Experiment: m.b. Gentamicin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2539 and B158DRAFT_2540 are separated by 60 nucleotides B158DRAFT_2540 and B158DRAFT_2541 are separated by 57 nucleotides B158DRAFT_2541 and B158DRAFT_2542 are separated by 183 nucleotides
B158DRAFT_2539: B158DRAFT_2539 - hypothetical protein, at 730,027 to 730,596
_2539
B158DRAFT_2540: B158DRAFT_2540 - hypothetical protein, at 730,657 to 731,133
_2540
B158DRAFT_2541: B158DRAFT_2541 - Protein of unknown function (DUF3579)., at 731,191 to 731,472
_2541
B158DRAFT_2542: B158DRAFT_2542 - Phosphatidylserine synthase, at 731,656 to 732,459
_2542
Position (kb)
731
732 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 730.308 kb on + strand, within B158DRAFT_2539 at 730.311 kb on - strand, within B158DRAFT_2539 at 730.311 kb on - strand, within B158DRAFT_2539 at 730.343 kb on + strand, within B158DRAFT_2539 at 730.357 kb on + strand, within B158DRAFT_2539 at 730.357 kb on + strand, within B158DRAFT_2539 at 730.357 kb on + strand, within B158DRAFT_2539 at 730.365 kb on - strand, within B158DRAFT_2539 at 730.365 kb on - strand, within B158DRAFT_2539 at 730.367 kb on + strand, within B158DRAFT_2539 at 730.377 kb on - strand, within B158DRAFT_2539 at 730.377 kb on - strand, within B158DRAFT_2539 at 730.398 kb on + strand, within B158DRAFT_2539 at 730.442 kb on - strand, within B158DRAFT_2539 at 730.442 kb on - strand, within B158DRAFT_2539 at 730.515 kb on + strand, within B158DRAFT_2539 at 730.541 kb on + strand at 730.555 kb on + strand at 730.719 kb on + strand, within B158DRAFT_2540 at 730.719 kb on + strand, within B158DRAFT_2540 at 730.727 kb on - strand, within B158DRAFT_2540 at 730.898 kb on + strand, within B158DRAFT_2540 at 730.914 kb on - strand, within B158DRAFT_2540 at 730.939 kb on + strand, within B158DRAFT_2540 at 731.064 kb on - strand, within B158DRAFT_2540 at 731.074 kb on - strand, within B158DRAFT_2540 at 731.089 kb on - strand at 731.236 kb on + strand, within B158DRAFT_2541 at 731.255 kb on + strand, within B158DRAFT_2541 at 731.263 kb on - strand, within B158DRAFT_2541 at 731.273 kb on - strand, within B158DRAFT_2541 at 731.273 kb on - strand, within B158DRAFT_2541 at 731.299 kb on + strand, within B158DRAFT_2541 at 731.299 kb on + strand, within B158DRAFT_2541 at 731.299 kb on - strand, within B158DRAFT_2541 at 731.307 kb on - strand, within B158DRAFT_2541 at 731.336 kb on + strand, within B158DRAFT_2541 at 731.336 kb on + strand, within B158DRAFT_2541 at 731.336 kb on + strand, within B158DRAFT_2541 at 731.339 kb on - strand, within B158DRAFT_2541 at 731.402 kb on + strand, within B158DRAFT_2541 at 731.402 kb on + strand, within B158DRAFT_2541 at 731.405 kb on - strand, within B158DRAFT_2541 at 731.410 kb on - strand, within B158DRAFT_2541 at 731.411 kb on - strand, within B158DRAFT_2541 at 731.455 kb on + strand at 731.463 kb on - strand at 731.493 kb on + strand at 731.527 kb on + strand at 731.573 kb on - strand at 731.575 kb on + strand at 731.575 kb on + strand at 731.583 kb on - strand at 731.596 kb on + strand at 731.596 kb on + strand at 731.596 kb on + strand at 731.606 kb on + strand at 731.606 kb on + strand at 732.362 kb on + strand, within B158DRAFT_2542 at 732.399 kb on + strand at 732.471 kb on - strand at 732.471 kb on - strand at 732.471 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Gentamicin 0.1 mg/ml remove 730,308 + B158DRAFT_2539 0.49 -1.4 730,311 - B158DRAFT_2539 0.50 -0.4 730,311 - B158DRAFT_2539 0.50 +0.3 730,343 + B158DRAFT_2539 0.55 +0.6 730,357 + B158DRAFT_2539 0.58 +0.5 730,357 + B158DRAFT_2539 0.58 +0.3 730,357 + B158DRAFT_2539 0.58 +0.1 730,365 - B158DRAFT_2539 0.59 -0.5 730,365 - B158DRAFT_2539 0.59 +0.7 730,367 + B158DRAFT_2539 0.60 -1.8 730,377 - B158DRAFT_2539 0.61 -3.6 730,377 - B158DRAFT_2539 0.61 -3.8 730,398 + B158DRAFT_2539 0.65 +0.3 730,442 - B158DRAFT_2539 0.73 +1.4 730,442 - B158DRAFT_2539 0.73 +0.5 730,515 + B158DRAFT_2539 0.86 -3.0 730,541 + -1.9 730,555 + +0.3 730,719 + B158DRAFT_2540 0.13 -0.9 730,719 + B158DRAFT_2540 0.13 +0.4 730,727 - B158DRAFT_2540 0.15 +0.0 730,898 + B158DRAFT_2540 0.51 -2.1 730,914 - B158DRAFT_2540 0.54 -0.4 730,939 + B158DRAFT_2540 0.59 -0.3 731,064 - B158DRAFT_2540 0.85 -0.8 731,074 - B158DRAFT_2540 0.87 -1.4 731,089 - -0.7 731,236 + B158DRAFT_2541 0.16 -4.5 731,255 + B158DRAFT_2541 0.23 -1.1 731,263 - B158DRAFT_2541 0.26 +0.2 731,273 - B158DRAFT_2541 0.29 -0.2 731,273 - B158DRAFT_2541 0.29 +0.7 731,299 + B158DRAFT_2541 0.38 -0.1 731,299 + B158DRAFT_2541 0.38 +0.2 731,299 - B158DRAFT_2541 0.38 -0.9 731,307 - B158DRAFT_2541 0.41 +0.4 731,336 + B158DRAFT_2541 0.51 -0.1 731,336 + B158DRAFT_2541 0.51 -0.7 731,336 + B158DRAFT_2541 0.51 +0.1 731,339 - B158DRAFT_2541 0.52 -0.3 731,402 + B158DRAFT_2541 0.75 +0.0 731,402 + B158DRAFT_2541 0.75 +0.7 731,405 - B158DRAFT_2541 0.76 -1.0 731,410 - B158DRAFT_2541 0.78 +1.0 731,411 - B158DRAFT_2541 0.78 +0.8 731,455 + +0.5 731,463 - +0.9 731,493 + +1.4 731,527 + +0.8 731,573 - -3.0 731,575 + -0.3 731,575 + +0.4 731,583 - -1.3 731,596 + +0.2 731,596 + -1.5 731,596 + -0.4 731,606 + -1.1 731,606 + -4.3 732,362 + B158DRAFT_2542 0.88 +0.5 732,399 + +0.3 732,471 - +0.3 732,471 - -0.5 732,471 - -0.7
Or see this region's nucleotide sequence