Experiment: m.b. methylglyoxal 0.005 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_1475 and B158DRAFT_1476 overlap by 4 nucleotides B158DRAFT_1476 and B158DRAFT_1477 overlap by 1 nucleotides B158DRAFT_1477 and B158DRAFT_1478 are separated by 419 nucleotides B158DRAFT_1478 and B158DRAFT_1479 are separated by 122 nucleotides
B158DRAFT_1475: B158DRAFT_1475 - Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes, at 1,526,699 to 1,528,417
_1475
B158DRAFT_1476: B158DRAFT_1476 - Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit, at 1,528,414 to 1,529,394
_1476
B158DRAFT_1477: B158DRAFT_1477 - pyruvate dehydrogenase E1 component, alpha subunit, at 1,529,394 to 1,530,485
_1477
B158DRAFT_1478: B158DRAFT_1478 - hypothetical protein, at 1,530,905 to 1,531,288
_1478
B158DRAFT_1479: B158DRAFT_1479 - aminopeptidase N, Escherichia coli type, at 1,531,411 to 1,534,077
_1479
Position (kb)
1529
1530
1531 Strain fitness (log2 ratio)
-1
0
1 at 1529.421 kb on - strand at 1530.172 kb on + strand, within B158DRAFT_1477 at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.534 kb on - strand at 1530.704 kb on - strand at 1530.704 kb on - strand at 1530.777 kb on + strand at 1530.785 kb on - strand at 1530.958 kb on + strand, within B158DRAFT_1478 at 1530.966 kb on - strand, within B158DRAFT_1478 at 1530.968 kb on + strand, within B158DRAFT_1478 at 1530.968 kb on + strand, within B158DRAFT_1478 at 1530.982 kb on + strand, within B158DRAFT_1478 at 1530.990 kb on - strand, within B158DRAFT_1478 at 1531.004 kb on + strand, within B158DRAFT_1478 at 1531.004 kb on + strand, within B158DRAFT_1478 at 1531.004 kb on + strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.045 kb on + strand, within B158DRAFT_1478 at 1531.087 kb on + strand, within B158DRAFT_1478 at 1531.108 kb on + strand, within B158DRAFT_1478 at 1531.148 kb on + strand, within B158DRAFT_1478 at 1531.159 kb on + strand, within B158DRAFT_1478 at 1531.159 kb on + strand, within B158DRAFT_1478 at 1531.167 kb on - strand, within B158DRAFT_1478 at 1531.167 kb on - strand, within B158DRAFT_1478 at 1531.167 kb on - strand, within B158DRAFT_1478 at 1531.167 kb on - strand, within B158DRAFT_1478 at 1531.190 kb on - strand, within B158DRAFT_1478 at 1531.190 kb on - strand, within B158DRAFT_1478 at 1531.194 kb on + strand, within B158DRAFT_1478 at 1531.194 kb on + strand, within B158DRAFT_1478 at 1531.194 kb on + strand, within B158DRAFT_1478 at 1531.194 kb on + strand, within B158DRAFT_1478 at 1531.198 kb on + strand, within B158DRAFT_1478 at 1531.202 kb on - strand, within B158DRAFT_1478 at 1531.202 kb on - strand, within B158DRAFT_1478 at 1531.202 kb on - strand, within B158DRAFT_1478 at 1531.202 kb on - strand, within B158DRAFT_1478 at 1531.203 kb on + strand, within B158DRAFT_1478 at 1531.217 kb on + strand, within B158DRAFT_1478 at 1531.217 kb on + strand, within B158DRAFT_1478 at 1531.271 kb on + strand at 1531.278 kb on + strand at 1531.286 kb on - strand at 1531.290 kb on + strand at 1531.441 kb on - strand at 1531.450 kb on + strand at 1531.458 kb on - strand at 1531.458 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. methylglyoxal 0.005 vol% remove 1,529,421 - -0.3 1,530,172 + B158DRAFT_1477 0.71 -1.2 1,530,503 - +1.3 1,530,503 - +0.6 1,530,503 - -0.4 1,530,503 - +0.1 1,530,503 - +0.6 1,530,503 - -0.4 1,530,503 - +0.5 1,530,503 - +0.8 1,530,503 - +0.2 1,530,503 - +0.3 1,530,534 - +0.4 1,530,704 - +0.1 1,530,704 - -0.0 1,530,777 + +0.2 1,530,785 - -0.1 1,530,958 + B158DRAFT_1478 0.14 +0.9 1,530,966 - B158DRAFT_1478 0.16 +0.9 1,530,968 + B158DRAFT_1478 0.16 +0.2 1,530,968 + B158DRAFT_1478 0.16 +0.3 1,530,982 + B158DRAFT_1478 0.20 +0.2 1,530,990 - B158DRAFT_1478 0.22 -0.4 1,531,004 + B158DRAFT_1478 0.26 +0.8 1,531,004 + B158DRAFT_1478 0.26 +1.1 1,531,004 + B158DRAFT_1478 0.26 +0.8 1,531,012 - B158DRAFT_1478 0.28 +0.1 1,531,012 - B158DRAFT_1478 0.28 +0.9 1,531,012 - B158DRAFT_1478 0.28 +0.0 1,531,012 - B158DRAFT_1478 0.28 +0.9 1,531,012 - B158DRAFT_1478 0.28 -0.7 1,531,012 - B158DRAFT_1478 0.28 -0.2 1,531,012 - B158DRAFT_1478 0.28 -0.1 1,531,012 - B158DRAFT_1478 0.28 +0.5 1,531,012 - B158DRAFT_1478 0.28 +0.7 1,531,012 - B158DRAFT_1478 0.28 +0.8 1,531,012 - B158DRAFT_1478 0.28 +0.6 1,531,045 + B158DRAFT_1478 0.36 +0.1 1,531,087 + B158DRAFT_1478 0.47 +0.6 1,531,108 + B158DRAFT_1478 0.53 -0.1 1,531,148 + B158DRAFT_1478 0.63 -0.5 1,531,159 + B158DRAFT_1478 0.66 +0.0 1,531,159 + B158DRAFT_1478 0.66 +0.3 1,531,167 - B158DRAFT_1478 0.68 +0.4 1,531,167 - B158DRAFT_1478 0.68 -0.2 1,531,167 - B158DRAFT_1478 0.68 -0.2 1,531,167 - B158DRAFT_1478 0.68 +0.6 1,531,190 - B158DRAFT_1478 0.74 +0.8 1,531,190 - B158DRAFT_1478 0.74 -0.1 1,531,194 + B158DRAFT_1478 0.75 -0.8 1,531,194 + B158DRAFT_1478 0.75 +1.4 1,531,194 + B158DRAFT_1478 0.75 +0.5 1,531,194 + B158DRAFT_1478 0.75 -0.1 1,531,198 + B158DRAFT_1478 0.76 +0.5 1,531,202 - B158DRAFT_1478 0.77 +0.5 1,531,202 - B158DRAFT_1478 0.77 +0.7 1,531,202 - B158DRAFT_1478 0.77 +1.0 1,531,202 - B158DRAFT_1478 0.77 +0.3 1,531,203 + B158DRAFT_1478 0.78 +1.5 1,531,217 + B158DRAFT_1478 0.81 +0.2 1,531,217 + B158DRAFT_1478 0.81 +0.3 1,531,271 + +0.9 1,531,278 + -0.4 1,531,286 - +0.5 1,531,290 + +0.4 1,531,441 - +0.2 1,531,450 + +0.8 1,531,458 - +0.4 1,531,458 - +0.2
Or see this region's nucleotide sequence