Strain Fitness in Escherichia coli BL21 around ECD_01086

Experiment: NoPhageControl

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01084 and ECD_01085 are separated by 51 nucleotidesECD_01085 and ECD_01086 are separated by 80 nucleotidesECD_01086 and ECD_01087 are separated by 67 nucleotides ECD_01084: ECD_01084 - DUF177 family protein, at 1,149,361 to 1,149,882 _01084 ECD_01085: ECD_01085 - 50S ribosomal subunit protein L32, at 1,149,934 to 1,150,107 _01085 ECD_01086: ECD_01086 - putative phosphate acyltransferase, at 1,150,188 to 1,151,258 _01086 ECD_01087: ECD_01087 - 3-oxoacyl-[acyl-carrier-protein] synthase III, at 1,151,326 to 1,152,279 _01087 Position (kb) 1150 1151 1152Strain fitness (log2 ratio) -2 -1 0 1at 1149.209 kb on + strandat 1149.377 kb on + strandat 1149.402 kb on + strandat 1149.432 kb on + strand, within ECD_01084at 1149.436 kb on + strand, within ECD_01084at 1149.436 kb on + strand, within ECD_01084at 1149.436 kb on + strand, within ECD_01084at 1149.613 kb on + strand, within ECD_01084at 1149.613 kb on + strand, within ECD_01084at 1149.622 kb on + strand, within ECD_01084at 1149.622 kb on + strand, within ECD_01084at 1149.880 kb on + strandat 1150.210 kb on + strandat 1151.298 kb on + strandat 1151.298 kb on + strandat 1151.298 kb on + strandat 1151.300 kb on + strandat 1151.300 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction NoPhageControl
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1,149,209 + +0.2
1,149,377 + -0.5
1,149,402 + -2.2
1,149,432 + ECD_01084 0.14 -0.5
1,149,436 + ECD_01084 0.14 -1.3
1,149,436 + ECD_01084 0.14 -0.0
1,149,436 + ECD_01084 0.14 +0.1
1,149,613 + ECD_01084 0.48 -0.1
1,149,613 + ECD_01084 0.48 +0.8
1,149,622 + ECD_01084 0.50 -1.1
1,149,622 + ECD_01084 0.50 -0.5
1,149,880 + -0.1
1,150,210 + -0.0
1,151,298 + +0.7
1,151,298 + +0.1
1,151,298 + -0.0
1,151,300 + -0.2
1,151,300 + +0.1

Or see this region's nucleotide sequence