Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00196 and CCNA_00197 are separated by 589 nucleotides CCNA_00197 and CCNA_00198 overlap by 4 nucleotides CCNA_00198 and CCNA_00199 are separated by 130 nucleotides
CCNA_00196: CCNA_00196 - 3-isopropylmalate dehydratase large subunit, at 209,092 to 210,531
_00196
CCNA_00197: CCNA_00197 - LSU ribosomal protein L19P, at 211,121 to 211,522
_00197
CCNA_00198: CCNA_00198 - tRNA (guanine-N1) -methyltransferase, at 211,519 to 212,256
_00198
CCNA_00199: CCNA_00199 - TadG/vWA-family protein, at 212,387 to 214,267
_00199
Position (kb) 211
212 Strain fitness (log2 ratio) -2
-1
0
1
2 at 210.123 kb on + strand, within CCNA_00196 at 210.125 kb on + strand, within CCNA_00196 at 210.162 kb on - strand, within CCNA_00196 at 210.175 kb on + strand, within CCNA_00196 at 210.180 kb on + strand, within CCNA_00196 at 210.180 kb on + strand, within CCNA_00196 at 210.180 kb on + strand, within CCNA_00196 at 210.193 kb on - strand, within CCNA_00196 at 210.214 kb on - strand, within CCNA_00196 at 210.318 kb on + strand, within CCNA_00196 at 210.319 kb on + strand, within CCNA_00196 at 210.322 kb on - strand, within CCNA_00196 at 210.327 kb on - strand, within CCNA_00196 at 210.358 kb on - strand, within CCNA_00196 at 210.369 kb on - strand, within CCNA_00196 at 210.374 kb on - strand, within CCNA_00196 at 210.381 kb on + strand, within CCNA_00196 at 210.381 kb on + strand, within CCNA_00196 at 210.412 kb on + strand at 210.414 kb on + strand at 210.420 kb on - strand at 210.429 kb on + strand at 210.437 kb on - strand at 210.442 kb on + strand at 210.455 kb on - strand at 210.460 kb on - strand at 210.505 kb on - strand at 210.505 kb on - strand at 210.505 kb on - strand at 210.640 kb on - strand at 210.662 kb on - strand at 210.709 kb on + strand at 210.717 kb on - strand at 210.717 kb on - strand at 210.748 kb on - strand at 210.838 kb on + strand at 210.849 kb on + strand at 210.854 kb on + strand at 210.859 kb on + strand at 210.867 kb on - strand at 210.867 kb on - strand at 210.867 kb on - strand at 210.867 kb on - strand at 210.912 kb on + strand at 210.912 kb on + strand at 210.913 kb on + strand at 210.973 kb on + strand at 210.981 kb on - strand at 211.001 kb on + strand at 211.002 kb on + strand at 211.031 kb on + strand at 211.089 kb on - strand at 211.118 kb on + strand at 211.118 kb on + strand at 211.119 kb on - strand at 211.126 kb on - strand at 211.191 kb on - strand, within CCNA_00197 at 211.211 kb on - strand, within CCNA_00197 at 211.561 kb on - strand at 212.288 kb on - strand at 212.288 kb on - strand at 212.293 kb on + strand at 212.295 kb on - strand at 212.295 kb on - strand at 212.316 kb on - strand at 212.325 kb on + strand at 212.377 kb on + strand at 212.382 kb on + strand at 212.382 kb on + strand at 212.382 kb on + strand at 212.398 kb on + strand at 212.409 kb on + strand at 212.415 kb on + strand at 212.423 kb on - strand at 212.448 kb on + strand at 212.464 kb on - strand at 212.492 kb on + strand at 212.500 kb on - strand at 212.520 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 210,123 + CCNA_00196 0.72 +0.6 210,125 + CCNA_00196 0.72 +2.7 210,162 - CCNA_00196 0.74 +0.3 210,175 + CCNA_00196 0.75 +1.7 210,180 + CCNA_00196 0.76 -0.6 210,180 + CCNA_00196 0.76 +1.6 210,180 + CCNA_00196 0.76 +0.3 210,193 - CCNA_00196 0.76 +1.7 210,214 - CCNA_00196 0.78 +1.5 210,318 + CCNA_00196 0.85 -0.9 210,319 + CCNA_00196 0.85 +0.5 210,322 - CCNA_00196 0.85 -0.6 210,327 - CCNA_00196 0.86 -1.2 210,358 - CCNA_00196 0.88 -0.2 210,369 - CCNA_00196 0.89 +0.2 210,374 - CCNA_00196 0.89 +0.6 210,381 + CCNA_00196 0.90 -1.7 210,381 + CCNA_00196 0.90 -1.5 210,412 + -0.1 210,414 + -1.9 210,420 - -1.5 210,429 + -1.5 210,437 - +0.2 210,442 + +0.2 210,455 - -1.0 210,460 - -2.0 210,505 - +0.4 210,505 - -1.1 210,505 - +0.6 210,640 - -1.0 210,662 - +1.4 210,709 + +0.0 210,717 - +0.7 210,717 - +0.4 210,748 - +0.4 210,838 + -0.6 210,849 + +0.2 210,854 + +0.6 210,859 + -2.4 210,867 - +2.2 210,867 - +0.7 210,867 - +0.4 210,867 - -2.0 210,912 + +0.8 210,912 + +0.9 210,913 + +0.3 210,973 + +1.1 210,981 - +0.8 211,001 + +0.3 211,002 + +0.7 211,031 + +0.8 211,089 - +1.3 211,118 + -1.9 211,118 + +0.6 211,119 - +0.9 211,126 - -1.2 211,191 - CCNA_00197 0.17 +1.1 211,211 - CCNA_00197 0.22 -0.6 211,561 - +0.0 212,288 - +0.9 212,288 - -0.1 212,293 + -1.7 212,295 - -0.2 212,295 - -0.1 212,316 - -0.9 212,325 + +0.9 212,377 + -0.3 212,382 + +0.1 212,382 + -0.4 212,382 + -0.3 212,398 + -0.6 212,409 + -0.8 212,415 + -0.4 212,423 - -0.4 212,448 + -0.1 212,464 - -1.6 212,492 + +0.3 212,500 - -2.1 212,520 + +0.3
Or see this region's nucleotide sequence