Experiment: m.b. Paraquat dichloride 0.00390625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_0873 and B158DRAFT_0874 are separated by 76 nucleotides B158DRAFT_0874 and B158DRAFT_0875 overlap by 4 nucleotides B158DRAFT_0875 and B158DRAFT_0876 are separated by 107 nucleotides B158DRAFT_0876 and B158DRAFT_0877 are separated by 422 nucleotides
B158DRAFT_0873: B158DRAFT_0873 - DNA adenine methylase Dam, at 888,996 to 889,829
_0873
B158DRAFT_0874: B158DRAFT_0874 - Protein of unknown function (DUF2970)., at 889,906 to 890,190
_0874
B158DRAFT_0875: B158DRAFT_0875 - hypothetical protein, at 890,187 to 890,501
_0875
B158DRAFT_0876: B158DRAFT_0876 - 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase (EC 2.5.1.54), at 890,609 to 891,988
_0876
B158DRAFT_0877: B158DRAFT_0877 - DNA replication and repair protein RadA, at 892,411 to 893,778
_0877
Position (kb) 890
891
892 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 889.780 kb on + strand at 889.793 kb on - strand at 889.793 kb on - strand at 889.798 kb on - strand at 889.831 kb on - strand at 889.835 kb on + strand at 889.845 kb on - strand at 889.884 kb on + strand at 889.884 kb on + strand at 889.892 kb on - strand at 889.892 kb on - strand at 889.892 kb on - strand at 889.911 kb on + strand at 889.919 kb on - strand at 889.926 kb on + strand at 890.310 kb on + strand, within B158DRAFT_0875 at 890.396 kb on - strand, within B158DRAFT_0875 at 890.396 kb on - strand, within B158DRAFT_0875 at 890.396 kb on - strand, within B158DRAFT_0875 at 890.396 kb on - strand, within B158DRAFT_0875 at 890.396 kb on - strand, within B158DRAFT_0875 at 890.398 kb on - strand, within B158DRAFT_0875 at 890.406 kb on + strand, within B158DRAFT_0875 at 890.406 kb on + strand, within B158DRAFT_0875 at 890.406 kb on + strand, within B158DRAFT_0875 at 890.406 kb on + strand, within B158DRAFT_0875 at 890.414 kb on - strand, within B158DRAFT_0875 at 890.414 kb on - strand, within B158DRAFT_0875 at 890.424 kb on + strand, within B158DRAFT_0875 at 890.424 kb on + strand, within B158DRAFT_0875 at 890.424 kb on + strand, within B158DRAFT_0875 at 890.432 kb on - strand, within B158DRAFT_0875 at 890.446 kb on - strand, within B158DRAFT_0875 at 890.446 kb on - strand, within B158DRAFT_0875 at 890.547 kb on + strand at 890.552 kb on + strand at 890.555 kb on - strand at 890.706 kb on - strand at 890.723 kb on + strand at 890.723 kb on + strand at 890.723 kb on + strand at 890.723 kb on + strand at 890.723 kb on + strand at 890.723 kb on + strand at 890.730 kb on + strand at 890.730 kb on + strand at 890.730 kb on + strand at 890.731 kb on - strand at 890.731 kb on - strand at 890.731 kb on - strand at 890.738 kb on - strand at 890.755 kb on + strand, within B158DRAFT_0876 at 890.828 kb on + strand, within B158DRAFT_0876 at 890.829 kb on - strand, within B158DRAFT_0876 at 890.836 kb on - strand, within B158DRAFT_0876 at 890.876 kb on - strand, within B158DRAFT_0876 at 890.902 kb on + strand, within B158DRAFT_0876 at 890.902 kb on + strand, within B158DRAFT_0876 at 890.902 kb on + strand, within B158DRAFT_0876 at 890.902 kb on + strand, within B158DRAFT_0876 at 890.902 kb on + strand, within B158DRAFT_0876 at 890.902 kb on + strand, within B158DRAFT_0876 at 890.902 kb on + strand, within B158DRAFT_0876 at 890.910 kb on - strand, within B158DRAFT_0876 at 890.910 kb on - strand, within B158DRAFT_0876 at 890.910 kb on - strand, within B158DRAFT_0876 at 890.910 kb on - strand, within B158DRAFT_0876 at 890.942 kb on + strand, within B158DRAFT_0876 at 890.945 kb on + strand, within B158DRAFT_0876 at 890.952 kb on + strand, within B158DRAFT_0876 at 890.967 kb on - strand, within B158DRAFT_0876 at 890.976 kb on + strand, within B158DRAFT_0876 at 890.976 kb on + strand, within B158DRAFT_0876 at 890.976 kb on + strand, within B158DRAFT_0876 at 890.976 kb on + strand, within B158DRAFT_0876 at 890.976 kb on + strand, within B158DRAFT_0876 at 890.976 kb on + strand, within B158DRAFT_0876 at 890.976 kb on + strand, within B158DRAFT_0876 at 890.976 kb on + strand, within B158DRAFT_0876 at 890.984 kb on - strand, within B158DRAFT_0876 at 890.984 kb on - strand, within B158DRAFT_0876 at 890.996 kb on + strand, within B158DRAFT_0876 at 890.996 kb on + strand, within B158DRAFT_0876 at 891.004 kb on - strand, within B158DRAFT_0876 at 891.008 kb on + strand, within B158DRAFT_0876 at 891.034 kb on + strand, within B158DRAFT_0876 at 891.089 kb on + strand, within B158DRAFT_0876 at 891.090 kb on - strand, within B158DRAFT_0876 at 891.123 kb on - strand, within B158DRAFT_0876 at 891.167 kb on + strand, within B158DRAFT_0876 at 891.167 kb on + strand, within B158DRAFT_0876 at 891.167 kb on + strand, within B158DRAFT_0876 at 891.167 kb on + strand, within B158DRAFT_0876 at 891.167 kb on + strand, within B158DRAFT_0876 at 891.167 kb on + strand, within B158DRAFT_0876 at 891.169 kb on + strand, within B158DRAFT_0876 at 891.175 kb on - strand, within B158DRAFT_0876 at 891.175 kb on - strand, within B158DRAFT_0876 at 891.175 kb on - strand, within B158DRAFT_0876 at 891.175 kb on - strand, within B158DRAFT_0876 at 891.175 kb on - strand, within B158DRAFT_0876 at 891.177 kb on + strand, within B158DRAFT_0876 at 891.187 kb on + strand, within B158DRAFT_0876 at 891.187 kb on + strand, within B158DRAFT_0876 at 891.187 kb on + strand, within B158DRAFT_0876 at 891.193 kb on + strand, within B158DRAFT_0876 at 891.193 kb on - strand, within B158DRAFT_0876 at 891.245 kb on + strand, within B158DRAFT_0876 at 891.245 kb on + strand, within B158DRAFT_0876 at 891.245 kb on + strand, within B158DRAFT_0876 at 891.296 kb on - strand, within B158DRAFT_0876 at 891.303 kb on - strand, within B158DRAFT_0876 at 891.303 kb on - strand, within B158DRAFT_0876 at 891.312 kb on + strand, within B158DRAFT_0876 at 891.320 kb on - strand, within B158DRAFT_0876 at 891.327 kb on + strand, within B158DRAFT_0876 at 891.335 kb on - strand, within B158DRAFT_0876 at 891.335 kb on - strand, within B158DRAFT_0876 at 891.335 kb on - strand, within B158DRAFT_0876 at 891.335 kb on - strand, within B158DRAFT_0876 at 891.367 kb on + strand, within B158DRAFT_0876 at 891.367 kb on + strand, within B158DRAFT_0876 at 891.367 kb on + strand, within B158DRAFT_0876 at 891.375 kb on - strand, within B158DRAFT_0876 at 891.432 kb on + strand, within B158DRAFT_0876 at 891.437 kb on + strand, within B158DRAFT_0876 at 891.440 kb on - strand, within B158DRAFT_0876 at 891.456 kb on + strand, within B158DRAFT_0876 at 891.457 kb on - strand, within B158DRAFT_0876 at 891.506 kb on + strand, within B158DRAFT_0876 at 891.509 kb on - strand, within B158DRAFT_0876 at 891.509 kb on - strand, within B158DRAFT_0876 at 891.534 kb on - strand, within B158DRAFT_0876 at 891.629 kb on + strand, within B158DRAFT_0876 at 891.637 kb on - strand, within B158DRAFT_0876 at 891.638 kb on + strand, within B158DRAFT_0876 at 891.638 kb on + strand, within B158DRAFT_0876 at 891.646 kb on - strand, within B158DRAFT_0876 at 891.655 kb on + strand, within B158DRAFT_0876 at 891.673 kb on + strand, within B158DRAFT_0876 at 891.700 kb on + strand, within B158DRAFT_0876 at 891.700 kb on + strand, within B158DRAFT_0876 at 891.700 kb on + strand, within B158DRAFT_0876 at 891.700 kb on + strand, within B158DRAFT_0876 at 891.700 kb on + strand, within B158DRAFT_0876 at 891.700 kb on + strand, within B158DRAFT_0876 at 891.700 kb on + strand, within B158DRAFT_0876 at 891.700 kb on + strand, within B158DRAFT_0876 at 891.708 kb on - strand, within B158DRAFT_0876 at 891.708 kb on - strand, within B158DRAFT_0876 at 891.708 kb on - strand, within B158DRAFT_0876 at 891.708 kb on - strand, within B158DRAFT_0876 at 891.708 kb on - strand, within B158DRAFT_0876 at 891.708 kb on - strand, within B158DRAFT_0876 at 891.708 kb on - strand, within B158DRAFT_0876 at 891.716 kb on - strand, within B158DRAFT_0876 at 891.726 kb on - strand, within B158DRAFT_0876 at 891.788 kb on + strand, within B158DRAFT_0876 at 891.788 kb on + strand, within B158DRAFT_0876 at 891.794 kb on + strand, within B158DRAFT_0876 at 891.796 kb on - strand, within B158DRAFT_0876 at 891.844 kb on - strand, within B158DRAFT_0876 at 891.899 kb on + strand at 891.899 kb on + strand at 891.944 kb on - strand at 891.953 kb on + strand at 891.953 kb on + strand at 891.961 kb on - strand at 891.962 kb on + strand at 891.963 kb on + strand at 891.970 kb on - strand at 891.980 kb on - strand at 892.028 kb on - strand at 892.182 kb on + strand at 892.200 kb on - strand at 892.210 kb on - strand at 892.303 kb on + strand at 892.307 kb on + strand at 892.360 kb on - strand at 892.390 kb on - strand at 892.469 kb on + strand at 892.469 kb on + strand at 892.474 kb on + strand at 892.474 kb on + strand at 892.474 kb on + strand at 892.474 kb on + strand at 892.477 kb on - strand at 892.477 kb on - strand at 892.477 kb on - strand at 892.487 kb on - strand at 892.546 kb on + strand at 892.546 kb on + strand at 892.554 kb on - strand, within B158DRAFT_0877 at 892.576 kb on + strand, within B158DRAFT_0877 at 892.583 kb on + strand, within B158DRAFT_0877 at 892.584 kb on - strand, within B158DRAFT_0877 at 892.584 kb on - strand, within B158DRAFT_0877 at 892.589 kb on - strand, within B158DRAFT_0877 at 892.591 kb on - strand, within B158DRAFT_0877 at 892.593 kb on + strand, within B158DRAFT_0877 at 892.607 kb on + strand, within B158DRAFT_0877 at 892.607 kb on + strand, within B158DRAFT_0877 at 892.607 kb on + strand, within B158DRAFT_0877 at 892.607 kb on + strand, within B158DRAFT_0877 at 892.607 kb on + strand, within B158DRAFT_0877 at 892.615 kb on - strand, within B158DRAFT_0877 at 892.615 kb on - strand, within B158DRAFT_0877 at 892.615 kb on - strand, within B158DRAFT_0877 at 892.615 kb on - strand, within B158DRAFT_0877 at 892.616 kb on + strand, within B158DRAFT_0877 at 892.637 kb on + strand, within B158DRAFT_0877 at 892.645 kb on - strand, within B158DRAFT_0877 at 892.645 kb on - strand, within B158DRAFT_0877 at 892.663 kb on + strand, within B158DRAFT_0877 at 892.676 kb on + strand, within B158DRAFT_0877 at 892.748 kb on - strand, within B158DRAFT_0877 at 892.800 kb on - strand, within B158DRAFT_0877 at 892.810 kb on - strand, within B158DRAFT_0877 at 892.837 kb on + strand, within B158DRAFT_0877 at 892.921 kb on - strand, within B158DRAFT_0877 at 892.960 kb on + strand, within B158DRAFT_0877 at 892.972 kb on + strand, within B158DRAFT_0877 at 892.980 kb on - strand, within B158DRAFT_0877 at 892.980 kb on - strand, within B158DRAFT_0877 at 892.982 kb on + strand, within B158DRAFT_0877
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Paraquat dichloride 0.00390625 mg/ml remove 889,780 + +4.6 889,793 - +4.8 889,793 - +4.3 889,798 - +3.3 889,831 - -0.1 889,835 + +0.6 889,845 - -0.6 889,884 + -0.1 889,884 + -0.4 889,892 - +0.4 889,892 - +0.2 889,892 - -1.1 889,911 + +1.7 889,919 - -0.3 889,926 + -0.0 890,310 + B158DRAFT_0875 0.39 -0.4 890,396 - B158DRAFT_0875 0.66 +0.8 890,396 - B158DRAFT_0875 0.66 +1.3 890,396 - B158DRAFT_0875 0.66 +0.7 890,396 - B158DRAFT_0875 0.66 -0.5 890,396 - B158DRAFT_0875 0.66 -0.4 890,398 - B158DRAFT_0875 0.67 -0.9 890,406 + B158DRAFT_0875 0.70 -0.9 890,406 + B158DRAFT_0875 0.70 +0.8 890,406 + B158DRAFT_0875 0.70 -0.1 890,406 + B158DRAFT_0875 0.70 -1.2 890,414 - B158DRAFT_0875 0.72 -1.1 890,414 - B158DRAFT_0875 0.72 -0.3 890,424 + B158DRAFT_0875 0.75 -0.7 890,424 + B158DRAFT_0875 0.75 -2.8 890,424 + B158DRAFT_0875 0.75 +0.3 890,432 - B158DRAFT_0875 0.78 -0.1 890,446 - B158DRAFT_0875 0.82 -0.8 890,446 - B158DRAFT_0875 0.82 -0.1 890,547 + -0.3 890,552 + -0.3 890,555 - -1.5 890,706 - -0.2 890,723 + +0.2 890,723 + -0.1 890,723 + +0.5 890,723 + +0.2 890,723 + -0.5 890,723 + +0.2 890,730 + -0.0 890,730 + -0.0 890,730 + -1.0 890,731 - +2.1 890,731 - -1.0 890,731 - +0.5 890,738 - +0.5 890,755 + B158DRAFT_0876 0.11 -0.1 890,828 + B158DRAFT_0876 0.16 -0.1 890,829 - B158DRAFT_0876 0.16 -0.5 890,836 - B158DRAFT_0876 0.16 -0.2 890,876 - B158DRAFT_0876 0.19 +1.1 890,902 + B158DRAFT_0876 0.21 -1.2 890,902 + B158DRAFT_0876 0.21 +0.6 890,902 + B158DRAFT_0876 0.21 -1.4 890,902 + B158DRAFT_0876 0.21 -0.4 890,902 + B158DRAFT_0876 0.21 -0.6 890,902 + B158DRAFT_0876 0.21 -0.6 890,902 + B158DRAFT_0876 0.21 +0.5 890,910 - B158DRAFT_0876 0.22 +0.5 890,910 - B158DRAFT_0876 0.22 -0.3 890,910 - B158DRAFT_0876 0.22 -0.2 890,910 - B158DRAFT_0876 0.22 +1.2 890,942 + B158DRAFT_0876 0.24 -1.6 890,945 + B158DRAFT_0876 0.24 +0.0 890,952 + B158DRAFT_0876 0.25 -0.4 890,967 - B158DRAFT_0876 0.26 -0.3 890,976 + B158DRAFT_0876 0.27 +0.2 890,976 + B158DRAFT_0876 0.27 -0.2 890,976 + B158DRAFT_0876 0.27 +1.2 890,976 + B158DRAFT_0876 0.27 -0.8 890,976 + B158DRAFT_0876 0.27 -0.2 890,976 + B158DRAFT_0876 0.27 -0.9 890,976 + B158DRAFT_0876 0.27 +2.7 890,976 + B158DRAFT_0876 0.27 -0.2 890,984 - B158DRAFT_0876 0.27 -0.2 890,984 - B158DRAFT_0876 0.27 +0.2 890,996 + B158DRAFT_0876 0.28 -0.6 890,996 + B158DRAFT_0876 0.28 +2.7 891,004 - B158DRAFT_0876 0.29 +0.5 891,008 + B158DRAFT_0876 0.29 -1.0 891,034 + B158DRAFT_0876 0.31 -0.2 891,089 + B158DRAFT_0876 0.35 +0.1 891,090 - B158DRAFT_0876 0.35 +0.0 891,123 - B158DRAFT_0876 0.37 -0.3 891,167 + B158DRAFT_0876 0.40 -0.1 891,167 + B158DRAFT_0876 0.40 +2.0 891,167 + B158DRAFT_0876 0.40 -0.1 891,167 + B158DRAFT_0876 0.40 +0.8 891,167 + B158DRAFT_0876 0.40 +0.2 891,167 + B158DRAFT_0876 0.40 +0.6 891,169 + B158DRAFT_0876 0.41 -0.8 891,175 - B158DRAFT_0876 0.41 -0.2 891,175 - B158DRAFT_0876 0.41 -0.4 891,175 - B158DRAFT_0876 0.41 +0.6 891,175 - B158DRAFT_0876 0.41 -0.1 891,175 - B158DRAFT_0876 0.41 -0.3 891,177 + B158DRAFT_0876 0.41 -0.2 891,187 + B158DRAFT_0876 0.42 -0.5 891,187 + B158DRAFT_0876 0.42 -0.2 891,187 + B158DRAFT_0876 0.42 -0.4 891,193 + B158DRAFT_0876 0.42 +0.4 891,193 - B158DRAFT_0876 0.42 -0.4 891,245 + B158DRAFT_0876 0.46 +0.0 891,245 + B158DRAFT_0876 0.46 -0.1 891,245 + B158DRAFT_0876 0.46 -0.0 891,296 - B158DRAFT_0876 0.50 -0.9 891,303 - B158DRAFT_0876 0.50 +0.4 891,303 - B158DRAFT_0876 0.50 -0.8 891,312 + B158DRAFT_0876 0.51 -0.1 891,320 - B158DRAFT_0876 0.52 -2.3 891,327 + B158DRAFT_0876 0.52 -0.1 891,335 - B158DRAFT_0876 0.53 -0.6 891,335 - B158DRAFT_0876 0.53 -0.7 891,335 - B158DRAFT_0876 0.53 +0.1 891,335 - B158DRAFT_0876 0.53 +4.1 891,367 + B158DRAFT_0876 0.55 -0.2 891,367 + B158DRAFT_0876 0.55 +0.4 891,367 + B158DRAFT_0876 0.55 +0.3 891,375 - B158DRAFT_0876 0.56 -0.3 891,432 + B158DRAFT_0876 0.60 +4.6 891,437 + B158DRAFT_0876 0.60 -0.0 891,440 - B158DRAFT_0876 0.60 +1.5 891,456 + B158DRAFT_0876 0.61 +0.7 891,457 - B158DRAFT_0876 0.61 -0.1 891,506 + B158DRAFT_0876 0.65 +0.3 891,509 - B158DRAFT_0876 0.65 +0.1 891,509 - B158DRAFT_0876 0.65 +0.2 891,534 - B158DRAFT_0876 0.67 +0.1 891,629 + B158DRAFT_0876 0.74 -0.2 891,637 - B158DRAFT_0876 0.74 -0.2 891,638 + B158DRAFT_0876 0.75 -0.5 891,638 + B158DRAFT_0876 0.75 -1.3 891,646 - B158DRAFT_0876 0.75 -0.3 891,655 + B158DRAFT_0876 0.76 +2.3 891,673 + B158DRAFT_0876 0.77 -0.6 891,700 + B158DRAFT_0876 0.79 +0.1 891,700 + B158DRAFT_0876 0.79 -0.2 891,700 + B158DRAFT_0876 0.79 -1.0 891,700 + B158DRAFT_0876 0.79 +0.3 891,700 + B158DRAFT_0876 0.79 -0.5 891,700 + B158DRAFT_0876 0.79 -0.3 891,700 + B158DRAFT_0876 0.79 +0.0 891,700 + B158DRAFT_0876 0.79 +0.4 891,708 - B158DRAFT_0876 0.80 +0.1 891,708 - B158DRAFT_0876 0.80 -1.3 891,708 - B158DRAFT_0876 0.80 -0.3 891,708 - B158DRAFT_0876 0.80 +0.5 891,708 - B158DRAFT_0876 0.80 +2.6 891,708 - B158DRAFT_0876 0.80 -1.6 891,708 - B158DRAFT_0876 0.80 +1.5 891,716 - B158DRAFT_0876 0.80 +0.6 891,726 - B158DRAFT_0876 0.81 -0.0 891,788 + B158DRAFT_0876 0.85 -0.3 891,788 + B158DRAFT_0876 0.85 -0.4 891,794 + B158DRAFT_0876 0.86 +0.5 891,796 - B158DRAFT_0876 0.86 +0.2 891,844 - B158DRAFT_0876 0.89 -0.8 891,899 + -0.4 891,899 + +0.3 891,944 - +0.9 891,953 + +0.3 891,953 + -0.1 891,961 - -1.3 891,962 + -0.5 891,963 + -0.2 891,970 - -0.4 891,980 - -0.3 892,028 - -0.4 892,182 + -2.7 892,200 - -0.0 892,210 - -0.6 892,303 + +0.3 892,307 + -0.7 892,360 - -1.1 892,390 - -0.4 892,469 + -0.5 892,469 + -0.4 892,474 + -0.2 892,474 + -0.7 892,474 + -0.6 892,474 + -0.4 892,477 - +0.1 892,477 - +0.1 892,477 - +2.2 892,487 - +0.5 892,546 + -0.5 892,546 + +0.1 892,554 - B158DRAFT_0877 0.10 -1.1 892,576 + B158DRAFT_0877 0.12 +0.0 892,583 + B158DRAFT_0877 0.13 -2.4 892,584 - B158DRAFT_0877 0.13 -0.1 892,584 - B158DRAFT_0877 0.13 +0.2 892,589 - B158DRAFT_0877 0.13 +0.1 892,591 - B158DRAFT_0877 0.13 -0.3 892,593 + B158DRAFT_0877 0.13 -0.0 892,607 + B158DRAFT_0877 0.14 -0.4 892,607 + B158DRAFT_0877 0.14 -0.6 892,607 + B158DRAFT_0877 0.14 +0.0 892,607 + B158DRAFT_0877 0.14 -1.0 892,607 + B158DRAFT_0877 0.14 -0.5 892,615 - B158DRAFT_0877 0.15 -0.8 892,615 - B158DRAFT_0877 0.15 -0.1 892,615 - B158DRAFT_0877 0.15 -0.3 892,615 - B158DRAFT_0877 0.15 +0.5 892,616 + B158DRAFT_0877 0.15 -1.0 892,637 + B158DRAFT_0877 0.17 +0.7 892,645 - B158DRAFT_0877 0.17 -2.1 892,645 - B158DRAFT_0877 0.17 -0.1 892,663 + B158DRAFT_0877 0.18 -0.1 892,676 + B158DRAFT_0877 0.19 +0.3 892,748 - B158DRAFT_0877 0.25 -0.2 892,800 - B158DRAFT_0877 0.28 +0.7 892,810 - B158DRAFT_0877 0.29 -0.1 892,837 + B158DRAFT_0877 0.31 -0.3 892,921 - B158DRAFT_0877 0.37 -0.5 892,960 + B158DRAFT_0877 0.40 -0.6 892,972 + B158DRAFT_0877 0.41 +0.5 892,980 - B158DRAFT_0877 0.42 -0.0 892,980 - B158DRAFT_0877 0.42 +2.1 892,982 + B158DRAFT_0877 0.42 -0.1
Or see this region's nucleotide sequence