Experiment: m.b. Gentamicin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_0029 and B158DRAFT_0030 are separated by 309 nucleotides B158DRAFT_0030 and B158DRAFT_0031 are separated by 11 nucleotides B158DRAFT_0031 and B158DRAFT_0032 are separated by 3 nucleotides
B158DRAFT_0029: B158DRAFT_0029 - Uncharacterized protein conserved in bacteria, at 29,019 to 29,504
_0029
B158DRAFT_0030: B158DRAFT_0030 - Putative translation initiation inhibitor, yjgF family, at 29,814 to 30,236
_0030
B158DRAFT_0031: B158DRAFT_0031 - 3-hydroxyanthranilate 3,4-dioxygenase (EC 1.13.11.6), at 30,248 to 30,790
_0031
B158DRAFT_0032: B158DRAFT_0032 - Predicted metal-dependent hydrolase of the TIM-barrel fold, at 30,794 to 31,810
_0032
Position (kb)
29
30
31 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 28.860 kb on - strand at 28.866 kb on - strand at 28.882 kb on + strand at 28.883 kb on + strand at 28.891 kb on - strand at 28.891 kb on - strand at 28.891 kb on - strand at 28.893 kb on + strand at 28.905 kb on + strand at 28.945 kb on + strand at 28.945 kb on + strand at 28.952 kb on + strand at 28.952 kb on + strand at 28.952 kb on + strand at 28.952 kb on + strand at 28.952 kb on + strand at 28.952 kb on + strand at 28.953 kb on - strand at 28.960 kb on - strand at 28.960 kb on - strand at 28.960 kb on - strand at 28.960 kb on - strand at 29.010 kb on + strand at 29.010 kb on + strand at 29.010 kb on + strand at 29.015 kb on + strand at 29.018 kb on - strand at 29.018 kb on - strand at 29.018 kb on - strand at 29.018 kb on - strand at 29.018 kb on - strand at 29.094 kb on + strand, within B158DRAFT_0029 at 29.129 kb on - strand, within B158DRAFT_0029 at 29.129 kb on - strand, within B158DRAFT_0029 at 29.131 kb on + strand, within B158DRAFT_0029 at 29.131 kb on + strand, within B158DRAFT_0029 at 29.131 kb on + strand, within B158DRAFT_0029 at 29.131 kb on + strand, within B158DRAFT_0029 at 29.131 kb on + strand, within B158DRAFT_0029 at 29.139 kb on - strand, within B158DRAFT_0029 at 29.139 kb on - strand, within B158DRAFT_0029 at 29.277 kb on + strand, within B158DRAFT_0029 at 29.302 kb on + strand, within B158DRAFT_0029 at 29.302 kb on + strand, within B158DRAFT_0029 at 29.302 kb on + strand, within B158DRAFT_0029 at 29.302 kb on + strand, within B158DRAFT_0029 at 29.302 kb on + strand, within B158DRAFT_0029 at 29.310 kb on - strand, within B158DRAFT_0029 at 29.310 kb on - strand, within B158DRAFT_0029 at 29.310 kb on - strand, within B158DRAFT_0029 at 29.310 kb on - strand, within B158DRAFT_0029 at 29.310 kb on - strand, within B158DRAFT_0029 at 29.310 kb on - strand, within B158DRAFT_0029 at 29.349 kb on - strand, within B158DRAFT_0029 at 29.390 kb on + strand, within B158DRAFT_0029 at 29.438 kb on + strand, within B158DRAFT_0029 at 29.450 kb on + strand, within B158DRAFT_0029 at 29.450 kb on + strand, within B158DRAFT_0029 at 29.453 kb on - strand, within B158DRAFT_0029 at 29.459 kb on + strand at 29.459 kb on + strand at 29.472 kb on - strand at 29.515 kb on - strand at 29.528 kb on - strand at 29.533 kb on + strand at 29.533 kb on + strand at 29.541 kb on - strand at 29.557 kb on - strand at 29.782 kb on + strand at 29.795 kb on + strand at 29.841 kb on + strand at 29.851 kb on - strand at 29.905 kb on + strand, within B158DRAFT_0030 at 29.942 kb on - strand, within B158DRAFT_0030 at 30.060 kb on - strand, within B158DRAFT_0030 at 30.067 kb on + strand, within B158DRAFT_0030 at 30.077 kb on - strand, within B158DRAFT_0030 at 30.077 kb on - strand, within B158DRAFT_0030 at 30.143 kb on + strand, within B158DRAFT_0030 at 30.151 kb on - strand, within B158DRAFT_0030 at 30.395 kb on - strand, within B158DRAFT_0031 at 30.460 kb on + strand, within B158DRAFT_0031 at 30.800 kb on + strand at 30.909 kb on - strand, within B158DRAFT_0032 at 30.968 kb on + strand, within B158DRAFT_0032 at 31.064 kb on - strand, within B158DRAFT_0032 at 31.125 kb on - strand, within B158DRAFT_0032 at 31.125 kb on - strand, within B158DRAFT_0032 at 31.159 kb on - strand, within B158DRAFT_0032 at 31.178 kb on + strand, within B158DRAFT_0032
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Gentamicin 0.1 mg/ml remove 28,860 - -2.5 28,866 - -0.6 28,882 + +0.3 28,883 + -0.1 28,891 - -2.9 28,891 - +0.7 28,891 - -0.7 28,893 + +0.0 28,905 + -0.6 28,945 + +0.2 28,945 + -0.4 28,952 + +0.0 28,952 + -0.2 28,952 + +2.1 28,952 + +1.2 28,952 + -1.3 28,952 + -0.2 28,953 - -0.6 28,960 - -0.5 28,960 - -0.9 28,960 - -0.1 28,960 - -1.3 29,010 + -0.1 29,010 + +0.2 29,010 + +0.2 29,015 + -3.9 29,018 - -0.7 29,018 - +0.9 29,018 - +0.1 29,018 - +1.0 29,018 - +0.4 29,094 + B158DRAFT_0029 0.15 -1.2 29,129 - B158DRAFT_0029 0.23 -1.4 29,129 - B158DRAFT_0029 0.23 -0.6 29,131 + B158DRAFT_0029 0.23 +0.2 29,131 + B158DRAFT_0029 0.23 +1.0 29,131 + B158DRAFT_0029 0.23 -0.5 29,131 + B158DRAFT_0029 0.23 -1.0 29,131 + B158DRAFT_0029 0.23 -0.7 29,139 - B158DRAFT_0029 0.25 +0.8 29,139 - B158DRAFT_0029 0.25 -0.5 29,277 + B158DRAFT_0029 0.53 -0.3 29,302 + B158DRAFT_0029 0.58 -0.3 29,302 + B158DRAFT_0029 0.58 -0.0 29,302 + B158DRAFT_0029 0.58 -0.0 29,302 + B158DRAFT_0029 0.58 -0.5 29,302 + B158DRAFT_0029 0.58 -0.1 29,310 - B158DRAFT_0029 0.60 -1.0 29,310 - B158DRAFT_0029 0.60 -3.1 29,310 - B158DRAFT_0029 0.60 +0.1 29,310 - B158DRAFT_0029 0.60 +0.7 29,310 - B158DRAFT_0029 0.60 -3.7 29,310 - B158DRAFT_0029 0.60 +0.6 29,349 - B158DRAFT_0029 0.68 -0.2 29,390 + B158DRAFT_0029 0.76 -0.9 29,438 + B158DRAFT_0029 0.86 -0.4 29,450 + B158DRAFT_0029 0.89 -0.8 29,450 + B158DRAFT_0029 0.89 +0.5 29,453 - B158DRAFT_0029 0.89 -1.1 29,459 + +0.6 29,459 + -0.7 29,472 - +0.1 29,515 - -0.0 29,528 - +0.2 29,533 + -1.2 29,533 + +0.4 29,541 - +0.0 29,557 - -0.1 29,782 + -0.1 29,795 + -0.1 29,841 + -0.1 29,851 - -3.3 29,905 + B158DRAFT_0030 0.22 +0.5 29,942 - B158DRAFT_0030 0.30 -0.3 30,060 - B158DRAFT_0030 0.58 -0.8 30,067 + B158DRAFT_0030 0.60 -0.7 30,077 - B158DRAFT_0030 0.62 -0.6 30,077 - B158DRAFT_0030 0.62 -0.5 30,143 + B158DRAFT_0030 0.78 -0.5 30,151 - B158DRAFT_0030 0.80 -1.8 30,395 - B158DRAFT_0031 0.27 -0.7 30,460 + B158DRAFT_0031 0.39 -1.4 30,800 + +0.1 30,909 - B158DRAFT_0032 0.11 -1.8 30,968 + B158DRAFT_0032 0.17 +0.0 31,064 - B158DRAFT_0032 0.27 -0.4 31,125 - B158DRAFT_0032 0.33 -0.4 31,125 - B158DRAFT_0032 0.33 -0.3 31,159 - B158DRAFT_0032 0.36 -1.6 31,178 + B158DRAFT_0032 0.38 -1.4
Or see this region's nucleotide sequence