Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS10450 and ABID97_RS10455 overlap by 4 nucleotides ABID97_RS10455 and ABID97_RS10460 are separated by 35 nucleotides ABID97_RS10460 and ABID97_RS10465 are separated by 149 nucleotides
ABID97_RS10450: ABID97_RS10450 - aromatic ring-hydroxylating dioxygenase subunit alpha, at 2,172,347 to 2,173,453
_RS10450
ABID97_RS10455: ABID97_RS10455 - GntR family transcriptional regulator, at 2,173,450 to 2,174,226
_RS10455
ABID97_RS10460: ABID97_RS10460 - PDR/VanB family oxidoreductase, at 2,174,262 to 2,175,248
_RS10460
ABID97_RS10465: ABID97_RS10465 - ABC transporter substrate-binding protein, at 2,175,398 to 2,176,573
_RS10465
Position (kb)
2174
2175
2176 Strain fitness (log2 ratio)
-2
-1
0
1 at 2173.331 kb on + strand, within ABID97_RS10450 at 2173.331 kb on + strand, within ABID97_RS10450 at 2173.332 kb on - strand, within ABID97_RS10450 at 2174.370 kb on + strand, within ABID97_RS10460 at 2174.371 kb on - strand, within ABID97_RS10460 at 2174.371 kb on - strand, within ABID97_RS10460 at 2174.436 kb on + strand, within ABID97_RS10460 at 2174.844 kb on + strand, within ABID97_RS10460 at 2175.319 kb on + strand at 2175.563 kb on + strand, within ABID97_RS10465 at 2175.563 kb on + strand, within ABID97_RS10465 at 2175.564 kb on - strand, within ABID97_RS10465 at 2175.809 kb on + strand, within ABID97_RS10465 at 2175.810 kb on - strand, within ABID97_RS10465
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 2,173,331 + ABID97_RS10450 0.89 -0.0 2,173,331 + ABID97_RS10450 0.89 -0.4 2,173,332 - ABID97_RS10450 0.89 -0.4 2,174,370 + ABID97_RS10460 0.11 -0.3 2,174,371 - ABID97_RS10460 0.11 +0.2 2,174,371 - ABID97_RS10460 0.11 +0.5 2,174,436 + ABID97_RS10460 0.18 +0.6 2,174,844 + ABID97_RS10460 0.59 +0.2 2,175,319 + -2.1 2,175,563 + ABID97_RS10465 0.14 +0.6 2,175,563 + ABID97_RS10465 0.14 -0.4 2,175,564 - ABID97_RS10465 0.14 -0.3 2,175,809 + ABID97_RS10465 0.35 -0.5 2,175,810 - ABID97_RS10465 0.35 +0.1
Or see this region's nucleotide sequence