Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00308 and CCNA_00309 are separated by 122 nucleotides CCNA_00309 and CCNA_00310 are separated by 20 nucleotides CCNA_00310 and CCNA_00311 overlap by 4 nucleotides
CCNA_00308: CCNA_00308 - protein tyrosine phosphatase CtpA, at 323,024 to 323,689
_00308
CCNA_00309: CCNA_00309 - hypothetical protein, at 323,812 to 324,045
_00309
CCNA_00310: CCNA_00310 - 3'-phosphoadenosine-5'-phosphosulfate (PAPS) 3'-phosphatase family protein, at 324,066 to 324,845
_00310
CCNA_00311: CCNA_00311 - Zn-dependent protease family protein, at 324,842 to 326,173
_00311
Position (kb)
324
325 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 323.067 kb on - strand at 323.067 kb on - strand at 323.254 kb on + strand, within CCNA_00308 at 323.653 kb on - strand at 323.704 kb on + strand at 323.704 kb on + strand at 323.714 kb on - strand at 323.726 kb on - strand at 323.732 kb on - strand at 323.811 kb on - strand at 323.811 kb on - strand at 324.057 kb on + strand at 324.057 kb on + strand at 324.064 kb on + strand at 324.065 kb on - strand at 324.065 kb on - strand at 324.065 kb on - strand at 324.132 kb on - strand at 324.166 kb on + strand, within CCNA_00310 at 324.174 kb on - strand, within CCNA_00310 at 324.195 kb on - strand, within CCNA_00310 at 324.195 kb on - strand, within CCNA_00310 at 324.302 kb on + strand, within CCNA_00310 at 324.310 kb on - strand, within CCNA_00310 at 324.422 kb on + strand, within CCNA_00310 at 324.431 kb on - strand, within CCNA_00310 at 324.444 kb on + strand, within CCNA_00310 at 324.452 kb on - strand, within CCNA_00310 at 324.461 kb on - strand, within CCNA_00310 at 324.462 kb on + strand, within CCNA_00310 at 324.469 kb on - strand, within CCNA_00310 at 324.475 kb on - strand, within CCNA_00310 at 324.480 kb on + strand, within CCNA_00310 at 324.483 kb on - strand, within CCNA_00310 at 324.486 kb on + strand, within CCNA_00310 at 324.491 kb on + strand, within CCNA_00310 at 324.514 kb on - strand, within CCNA_00310 at 324.529 kb on + strand, within CCNA_00310 at 324.562 kb on + strand, within CCNA_00310 at 324.598 kb on + strand, within CCNA_00310 at 324.612 kb on - strand, within CCNA_00310 at 324.692 kb on + strand, within CCNA_00310 at 324.752 kb on + strand, within CCNA_00310 at 324.782 kb on - strand at 324.799 kb on - strand at 324.825 kb on + strand at 324.830 kb on + strand at 324.838 kb on - strand at 324.838 kb on - strand at 325.004 kb on + strand, within CCNA_00311 at 325.004 kb on + strand, within CCNA_00311 at 325.005 kb on - strand, within CCNA_00311 at 325.076 kb on + strand, within CCNA_00311 at 325.076 kb on + strand, within CCNA_00311 at 325.089 kb on - strand, within CCNA_00311 at 325.150 kb on - strand, within CCNA_00311 at 325.193 kb on + strand, within CCNA_00311 at 325.234 kb on + strand, within CCNA_00311 at 325.256 kb on + strand, within CCNA_00311 at 325.298 kb on + strand, within CCNA_00311 at 325.312 kb on + strand, within CCNA_00311 at 325.320 kb on - strand, within CCNA_00311 at 325.446 kb on - strand, within CCNA_00311 at 325.446 kb on - strand, within CCNA_00311 at 325.521 kb on - strand, within CCNA_00311 at 325.531 kb on - strand, within CCNA_00311 at 325.625 kb on + strand, within CCNA_00311 at 325.638 kb on - strand, within CCNA_00311 at 325.702 kb on + strand, within CCNA_00311 at 325.705 kb on - strand, within CCNA_00311 at 325.758 kb on + strand, within CCNA_00311 at 325.758 kb on + strand, within CCNA_00311 at 325.766 kb on - strand, within CCNA_00311 at 325.766 kb on - strand, within CCNA_00311 at 325.801 kb on + strand, within CCNA_00311 at 325.816 kb on - strand, within CCNA_00311 at 325.840 kb on + strand, within CCNA_00311
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 323,067 - +0.1 323,067 - +0.1 323,254 + CCNA_00308 0.35 +0.9 323,653 - +0.3 323,704 + -1.1 323,704 + +0.1 323,714 - -1.1 323,726 - +0.5 323,732 - +0.3 323,811 - -0.1 323,811 - -0.4 324,057 + -0.6 324,057 + -0.1 324,064 + +0.1 324,065 - +0.1 324,065 - +0.7 324,065 - -0.2 324,132 - -0.1 324,166 + CCNA_00310 0.13 -0.2 324,174 - CCNA_00310 0.14 +0.9 324,195 - CCNA_00310 0.17 +0.1 324,195 - CCNA_00310 0.17 +0.9 324,302 + CCNA_00310 0.30 -0.3 324,310 - CCNA_00310 0.31 -1.8 324,422 + CCNA_00310 0.46 -0.0 324,431 - CCNA_00310 0.47 -0.3 324,444 + CCNA_00310 0.48 +0.9 324,452 - CCNA_00310 0.49 +0.9 324,461 - CCNA_00310 0.51 +0.3 324,462 + CCNA_00310 0.51 +0.4 324,469 - CCNA_00310 0.52 +1.5 324,475 - CCNA_00310 0.52 +0.7 324,480 + CCNA_00310 0.53 -0.6 324,483 - CCNA_00310 0.53 +0.4 324,486 + CCNA_00310 0.54 +0.4 324,491 + CCNA_00310 0.54 +0.4 324,514 - CCNA_00310 0.57 +0.9 324,529 + CCNA_00310 0.59 +0.3 324,562 + CCNA_00310 0.64 +0.6 324,598 + CCNA_00310 0.68 -0.2 324,612 - CCNA_00310 0.70 +1.7 324,692 + CCNA_00310 0.80 -0.4 324,752 + CCNA_00310 0.88 +0.7 324,782 - -0.0 324,799 - -0.7 324,825 + +2.6 324,830 + -1.0 324,838 - -0.3 324,838 - +0.8 325,004 + CCNA_00311 0.12 -0.1 325,004 + CCNA_00311 0.12 +0.2 325,005 - CCNA_00311 0.12 -0.1 325,076 + CCNA_00311 0.18 -0.4 325,076 + CCNA_00311 0.18 +0.0 325,089 - CCNA_00311 0.19 +1.0 325,150 - CCNA_00311 0.23 +0.2 325,193 + CCNA_00311 0.26 +1.6 325,234 + CCNA_00311 0.29 -0.1 325,256 + CCNA_00311 0.31 -0.3 325,298 + CCNA_00311 0.34 +0.3 325,312 + CCNA_00311 0.35 +0.3 325,320 - CCNA_00311 0.36 -0.9 325,446 - CCNA_00311 0.45 -1.5 325,446 - CCNA_00311 0.45 -0.3 325,521 - CCNA_00311 0.51 +0.8 325,531 - CCNA_00311 0.52 +0.1 325,625 + CCNA_00311 0.59 -1.7 325,638 - CCNA_00311 0.60 -0.1 325,702 + CCNA_00311 0.65 -0.5 325,705 - CCNA_00311 0.65 -0.4 325,758 + CCNA_00311 0.69 +0.4 325,758 + CCNA_00311 0.69 -1.5 325,766 - CCNA_00311 0.69 -0.1 325,766 - CCNA_00311 0.69 +2.3 325,801 + CCNA_00311 0.72 -2.3 325,816 - CCNA_00311 0.73 -0.3 325,840 + CCNA_00311 0.75 +0.1
Or see this region's nucleotide sequence