Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02065 and CCNA_02066 are separated by 134 nucleotides CCNA_02066 and CCNA_02067 are separated by 71 nucleotides CCNA_02067 and CCNA_02068 are separated by 95 nucleotides
CCNA_02065: CCNA_02065 - acylamino-acid-releasing enzyme, at 2,215,780 to 2,217,882
_02065
CCNA_02066: CCNA_02066 - phytoene/squalene synthase family protein, at 2,218,017 to 2,218,730
_02066
CCNA_02067: CCNA_02067 - conserved membrane spanning protein, at 2,218,802 to 2,219,416
_02067
CCNA_02068: CCNA_02068 - Mth938-like membrane protein, at 2,219,512 to 2,219,865
_02068
Position (kb)
2218
2219 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2217.081 kb on - strand, within CCNA_02065 at 2217.089 kb on + strand, within CCNA_02065 at 2217.099 kb on + strand, within CCNA_02065 at 2217.120 kb on - strand, within CCNA_02065 at 2217.130 kb on + strand, within CCNA_02065 at 2217.130 kb on + strand, within CCNA_02065 at 2217.138 kb on - strand, within CCNA_02065 at 2217.158 kb on + strand, within CCNA_02065 at 2217.229 kb on + strand, within CCNA_02065 at 2217.255 kb on + strand, within CCNA_02065 at 2217.263 kb on - strand, within CCNA_02065 at 2217.319 kb on - strand, within CCNA_02065 at 2217.321 kb on + strand, within CCNA_02065 at 2217.321 kb on + strand, within CCNA_02065 at 2217.345 kb on + strand, within CCNA_02065 at 2217.353 kb on - strand, within CCNA_02065 at 2217.382 kb on + strand, within CCNA_02065 at 2217.389 kb on + strand, within CCNA_02065 at 2217.390 kb on - strand, within CCNA_02065 at 2217.411 kb on - strand, within CCNA_02065 at 2217.455 kb on - strand, within CCNA_02065 at 2217.548 kb on - strand, within CCNA_02065 at 2217.564 kb on + strand, within CCNA_02065 at 2217.616 kb on + strand, within CCNA_02065 at 2217.662 kb on + strand, within CCNA_02065 at 2217.718 kb on - strand at 2217.751 kb on + strand at 2217.762 kb on + strand at 2217.768 kb on + strand at 2217.771 kb on - strand at 2217.774 kb on + strand at 2217.801 kb on + strand at 2217.802 kb on + strand at 2217.810 kb on - strand at 2217.822 kb on + strand at 2217.900 kb on + strand at 2217.908 kb on - strand at 2217.944 kb on - strand at 2217.959 kb on + strand at 2217.975 kb on - strand at 2217.999 kb on + strand at 2218.168 kb on + strand, within CCNA_02066 at 2218.176 kb on - strand, within CCNA_02066 at 2218.176 kb on - strand, within CCNA_02066 at 2218.478 kb on - strand, within CCNA_02066 at 2218.478 kb on - strand, within CCNA_02066 at 2218.478 kb on - strand, within CCNA_02066 at 2218.553 kb on - strand, within CCNA_02066 at 2218.675 kb on - strand at 2218.680 kb on + strand at 2218.726 kb on + strand at 2218.840 kb on + strand at 2218.866 kb on - strand, within CCNA_02067 at 2218.902 kb on + strand, within CCNA_02067 at 2218.904 kb on - strand, within CCNA_02067 at 2218.904 kb on - strand, within CCNA_02067 at 2218.973 kb on - strand, within CCNA_02067 at 2218.997 kb on + strand, within CCNA_02067 at 2219.010 kb on - strand, within CCNA_02067 at 2219.018 kb on + strand, within CCNA_02067 at 2219.018 kb on + strand, within CCNA_02067 at 2219.049 kb on - strand, within CCNA_02067 at 2219.073 kb on - strand, within CCNA_02067 at 2219.075 kb on - strand, within CCNA_02067 at 2219.126 kb on + strand, within CCNA_02067 at 2219.153 kb on - strand, within CCNA_02067 at 2219.249 kb on - strand, within CCNA_02067 at 2219.368 kb on - strand at 2219.528 kb on + strand at 2219.554 kb on + strand, within CCNA_02068 at 2219.562 kb on - strand, within CCNA_02068 at 2219.588 kb on + strand, within CCNA_02068 at 2219.627 kb on - strand, within CCNA_02068 at 2219.654 kb on + strand, within CCNA_02068 at 2219.659 kb on + strand, within CCNA_02068 at 2219.659 kb on + strand, within CCNA_02068 at 2219.724 kb on + strand, within CCNA_02068
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 2,217,081 - CCNA_02065 0.62 -2.8 2,217,089 + CCNA_02065 0.62 +0.0 2,217,099 + CCNA_02065 0.63 +1.2 2,217,120 - CCNA_02065 0.64 -2.7 2,217,130 + CCNA_02065 0.64 +0.2 2,217,130 + CCNA_02065 0.64 +1.2 2,217,138 - CCNA_02065 0.65 +1.0 2,217,158 + CCNA_02065 0.66 -0.1 2,217,229 + CCNA_02065 0.69 -0.1 2,217,255 + CCNA_02065 0.70 +0.0 2,217,263 - CCNA_02065 0.71 +0.8 2,217,319 - CCNA_02065 0.73 +0.4 2,217,321 + CCNA_02065 0.73 +0.3 2,217,321 + CCNA_02065 0.73 -0.1 2,217,345 + CCNA_02065 0.74 +1.8 2,217,353 - CCNA_02065 0.75 -0.3 2,217,382 + CCNA_02065 0.76 +0.5 2,217,389 + CCNA_02065 0.77 +0.3 2,217,390 - CCNA_02065 0.77 +0.2 2,217,411 - CCNA_02065 0.78 -0.2 2,217,455 - CCNA_02065 0.80 -0.6 2,217,548 - CCNA_02065 0.84 -1.3 2,217,564 + CCNA_02065 0.85 -0.8 2,217,616 + CCNA_02065 0.87 +1.1 2,217,662 + CCNA_02065 0.89 -0.6 2,217,718 - -1.2 2,217,751 + +0.7 2,217,762 + +0.5 2,217,768 + +0.1 2,217,771 - -0.9 2,217,774 + +0.6 2,217,801 + -1.5 2,217,802 + -1.5 2,217,810 - -3.2 2,217,822 + +0.8 2,217,900 + +1.5 2,217,908 - +0.6 2,217,944 - +0.4 2,217,959 + +2.3 2,217,975 - +0.2 2,217,999 + -0.2 2,218,168 + CCNA_02066 0.21 -1.7 2,218,176 - CCNA_02066 0.22 -0.1 2,218,176 - CCNA_02066 0.22 +0.4 2,218,478 - CCNA_02066 0.65 -0.7 2,218,478 - CCNA_02066 0.65 -0.3 2,218,478 - CCNA_02066 0.65 +1.0 2,218,553 - CCNA_02066 0.75 -0.8 2,218,675 - -0.3 2,218,680 + -1.6 2,218,726 + +1.3 2,218,840 + +0.9 2,218,866 - CCNA_02067 0.10 +2.1 2,218,902 + CCNA_02067 0.16 -0.3 2,218,904 - CCNA_02067 0.17 +1.8 2,218,904 - CCNA_02067 0.17 -0.0 2,218,973 - CCNA_02067 0.28 +1.5 2,218,997 + CCNA_02067 0.32 -0.4 2,219,010 - CCNA_02067 0.34 +0.7 2,219,018 + CCNA_02067 0.35 -0.4 2,219,018 + CCNA_02067 0.35 -1.0 2,219,049 - CCNA_02067 0.40 +0.8 2,219,073 - CCNA_02067 0.44 +1.5 2,219,075 - CCNA_02067 0.44 +0.8 2,219,126 + CCNA_02067 0.53 -0.7 2,219,153 - CCNA_02067 0.57 +0.3 2,219,249 - CCNA_02067 0.73 +0.7 2,219,368 - +0.9 2,219,528 + -0.2 2,219,554 + CCNA_02068 0.12 -0.2 2,219,562 - CCNA_02068 0.14 +1.3 2,219,588 + CCNA_02068 0.21 +0.7 2,219,627 - CCNA_02068 0.32 -0.5 2,219,654 + CCNA_02068 0.40 +0.1 2,219,659 + CCNA_02068 0.42 -1.0 2,219,659 + CCNA_02068 0.42 -1.4 2,219,724 + CCNA_02068 0.60 -0.8
Or see this region's nucleotide sequence