Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01618 and CCNA_01619 are separated by 2 nucleotides CCNA_01619 and CCNA_01620 are separated by 49 nucleotides CCNA_01620 and CCNA_01621 are separated by 128 nucleotides CCNA_01621 and CCNA_01622 are separated by 101 nucleotides
CCNA_01618: CCNA_01618 - uracil-DNA glycosylase, at 1,739,738 to 1,740,415
_01618
CCNA_01619: CCNA_01619 - NYN domain protein, at 1,740,418 to 1,741,014
_01619
CCNA_01620: CCNA_01620 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase, at 1,741,064 to 1,741,558
_01620
CCNA_01621: CCNA_01621 - DNA-directed RNA polymerase omega chain, at 1,741,687 to 1,742,046
_01621
CCNA_01622: CCNA_01622 - Rsh ppGpp hydrolase-synthetase, at 1,742,148 to 1,744,376
_01622
Position (kb)
1741
1742 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1740.076 kb on + strand, within CCNA_01618 at 1740.159 kb on - strand, within CCNA_01618 at 1740.409 kb on + strand at 1740.411 kb on + strand at 1740.411 kb on + strand at 1740.416 kb on + strand at 1740.416 kb on + strand at 1740.416 kb on + strand at 1740.416 kb on + strand at 1740.424 kb on - strand at 1740.429 kb on - strand at 1740.429 kb on - strand at 1740.449 kb on + strand at 1740.460 kb on + strand at 1740.552 kb on + strand, within CCNA_01619 at 1740.596 kb on + strand, within CCNA_01619 at 1740.626 kb on + strand, within CCNA_01619 at 1740.626 kb on + strand, within CCNA_01619 at 1740.634 kb on - strand, within CCNA_01619 at 1740.656 kb on + strand, within CCNA_01619 at 1740.656 kb on + strand, within CCNA_01619 at 1740.729 kb on - strand, within CCNA_01619 at 1740.761 kb on - strand, within CCNA_01619 at 1740.762 kb on - strand, within CCNA_01619 at 1740.787 kb on - strand, within CCNA_01619 at 1740.789 kb on + strand, within CCNA_01619 at 1740.797 kb on - strand, within CCNA_01619 at 1740.797 kb on - strand, within CCNA_01619 at 1740.880 kb on + strand, within CCNA_01619 at 1740.888 kb on - strand, within CCNA_01619 at 1740.930 kb on + strand, within CCNA_01619 at 1740.945 kb on - strand, within CCNA_01619 at 1740.956 kb on + strand at 1740.984 kb on + strand at 1741.015 kb on + strand at 1741.022 kb on - strand at 1742.035 kb on - strand at 1742.037 kb on + strand at 1742.037 kb on + strand at 1742.040 kb on + strand at 1742.048 kb on - strand at 1742.064 kb on + strand at 1742.072 kb on - strand at 1742.079 kb on - strand at 1742.099 kb on + strand at 1742.107 kb on - strand at 1742.225 kb on + strand at 1742.234 kb on + strand at 1742.234 kb on + strand at 1742.242 kb on + strand at 1742.242 kb on - strand at 1742.242 kb on - strand at 1742.250 kb on - strand at 1742.268 kb on + strand at 1742.270 kb on + strand at 1742.276 kb on - strand at 1742.321 kb on + strand at 1742.329 kb on - strand at 1742.336 kb on + strand at 1742.336 kb on + strand at 1742.384 kb on + strand, within CCNA_01622 at 1742.550 kb on + strand, within CCNA_01622
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 1,740,076 + CCNA_01618 0.50 +1.1 1,740,159 - CCNA_01618 0.62 +1.1 1,740,409 + -1.3 1,740,411 + -1.2 1,740,411 + +0.5 1,740,416 + -1.2 1,740,416 + -0.8 1,740,416 + -0.7 1,740,416 + -1.8 1,740,424 - +1.1 1,740,429 - +0.8 1,740,429 - +1.0 1,740,449 + +1.1 1,740,460 + +0.7 1,740,552 + CCNA_01619 0.22 +1.1 1,740,596 + CCNA_01619 0.30 -0.4 1,740,626 + CCNA_01619 0.35 +1.5 1,740,626 + CCNA_01619 0.35 +0.7 1,740,634 - CCNA_01619 0.36 -0.8 1,740,656 + CCNA_01619 0.40 -1.1 1,740,656 + CCNA_01619 0.40 -0.3 1,740,729 - CCNA_01619 0.52 -1.3 1,740,761 - CCNA_01619 0.57 -0.0 1,740,762 - CCNA_01619 0.58 +0.8 1,740,787 - CCNA_01619 0.62 +0.6 1,740,789 + CCNA_01619 0.62 +0.5 1,740,797 - CCNA_01619 0.63 +1.0 1,740,797 - CCNA_01619 0.63 +0.1 1,740,880 + CCNA_01619 0.77 -0.5 1,740,888 - CCNA_01619 0.79 +0.6 1,740,930 + CCNA_01619 0.86 +1.8 1,740,945 - CCNA_01619 0.88 +0.2 1,740,956 + +0.2 1,740,984 + +0.6 1,741,015 + -0.8 1,741,022 - +0.8 1,742,035 - +1.4 1,742,037 + +1.3 1,742,037 + +0.5 1,742,040 + -0.1 1,742,048 - +0.1 1,742,064 + +0.4 1,742,072 - +1.1 1,742,079 - -1.3 1,742,099 + +0.5 1,742,107 - -1.1 1,742,225 + +0.4 1,742,234 + -2.5 1,742,234 + +0.7 1,742,242 + -0.5 1,742,242 - -2.9 1,742,242 - -2.0 1,742,250 - +0.8 1,742,268 + +0.9 1,742,270 + -0.3 1,742,276 - +1.1 1,742,321 + -0.4 1,742,329 - +0.1 1,742,336 + +1.6 1,742,336 + +0.6 1,742,384 + CCNA_01622 0.11 -0.4 1,742,550 + CCNA_01622 0.18 +2.4
Or see this region's nucleotide sequence