Strain Fitness in Escherichia coli BL21 around ECD_01730

Experiment: NoPhageControl

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01727 and ECD_01728 are separated by 86 nucleotidesECD_01728 and ECD_01729 overlap by 35 nucleotidesECD_01729 and ECD_01730 are separated by 235 nucleotidesECD_01730 and ECD_01731 are separated by 116 nucleotides ECD_01727: ECD_01727 - CDP-alcohol phosphatidyltransferase family inner membrane protein, at 1,785,159 to 1,785,779 _01727 ECD_01728: ECD_01728 - CTP pyrophosphohydrolase; also hydrolyzes 2-hydroxy-dATP, 8-hydroxy-dGTP, 5-hydroxy-CTP, dCTP and 5-methyl-dCTP, at 1,785,866 to 1,786,273 _01728 ECD_01729: ECD_01729 - DUF1496 family protein, at 1,786,239 to 1,786,511 _01729 ECD_01730: ECD_01730 - glutamate dehydrogenase, NADP-specific, at 1,786,747 to 1,788,090 _01730 ECD_01731: ECD_01731 - inner membrane protein, at 1,788,207 to 1,789,247 _01731 Position (kb) 1786 1787 1788 1789Strain fitness (log2 ratio) -1 0 1at 1785.795 kb on - strandat 1786.670 kb on + strandat 1786.731 kb on + strandat 1786.759 kb on + strandat 1787.632 kb on + strand, within ECD_01730at 1787.880 kb on + strand, within ECD_01730at 1788.095 kb on + strandat 1788.153 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction NoPhageControl
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1,785,795 - +0.5
1,786,670 + +0.3
1,786,731 + +0.8
1,786,759 + -0.3
1,787,632 + ECD_01730 0.66 +0.1
1,787,880 + ECD_01730 0.84 -0.8
1,788,095 + +0.1
1,788,153 + +0.9

Or see this region's nucleotide sequence