Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00173 and CCNA_00174 overlap by 14 nucleotides CCNA_00174 and CCNA_00175 are separated by 11 nucleotides CCNA_00175 and CCNA_00176 overlap by 86 nucleotides CCNA_00176 and CCNA_00177 overlap by 32 nucleotides
CCNA_00173: CCNA_00173 - type II secretion pathway protein E, at 188,347 to 189,822
_00173
CCNA_00174: CCNA_00174 - type II secretion pathway protein F, at 189,809 to 191,020
_00174
CCNA_00175: CCNA_00175 - type II secretion pathway protein G, at 191,032 to 191,484
_00175
CCNA_00176: CCNA_00176 - type II secretion pathway protein H, at 191,399 to 191,956
_00176
CCNA_00177: CCNA_00177 - type II secretion pathway protein I, at 191,925 to 192,308
_00177
Position (kb) 189
190
191
192 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 188.837 kb on - strand, within CCNA_00173 at 188.838 kb on + strand, within CCNA_00173 at 188.838 kb on + strand, within CCNA_00173 at 188.838 kb on + strand, within CCNA_00173 at 188.838 kb on + strand, within CCNA_00173 at 188.852 kb on - strand, within CCNA_00173 at 188.852 kb on - strand, within CCNA_00173 at 188.852 kb on - strand, within CCNA_00173 at 188.852 kb on - strand, within CCNA_00173 at 188.881 kb on + strand, within CCNA_00173 at 188.899 kb on + strand, within CCNA_00173 at 188.943 kb on - strand, within CCNA_00173 at 188.991 kb on + strand, within CCNA_00173 at 188.991 kb on + strand, within CCNA_00173 at 189.009 kb on - strand, within CCNA_00173 at 189.011 kb on + strand, within CCNA_00173 at 189.019 kb on - strand, within CCNA_00173 at 189.029 kb on + strand, within CCNA_00173 at 189.034 kb on + strand, within CCNA_00173 at 189.048 kb on + strand, within CCNA_00173 at 189.051 kb on + strand, within CCNA_00173 at 189.077 kb on + strand, within CCNA_00173 at 189.085 kb on - strand, within CCNA_00173 at 189.090 kb on - strand, within CCNA_00173 at 189.103 kb on + strand, within CCNA_00173 at 189.131 kb on - strand, within CCNA_00173 at 189.141 kb on - strand, within CCNA_00173 at 189.156 kb on - strand, within CCNA_00173 at 189.206 kb on + strand, within CCNA_00173 at 189.211 kb on + strand, within CCNA_00173 at 189.219 kb on - strand, within CCNA_00173 at 189.230 kb on - strand, within CCNA_00173 at 189.230 kb on - strand, within CCNA_00173 at 189.230 kb on - strand, within CCNA_00173 at 189.230 kb on - strand, within CCNA_00173 at 189.245 kb on + strand, within CCNA_00173 at 189.264 kb on - strand, within CCNA_00173 at 189.264 kb on - strand, within CCNA_00173 at 189.299 kb on - strand, within CCNA_00173 at 189.323 kb on - strand, within CCNA_00173 at 189.333 kb on - strand, within CCNA_00173 at 189.337 kb on + strand, within CCNA_00173 at 189.345 kb on - strand, within CCNA_00173 at 189.350 kb on - strand, within CCNA_00173 at 189.359 kb on + strand, within CCNA_00173 at 189.367 kb on - strand, within CCNA_00173 at 189.381 kb on + strand, within CCNA_00173 at 189.384 kb on - strand, within CCNA_00173 at 189.384 kb on - strand, within CCNA_00173 at 189.403 kb on + strand, within CCNA_00173 at 189.452 kb on - strand, within CCNA_00173 at 189.490 kb on - strand, within CCNA_00173 at 189.565 kb on + strand, within CCNA_00173 at 189.693 kb on + strand at 189.693 kb on + strand at 189.708 kb on - strand at 189.713 kb on - strand at 189.718 kb on - strand at 189.743 kb on + strand at 189.744 kb on - strand at 189.748 kb on + strand at 189.748 kb on + strand at 189.749 kb on - strand at 189.751 kb on - strand at 189.751 kb on - strand at 189.753 kb on + strand at 189.771 kb on + strand at 189.793 kb on + strand at 189.802 kb on - strand at 189.815 kb on + strand at 189.815 kb on + strand at 189.824 kb on - strand at 189.824 kb on - strand at 189.830 kb on + strand at 189.865 kb on + strand at 189.873 kb on - strand at 189.930 kb on + strand at 189.979 kb on + strand, within CCNA_00174 at 189.987 kb on - strand, within CCNA_00174 at 190.054 kb on + strand, within CCNA_00174 at 190.058 kb on - strand, within CCNA_00174 at 190.066 kb on + strand, within CCNA_00174 at 190.066 kb on + strand, within CCNA_00174 at 190.081 kb on + strand, within CCNA_00174 at 190.098 kb on + strand, within CCNA_00174 at 190.121 kb on + strand, within CCNA_00174 at 190.144 kb on - strand, within CCNA_00174 at 190.151 kb on - strand, within CCNA_00174 at 190.161 kb on + strand, within CCNA_00174 at 190.168 kb on - strand, within CCNA_00174 at 190.174 kb on + strand, within CCNA_00174 at 190.182 kb on - strand, within CCNA_00174 at 190.190 kb on + strand, within CCNA_00174 at 190.195 kb on + strand, within CCNA_00174 at 190.198 kb on - strand, within CCNA_00174 at 190.274 kb on - strand, within CCNA_00174 at 190.293 kb on - strand, within CCNA_00174 at 190.307 kb on + strand, within CCNA_00174 at 190.307 kb on + strand, within CCNA_00174 at 190.307 kb on + strand, within CCNA_00174 at 190.307 kb on + strand, within CCNA_00174 at 190.307 kb on + strand, within CCNA_00174 at 190.307 kb on + strand, within CCNA_00174 at 190.307 kb on + strand, within CCNA_00174 at 190.307 kb on + strand, within CCNA_00174 at 190.307 kb on + strand, within CCNA_00174 at 190.315 kb on - strand, within CCNA_00174 at 190.315 kb on - strand, within CCNA_00174 at 190.315 kb on - strand, within CCNA_00174 at 190.315 kb on - strand, within CCNA_00174 at 190.315 kb on - strand, within CCNA_00174 at 190.330 kb on + strand, within CCNA_00174 at 190.367 kb on + strand, within CCNA_00174 at 190.424 kb on + strand, within CCNA_00174 at 190.450 kb on + strand, within CCNA_00174 at 190.466 kb on + strand, within CCNA_00174 at 190.475 kb on - strand, within CCNA_00174 at 190.513 kb on - strand, within CCNA_00174 at 190.513 kb on - strand, within CCNA_00174 at 190.522 kb on - strand, within CCNA_00174 at 190.597 kb on - strand, within CCNA_00174 at 190.611 kb on - strand, within CCNA_00174 at 190.616 kb on + strand, within CCNA_00174 at 190.624 kb on - strand, within CCNA_00174 at 190.629 kb on - strand, within CCNA_00174 at 190.631 kb on - strand, within CCNA_00174 at 190.670 kb on + strand, within CCNA_00174 at 190.671 kb on + strand, within CCNA_00174 at 190.671 kb on + strand, within CCNA_00174 at 190.671 kb on - strand, within CCNA_00174 at 190.679 kb on - strand, within CCNA_00174 at 190.696 kb on + strand, within CCNA_00174 at 190.702 kb on - strand, within CCNA_00174 at 190.704 kb on - strand, within CCNA_00174 at 190.708 kb on + strand, within CCNA_00174 at 190.714 kb on - strand, within CCNA_00174 at 190.770 kb on - strand, within CCNA_00174 at 190.779 kb on + strand, within CCNA_00174 at 190.787 kb on - strand, within CCNA_00174 at 190.831 kb on - strand, within CCNA_00174 at 190.848 kb on - strand, within CCNA_00174 at 190.848 kb on - strand, within CCNA_00174 at 190.909 kb on + strand at 190.970 kb on - strand at 191.004 kb on + strand at 191.009 kb on + strand at 191.040 kb on + strand at 191.081 kb on + strand, within CCNA_00175 at 191.081 kb on + strand, within CCNA_00175 at 191.089 kb on - strand, within CCNA_00175 at 191.089 kb on - strand, within CCNA_00175 at 191.089 kb on - strand, within CCNA_00175 at 191.099 kb on - strand, within CCNA_00175 at 191.125 kb on - strand, within CCNA_00175 at 191.181 kb on + strand, within CCNA_00175 at 191.189 kb on - strand, within CCNA_00175 at 191.253 kb on + strand, within CCNA_00175 at 191.261 kb on - strand, within CCNA_00175 at 191.268 kb on + strand, within CCNA_00175 at 191.300 kb on + strand, within CCNA_00175 at 191.300 kb on + strand, within CCNA_00175 at 191.300 kb on + strand, within CCNA_00175 at 191.307 kb on - strand, within CCNA_00175 at 191.312 kb on - strand, within CCNA_00175 at 191.312 kb on - strand, within CCNA_00175 at 191.349 kb on + strand, within CCNA_00175 at 191.357 kb on - strand, within CCNA_00175 at 191.374 kb on + strand, within CCNA_00175 at 191.376 kb on + strand, within CCNA_00175 at 191.379 kb on + strand, within CCNA_00175 at 191.452 kb on + strand at 191.485 kb on + strand, within CCNA_00176 at 191.493 kb on - strand, within CCNA_00176 at 191.493 kb on - strand, within CCNA_00176 at 191.494 kb on + strand, within CCNA_00176 at 191.502 kb on - strand, within CCNA_00176 at 191.513 kb on + strand, within CCNA_00176 at 191.518 kb on + strand, within CCNA_00176 at 191.523 kb on + strand, within CCNA_00176 at 191.531 kb on - strand, within CCNA_00176 at 191.539 kb on + strand, within CCNA_00176 at 191.539 kb on + strand, within CCNA_00176 at 191.540 kb on - strand, within CCNA_00176 at 191.547 kb on - strand, within CCNA_00176 at 191.567 kb on + strand, within CCNA_00176 at 191.567 kb on - strand, within CCNA_00176 at 191.579 kb on + strand, within CCNA_00176 at 191.584 kb on + strand, within CCNA_00176 at 191.586 kb on + strand, within CCNA_00176 at 191.586 kb on + strand, within CCNA_00176 at 191.586 kb on + strand, within CCNA_00176 at 191.592 kb on - strand, within CCNA_00176 at 191.592 kb on - strand, within CCNA_00176 at 191.592 kb on - strand, within CCNA_00176 at 191.592 kb on - strand, within CCNA_00176 at 191.592 kb on - strand, within CCNA_00176 at 191.626 kb on - strand, within CCNA_00176 at 191.645 kb on + strand, within CCNA_00176 at 191.695 kb on + strand, within CCNA_00176 at 191.753 kb on + strand, within CCNA_00176 at 191.761 kb on - strand, within CCNA_00176 at 191.761 kb on - strand, within CCNA_00176 at 191.788 kb on + strand, within CCNA_00176 at 191.828 kb on - strand, within CCNA_00176 at 191.828 kb on - strand, within CCNA_00176 at 191.880 kb on + strand, within CCNA_00176 at 191.888 kb on + strand, within CCNA_00176 at 191.962 kb on + strand at 191.969 kb on - strand, within CCNA_00177 at 191.984 kb on + strand, within CCNA_00177 at 192.007 kb on - strand, within CCNA_00177 at 192.020 kb on - strand, within CCNA_00177
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 188,837 - CCNA_00173 0.33 +0.2 188,838 + CCNA_00173 0.33 -0.3 188,838 + CCNA_00173 0.33 -2.8 188,838 + CCNA_00173 0.33 +0.4 188,838 + CCNA_00173 0.33 +0.5 188,852 - CCNA_00173 0.34 +0.8 188,852 - CCNA_00173 0.34 -0.7 188,852 - CCNA_00173 0.34 -0.6 188,852 - CCNA_00173 0.34 +0.1 188,881 + CCNA_00173 0.36 +0.4 188,899 + CCNA_00173 0.37 -0.2 188,943 - CCNA_00173 0.40 +0.0 188,991 + CCNA_00173 0.44 +0.6 188,991 + CCNA_00173 0.44 +0.7 189,009 - CCNA_00173 0.45 -0.1 189,011 + CCNA_00173 0.45 +1.4 189,019 - CCNA_00173 0.46 +0.2 189,029 + CCNA_00173 0.46 +0.4 189,034 + CCNA_00173 0.47 -1.3 189,048 + CCNA_00173 0.47 +0.9 189,051 + CCNA_00173 0.48 -1.7 189,077 + CCNA_00173 0.49 -0.8 189,085 - CCNA_00173 0.50 +0.1 189,090 - CCNA_00173 0.50 +1.1 189,103 + CCNA_00173 0.51 +2.1 189,131 - CCNA_00173 0.53 -0.3 189,141 - CCNA_00173 0.54 -1.0 189,156 - CCNA_00173 0.55 +0.3 189,206 + CCNA_00173 0.58 -1.3 189,211 + CCNA_00173 0.59 -2.2 189,219 - CCNA_00173 0.59 +0.1 189,230 - CCNA_00173 0.60 -0.2 189,230 - CCNA_00173 0.60 -0.9 189,230 - CCNA_00173 0.60 +0.1 189,230 - CCNA_00173 0.60 -0.6 189,245 + CCNA_00173 0.61 -0.5 189,264 - CCNA_00173 0.62 -0.2 189,264 - CCNA_00173 0.62 +2.8 189,299 - CCNA_00173 0.64 -0.1 189,323 - CCNA_00173 0.66 -0.2 189,333 - CCNA_00173 0.67 -0.6 189,337 + CCNA_00173 0.67 +0.0 189,345 - CCNA_00173 0.68 -1.4 189,350 - CCNA_00173 0.68 -0.4 189,359 + CCNA_00173 0.69 -0.5 189,367 - CCNA_00173 0.69 +0.6 189,381 + CCNA_00173 0.70 +1.3 189,384 - CCNA_00173 0.70 -0.2 189,384 - CCNA_00173 0.70 -0.3 189,403 + CCNA_00173 0.72 +1.7 189,452 - CCNA_00173 0.75 +0.4 189,490 - CCNA_00173 0.77 -2.3 189,565 + CCNA_00173 0.83 +0.1 189,693 + +0.0 189,693 + -1.1 189,708 - +0.4 189,713 - -0.2 189,718 - +0.5 189,743 + +0.0 189,744 - +0.7 189,748 + +0.2 189,748 + -0.2 189,749 - -2.4 189,751 - -0.1 189,751 - +0.5 189,753 + -0.3 189,771 + +1.4 189,793 + -0.7 189,802 - +2.1 189,815 + -0.5 189,815 + -0.1 189,824 - -0.0 189,824 - +0.2 189,830 + -1.1 189,865 + +0.8 189,873 - +0.0 189,930 + -0.1 189,979 + CCNA_00174 0.14 -0.2 189,987 - CCNA_00174 0.15 -1.7 190,054 + CCNA_00174 0.20 -0.2 190,058 - CCNA_00174 0.21 -0.7 190,066 + CCNA_00174 0.21 -0.3 190,066 + CCNA_00174 0.21 -0.3 190,081 + CCNA_00174 0.22 -0.0 190,098 + CCNA_00174 0.24 +0.9 190,121 + CCNA_00174 0.26 +2.2 190,144 - CCNA_00174 0.28 -0.4 190,151 - CCNA_00174 0.28 -1.4 190,161 + CCNA_00174 0.29 +0.4 190,168 - CCNA_00174 0.30 -0.8 190,174 + CCNA_00174 0.30 -0.5 190,182 - CCNA_00174 0.31 -1.2 190,190 + CCNA_00174 0.31 +1.6 190,195 + CCNA_00174 0.32 +2.0 190,198 - CCNA_00174 0.32 -1.4 190,274 - CCNA_00174 0.38 -0.6 190,293 - CCNA_00174 0.40 -1.5 190,307 + CCNA_00174 0.41 -0.2 190,307 + CCNA_00174 0.41 -1.0 190,307 + CCNA_00174 0.41 -0.5 190,307 + CCNA_00174 0.41 -1.3 190,307 + CCNA_00174 0.41 -0.1 190,307 + CCNA_00174 0.41 -2.1 190,307 + CCNA_00174 0.41 -1.4 190,307 + CCNA_00174 0.41 +0.3 190,307 + CCNA_00174 0.41 +0.1 190,315 - CCNA_00174 0.42 -1.0 190,315 - CCNA_00174 0.42 +0.6 190,315 - CCNA_00174 0.42 -1.6 190,315 - CCNA_00174 0.42 -0.3 190,315 - CCNA_00174 0.42 -3.5 190,330 + CCNA_00174 0.43 -0.0 190,367 + CCNA_00174 0.46 -0.1 190,424 + CCNA_00174 0.51 +0.3 190,450 + CCNA_00174 0.53 +0.2 190,466 + CCNA_00174 0.54 +1.1 190,475 - CCNA_00174 0.55 -2.1 190,513 - CCNA_00174 0.58 +0.4 190,513 - CCNA_00174 0.58 -2.8 190,522 - CCNA_00174 0.59 +0.2 190,597 - CCNA_00174 0.65 -2.0 190,611 - CCNA_00174 0.66 -0.1 190,616 + CCNA_00174 0.67 +0.2 190,624 - CCNA_00174 0.67 +0.1 190,629 - CCNA_00174 0.68 -0.1 190,631 - CCNA_00174 0.68 -0.4 190,670 + CCNA_00174 0.71 -0.3 190,671 + CCNA_00174 0.71 -1.2 190,671 + CCNA_00174 0.71 -0.2 190,671 - CCNA_00174 0.71 -2.6 190,679 - CCNA_00174 0.72 -0.5 190,696 + CCNA_00174 0.73 -0.6 190,702 - CCNA_00174 0.74 -1.2 190,704 - CCNA_00174 0.74 -0.5 190,708 + CCNA_00174 0.74 -1.0 190,714 - CCNA_00174 0.75 -0.6 190,770 - CCNA_00174 0.79 +1.4 190,779 + CCNA_00174 0.80 +0.1 190,787 - CCNA_00174 0.81 -0.4 190,831 - CCNA_00174 0.84 -0.7 190,848 - CCNA_00174 0.86 -0.2 190,848 - CCNA_00174 0.86 -2.0 190,909 + +0.0 190,970 - +0.2 191,004 + -0.8 191,009 + +0.2 191,040 + +0.0 191,081 + CCNA_00175 0.11 -0.5 191,081 + CCNA_00175 0.11 -1.1 191,089 - CCNA_00175 0.13 -1.7 191,089 - CCNA_00175 0.13 +0.4 191,089 - CCNA_00175 0.13 -0.9 191,099 - CCNA_00175 0.15 +0.1 191,125 - CCNA_00175 0.21 -0.2 191,181 + CCNA_00175 0.33 -0.3 191,189 - CCNA_00175 0.35 -0.7 191,253 + CCNA_00175 0.49 -1.2 191,261 - CCNA_00175 0.51 -0.8 191,268 + CCNA_00175 0.52 -0.1 191,300 + CCNA_00175 0.59 +1.5 191,300 + CCNA_00175 0.59 +1.1 191,300 + CCNA_00175 0.59 +0.1 191,307 - CCNA_00175 0.61 -1.3 191,312 - CCNA_00175 0.62 -1.7 191,312 - CCNA_00175 0.62 +0.0 191,349 + CCNA_00175 0.70 +0.1 191,357 - CCNA_00175 0.72 +0.1 191,374 + CCNA_00175 0.75 +0.1 191,376 + CCNA_00175 0.76 -0.0 191,379 + CCNA_00175 0.77 -1.3 191,452 + +0.9 191,485 + CCNA_00176 0.15 +0.2 191,493 - CCNA_00176 0.17 +0.8 191,493 - CCNA_00176 0.17 +0.8 191,494 + CCNA_00176 0.17 -0.2 191,502 - CCNA_00176 0.18 -0.3 191,513 + CCNA_00176 0.20 -0.4 191,518 + CCNA_00176 0.21 +1.0 191,523 + CCNA_00176 0.22 +0.3 191,531 - CCNA_00176 0.24 +1.3 191,539 + CCNA_00176 0.25 -1.8 191,539 + CCNA_00176 0.25 +0.1 191,540 - CCNA_00176 0.25 +0.3 191,547 - CCNA_00176 0.27 -0.4 191,567 + CCNA_00176 0.30 +0.4 191,567 - CCNA_00176 0.30 +0.9 191,579 + CCNA_00176 0.32 +0.6 191,584 + CCNA_00176 0.33 -0.0 191,586 + CCNA_00176 0.34 +0.8 191,586 + CCNA_00176 0.34 -0.7 191,586 + CCNA_00176 0.34 +0.5 191,592 - CCNA_00176 0.35 -0.2 191,592 - CCNA_00176 0.35 -0.6 191,592 - CCNA_00176 0.35 +0.8 191,592 - CCNA_00176 0.35 +0.5 191,592 - CCNA_00176 0.35 -0.6 191,626 - CCNA_00176 0.41 +0.4 191,645 + CCNA_00176 0.44 -1.5 191,695 + CCNA_00176 0.53 +0.6 191,753 + CCNA_00176 0.63 -1.4 191,761 - CCNA_00176 0.65 -1.2 191,761 - CCNA_00176 0.65 +0.0 191,788 + CCNA_00176 0.70 -0.6 191,828 - CCNA_00176 0.77 +0.9 191,828 - CCNA_00176 0.77 -0.3 191,880 + CCNA_00176 0.86 -1.5 191,888 + CCNA_00176 0.88 -0.6 191,962 + -0.3 191,969 - CCNA_00177 0.11 -0.7 191,984 + CCNA_00177 0.15 -0.7 192,007 - CCNA_00177 0.21 -0.1 192,020 - CCNA_00177 0.25 -0.8
Or see this region's nucleotide sequence