Experiment: m.b. methylglyoxal 0.005 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_1114 and B158DRAFT_1115 are separated by 6 nucleotides B158DRAFT_1115 and B158DRAFT_1116 are separated by 5 nucleotides B158DRAFT_1116 and B158DRAFT_1117 are separated by 47 nucleotides
B158DRAFT_1114: B158DRAFT_1114 - S-adenosylhomocysteine hydrolase, at 1,151,052 to 1,152,107
_1114
B158DRAFT_1115: B158DRAFT_1115 - Predicted esterase of the alpha-beta hydrolase superfamily, at 1,152,114 to 1,153,268
_1115
B158DRAFT_1116: B158DRAFT_1116 - Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains), at 1,153,274 to 1,153,825
_1116
B158DRAFT_1117: B158DRAFT_1117 - prolyl-tRNA synthetase (EC 6.1.1.15), at 1,153,873 to 1,155,588
_1117
Position (kb)
1152
1153
1154 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1151.480 kb on + strand, within B158DRAFT_1114 at 1151.480 kb on + strand, within B158DRAFT_1114 at 1151.488 kb on - strand, within B158DRAFT_1114 at 1151.538 kb on + strand, within B158DRAFT_1114 at 1151.546 kb on - strand, within B158DRAFT_1114 at 1151.546 kb on - strand, within B158DRAFT_1114 at 1151.556 kb on - strand, within B158DRAFT_1114 at 1151.618 kb on + strand, within B158DRAFT_1114 at 1151.638 kb on - strand, within B158DRAFT_1114 at 1151.655 kb on - strand, within B158DRAFT_1114 at 1151.655 kb on - strand, within B158DRAFT_1114 at 1151.728 kb on - strand, within B158DRAFT_1114 at 1151.730 kb on + strand, within B158DRAFT_1114 at 1151.773 kb on + strand, within B158DRAFT_1114 at 1151.827 kb on + strand, within B158DRAFT_1114 at 1151.835 kb on - strand, within B158DRAFT_1114 at 1151.914 kb on - strand, within B158DRAFT_1114 at 1151.928 kb on + strand, within B158DRAFT_1114 at 1151.928 kb on + strand, within B158DRAFT_1114 at 1151.928 kb on + strand, within B158DRAFT_1114 at 1151.928 kb on + strand, within B158DRAFT_1114 at 1151.928 kb on + strand, within B158DRAFT_1114 at 1151.949 kb on - strand, within B158DRAFT_1114 at 1152.009 kb on + strand at 1152.042 kb on + strand at 1152.059 kb on - strand at 1152.065 kb on + strand at 1152.070 kb on + strand at 1152.113 kb on + strand at 1152.170 kb on + strand at 1152.217 kb on - strand at 1152.254 kb on - strand, within B158DRAFT_1115 at 1152.341 kb on + strand, within B158DRAFT_1115 at 1152.341 kb on + strand, within B158DRAFT_1115 at 1152.341 kb on + strand, within B158DRAFT_1115 at 1152.341 kb on + strand, within B158DRAFT_1115 at 1152.375 kb on - strand, within B158DRAFT_1115 at 1152.375 kb on - strand, within B158DRAFT_1115 at 1152.377 kb on + strand, within B158DRAFT_1115 at 1152.403 kb on + strand, within B158DRAFT_1115 at 1152.431 kb on - strand, within B158DRAFT_1115 at 1152.473 kb on - strand, within B158DRAFT_1115 at 1152.558 kb on + strand, within B158DRAFT_1115 at 1152.566 kb on - strand, within B158DRAFT_1115 at 1152.568 kb on - strand, within B158DRAFT_1115 at 1152.607 kb on + strand, within B158DRAFT_1115 at 1152.609 kb on + strand, within B158DRAFT_1115 at 1152.609 kb on + strand, within B158DRAFT_1115 at 1152.609 kb on + strand, within B158DRAFT_1115 at 1152.609 kb on + strand, within B158DRAFT_1115 at 1152.609 kb on + strand, within B158DRAFT_1115 at 1152.609 kb on + strand, within B158DRAFT_1115 at 1152.617 kb on - strand, within B158DRAFT_1115 at 1152.617 kb on - strand, within B158DRAFT_1115 at 1152.617 kb on - strand, within B158DRAFT_1115 at 1152.617 kb on - strand, within B158DRAFT_1115 at 1152.617 kb on - strand, within B158DRAFT_1115 at 1152.678 kb on - strand, within B158DRAFT_1115 at 1152.710 kb on - strand, within B158DRAFT_1115 at 1152.725 kb on - strand, within B158DRAFT_1115 at 1152.731 kb on - strand, within B158DRAFT_1115 at 1152.731 kb on - strand, within B158DRAFT_1115 at 1152.731 kb on - strand, within B158DRAFT_1115 at 1152.768 kb on + strand, within B158DRAFT_1115 at 1152.776 kb on - strand, within B158DRAFT_1115 at 1152.805 kb on + strand, within B158DRAFT_1115 at 1152.813 kb on - strand, within B158DRAFT_1115 at 1152.821 kb on + strand, within B158DRAFT_1115 at 1152.821 kb on - strand, within B158DRAFT_1115 at 1152.911 kb on + strand, within B158DRAFT_1115 at 1153.025 kb on + strand, within B158DRAFT_1115 at 1153.025 kb on + strand, within B158DRAFT_1115 at 1153.025 kb on + strand, within B158DRAFT_1115 at 1153.052 kb on + strand, within B158DRAFT_1115 at 1153.060 kb on - strand, within B158DRAFT_1115 at 1153.070 kb on - strand, within B158DRAFT_1115 at 1153.100 kb on + strand, within B158DRAFT_1115 at 1153.186 kb on + strand at 1153.231 kb on + strand at 1153.239 kb on - strand at 1153.247 kb on + strand at 1153.249 kb on + strand at 1153.262 kb on - strand at 1153.310 kb on - strand at 1153.310 kb on - strand at 1153.333 kb on + strand, within B158DRAFT_1116 at 1153.367 kb on - strand, within B158DRAFT_1116 at 1153.371 kb on - strand, within B158DRAFT_1116 at 1153.412 kb on + strand, within B158DRAFT_1116 at 1153.444 kb on - strand, within B158DRAFT_1116 at 1153.448 kb on + strand, within B158DRAFT_1116 at 1153.456 kb on - strand, within B158DRAFT_1116 at 1153.482 kb on + strand, within B158DRAFT_1116 at 1153.482 kb on + strand, within B158DRAFT_1116 at 1153.482 kb on + strand, within B158DRAFT_1116 at 1153.482 kb on + strand, within B158DRAFT_1116 at 1153.490 kb on - strand, within B158DRAFT_1116 at 1153.506 kb on - strand, within B158DRAFT_1116 at 1153.559 kb on + strand, within B158DRAFT_1116 at 1153.568 kb on - strand, within B158DRAFT_1116 at 1153.641 kb on + strand, within B158DRAFT_1116 at 1153.649 kb on - strand, within B158DRAFT_1116 at 1153.649 kb on - strand, within B158DRAFT_1116 at 1153.660 kb on + strand, within B158DRAFT_1116 at 1153.691 kb on + strand, within B158DRAFT_1116 at 1153.783 kb on - strand at 1153.800 kb on + strand at 1153.829 kb on + strand at 1153.829 kb on + strand at 1153.836 kb on + strand at 1153.837 kb on - strand at 1153.844 kb on - strand at 1153.846 kb on + strand at 1153.846 kb on + strand at 1153.854 kb on - strand at 1153.856 kb on + strand at 1153.864 kb on - strand at 1153.888 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. methylglyoxal 0.005 vol% remove 1,151,480 + B158DRAFT_1114 0.41 -0.5 1,151,480 + B158DRAFT_1114 0.41 +0.1 1,151,488 - B158DRAFT_1114 0.41 -0.4 1,151,538 + B158DRAFT_1114 0.46 -3.9 1,151,546 - B158DRAFT_1114 0.47 -0.1 1,151,546 - B158DRAFT_1114 0.47 -0.7 1,151,556 - B158DRAFT_1114 0.48 -0.3 1,151,618 + B158DRAFT_1114 0.54 +1.5 1,151,638 - B158DRAFT_1114 0.55 +0.2 1,151,655 - B158DRAFT_1114 0.57 -0.4 1,151,655 - B158DRAFT_1114 0.57 +0.3 1,151,728 - B158DRAFT_1114 0.64 -0.1 1,151,730 + B158DRAFT_1114 0.64 +0.4 1,151,773 + B158DRAFT_1114 0.68 +0.0 1,151,827 + B158DRAFT_1114 0.73 -3.2 1,151,835 - B158DRAFT_1114 0.74 +1.3 1,151,914 - B158DRAFT_1114 0.82 -0.6 1,151,928 + B158DRAFT_1114 0.83 -0.2 1,151,928 + B158DRAFT_1114 0.83 -3.8 1,151,928 + B158DRAFT_1114 0.83 -0.3 1,151,928 + B158DRAFT_1114 0.83 -1.9 1,151,928 + B158DRAFT_1114 0.83 -0.9 1,151,949 - B158DRAFT_1114 0.85 +0.3 1,152,009 + +0.6 1,152,042 + +0.4 1,152,059 - -0.0 1,152,065 + -0.4 1,152,070 + +0.2 1,152,113 + +0.7 1,152,170 + -0.4 1,152,217 - +0.3 1,152,254 - B158DRAFT_1115 0.12 +0.3 1,152,341 + B158DRAFT_1115 0.20 +1.3 1,152,341 + B158DRAFT_1115 0.20 -0.4 1,152,341 + B158DRAFT_1115 0.20 +0.6 1,152,341 + B158DRAFT_1115 0.20 -1.5 1,152,375 - B158DRAFT_1115 0.23 +0.6 1,152,375 - B158DRAFT_1115 0.23 +0.3 1,152,377 + B158DRAFT_1115 0.23 +0.5 1,152,403 + B158DRAFT_1115 0.25 -1.7 1,152,431 - B158DRAFT_1115 0.27 +0.6 1,152,473 - B158DRAFT_1115 0.31 +0.8 1,152,558 + B158DRAFT_1115 0.38 -0.2 1,152,566 - B158DRAFT_1115 0.39 +1.0 1,152,568 - B158DRAFT_1115 0.39 -0.4 1,152,607 + B158DRAFT_1115 0.43 +0.5 1,152,609 + B158DRAFT_1115 0.43 +1.4 1,152,609 + B158DRAFT_1115 0.43 +0.3 1,152,609 + B158DRAFT_1115 0.43 +1.2 1,152,609 + B158DRAFT_1115 0.43 +0.2 1,152,609 + B158DRAFT_1115 0.43 -0.6 1,152,609 + B158DRAFT_1115 0.43 -1.0 1,152,617 - B158DRAFT_1115 0.44 +0.3 1,152,617 - B158DRAFT_1115 0.44 +0.6 1,152,617 - B158DRAFT_1115 0.44 -3.5 1,152,617 - B158DRAFT_1115 0.44 +1.0 1,152,617 - B158DRAFT_1115 0.44 +0.7 1,152,678 - B158DRAFT_1115 0.49 +0.4 1,152,710 - B158DRAFT_1115 0.52 +0.6 1,152,725 - B158DRAFT_1115 0.53 +0.0 1,152,731 - B158DRAFT_1115 0.53 -0.1 1,152,731 - B158DRAFT_1115 0.53 +0.7 1,152,731 - B158DRAFT_1115 0.53 +1.6 1,152,768 + B158DRAFT_1115 0.57 -0.2 1,152,776 - B158DRAFT_1115 0.57 +0.2 1,152,805 + B158DRAFT_1115 0.60 +0.0 1,152,813 - B158DRAFT_1115 0.61 +0.1 1,152,821 + B158DRAFT_1115 0.61 +1.4 1,152,821 - B158DRAFT_1115 0.61 -0.0 1,152,911 + B158DRAFT_1115 0.69 -0.3 1,153,025 + B158DRAFT_1115 0.79 +0.3 1,153,025 + B158DRAFT_1115 0.79 -1.0 1,153,025 + B158DRAFT_1115 0.79 -0.2 1,153,052 + B158DRAFT_1115 0.81 -1.9 1,153,060 - B158DRAFT_1115 0.82 +0.3 1,153,070 - B158DRAFT_1115 0.83 +0.4 1,153,100 + B158DRAFT_1115 0.85 -1.2 1,153,186 + +1.0 1,153,231 + +1.4 1,153,239 - -0.2 1,153,247 + +0.2 1,153,249 + -0.2 1,153,262 - -1.1 1,153,310 - -1.0 1,153,310 - +1.7 1,153,333 + B158DRAFT_1116 0.11 +0.7 1,153,367 - B158DRAFT_1116 0.17 +0.2 1,153,371 - B158DRAFT_1116 0.18 -0.2 1,153,412 + B158DRAFT_1116 0.25 +0.2 1,153,444 - B158DRAFT_1116 0.31 +0.2 1,153,448 + B158DRAFT_1116 0.32 -3.3 1,153,456 - B158DRAFT_1116 0.33 +0.2 1,153,482 + B158DRAFT_1116 0.38 -2.7 1,153,482 + B158DRAFT_1116 0.38 -0.2 1,153,482 + B158DRAFT_1116 0.38 -0.4 1,153,482 + B158DRAFT_1116 0.38 +0.6 1,153,490 - B158DRAFT_1116 0.39 +0.8 1,153,506 - B158DRAFT_1116 0.42 -0.4 1,153,559 + B158DRAFT_1116 0.52 -0.9 1,153,568 - B158DRAFT_1116 0.53 -0.0 1,153,641 + B158DRAFT_1116 0.66 +0.8 1,153,649 - B158DRAFT_1116 0.68 +0.7 1,153,649 - B158DRAFT_1116 0.68 +0.6 1,153,660 + B158DRAFT_1116 0.70 +0.1 1,153,691 + B158DRAFT_1116 0.76 -1.5 1,153,783 - +0.4 1,153,800 + -0.4 1,153,829 + -0.4 1,153,829 + -0.3 1,153,836 + -0.6 1,153,837 - +0.9 1,153,844 - +0.9 1,153,846 + -0.1 1,153,846 + +0.9 1,153,854 - +0.8 1,153,856 + -1.0 1,153,864 - +0.6 1,153,888 + -0.7
Or see this region's nucleotide sequence