Experiment: m.b. Gentamicin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_0062 and B158DRAFT_0063 are separated by 143 nucleotides B158DRAFT_0063 and B158DRAFT_0064 are separated by 64 nucleotides
B158DRAFT_0062: B158DRAFT_0062 - pantoate--beta-alanine ligase, at 58,891 to 59,736
_0062
B158DRAFT_0063: B158DRAFT_0063 - acetate--CoA ligase, at 59,880 to 61,814
_0063
B158DRAFT_0064: B158DRAFT_0064 - Uncharacterized protein conserved in bacteria, at 61,879 to 62,751
_0064
Position (kb)
59
60
61
62 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 58.881 kb on + strand at 59.725 kb on - strand at 59.738 kb on - strand at 59.853 kb on + strand at 59.912 kb on + strand at 59.990 kb on + strand at 59.998 kb on - strand at 60.025 kb on + strand at 60.025 kb on + strand at 60.025 kb on + strand at 60.033 kb on - strand at 60.033 kb on - strand at 60.033 kb on - strand at 60.033 kb on - strand at 60.033 kb on - strand at 60.118 kb on - strand, within B158DRAFT_0063 at 60.210 kb on - strand, within B158DRAFT_0063 at 60.213 kb on - strand, within B158DRAFT_0063 at 60.251 kb on - strand, within B158DRAFT_0063 at 60.343 kb on + strand, within B158DRAFT_0063 at 60.353 kb on - strand, within B158DRAFT_0063 at 60.360 kb on - strand, within B158DRAFT_0063 at 60.402 kb on - strand, within B158DRAFT_0063 at 60.492 kb on + strand, within B158DRAFT_0063 at 60.492 kb on + strand, within B158DRAFT_0063 at 60.492 kb on + strand, within B158DRAFT_0063 at 60.492 kb on + strand, within B158DRAFT_0063 at 60.492 kb on + strand, within B158DRAFT_0063 at 60.492 kb on + strand, within B158DRAFT_0063 at 60.492 kb on + strand, within B158DRAFT_0063 at 60.492 kb on + strand, within B158DRAFT_0063 at 60.492 kb on + strand, within B158DRAFT_0063 at 60.500 kb on - strand, within B158DRAFT_0063 at 60.500 kb on - strand, within B158DRAFT_0063 at 60.500 kb on - strand, within B158DRAFT_0063 at 60.500 kb on - strand, within B158DRAFT_0063 at 60.500 kb on - strand, within B158DRAFT_0063 at 60.502 kb on + strand, within B158DRAFT_0063 at 60.502 kb on + strand, within B158DRAFT_0063 at 60.502 kb on + strand, within B158DRAFT_0063 at 60.502 kb on + strand, within B158DRAFT_0063 at 60.502 kb on + strand, within B158DRAFT_0063 at 60.502 kb on + strand, within B158DRAFT_0063 at 60.502 kb on + strand, within B158DRAFT_0063 at 60.502 kb on + strand, within B158DRAFT_0063 at 60.502 kb on + strand, within B158DRAFT_0063 at 60.507 kb on + strand, within B158DRAFT_0063 at 60.510 kb on - strand, within B158DRAFT_0063 at 60.510 kb on - strand, within B158DRAFT_0063 at 60.510 kb on - strand, within B158DRAFT_0063 at 60.510 kb on - strand, within B158DRAFT_0063 at 60.510 kb on - strand, within B158DRAFT_0063 at 60.510 kb on - strand, within B158DRAFT_0063 at 60.510 kb on - strand, within B158DRAFT_0063 at 60.510 kb on - strand, within B158DRAFT_0063 at 60.510 kb on - strand, within B158DRAFT_0063 at 60.510 kb on - strand, within B158DRAFT_0063 at 60.510 kb on - strand, within B158DRAFT_0063 at 60.510 kb on - strand, within B158DRAFT_0063 at 60.510 kb on - strand, within B158DRAFT_0063 at 60.510 kb on - strand, within B158DRAFT_0063 at 60.510 kb on - strand, within B158DRAFT_0063 at 60.515 kb on - strand, within B158DRAFT_0063 at 60.604 kb on + strand, within B158DRAFT_0063 at 60.664 kb on - strand, within B158DRAFT_0063 at 60.664 kb on - strand, within B158DRAFT_0063 at 60.669 kb on - strand, within B158DRAFT_0063 at 60.669 kb on - strand, within B158DRAFT_0063 at 60.690 kb on + strand, within B158DRAFT_0063 at 60.690 kb on + strand, within B158DRAFT_0063 at 60.690 kb on + strand, within B158DRAFT_0063 at 60.690 kb on + strand, within B158DRAFT_0063 at 60.698 kb on - strand, within B158DRAFT_0063 at 60.698 kb on - strand, within B158DRAFT_0063 at 60.726 kb on + strand, within B158DRAFT_0063 at 60.734 kb on - strand, within B158DRAFT_0063 at 60.776 kb on + strand, within B158DRAFT_0063 at 60.791 kb on - strand, within B158DRAFT_0063 at 60.828 kb on + strand, within B158DRAFT_0063 at 60.857 kb on + strand, within B158DRAFT_0063 at 60.914 kb on + strand, within B158DRAFT_0063 at 60.922 kb on - strand, within B158DRAFT_0063 at 60.928 kb on - strand, within B158DRAFT_0063 at 61.019 kb on + strand, within B158DRAFT_0063 at 61.090 kb on + strand, within B158DRAFT_0063 at 61.098 kb on - strand, within B158DRAFT_0063 at 61.148 kb on + strand, within B158DRAFT_0063 at 61.148 kb on + strand, within B158DRAFT_0063 at 61.156 kb on - strand, within B158DRAFT_0063 at 61.161 kb on - strand, within B158DRAFT_0063 at 61.174 kb on + strand, within B158DRAFT_0063 at 61.255 kb on + strand, within B158DRAFT_0063 at 61.263 kb on - strand, within B158DRAFT_0063 at 61.284 kb on + strand, within B158DRAFT_0063 at 61.284 kb on + strand, within B158DRAFT_0063 at 61.284 kb on + strand, within B158DRAFT_0063 at 61.284 kb on + strand, within B158DRAFT_0063 at 61.284 kb on + strand, within B158DRAFT_0063 at 61.284 kb on + strand, within B158DRAFT_0063 at 61.284 kb on + strand, within B158DRAFT_0063 at 61.292 kb on - strand, within B158DRAFT_0063 at 61.292 kb on - strand, within B158DRAFT_0063 at 61.292 kb on - strand, within B158DRAFT_0063 at 61.292 kb on - strand, within B158DRAFT_0063 at 61.292 kb on - strand, within B158DRAFT_0063 at 61.292 kb on - strand, within B158DRAFT_0063 at 61.292 kb on - strand, within B158DRAFT_0063 at 61.292 kb on - strand, within B158DRAFT_0063 at 61.292 kb on - strand, within B158DRAFT_0063 at 61.307 kb on - strand, within B158DRAFT_0063 at 61.358 kb on - strand, within B158DRAFT_0063 at 61.397 kb on + strand, within B158DRAFT_0063 at 61.445 kb on + strand, within B158DRAFT_0063 at 61.448 kb on + strand, within B158DRAFT_0063 at 61.453 kb on - strand, within B158DRAFT_0063 at 61.479 kb on + strand, within B158DRAFT_0063 at 61.482 kb on - strand, within B158DRAFT_0063 at 61.487 kb on - strand, within B158DRAFT_0063 at 61.618 kb on + strand, within B158DRAFT_0063 at 61.671 kb on + strand at 61.676 kb on + strand at 61.684 kb on - strand at 61.856 kb on + strand at 61.857 kb on - strand at 61.878 kb on - strand at 61.879 kb on - strand at 61.882 kb on + strand at 61.885 kb on - strand at 61.921 kb on + strand at 62.105 kb on + strand, within B158DRAFT_0064 at 62.113 kb on - strand, within B158DRAFT_0064 at 62.113 kb on - strand, within B158DRAFT_0064 at 62.126 kb on + strand, within B158DRAFT_0064 at 62.134 kb on - strand, within B158DRAFT_0064 at 62.165 kb on - strand, within B158DRAFT_0064 at 62.232 kb on - strand, within B158DRAFT_0064 at 62.259 kb on + strand, within B158DRAFT_0064 at 62.285 kb on + strand, within B158DRAFT_0064 at 62.301 kb on + strand, within B158DRAFT_0064 at 62.349 kb on + strand, within B158DRAFT_0064 at 62.349 kb on + strand, within B158DRAFT_0064 at 62.349 kb on + strand, within B158DRAFT_0064 at 62.349 kb on + strand, within B158DRAFT_0064 at 62.357 kb on - strand, within B158DRAFT_0064 at 62.357 kb on - strand, within B158DRAFT_0064 at 62.357 kb on - strand, within B158DRAFT_0064 at 62.357 kb on - strand, within B158DRAFT_0064 at 62.357 kb on - strand, within B158DRAFT_0064 at 62.357 kb on - strand, within B158DRAFT_0064 at 62.357 kb on - strand, within B158DRAFT_0064 at 62.451 kb on + strand, within B158DRAFT_0064 at 62.510 kb on - strand, within B158DRAFT_0064 at 62.547 kb on - strand, within B158DRAFT_0064 at 62.549 kb on + strand, within B158DRAFT_0064 at 62.557 kb on - strand, within B158DRAFT_0064 at 62.559 kb on + strand, within B158DRAFT_0064 at 62.579 kb on - strand, within B158DRAFT_0064 at 62.642 kb on + strand, within B158DRAFT_0064 at 62.650 kb on - strand, within B158DRAFT_0064 at 62.650 kb on - strand, within B158DRAFT_0064 at 62.702 kb on - strand at 62.753 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Gentamicin 0.1 mg/ml remove 58,881 + +2.0 59,725 - +0.9 59,738 - +0.1 59,853 + +0.8 59,912 + -0.2 59,990 + -0.4 59,998 - +0.8 60,025 + -2.9 60,025 + -0.3 60,025 + -0.5 60,033 - +1.1 60,033 - -0.8 60,033 - +0.5 60,033 - -0.7 60,033 - -0.2 60,118 - B158DRAFT_0063 0.12 -0.1 60,210 - B158DRAFT_0063 0.17 -0.4 60,213 - B158DRAFT_0063 0.17 +0.7 60,251 - B158DRAFT_0063 0.19 -0.0 60,343 + B158DRAFT_0063 0.24 -0.8 60,353 - B158DRAFT_0063 0.24 -0.5 60,360 - B158DRAFT_0063 0.25 -0.7 60,402 - B158DRAFT_0063 0.27 +0.7 60,492 + B158DRAFT_0063 0.32 +0.0 60,492 + B158DRAFT_0063 0.32 -0.1 60,492 + B158DRAFT_0063 0.32 +2.1 60,492 + B158DRAFT_0063 0.32 -0.5 60,492 + B158DRAFT_0063 0.32 +0.1 60,492 + B158DRAFT_0063 0.32 -0.1 60,492 + B158DRAFT_0063 0.32 -0.7 60,492 + B158DRAFT_0063 0.32 +0.6 60,492 + B158DRAFT_0063 0.32 +1.8 60,500 - B158DRAFT_0063 0.32 -1.6 60,500 - B158DRAFT_0063 0.32 +0.3 60,500 - B158DRAFT_0063 0.32 +0.4 60,500 - B158DRAFT_0063 0.32 -0.8 60,500 - B158DRAFT_0063 0.32 -0.1 60,502 + B158DRAFT_0063 0.32 +0.8 60,502 + B158DRAFT_0063 0.32 +1.2 60,502 + B158DRAFT_0063 0.32 -3.3 60,502 + B158DRAFT_0063 0.32 -1.1 60,502 + B158DRAFT_0063 0.32 -1.1 60,502 + B158DRAFT_0063 0.32 -1.2 60,502 + B158DRAFT_0063 0.32 +0.6 60,502 + B158DRAFT_0063 0.32 +0.8 60,502 + B158DRAFT_0063 0.32 -0.3 60,507 + B158DRAFT_0063 0.32 +0.8 60,510 - B158DRAFT_0063 0.33 -4.4 60,510 - B158DRAFT_0063 0.33 -0.7 60,510 - B158DRAFT_0063 0.33 +0.2 60,510 - B158DRAFT_0063 0.33 -0.3 60,510 - B158DRAFT_0063 0.33 +0.8 60,510 - B158DRAFT_0063 0.33 -0.2 60,510 - B158DRAFT_0063 0.33 -0.8 60,510 - B158DRAFT_0063 0.33 +1.0 60,510 - B158DRAFT_0063 0.33 -0.3 60,510 - B158DRAFT_0063 0.33 -3.1 60,510 - B158DRAFT_0063 0.33 -0.1 60,510 - B158DRAFT_0063 0.33 +0.4 60,510 - B158DRAFT_0063 0.33 +1.8 60,510 - B158DRAFT_0063 0.33 +0.9 60,510 - B158DRAFT_0063 0.33 -1.1 60,515 - B158DRAFT_0063 0.33 +0.1 60,604 + B158DRAFT_0063 0.37 +0.3 60,664 - B158DRAFT_0063 0.41 -0.4 60,664 - B158DRAFT_0063 0.41 -0.5 60,669 - B158DRAFT_0063 0.41 +0.3 60,669 - B158DRAFT_0063 0.41 -2.0 60,690 + B158DRAFT_0063 0.42 -0.5 60,690 + B158DRAFT_0063 0.42 -1.5 60,690 + B158DRAFT_0063 0.42 +0.6 60,690 + B158DRAFT_0063 0.42 -1.1 60,698 - B158DRAFT_0063 0.42 +0.3 60,698 - B158DRAFT_0063 0.42 +0.5 60,726 + B158DRAFT_0063 0.44 -1.5 60,734 - B158DRAFT_0063 0.44 -0.2 60,776 + B158DRAFT_0063 0.46 -0.6 60,791 - B158DRAFT_0063 0.47 -0.5 60,828 + B158DRAFT_0063 0.49 -0.4 60,857 + B158DRAFT_0063 0.50 -0.5 60,914 + B158DRAFT_0063 0.53 -2.1 60,922 - B158DRAFT_0063 0.54 -0.0 60,928 - B158DRAFT_0063 0.54 +0.0 61,019 + B158DRAFT_0063 0.59 +1.3 61,090 + B158DRAFT_0063 0.63 +0.4 61,098 - B158DRAFT_0063 0.63 -0.2 61,148 + B158DRAFT_0063 0.66 -0.3 61,148 + B158DRAFT_0063 0.66 -0.9 61,156 - B158DRAFT_0063 0.66 -0.5 61,161 - B158DRAFT_0063 0.66 +1.1 61,174 + B158DRAFT_0063 0.67 +0.1 61,255 + B158DRAFT_0063 0.71 -0.9 61,263 - B158DRAFT_0063 0.71 +0.0 61,284 + B158DRAFT_0063 0.73 -0.2 61,284 + B158DRAFT_0063 0.73 +0.1 61,284 + B158DRAFT_0063 0.73 +0.6 61,284 + B158DRAFT_0063 0.73 +0.4 61,284 + B158DRAFT_0063 0.73 +0.3 61,284 + B158DRAFT_0063 0.73 +1.3 61,284 + B158DRAFT_0063 0.73 -0.9 61,292 - B158DRAFT_0063 0.73 -1.0 61,292 - B158DRAFT_0063 0.73 -0.6 61,292 - B158DRAFT_0063 0.73 +1.6 61,292 - B158DRAFT_0063 0.73 -0.1 61,292 - B158DRAFT_0063 0.73 -0.4 61,292 - B158DRAFT_0063 0.73 +0.9 61,292 - B158DRAFT_0063 0.73 -0.3 61,292 - B158DRAFT_0063 0.73 -0.4 61,292 - B158DRAFT_0063 0.73 -2.2 61,307 - B158DRAFT_0063 0.74 -0.5 61,358 - B158DRAFT_0063 0.76 -0.4 61,397 + B158DRAFT_0063 0.78 -0.5 61,445 + B158DRAFT_0063 0.81 -0.5 61,448 + B158DRAFT_0063 0.81 -0.2 61,453 - B158DRAFT_0063 0.81 -0.1 61,479 + B158DRAFT_0063 0.83 -0.3 61,482 - B158DRAFT_0063 0.83 -1.4 61,487 - B158DRAFT_0063 0.83 +0.5 61,618 + B158DRAFT_0063 0.90 +0.7 61,671 + +0.1 61,676 + +0.5 61,684 - -0.1 61,856 + +0.5 61,857 - +0.3 61,878 - -0.0 61,879 - +0.5 61,882 + +0.1 61,885 - -0.3 61,921 + -0.1 62,105 + B158DRAFT_0064 0.26 +0.1 62,113 - B158DRAFT_0064 0.27 +0.2 62,113 - B158DRAFT_0064 0.27 -0.2 62,126 + B158DRAFT_0064 0.28 +0.4 62,134 - B158DRAFT_0064 0.29 +1.3 62,165 - B158DRAFT_0064 0.33 -1.4 62,232 - B158DRAFT_0064 0.40 -0.4 62,259 + B158DRAFT_0064 0.44 -0.0 62,285 + B158DRAFT_0064 0.47 +0.1 62,301 + B158DRAFT_0064 0.48 +0.3 62,349 + B158DRAFT_0064 0.54 +0.4 62,349 + B158DRAFT_0064 0.54 -0.1 62,349 + B158DRAFT_0064 0.54 -0.3 62,349 + B158DRAFT_0064 0.54 -0.7 62,357 - B158DRAFT_0064 0.55 -0.4 62,357 - B158DRAFT_0064 0.55 +0.0 62,357 - B158DRAFT_0064 0.55 +0.8 62,357 - B158DRAFT_0064 0.55 -1.2 62,357 - B158DRAFT_0064 0.55 +0.4 62,357 - B158DRAFT_0064 0.55 -0.7 62,357 - B158DRAFT_0064 0.55 -0.5 62,451 + B158DRAFT_0064 0.66 +0.0 62,510 - B158DRAFT_0064 0.72 +0.7 62,547 - B158DRAFT_0064 0.77 +0.8 62,549 + B158DRAFT_0064 0.77 -1.3 62,557 - B158DRAFT_0064 0.78 +0.9 62,559 + B158DRAFT_0064 0.78 -0.1 62,579 - B158DRAFT_0064 0.80 +0.9 62,642 + B158DRAFT_0064 0.87 -0.5 62,650 - B158DRAFT_0064 0.88 +0.8 62,650 - B158DRAFT_0064 0.88 -0.7 62,702 - +0.9 62,753 + -0.4
Or see this region's nucleotide sequence