3 1,566,300 + CCNA_01452 0.90 +1.4 1,566,300 + CCNA_01452 0.90 -0.1 1,566,306 + +0.6 1,566,314 + -0.7 1,566,384 + -1.4 1,566,391 + +1.9 1,566,394 + +1.1 1,566,402 - +0.3 1,566,519 + -0.2 1,566,527 - +1.1 1,566,574 + CCNA_01453 0.16 +0.8 1,566,574 + CCNA_01453 0.16 -0.2 1,566,581 + CCNA_01453 0.19 -0.0 1,566,582 - CCNA_01453 0.19 -0.2 1,566,582 - CCNA_01453 0.19 -0.5 1,566,587 - CCNA_01453 0.21 +0.3 1,566,642 + CCNA_01453 0.39 -0.8 1,566,650 + CCNA_01453 0.41 -0.9 1,566,684 + CCNA_01453 0.52 -0.4 1,566,711 + CCNA_01453 0.61 +0.2 1,566,719 - CCNA_01453 0.64 -0.6 1,566,719 - CCNA_01453 0.64 -0.1 1,566,781 - CCNA_01453 0.84 +1.1 1,566,781 - CCNA_01453 0.84 +0.5 1,566,814 + -0.6 1,566,814 + +0.4 1,566,814 + -1.5 1,566,814 + -0.6 1,566,814 + +1.6 1,566,814 + -0.2 1,566,814 + +0.1 1,566,814 + +0.7 1,566,814 + +0.8 1,566,814 + -0.2 1,566,822 - -1.2 1,566,822 - +0.3 1,567,018 + -0.7 1,567,095 + -0.1 1,567,097 - +0.2 1,567,102 - -0.6 1,567,102 - -0.0 1,567,173 + +0.3 1,567,225 + +1.4 1,567,240 + -0.3 1,567,243 - -0.7 1,567,248 - -0.2 1,567,269 - +0.1 1,567,320 + +1.1 1,567,379 - -1.1 1,567,532 + +0.8 1,567,684 - CCNA_01457 0.23 +1.3 1,567,693 - CCNA_01457 0.27 +0.8 1,567,706 + CCNA_01457 0.32 -0.4 1,567,818 + CCNA_01457 0.77 -0.3 1,567,857 - -0.7 1,567,860 + -0.4
Or see this region's nucleotide sequence