Strain Fitness in Escherichia coli BL21 around ECD_00115

Experiment: NoPhageControl

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00114 and ECD_00115 are separated by 324 nucleotidesECD_00115 and ECD_00116 are separated by 70 nucleotides ECD_00114: ECD_00114 - pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2, at 128,500 to 130,392 _00114 ECD_00115: ECD_00115 - dihydrolipoyl dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase, at 130,717 to 132,141 _00115 ECD_00116: ECD_00116 - DUF3300 family protein, at 132,212 to 134,065 _00116 Position (kb) 130 131 132 133Strain fitness (log2 ratio) -2 -1 0 1at 130.391 kb on - strandat 132.140 kb on - strandat 132.170 kb on + strandat 132.170 kb on + strandat 132.293 kb on - strandat 132.298 kb on + strandat 132.739 kb on - strand, within ECD_00116at 132.748 kb on - strand, within ECD_00116at 132.763 kb on + strand, within ECD_00116at 132.951 kb on + strand, within ECD_00116at 132.970 kb on - strand, within ECD_00116at 132.970 kb on - strand, within ECD_00116at 132.995 kb on - strand, within ECD_00116at 133.006 kb on + strand, within ECD_00116at 133.006 kb on + strand, within ECD_00116at 133.113 kb on + strand, within ECD_00116at 133.113 kb on + strand, within ECD_00116at 133.114 kb on - strand, within ECD_00116

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Per-strain Table

Position Strand Gene LocusTag Fraction NoPhageControl
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130,391 - -1.1
132,140 - -1.3
132,170 + -0.3
132,170 + -0.7
132,293 - +0.8
132,298 + +0.2
132,739 - ECD_00116 0.28 -0.2
132,748 - ECD_00116 0.29 +0.1
132,763 + ECD_00116 0.30 +0.3
132,951 + ECD_00116 0.40 -2.6
132,970 - ECD_00116 0.41 -0.0
132,970 - ECD_00116 0.41 -2.1
132,995 - ECD_00116 0.42 +0.9
133,006 + ECD_00116 0.43 -0.5
133,006 + ECD_00116 0.43 -1.1
133,113 + ECD_00116 0.49 +0.1
133,113 + ECD_00116 0.49 +0.5
133,114 - ECD_00116 0.49 -0.4

Or see this region's nucleotide sequence