Experiment: m.b. Gentamicin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2289 and B158DRAFT_2290 overlap by 4 nucleotides B158DRAFT_2290 and B158DRAFT_2291 overlap by 13 nucleotides B158DRAFT_2291 and B158DRAFT_2292 are separated by 12 nucleotides
B158DRAFT_2289: B158DRAFT_2289 - imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19), at 454,537 to 455,133
_2289
B158DRAFT_2290: B158DRAFT_2290 - imidazole glycerol phosphate synthase, glutamine amidotransferase subunit, at 455,130 to 455,738
_2290
B158DRAFT_2291: B158DRAFT_2291 - imidazoleglycerol phosphate synthase, cyclase subunit, at 455,726 to 456,529
_2291
B158DRAFT_2292: B158DRAFT_2292 - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (EC 5.3.1.16)/phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19)/phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31), at 456,542 to 457,780
_2292
Position (kb)
455
456
457 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 454.797 kb on + strand, within B158DRAFT_2289 at 454.797 kb on + strand, within B158DRAFT_2289 at 454.805 kb on - strand, within B158DRAFT_2289 at 454.858 kb on + strand, within B158DRAFT_2289 at 454.863 kb on + strand, within B158DRAFT_2289 at 454.866 kb on - strand, within B158DRAFT_2289 at 454.874 kb on + strand, within B158DRAFT_2289 at 454.876 kb on + strand, within B158DRAFT_2289 at 454.968 kb on + strand, within B158DRAFT_2289 at 454.969 kb on + strand, within B158DRAFT_2289 at 455.007 kb on + strand, within B158DRAFT_2289 at 455.007 kb on + strand, within B158DRAFT_2289 at 455.007 kb on + strand, within B158DRAFT_2289 at 455.007 kb on + strand, within B158DRAFT_2289 at 455.015 kb on - strand, within B158DRAFT_2289 at 455.015 kb on - strand, within B158DRAFT_2289 at 455.015 kb on - strand, within B158DRAFT_2289 at 455.015 kb on - strand, within B158DRAFT_2289 at 455.044 kb on + strand, within B158DRAFT_2289 at 455.069 kb on + strand, within B158DRAFT_2289 at 455.077 kb on - strand at 455.093 kb on + strand at 455.122 kb on - strand at 455.129 kb on - strand at 455.137 kb on + strand at 455.137 kb on + strand at 455.224 kb on + strand, within B158DRAFT_2290 at 455.230 kb on + strand, within B158DRAFT_2290 at 455.246 kb on - strand, within B158DRAFT_2290 at 455.276 kb on + strand, within B158DRAFT_2290 at 455.279 kb on - strand, within B158DRAFT_2290 at 455.349 kb on - strand, within B158DRAFT_2290 at 455.391 kb on + strand, within B158DRAFT_2290 at 455.398 kb on + strand, within B158DRAFT_2290 at 455.409 kb on + strand, within B158DRAFT_2290 at 455.412 kb on - strand, within B158DRAFT_2290 at 455.412 kb on - strand, within B158DRAFT_2290 at 455.420 kb on - strand, within B158DRAFT_2290 at 455.591 kb on + strand, within B158DRAFT_2290 at 455.606 kb on + strand, within B158DRAFT_2290 at 455.614 kb on - strand, within B158DRAFT_2290 at 455.727 kb on + strand at 455.792 kb on + strand at 455.795 kb on - strand at 455.799 kb on + strand at 455.828 kb on + strand, within B158DRAFT_2291 at 455.836 kb on + strand, within B158DRAFT_2291 at 455.836 kb on + strand, within B158DRAFT_2291 at 455.836 kb on - strand, within B158DRAFT_2291 at 455.836 kb on - strand, within B158DRAFT_2291 at 455.844 kb on - strand, within B158DRAFT_2291 at 455.844 kb on - strand, within B158DRAFT_2291 at 455.844 kb on - strand, within B158DRAFT_2291 at 455.845 kb on - strand, within B158DRAFT_2291 at 455.927 kb on - strand, within B158DRAFT_2291 at 455.949 kb on + strand, within B158DRAFT_2291 at 455.957 kb on - strand, within B158DRAFT_2291 at 456.064 kb on - strand, within B158DRAFT_2291 at 456.126 kb on + strand, within B158DRAFT_2291 at 456.257 kb on + strand, within B158DRAFT_2291 at 456.257 kb on + strand, within B158DRAFT_2291 at 456.263 kb on + strand, within B158DRAFT_2291 at 456.314 kb on + strand, within B158DRAFT_2291 at 456.322 kb on + strand, within B158DRAFT_2291 at 456.493 kb on + strand at 456.493 kb on + strand at 456.503 kb on - strand at 456.503 kb on - strand at 456.555 kb on + strand at 456.571 kb on + strand at 456.595 kb on + strand at 456.603 kb on - strand at 456.700 kb on + strand, within B158DRAFT_2292 at 456.703 kb on - strand, within B158DRAFT_2292 at 456.708 kb on - strand, within B158DRAFT_2292 at 456.708 kb on - strand, within B158DRAFT_2292 at 456.709 kb on - strand, within B158DRAFT_2292 at 456.796 kb on - strand, within B158DRAFT_2292 at 456.810 kb on - strand, within B158DRAFT_2292 at 456.926 kb on - strand, within B158DRAFT_2292 at 456.939 kb on - strand, within B158DRAFT_2292 at 456.995 kb on + strand, within B158DRAFT_2292 at 456.997 kb on - strand, within B158DRAFT_2292 at 457.055 kb on - strand, within B158DRAFT_2292 at 457.055 kb on - strand, within B158DRAFT_2292 at 457.106 kb on + strand, within B158DRAFT_2292 at 457.114 kb on - strand, within B158DRAFT_2292 at 457.159 kb on - strand, within B158DRAFT_2292 at 457.207 kb on - strand, within B158DRAFT_2292 at 457.207 kb on - strand, within B158DRAFT_2292 at 457.207 kb on - strand, within B158DRAFT_2292 at 457.207 kb on - strand, within B158DRAFT_2292 at 457.207 kb on - strand, within B158DRAFT_2292 at 457.255 kb on - strand, within B158DRAFT_2292 at 457.376 kb on - strand, within B158DRAFT_2292 at 457.410 kb on - strand, within B158DRAFT_2292 at 457.467 kb on - strand, within B158DRAFT_2292
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Gentamicin 0.1 mg/ml remove 454,797 + B158DRAFT_2289 0.44 -1.4 454,797 + B158DRAFT_2289 0.44 +0.5 454,805 - B158DRAFT_2289 0.45 -0.3 454,858 + B158DRAFT_2289 0.54 +0.0 454,863 + B158DRAFT_2289 0.55 -0.7 454,866 - B158DRAFT_2289 0.55 -0.2 454,874 + B158DRAFT_2289 0.56 -0.4 454,876 + B158DRAFT_2289 0.57 -0.1 454,968 + B158DRAFT_2289 0.72 -2.8 454,969 + B158DRAFT_2289 0.72 +0.2 455,007 + B158DRAFT_2289 0.79 -4.0 455,007 + B158DRAFT_2289 0.79 +0.6 455,007 + B158DRAFT_2289 0.79 -0.0 455,007 + B158DRAFT_2289 0.79 -0.1 455,015 - B158DRAFT_2289 0.80 -0.1 455,015 - B158DRAFT_2289 0.80 +0.4 455,015 - B158DRAFT_2289 0.80 -0.4 455,015 - B158DRAFT_2289 0.80 +0.3 455,044 + B158DRAFT_2289 0.85 -0.3 455,069 + B158DRAFT_2289 0.89 -0.4 455,077 - +0.6 455,093 + +0.3 455,122 - +0.2 455,129 - +0.9 455,137 + -0.5 455,137 + -0.3 455,224 + B158DRAFT_2290 0.15 -2.5 455,230 + B158DRAFT_2290 0.16 +0.3 455,246 - B158DRAFT_2290 0.19 -0.1 455,276 + B158DRAFT_2290 0.24 -0.1 455,279 - B158DRAFT_2290 0.24 -0.4 455,349 - B158DRAFT_2290 0.36 +0.7 455,391 + B158DRAFT_2290 0.43 -1.5 455,398 + B158DRAFT_2290 0.44 -0.8 455,409 + B158DRAFT_2290 0.46 -3.9 455,412 - B158DRAFT_2290 0.46 -0.2 455,412 - B158DRAFT_2290 0.46 -1.0 455,420 - B158DRAFT_2290 0.48 -2.2 455,591 + B158DRAFT_2290 0.76 -0.2 455,606 + B158DRAFT_2290 0.78 -0.4 455,614 - B158DRAFT_2290 0.79 -4.5 455,727 + -4.0 455,792 + -0.9 455,795 - +0.2 455,799 + +1.2 455,828 + B158DRAFT_2291 0.13 -0.1 455,836 + B158DRAFT_2291 0.14 +0.8 455,836 + B158DRAFT_2291 0.14 -0.8 455,836 - B158DRAFT_2291 0.14 +1.0 455,836 - B158DRAFT_2291 0.14 +0.3 455,844 - B158DRAFT_2291 0.15 -1.8 455,844 - B158DRAFT_2291 0.15 +2.1 455,844 - B158DRAFT_2291 0.15 -2.5 455,845 - B158DRAFT_2291 0.15 -0.7 455,927 - B158DRAFT_2291 0.25 +0.2 455,949 + B158DRAFT_2291 0.28 +0.3 455,957 - B158DRAFT_2291 0.29 +0.5 456,064 - B158DRAFT_2291 0.42 -0.5 456,126 + B158DRAFT_2291 0.50 +1.0 456,257 + B158DRAFT_2291 0.66 +0.4 456,257 + B158DRAFT_2291 0.66 +1.0 456,263 + B158DRAFT_2291 0.67 -3.1 456,314 + B158DRAFT_2291 0.73 -0.8 456,322 + B158DRAFT_2291 0.74 -1.1 456,493 + +1.6 456,493 + -1.8 456,503 - -0.4 456,503 - -3.8 456,555 + -0.1 456,571 + +0.0 456,595 + -2.8 456,603 - -1.2 456,700 + B158DRAFT_2292 0.13 +0.7 456,703 - B158DRAFT_2292 0.13 -0.0 456,708 - B158DRAFT_2292 0.13 -0.3 456,708 - B158DRAFT_2292 0.13 -0.2 456,709 - B158DRAFT_2292 0.13 -1.6 456,796 - B158DRAFT_2292 0.21 -0.2 456,810 - B158DRAFT_2292 0.22 -4.9 456,926 - B158DRAFT_2292 0.31 +0.2 456,939 - B158DRAFT_2292 0.32 +0.0 456,995 + B158DRAFT_2292 0.37 -0.3 456,997 - B158DRAFT_2292 0.37 +0.1 457,055 - B158DRAFT_2292 0.41 -0.5 457,055 - B158DRAFT_2292 0.41 -3.7 457,106 + B158DRAFT_2292 0.46 -0.0 457,114 - B158DRAFT_2292 0.46 +0.5 457,159 - B158DRAFT_2292 0.50 -0.1 457,207 - B158DRAFT_2292 0.54 -4.2 457,207 - B158DRAFT_2292 0.54 -1.0 457,207 - B158DRAFT_2292 0.54 +0.5 457,207 - B158DRAFT_2292 0.54 -0.4 457,207 - B158DRAFT_2292 0.54 -0.9 457,255 - B158DRAFT_2292 0.58 -1.2 457,376 - B158DRAFT_2292 0.67 +0.3 457,410 - B158DRAFT_2292 0.70 -1.1 457,467 - B158DRAFT_2292 0.75 +1.0
Or see this region's nucleotide sequence