Experiment: m.b. Gentamicin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_0639 and B158DRAFT_0640 are separated by 12 nucleotides B158DRAFT_0640 and B158DRAFT_0641 are separated by 229 nucleotides B158DRAFT_0641 and B158DRAFT_0642 are separated by 37 nucleotides B158DRAFT_0642 and B158DRAFT_0643 are separated by 46 nucleotides
B158DRAFT_0639: B158DRAFT_0639 - Predicted transcriptional regulator, contains C-terminal CBS domains, at 648,389 to 648,793
_0639
B158DRAFT_0640: B158DRAFT_0640 - Uncharacterized protein conserved in bacteria, at 648,806 to 649,024
_0640
B158DRAFT_0641: B158DRAFT_0641 - hypothetical protein, at 649,254 to 649,631
_0641
B158DRAFT_0642: B158DRAFT_0642 - Membrane-associated lipoprotein involved in thiamine biosynthesis, at 649,669 to 650,700
_0642
B158DRAFT_0643: B158DRAFT_0643 - NADH:ubiquinone oxidoreductase, Na(+)-translocating, F subunit, at 650,747 to 651,973
_0643
Position (kb) 649
650
651 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 649.138 kb on - strand at 649.151 kb on + strand at 649.159 kb on - strand at 649.203 kb on + strand at 649.311 kb on + strand, within B158DRAFT_0641 at 649.313 kb on + strand, within B158DRAFT_0641 at 649.378 kb on + strand, within B158DRAFT_0641 at 649.383 kb on + strand, within B158DRAFT_0641 at 649.386 kb on - strand, within B158DRAFT_0641 at 649.549 kb on + strand, within B158DRAFT_0641 at 649.566 kb on + strand, within B158DRAFT_0641 at 649.566 kb on + strand, within B158DRAFT_0641 at 649.574 kb on - strand, within B158DRAFT_0641 at 649.626 kb on + strand at 650.736 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Gentamicin 0.1 mg/ml remove 649,138 - +0.1 649,151 + +0.1 649,159 - +0.2 649,203 + +0.0 649,311 + B158DRAFT_0641 0.15 -2.5 649,313 + B158DRAFT_0641 0.16 -0.8 649,378 + B158DRAFT_0641 0.33 +0.0 649,383 + B158DRAFT_0641 0.34 -0.8 649,386 - B158DRAFT_0641 0.35 +0.3 649,549 + B158DRAFT_0641 0.78 +0.5 649,566 + B158DRAFT_0641 0.83 -0.8 649,566 + B158DRAFT_0641 0.83 +0.4 649,574 - B158DRAFT_0641 0.85 +0.1 649,626 + -4.6 650,736 + +0.6
Or see this region's nucleotide sequence